ID   B1H1J6_DANRE            Unreviewed;       500 AA.
AC   B1H1J6; A0A8M1NIF4;
DT   29-APR-2008, integrated into UniProtKB/TrEMBL.
DT   29-APR-2008, sequence version 1.
DT   27-MAR-2024, entry version 114.
DE   SubName: Full=Dihydropyrimidinase {ECO:0000313|Ensembl:ENSDARP00000103150, ECO:0000313|RefSeq:NP_001116535.1};
DE            EC=3.5.2.2 {ECO:0000313|RefSeq:NP_001116535.1};
DE   SubName: Full=Dpys protein {ECO:0000313|EMBL:AAI60631.1};
GN   Name=dpys {ECO:0000313|EMBL:AAI60631.1,
GN   ECO:0000313|Ensembl:ENSDARP00000103150,
GN   ECO:0000313|RefSeq:NP_001116535.1,
GN   ECO:0000313|ZFIN:ZDB-GENE-070508-1};
OS   Danio rerio (Zebrafish) (Brachydanio rerio).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC   Danionidae; Danioninae; Danio.
OX   NCBI_TaxID=7955 {ECO:0000313|EMBL:AAI60631.1};
RN   [1] {ECO:0000313|EMBL:AAI60631.1}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Whole body {ECO:0000313|EMBL:AAI60631.1};
RG   NIH - Zebrafish Gene Collection (ZGC) project;
RL   Submitted (MAR-2008) to the EMBL/GenBank/DDBJ databases.
RN   [2] {ECO:0000313|Ensembl:ENSDARP00000103150}
RP   IDENTIFICATION.
RC   STRAIN=Tuebingen {ECO:0000313|Ensembl:ENSDARP00000103150};
RG   Ensembl;
RL   Submitted (FEB-2012) to UniProtKB.
RN   [3] {ECO:0000313|Ensembl:ENSDARP00000103150, ECO:0000313|Proteomes:UP000000437}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Tuebingen {ECO:0000313|Ensembl:ENSDARP00000103150};
RX   PubMed=23594743; DOI=10.1038/nature12111;
RG   Genome Reference Consortium Zebrafish;
RA   Howe K., Clark M.D., Torroja C.F., Torrance J., Berthelot C., Muffato M.,
RA   Collins J.E., Humphray S., McLaren K., Matthews L., McLaren S., Sealy I.,
RA   Caccamo M., Churcher C., Scott C., Barrett J.C., Koch R., Rauch G.J.,
RA   White S., Chow W., Kilian B., Quintais L.T., Guerra-Assuncao J.A., Zhou Y.,
RA   Gu Y., Yen J., Vogel J.H., Eyre T., Redmond S., Banerjee R., Chi J., Fu B.,
RA   Langley E., Maguire S.F., Laird G.K., Lloyd D., Kenyon E., Donaldson S.,
RA   Sehra H., Almeida-King J., Loveland J., Trevanion S., Jones M., Quail M.,
RA   Willey D., Hunt A., Burton J., Sims S., McLay K., Plumb B., Davis J.,
RA   Clee C., Oliver K., Clark R., Riddle C., Elliot D., Eliott D.,
RA   Threadgold G., Harden G., Ware D., Begum S., Mortimore B., Mortimer B.,
RA   Kerry G., Heath P., Phillimore B., Tracey A., Corby N., Dunn M.,
RA   Johnson C., Wood J., Clark S., Pelan S., Griffiths G., Smith M.,
RA   Glithero R., Howden P., Barker N., Lloyd C., Stevens C., Harley J.,
RA   Holt K., Panagiotidis G., Lovell J., Beasley H., Henderson C., Gordon D.,
RA   Auger K., Wright D., Collins J., Raisen C., Dyer L., Leung K.,
RA   Robertson L., Ambridge K., Leongamornlert D., McGuire S., Gilderthorp R.,
RA   Griffiths C., Manthravadi D., Nichol S., Barker G., Whitehead S., Kay M.,
RA   Brown J., Murnane C., Gray E., Humphries M., Sycamore N., Barker D.,
RA   Saunders D., Wallis J., Babbage A., Hammond S., Mashreghi-Mohammadi M.,
RA   Barr L., Martin S., Wray P., Ellington A., Matthews N., Ellwood M.,
RA   Woodmansey R., Clark G., Cooper J., Cooper J., Tromans A., Grafham D.,
RA   Skuce C., Pandian R., Andrews R., Harrison E., Kimberley A., Garnett J.,
RA   Fosker N., Hall R., Garner P., Kelly D., Bird C., Palmer S., Gehring I.,
RA   Berger A., Dooley C.M., Ersan-Urun Z., Eser C., Geiger H., Geisler M.,
RA   Karotki L., Kirn A., Konantz J., Konantz M., Oberlander M.,
RA   Rudolph-Geiger S., Teucke M., Lanz C., Raddatz G., Osoegawa K., Zhu B.,
RA   Rapp A., Widaa S., Langford C., Yang F., Schuster S.C., Carter N.P.,
RA   Harrow J., Ning Z., Herrero J., Searle S.M., Enright A., Geisler R.,
RA   Plasterk R.H., Lee C., Westerfield M., de Jong P.J., Zon L.I.,
RA   Postlethwait J.H., Nusslein-Volhard C., Hubbard T.J., Roest Crollius H.,
RA   Rogers J., Stemple D.L.;
RT   "The zebrafish reference genome sequence and its relationship to the human
RT   genome.";
RL   Nature 496:498-503(2013).
