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B1H1P9 (PPAL_XENLA) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 26. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Lysosomal acid phosphatase

Short name=LAP
EC=3.1.3.2
Gene names
Name:acp2
OrganismXenopus laevis (African clawed frog)
Taxonomic identifier8355 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

Protein attributes

Sequence length432 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

A phosphate monoester + H2O = an alcohol + phosphate.

Subcellular location

Lysosome membrane; Single-pass membrane protein; Lumenal side By similarity. Lysosome lumen By similarity. Note: The soluble form arises by proteolytic processing of the membrane-bound form By similarity.

Post-translational modification

The membrane-bound form is converted to the soluble form by sequential proteolytic processing. First, the C-terminal cytoplasmic tail is removed. Cleavage by a lysosomal protease releases the soluble form in the lysosome lumen By similarity.

Sequence similarities

Belongs to the histidine acid phosphatase family.

Ontologies

Keywords
   Cellular componentLysosome
Membrane
   DomainSignal
Transmembrane
Transmembrane helix
   Molecular functionHydrolase
   PTMDisulfide bond
Glycoprotein
Gene Ontology (GO)
   Cellular_componentintegral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

lysosomal lumen

Inferred from electronic annotation. Source: UniProtKB-SubCell

lysosomal membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionacid phosphatase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3232 Potential
Chain33 – 432400Lysosomal acid phosphatase
PRO_0000356291

Regions

Topological domain33 – 384352Lumenal Potential
Transmembrane385 – 40521Helical; Potential
Topological domain406 – 43227Cytoplasmic Potential

Sites

Active site441Nucleophile By similarity
Active site2901Proton donor By similarity

Amino acid modifications

Glycosylation941N-linked (GlcNAc...) Potential
Glycosylation1351N-linked (GlcNAc...) Potential
Glycosylation1791N-linked (GlcNAc...) Potential
Glycosylation1931N-linked (GlcNAc...) Potential
Glycosylation2691N-linked (GlcNAc...) Potential
Glycosylation3251N-linked (GlcNAc...) Potential
Glycosylation3341N-linked (GlcNAc...) Potential
Disulfide bond161 ↔ 373 By similarity
Disulfide bond214 ↔ 313 By similarity
Disulfide bond348 ↔ 352 By similarity

Sequences

Sequence LengthMass (Da)Tools
B1H1P9 [UniParc].

Last modified April 29, 2008. Version 1.
Checksum: 7EB42A5E3837E245

FASTA43248,243
        10         20         30         40         50         60 
MADGSCLGSG PQLGLIALLV VLLFSAVPLA QSRELRFVTL VYRHGDRSPV HGYPTDVHKE 

        70         80         90        100        110        120 
SVWPQGYGQL TQVGMKQHWD LGQELRARYK GFLNESYNRH EIYVRSTDVD RTLMSAEANL 

       130        140        150        160        170        180 
AGLYPPEGPQ IFNPNITWQP IPIHTIPESE DQLLKFPISP CPAYVKLQEE TRQSAEYINM 

       190        200        210        220        230        240 
TTTYKAFLQM VANKTGLSDC TLESVWSVYD TLFCEKTHNF SLPTWATADV LSKLNKLKDF 

       250        260        270        280        290        300 
SFVFLFGVHE RVKKARLQGG VLVDQILKNM TAAANNASNG LKLLAYSAHD STLGALQLAL 

       310        320        330        340        350        360 
DVYNGKQAPY ASCHIFELYK EDSGNFTVQM YFRNESGKTP YPVSLPGCAH ACPLQDFQSL 

       370        380        390        400        410        420 
LQPILAQDWE EECQTTSFIM TEETIIGLTI GAIALFIIIV VLMLLSCNEP KDDGYQHVSD 

       430 
EGDDHETKGL AM 

« Hide

References

[1]NIH - Xenopus Gene Collection (XGC) project
Submitted (MAR-2008) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Liver.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BC160691 mRNA. Translation: AAI60691.1.
RefSeqNP_001121203.1. NM_001127731.1.
UniGeneXl.71198.

3D structure databases

ProteinModelPortalB1H1P9.
SMRB1H1P9. Positions 33-375.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID100158274.
KEGGxla:100158274.

Organism-specific databases

CTD53.
XenbaseXB-GENE-6253082. acp2.

Phylogenomic databases

HOVERGENHBG002203.
KOK14410.

Family and domain databases

InterProIPR000560. His_Pase_superF_clade-2.
[Graphical view]
PfamPF00328. His_Phos_2. 1 hit.
[Graphical view]
PROSITEPS00616. HIS_ACID_PHOSPHAT_1. 1 hit.
PS00778. HIS_ACID_PHOSPHAT_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePPAL_XENLA
AccessionPrimary (citable) accession number: B1H1P9
Entry history
Integrated into UniProtKB/Swiss-Prot: December 16, 2008
Last sequence update: April 29, 2008
Last modified: April 16, 2014
This is version 26 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families