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Protein

Lysosomal acid phosphatase

Gene

acp2

Organism
Xenopus laevis (African clawed frog)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

A phosphate monoester + H2O = an alcohol + phosphate.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei44NucleophileBy similarity1
Active sitei290Proton donorBy similarity1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Names & Taxonomyi

Protein namesi
Recommended name:
Lysosomal acid phosphatase (EC:3.1.3.2)
Short name:
LAP
Gene namesi
Name:acp2
OrganismiXenopus laevis (African clawed frog)
Taxonomic identifieri8355 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

Organism-specific databases

XenbaseiXB-GENE-6253082. acp2.

Subcellular locationi

  • Lysosome membrane By similarity; Single-pass membrane protein Sequence analysis; Lumenal side By similarity
  • Lysosome lumen By similarity

  • Note: The soluble form arises by proteolytic processing of the membrane-bound form.By similarity

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini33 – 384LumenalSequence analysisAdd BLAST352
Transmembranei385 – 405HelicalSequence analysisAdd BLAST21
Topological domaini406 – 432CytoplasmicSequence analysisAdd BLAST27

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Lysosome, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 32Sequence analysisAdd BLAST32
ChainiPRO_000035629133 – 432Lysosomal acid phosphataseAdd BLAST400

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi94N-linked (GlcNAc...)Sequence analysis1
Glycosylationi135N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi161 ↔ 373By similarity
Glycosylationi179N-linked (GlcNAc...)Sequence analysis1
Glycosylationi193N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi214 ↔ 313By similarity
Glycosylationi269N-linked (GlcNAc...)Sequence analysis1
Glycosylationi325N-linked (GlcNAc...)Sequence analysis1
Glycosylationi334N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi348 ↔ 352By similarity

Post-translational modificationi

The membrane-bound form is converted to the soluble form by sequential proteolytic processing. First, the C-terminal cytoplasmic tail is removed. Cleavage by a lysosomal protease releases the soluble form in the lysosome lumen (By similarity).By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Structurei

3D structure databases

ProteinModelPortaliB1H1P9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the histidine acid phosphatase family.Curated

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

HOVERGENiHBG002203.
KOiK14410.

Family and domain databases

CDDicd07061. HP_HAP_like. 1 hit.
Gene3Di3.40.50.1240. 1 hit.
InterProiIPR033379. Acid_Pase_AS.
IPR000560. His_Pase_clade-2.
IPR029033. His_PPase_superfam.
[Graphical view]
PfamiPF00328. His_Phos_2. 1 hit.
[Graphical view]
SUPFAMiSSF53254. SSF53254. 1 hit.
PROSITEiPS00616. HIS_ACID_PHOSPHAT_1. 1 hit.
PS00778. HIS_ACID_PHOSPHAT_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

B1H1P9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADGSCLGSG PQLGLIALLV VLLFSAVPLA QSRELRFVTL VYRHGDRSPV
60 70 80 90 100
HGYPTDVHKE SVWPQGYGQL TQVGMKQHWD LGQELRARYK GFLNESYNRH
110 120 130 140 150
EIYVRSTDVD RTLMSAEANL AGLYPPEGPQ IFNPNITWQP IPIHTIPESE
160 170 180 190 200
DQLLKFPISP CPAYVKLQEE TRQSAEYINM TTTYKAFLQM VANKTGLSDC
210 220 230 240 250
TLESVWSVYD TLFCEKTHNF SLPTWATADV LSKLNKLKDF SFVFLFGVHE
260 270 280 290 300
RVKKARLQGG VLVDQILKNM TAAANNASNG LKLLAYSAHD STLGALQLAL
310 320 330 340 350
DVYNGKQAPY ASCHIFELYK EDSGNFTVQM YFRNESGKTP YPVSLPGCAH
360 370 380 390 400
ACPLQDFQSL LQPILAQDWE EECQTTSFIM TEETIIGLTI GAIALFIIIV
410 420 430
VLMLLSCNEP KDDGYQHVSD EGDDHETKGL AM
Length:432
Mass (Da):48,243
Last modified:April 29, 2008 - v1
Checksum:i7EB42A5E3837E245
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC160691 mRNA. Translation: AAI60691.1.
RefSeqiNP_001121203.1. NM_001127731.1.
UniGeneiXl.71198.

Genome annotation databases

GeneIDi100158274.
KEGGixla:100158274.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC160691 mRNA. Translation: AAI60691.1.
RefSeqiNP_001121203.1. NM_001127731.1.
UniGeneiXl.71198.

3D structure databases

ProteinModelPortaliB1H1P9.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi100158274.
KEGGixla:100158274.

Organism-specific databases

CTDi53.
XenbaseiXB-GENE-6253082. acp2.

Phylogenomic databases

HOVERGENiHBG002203.
KOiK14410.

Family and domain databases

CDDicd07061. HP_HAP_like. 1 hit.
Gene3Di3.40.50.1240. 1 hit.
InterProiIPR033379. Acid_Pase_AS.
IPR000560. His_Pase_clade-2.
IPR029033. His_PPase_superfam.
[Graphical view]
PfamiPF00328. His_Phos_2. 1 hit.
[Graphical view]
SUPFAMiSSF53254. SSF53254. 1 hit.
PROSITEiPS00616. HIS_ACID_PHOSPHAT_1. 1 hit.
PS00778. HIS_ACID_PHOSPHAT_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPPAL_XENLA
AccessioniPrimary (citable) accession number: B1H1P9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 16, 2008
Last sequence update: April 29, 2008
Last modified: October 5, 2016
This is version 36 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.