RN   [4] {ECO:0000313|RefSeq:NP_001116535.1}
RP   NUCLEOTIDE SEQUENCE.
RX   PubMed=26469318;
RA   Elkon R., Milon B., Morrison L., Shah M., Vijayakumar S., Racherla M.,
RA   Leitch C.C., Silipino L., Hadi S., Weiss-Gayet M., Barras E., Schmid C.D.,
RA   Ait-Lounis A., Barnes A., Song Y., Eisenman D.J., Eliyahu E.,
RA   Frolenkov G.I., Strome S.E., Durand B., Zaghloul N.A., Jones S.M.,
RA   Reith W., Hertzano R.;
RT   "RFX transcription factors are essential for hearing in mice.";
RL   Nat. Commun. 6:8549-8549(2015).
RN   [5] {ECO:0000313|RefSeq:NP_001116535.1}
RP   NUCLEOTIDE SEQUENCE.
RX   PubMed=27296292;
RA   Lagman D., Franzen I.E., Eggert J., Larhammar D., Abalo X.M.;
RT   "Evolution and expression of the phosphodiesterase 6 genes unveils
RT   vertebrate novelty to control photosensitivity.";
RL   BMC Evol. Biol. 16:124-124(2016).
RN   [6] {ECO:0000313|RefSeq:NP_001116535.1}
RP   NUCLEOTIDE SEQUENCE.
RX   PubMed=28982700; DOI=10.1242/bio.030254;
RA   Takada N., Omae M., Sagawa F., Chi N.C., Endo S., Kozawa S., Sato T.N.;
RT   "Re-evaluating the functional landscape of the cardiovascular system during
RT   development.";
RL   Biol. Open 6:1756-1770(2017).
RN   [7] {ECO:0000313|RefSeq:NP_001116535.1}
RP   NUCLEOTIDE SEQUENCE.
RX   PubMed=28252024;
RA   Bayes A., Collins M.O., Reig-Viader R., Gou G., Goulding D., Izquierdo A.,
RA   Choudhary J.S., Emes R.D., Grant S.G.;
RT   "Evolution of complexity in the zebrafish synapse proteome.";
RL   Nat. Commun. 8:14613-14613(2017).
RN   [8] {ECO:0000313|RefSeq:NP_001116535.1}
RP   NUCLEOTIDE SEQUENCE.
RX   PubMed=30465971;
RA   Zheng Y., Yuan J., Meng S., Chen J., Gu Z.;
RT   "Testicular transcriptome alterations in zebrafish (Danio rerio) exposure
RT   to 17beta-estradiol.";
RL   Chemosphere 218:14-25(2019).
RN   [9] {ECO:0000313|RefSeq:NP_001116535.1}
RP   IDENTIFICATION.
RG   RefSeq;
RL   Submitted (NOV-2023) to UniProtKB.
CC   -!- PTM: Carbamylation allows a single lysine to coordinate two divalent
CC       metal cations. {ECO:0000256|PIRSR:PIRSR611778-50}.
CC   -!- SIMILARITY: Belongs to the metallo-dependent hydrolases superfamily.
CC       Hydantoinase/dihydropyrimidinase family.
CC       {ECO:0000256|ARBA:ARBA00008829}.
