B1H1P9 (PPAL_XENLA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Lysosomal acid phosphatase
Short name=LAP
EC=3.1.3.2

Gene names

Name:

acp2

Organism

Xenopus laevis (African clawed frog)

Taxonomic identifier

8355 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Amphibia › Batrachia › Anura › Mesobatrachia › Pipoidea › Pipidae › Xenopodinae › Xenopus › Xenopus

Protein attributes

Sequence length	432 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	A phosphate monoester + H2O = an alcohol + phosphate.
Subcellular location	Lysosome membrane; Single-pass membrane protein; Lumenal side By similarity. Lysosome lumen By similarity. Note: The soluble form arises by proteolytic processing of the membrane-bound form By similarity.
Post-translational modification	The membrane-bound form is converted to the soluble form by sequential proteolytic processing. First, the C-terminal cytoplasmic tail is removed. Cleavage by a lysosomal protease releases the soluble form in the lysosome lumen By similarity.
Sequence similarities	Belongs to the histidine acid phosphatase family.

Ontologies

Keywords
Cellular component	Lysosome Membrane
Domain	Signal Transmembrane Transmembrane helix
Molecular function	Hydrolase
PTM	Disulfide bond Glycoprotein
Gene Ontology (GO)
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW lysosomal lumen Inferred from electronic annotation. Source: UniProtKB-SubCell lysosomal membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	acid phosphatase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 32	32	Potential
	Chain	33 – 432	400	Lysosomal acid phosphatase		PRO_0000356291
Regions
	Topological domain	33 – 384	352	Lumenal Potential
	Transmembrane	385 – 405	21	Helical; Potential
	Topological domain	406 – 432	27	Cytoplasmic Potential
Sites
	Active site	44	1	Nucleophile By similarity
	Active site	290	1	Proton donor By similarity
Amino acid modifications
	Glycosylation	94	1	N-linked (GlcNAc...) Potential
	Glycosylation	135	1	N-linked (GlcNAc...) Potential
	Glycosylation	179	1	N-linked (GlcNAc...) Potential
	Glycosylation	193	1	N-linked (GlcNAc...) Potential
	Glycosylation	269	1	N-linked (GlcNAc...) Potential
	Glycosylation	325	1	N-linked (GlcNAc...) Potential
	Glycosylation	334	1	N-linked (GlcNAc...) Potential
	Disulfide bond	161 ↔ 373		By similarity
	Disulfide bond	214 ↔ 313		By similarity
	Disulfide bond	348 ↔ 352		By similarity

Sequences

	Sequence		Length	Mass (Da)
	B1H1P9 [UniParc]. Last modified April 29, 2008. Version 1. Checksum: 7EB42A5E3837E245	FASTA	432	48,243
	10 20 30 40 50 60 MADGSCLGSG PQLGLIALLV VLLFSAVPLA QSRELRFVTL VYRHGDRSPV HGYPTDVHKE 70 80 90 100 110 120 SVWPQGYGQL TQVGMKQHWD LGQELRARYK GFLNESYNRH EIYVRSTDVD RTLMSAEANL 130 140 150 160 170 180 AGLYPPEGPQ IFNPNITWQP IPIHTIPESE DQLLKFPISP CPAYVKLQEE TRQSAEYINM 190 200 210 220 230 240 TTTYKAFLQM VANKTGLSDC TLESVWSVYD TLFCEKTHNF SLPTWATADV LSKLNKLKDF 250 260 270 280 290 300 SFVFLFGVHE RVKKARLQGG VLVDQILKNM TAAANNASNG LKLLAYSAHD STLGALQLAL 310 320 330 340 350 360 DVYNGKQAPY ASCHIFELYK EDSGNFTVQM YFRNESGKTP YPVSLPGCAH ACPLQDFQSL 370 380 390 400 410 420 LQPILAQDWE EECQTTSFIM TEETIIGLTI GAIALFIIIV VLMLLSCNEP KDDGYQHVSD 430 EGDDHETKGL AM

References

[1]	NIH - Xenopus Gene Collection (XGC) project Submitted (MAR-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Liver.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC160691 mRNA. Translation: AAI60691.1.
RefSeq	NP_001121203.1. NM_001127731.1.
UniGene	Xl.71198.
3D structure databases
ProteinModelPortal	B1H1P9.
SMR	B1H1P9. Positions 33-375.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	100158274.
KEGG	xla:100158274.
Organism-specific databases
CTD	53.
Xenbase	XB-GENE-6253082. acp2.
Phylogenomic databases
HOVERGEN	HBG002203.
Family and domain databases
InterPro	IPR000560. His_Pase_superF_clade-2. [Graphical view]
KO	K14410.
Pfam	PF00328. His_Phos_2. 1 hit. [Graphical view]
PROSITE	PS00616. HIS_ACID_PHOSPHAT_1. 1 hit. PS00778. HIS_ACID_PHOSPHAT_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PPAL_XENLA

Accession

Primary (citable) accession number: B1H1P9

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 16, 2008
Last sequence update:	April 29, 2008
Last modified:	November 16, 2011
This is version 21 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program