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B1H2Q2

- ZRAN1_XENTR

UniProt

B1H2Q2 - ZRAN1_XENTR

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Protein

Ubiquitin thioesterase zranb1

Gene

zranb1

Organism
Xenopus tropicalis (Western clawed frog) (Silurana tropicalis)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli

Functioni

Specifically hydrolyzes 'Lys-29'-linked and 'Lys-33'-linked diubiquitin. Also cleaves 'Lys-63'-linked chains, but with 40-fold less efficiency compared to 'Lys-29'-linked ones. Positive regulator of the Wnt signaling pathway that deubiquitinates APC protein, a negative regulator of Wnt-mediated transcription. May play a role in the regulation of cell morphology and cytoskeletal organization. Required in the stress fiber dynamics and cell migration (By similarity).By similarity

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei436 – 4361NucleophileBy similarity
Active sitei578 – 5781Proton acceptorBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri3 – 3331RanBP2-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri79 – 10830RanBP2-type 2PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri143 – 17331RanBP2-type 3PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. ubiquitin-specific protease activity Source: UniProtKB
  2. ubiquitin thiolesterase activity Source: UniProtKB
  3. zinc ion binding Source: InterPro

GO - Biological processi

  1. cell migration Source: UniProtKB
  2. cytoskeleton organization Source: UniProtKB
  3. positive regulation of Wnt signaling pathway Source: UniProtKB
  4. protein K29-linked deubiquitination Source: UniProtKB
  5. protein K33-linked deubiquitination Source: UniProtKB
  6. protein K63-linked deubiquitination Source: UniProtKB
  7. regulation of cell morphogenesis Source: UniProtKB
  8. Wnt signaling pathway Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Ubl conjugation pathway, Wnt signaling pathway

Keywords - Ligandi

Metal-binding, Zinc

Protein family/group databases

MEROPSiC64.004.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin thioesterase zranb1 (EC:3.4.19.12)
Alternative name(s):
Zinc finger Ran-binding domain-containing protein 1
Gene namesi
Name:zranb1
OrganismiXenopus tropicalis (Western clawed frog) (Silurana tropicalis)
Taxonomic identifieri8364 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusSilurana
ProteomesiUP000008143: Unplaced

Organism-specific databases

XenbaseiXB-GENE-876399. zranb1.

Subcellular locationi

Cytoplasm By similarity. Nucleus By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB
  2. nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 701701Ubiquitin thioesterase zranb1PRO_0000361558Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi8364.ENSXETP00000050986.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati253 – 28331ANK 1Add
BLAST
Repeati306 – 33328ANK 2Add
BLAST
Domaini425 – 585161OTUPROSITE-ProRule annotationAdd
BLAST

Domaini

The OTU domain mediates the deubiquitinating activity.By similarity
The RanBP2-type zinc fingers mediate the specific interaction with 'Lys-63'-linked ubiquitin.By similarity

Sequence similaritiesi

Belongs to the peptidase C64 family.Curated
Contains 2 ANK repeats.Curated
Contains 1 OTU domain.PROSITE-ProRule annotation
Contains 3 RanBP2-type zinc fingers.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri3 – 3331RanBP2-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri79 – 10830RanBP2-type 2PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri143 – 17331RanBP2-type 3PROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

ANK repeat, Repeat, Zinc-finger

Phylogenomic databases

eggNOGiNOG253754.
HOGENOMiHOG000006743.
HOVERGENiHBG058978.
InParanoidiB1H2Q2.
KOiK11862.

Family and domain databases

InterProiIPR003323. OTU.
IPR001876. Znf_RanBP2.
[Graphical view]
PfamiPF02338. OTU. 1 hit.
PF00641. zf-RanBP. 2 hits.
[Graphical view]
SMARTiSM00547. ZnF_RBZ. 3 hits.
[Graphical view]
PROSITEiPS50802. OTU. 1 hit.
PS01358. ZF_RANBP2_1. 3 hits.
PS50199. ZF_RANBP2_2. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B1H2Q2-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTEHGIKWAC EYCTYENWPS AIKCTMCRAP RPSGAIITEE PFKNSTPDVG
60 70 80 90 100
SMERESGSPL ICPDSSARPR VKSSYSMETS TKWSCHMCTY LNWPRAIRCT
110 120 130 140 150
QCLSQRRTRS PTESPQSSGS SLRAIPSPID PCEEYNDRNK LNIKGQHWTC
160 170 180 190 200
SACTYENCAK AKKCVVCDHP TPNNMDAIEL ANTDEASSII NEQDRARWRG
210 220 230 240 250
GCSSSNSQRR SPPTSKRDSD MDFQRIELAG AVGSKEEFEL DLKKLKQIKN
260 270 280 290 300
RMRKTDWLFL NACVGVVEGD LSAVEAYKTS GGDIARQLSA DEVRLLNRPS
310 320 330 340 350
AFDVGYTLVH LSIRFQRQDM LAILLTEFSQ HAAKCIPAMV CPELTEQIRR
360 370 380 390 400
EIAASVHQRK GDFACYFLTD LVTFTLPADI EDLPPTVQEK LFDEVLDRDV
410 420 430 440 450
QKELEEESPI INWSLELGTR LDSRLYALWN RTAGDCLLDS VLQATWGIYD
460 470 480 490 500
KDSVLRKALH DSLHDCSHWF YSRWKEWESW YSQSFGLHFS LREEQWQEDW
510 520 530 540 550
AFILSLASQP GASLEQTHIF VLAHILRRPI IVYGVKYYKS FRGETLGYTR
560 570 580 590 600
FQGVYLPLLW EQSFCWKSPI ALGYTRGHFS ALVAMENDGF DNRGAGANLN
610 620 630 640 650
TDDDVTVTFL PLVDSERKLL HIHFLSAQEL GNEDQQEKLL REWMDCCVTE
660 670 680 690 700
GGVLVAMQKS SRRRNHPLVT QMVEKWLDGY RQIRPCTALS DGEEDEDDED

E
Length:701
Mass (Da):80,037
Last modified:April 29, 2008 - v1
Checksum:iDD3A68A82AF3DADE
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BC161086 mRNA. Translation: AAI61086.1.
RefSeqiNP_001116900.1. NM_001123428.1.
UniGeneiStr.29069.

Genome annotation databases

GeneIDi100144659.
KEGGixtr:100144659.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BC161086 mRNA. Translation: AAI61086.1 .
RefSeqi NP_001116900.1. NM_001123428.1.
UniGenei Str.29069.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 8364.ENSXETP00000050986.

Protein family/group databases

MEROPSi C64.004.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 100144659.
KEGGi xtr:100144659.

Organism-specific databases

CTDi 54764.
Xenbasei XB-GENE-876399. zranb1.

Phylogenomic databases

eggNOGi NOG253754.
HOGENOMi HOG000006743.
HOVERGENi HBG058978.
InParanoidi B1H2Q2.
KOi K11862.

Family and domain databases

InterProi IPR003323. OTU.
IPR001876. Znf_RanBP2.
[Graphical view ]
Pfami PF02338. OTU. 1 hit.
PF00641. zf-RanBP. 2 hits.
[Graphical view ]
SMARTi SM00547. ZnF_RBZ. 3 hits.
[Graphical view ]
PROSITEi PS50802. OTU. 1 hit.
PS01358. ZF_RANBP2_1. 3 hits.
PS50199. ZF_RANBP2_2. 3 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. NIH - Xenopus Gene Collection (XGC) project
    Submitted (MAR-2008) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Testis.

Entry informationi

Entry nameiZRAN1_XENTR
AccessioniPrimary (citable) accession number: B1H2Q2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 20, 2009
Last sequence update: April 29, 2008
Last modified: October 29, 2014
This is version 40 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3