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DR   EMBL; CU695177; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; LO018239; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; BC160631; AAI60631.1; -; mRNA.
DR   RefSeq; NP_001116535.1; NM_001123063.1.
DR   STRING; 7955.ENSDARP00000103150; -.
DR   MEROPS; M38.973; -.
DR   PaxDb; 7955-ENSDARP00000103150; -.
DR   Ensembl; ENSDART00000114795.4; ENSDARP00000103150.2; ENSDARG00000079543.5.
DR   GeneID; 100144569; -.
DR   KEGG; dre:100144569; -.
DR   AGR; ZFIN:ZDB-GENE-070508-1; -.
DR   CTD; 1807; -.
DR   ZFIN; ZDB-GENE-070508-1; dpys.
DR   eggNOG; KOG2584; Eukaryota.
DR   HOGENOM; CLU_015572_2_2_1; -.
DR   OMA; SAETHHM; -.
DR   OrthoDB; 1772494at2759; -.
DR   TreeFam; TF314706; -.
DR   Reactome; R-DRE-73621; Pyrimidine catabolism.
DR   Proteomes; UP000000437; Chromosome 16.
DR   Bgee; ENSDARG00000079543; Expressed in liver and 12 other cell types or tissues.
DR   GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR   GO; GO:0004157; F:dihydropyrimidinase activity; IBA:GO_Central.
DR   GO; GO:0006208; P:pyrimidine nucleobase catabolic process; IBA:GO_Central.
DR   CDD; cd01314; D-HYD; 1.
DR   Gene3D; 3.20.20.140; Metal-dependent hydrolases; 1.
DR   Gene3D; 2.30.40.10; Urease, subunit C, domain 1; 1.
DR   InterPro; IPR006680; Amidohydro-rel.
DR   InterPro; IPR011778; Hydantoinase/dihydroPyrase.
DR   InterPro; IPR011059; Metal-dep_hydrolase_composite.
DR   InterPro; IPR032466; Metal_Hydrolase.
DR   NCBIfam; TIGR02033; D-hydantoinase; 1.
DR   PANTHER; PTHR11647:SF50; DIHYDROPYRIMIDINASE; 1.
DR   PANTHER; PTHR11647; HYDRANTOINASE/DIHYDROPYRIMIDINASE FAMILY MEMBER; 1.
DR   Pfam; PF01979; Amidohydro_1; 1.
DR   SUPFAM; SSF51338; Composite domain of metallo-dependent hydrolases; 2.
DR   SUPFAM; SSF51556; Metallo-dependent hydrolases; 1.
PE   1: Evidence at protein level;
KW   Proteomics identification {ECO:0007829|PeptideAtlas:B1H1J6};
KW   Reference proteome {ECO:0000313|Proteomes:UP000000437}.
FT   DOMAIN          54..443
FT                   /note="Amidohydrolase-related"
FT                   /evidence="ECO:0000259|Pfam:PF01979"
FT   MOD_RES         155
FT                   /note="N6-carboxylysine"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR611778-50"
SQ   SEQUENCE   500 AA;  54684 MW;  C20D7669283B7A74 CRC64;
     MTAQSCILIK GGKVVNEDFS VMADVYIEDG VISGVGTDLQ VAAGTRVIDA THKLVLPGGI
     DSHTHMELSF MGTTTADDFQ TGTQAAVAGG TTMILDFVIA QKGCSLLEAY ERWRRTADPK
     VCCDYSLHMA VTWWDDTVKK EMETLVSEKG INSFKMFMAY KDLYMLQDLE LYAVFSTCKE
     IGAIAQVHAE NGDLIAEGAK RMLSLGITGP EGHEMCRPEE VEAEATQRAI TIAHATNCPL
     YVVHVMSKSA AKVVANARRN GRLVFGEPIA AGLGTDGTHY WHENWAHAAG FVMGPPLRPD
     PSTPGYLMDL LANDDLSVTG TDNCTFSVCQ KALGKDDFTK IPNGVNGVED RMSVIWEKGV
     HSGKMDENRF VAVTSTNAAK IFNLYPRKGR IAKGSDADLV IWDPKASRVI SAKTHHQAVD
     YNIFEGMLCH GVPVVTISRG KVVYENGKLF TKPGEGRYIP RKPFAEFVYK RINQREKVGT
     PSAVCREPYT GEIISIGLKQ
//