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Protein

Leucine-rich repeat transmembrane protein FLRT3

Gene

Flrt3

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Functions in cell-cell adhesion, cell migration and axon guidance, exerting an attractive or repulsive role depending on its interaction partners. Plays a role in the spatial organization of brain neurons. Plays a role in vascular development in the retina (By similarity). Plays a role in cell-cell adhesion via its interaction with ADGRL3 and probably also other latrophilins that are expressed at the surface of adjacent cells (By similarity). Interaction with the intracellular domain of ROBO1 mediates axon attraction towards cells expressing NTN1. Mediates axon growth cone collapse and plays a repulsive role in neuron guidance via its interaction with UNC5B, and possibly also other UNC-5 family members (By similarity). Promotes neurite outgrowth (in vitro) (By similarity). Mediates cell-cell contacts that promote an increase both in neurite number and in neurite length (PubMed:15485775). Plays a role in the regulation of the density of glutamaergic synapses (PubMed:22405201). Plays a role in fibroblast growth factor-mediated signaling cascades. Required for normal morphogenesis during embryonic development, but not for normal embryonic patterning. Required for normal ventral closure, headfold fusion and definitive endoderm migration during embryonic development. Required for the formation of a normal basement membrane and the maintenance of a normal anterior visceral endoderm during embryonic development (By similarity).By similarity2 Publications

GO - Molecular functioni

GO - Biological processi

  • axon guidance Source: MGI
  • cell-cell adhesion via plasma-membrane adhesion molecules Source: UniProtKB
  • cytokine-mediated signaling pathway Source: GO_Central
  • embryonic morphogenesis Source: UniProtKB
  • fibroblast growth factor receptor signaling pathway Source: UniProtKB
  • head development Source: UniProtKB
  • heart development Source: UniProtKB
  • negative regulation of JAK-STAT cascade Source: GO_Central
  • negative regulation of protein kinase activity Source: GO_Central
  • neuron projection development Source: UniProtKB
  • neuron projection extension Source: UniProtKB
  • proepicardium cell migration involved in pericardium morphogenesis Source: UniProtKB
  • response to axon injury Source: UniProtKB
  • synapse assembly Source: UniProtKB
  • synapse organization Source: SynGO

Keywordsi

Molecular functionDevelopmental protein
Biological processCell adhesion

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine-rich repeat transmembrane protein FLRT3
Alternative name(s):
Fibronectin-like domain-containing leucine-rich transmembrane protein 3
Gene namesi
Name:Flrt3
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi1566005. Flrt3.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini29 – 528ExtracellularSequence analysisAdd BLAST500
Transmembranei529 – 549HelicalSequence analysisAdd BLAST21
Topological domaini550 – 649CytoplasmicSequence analysisAdd BLAST100

GO - Cellular componenti

  • axonal growth cone Source: UniProtKB
  • axon terminus Source: UniProtKB
  • cytoplasm Source: GO_Central
  • cytoplasmic vesicle Source: UniProtKB-KW
  • dendrite Source: UniProtKB-SubCell
  • endoplasmic reticulum membrane Source: UniProtKB-SubCell
  • extracellular region Source: UniProtKB-SubCell
  • focal adhesion Source: UniProtKB-SubCell
  • integral component of plasma membrane Source: UniProtKB
  • integral component of postsynaptic membrane Source: SynGO
  • postsynaptic density Source: SynGO
  • presynaptic membrane Source: UniProtKB-SubCell
  • synaptic membrane Source: UniProtKB

Keywords - Cellular componenti

Cell junction, Cell membrane, Cell projection, Cytoplasmic vesicle, Endoplasmic reticulum, Membrane, Postsynaptic cell membrane, Secreted, Synapse, Synaptosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 28Sequence analysisAdd BLAST28
ChainiPRO_000035267529 – 649Leucine-rich repeat transmembrane protein FLRT3Add BLAST621

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi31 ↔ 37By similarity
Disulfide bondi35 ↔ 44By similarity
Glycosylationi226N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi282N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi296N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi309 ↔ 334By similarity

Post-translational modificationi

N-glycosylated.By similarity
Proteolytic cleavage in the juxtamembrane region gives rise to a soluble ectodomain. Cleavage is probably effected by a metalloprotease.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiB1H234.
PRIDEiB1H234.

PTM databases

iPTMnetiB1H234.
PhosphoSitePlusiB1H234.

Expressioni

Tissue specificityi

Detected in brain (at protein level) (PubMed:22405201). Detected in brain neurons, especially in basal ganglia, hippocampus dentate gyrus and CA3 region, cerebellum and in olfactory bulb (PubMed:14706654, PubMed:15485775).3 Publications

Developmental stagei

At 12 dpc, detected in the developing brain, eye, lateral lip of the dermomyotome, somites and branchial arch.1 Publication

Inductioni

Up-regulated in neurons in dorsal root ganglia in response to peripheral nerve injury (at protein level) (PubMed:15485775). Up-regulated in neurons in dorsal root ganglia in response to peripheral nerve injury (PubMed:15485775).1 Publication

Gene expression databases

GenevisibleiB1H234. RN.

Interactioni

Subunit structurei

Monomer and homodimer. Self-associates (via leucine-rich repeats), giving rise to homooligomers. Interacts with FGFR1 (By similarity). Interacts (via extracellular domain) with ADGRL1/LPHN1 and ADGRL3 (via olfactomedin-like domain) (PubMed:22405201). Interacts (via extracellular domain) with LPHN2 (via olfactomedin-like domain). Interacts (via extracellular domain) with UNC5B (via Ig domain). May also interact (via extracellular domain) with UNC5A and UNC5C. Interacts (via extracellular domain) with UNC5D (via extracellular domain). Identified in complexes composed of FLRT3, ADGRL3 and UNC5B, respectively FLRT3, ADGRL3 and UNC5D (By similarity). Interacts (via cytoplasmic domain) with ROBO1 (By similarity).By similarity1 Publication

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000006454.

Structurei

3D structure databases

ProteinModelPortaliB1H234.
SMRiB1H234.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini29 – 58LRRNTSequence analysisAdd BLAST30
Repeati59 – 80LRR 1Sequence analysisAdd BLAST22
Repeati84 – 104LRR 2Sequence analysisAdd BLAST21
Repeati105 – 126LRR 3Sequence analysisAdd BLAST22
Repeati129 – 150LRR 4Sequence analysisAdd BLAST22
Repeati155 – 176LRR 5Sequence analysisAdd BLAST22
Repeati177 – 197LRR 6Sequence analysisAdd BLAST21
Repeati200 – 220LRR 7Sequence analysisAdd BLAST21
Repeati226 – 247LRR 8Sequence analysisAdd BLAST22
Repeati248 – 269LRR 9Sequence analysisAdd BLAST22
Repeati272 – 293LRR 10Sequence analysisAdd BLAST22
Domaini305 – 357LRRCTSequence analysisAdd BLAST53
Domaini405 – 504Fibronectin type-IIIPROSITE-ProRule annotationAdd BLAST100

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni38 – 67Interaction with ADGRL3By similarityAdd BLAST30

Keywords - Domaini

Leucine-rich repeat, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IIEA. Eukaryota.
COG4886. LUCA.
HOGENOMiHOG000290188.
HOVERGENiHBG051629.
InParanoidiB1H234.
KOiK16362.
OrthoDBiEOG091G05YU.
PhylomeDBiB1H234.
TreeFamiTF331598.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
3.80.10.10. 4 hits.
InterProiView protein in InterPro
IPR000483. Cys-rich_flank_reg_C.
IPR003961. FN3_dom.
IPR013783. Ig-like_fold.
IPR032675. L_dom-like.
IPR001611. Leu-rich_rpt.
IPR003591. Leu-rich_rpt_typical-subtyp.
IPR000372. LRRNT.
PfamiView protein in Pfam
PF13855. LRR_8. 2 hits.
SMARTiView protein in SMART
SM00369. LRR_TYP. 7 hits.
SM00082. LRRCT. 1 hit.
SM00013. LRRNT. 1 hit.
SUPFAMiSSF52058. SSF52058. 2 hits.
PROSITEiView protein in PROSITE
PS50853. FN3. 1 hit.
PS51450. LRR. 8 hits.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

B1H234-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MISPAWSLFL IGTKIGLFFQ VAPLSVMAKS CPSVCRCDAG FIYCNDRSLT
60 70 80 90 100
SIPVGIPEDA TTLYLQNNQI NNVGIPSDLK NLLKVQRIYL YHNSLDEFPT
110 120 130 140 150
NLPKYVKELH LQENNIRTIT YDSLSKIPYL EELHLDDNSV SAVSIEEGAF
160 170 180 190 200
RDSNYLRLLF LSRNHLSTIP GGLPRTIEEL RLDDNRISTI SSPSLHGLTS
210 220 230 240 250
LKRLVLDGNL LNNHGLGDKV FFNLVNLTEL SLVRNSLTAA PVNLPGTSLR
260 270 280 290 300
KLYLQDNHIN RVPPNAFSYL RQLYRLDMSN NNLSNLPQGI FDDLDNITQL
310 320 330 340 350
ILRNNPWYCG CKMKWVRDWL QSLPVKVNVR GLMCQAPEKV RGMAIKDLSA
360 370 380 390 400
ELFDCKDSGI VSTVQITTAI PNTAYPAQGQ WPAPVTKQPD IKNPKLTKDQ
410 420 430 440 450
RTTGSPSRKT ILITVKSVTP DTIHISWRLA LPMTALRLSW LKLGHSPAFG
460 470 480 490 500
SITETIVTGE RSEYLVTALE PESPYRVCMV PMETSNLYLF DETPVCIETQ
510 520 530 540 550
TAPLRMYNPT TTLNREQEKE PYKNPNLPLA AIIGGAVALV SIALLALVCW
560 570 580 590 600
YVHRNGSLFS RNCAYSKGRR RKDDYAEAGT KKDNSILEIR ETSFQMLPIS
610 620 630 640
NEPISKEEFV IHTIFPPNGM NLYKNNLSES SSNRSYRDSG IPDLDHSHS
Length:649
Mass (Da):72,911
Last modified:April 29, 2008 - v1
Checksum:i15DF42A55DD1F12C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CH473949 Genomic DNA. Translation: EDL80327.1.
BC160843 mRNA. Translation: AAI60843.1.
RefSeqiNP_001119763.1. NM_001126291.1.
UniGeneiRn.220263.

Genome annotation databases

GeneIDi366205.
KEGGirno:366205.
UCSCiRGD:1566005. rat.

Similar proteinsi

Entry informationi

Entry nameiFLRT3_RAT
AccessioniPrimary (citable) accession number: B1H234
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 14, 2008
Last sequence update: April 29, 2008
Last modified: August 30, 2017
This is version 79 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome