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Protein

Glutamate--tRNA ligase

Gene

gltX

Organism
Uncultured termite group 1 bacterium phylotype Rs-D17
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).UniRule annotation

Catalytic activityi

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi110 – 1101ZincUniRule annotation
Metal bindingi112 – 1121ZincUniRule annotation
Metal bindingi137 – 1371ZincUniRule annotation
Metal bindingi139 – 1391ZincUniRule annotation
Binding sitei257 – 2571ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciUTER471821:GJAD-580-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate--tRNA ligaseUniRule annotation (EC:6.1.1.17UniRule annotation)
Alternative name(s):
Glutamyl-tRNA synthetaseUniRule annotation
Short name:
GluRSUniRule annotation
Gene namesi
Name:gltXUniRule annotation
Ordered Locus Names:TGRD_542
OrganismiUncultured termite group 1 bacterium phylotype Rs-D17
Taxonomic identifieri471821 [NCBI]
Taxonomic lineageiBacteriaElusimicrobiaenvironmental samples
ProteomesiUP000001691 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 494494Glutamate--tRNA ligasePRO_1000090118Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi471821.TGRD_542.

Structurei

3D structure databases

ProteinModelPortaliB1H0J3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi10 – 2011"HIGH" regionAdd
BLAST
Motifi254 – 2585"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0008.
HOGENOMiHOG000252722.
KOiK01885.
OMAiNWINSQY.
OrthoDBiEOG6DRPF7.

Family and domain databases

Gene3Di1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_00022_B. Glu_tRNA_synth_B.
InterProiIPR008925. aa-tRNA-synth_I_codon-bd.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR001412. aa-tRNA-synth_I_CS.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERiPTHR10119. PTHR10119. 1 hit.
PfamiPF00749. tRNA-synt_1c. 1 hit.
[Graphical view]
PRINTSiPR00987. TRNASYNTHGLU.
SUPFAMiSSF48163. SSF48163. 1 hit.
TIGRFAMsiTIGR00464. gltX_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B1H0J3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSGIRVRFAP SPTGDLHIGG VRTALFNWLF AKNKGGKFIL RIEDTDETRS
60 70 80 90 100
TGESAKVILD AMKWLELEWN EGPGNENLKY SPYSQMKRKE RGIYRKYADE
110 120 130 140 150
LVAKELAYPC YCTPEEIDVM RKKAKADKLP PKYDGKCRHL TPGQRKQKEI
160 170 180 190 200
EGKKAVVRFK MFGSGTTVLN DIIRGRVKFD NSLLDDFVIM KASGVPAYNF
210 220 230 240 250
ACVVDDYLME ITHVLRGDDH ISNAPRQMHI YNALGWEMPE FAHMSMILGA
260 270 280 290 300
GGARLSKRHG HTSVLEYRKE GYLREALINY LALLGWSTED SQQIFTIEEL
310 320 330 340 350
KQKFSVERCG ISPSIFDPAK LLWLNGEKIR SKTPQQVYEL FIDWLKYTGN
360 370 380 390 400
EKLTETWDVE LLKKALILEH DKIKLLKDIP SLVDFFFTKN VDYKEEAVKK
410 420 430 440 450
TLLSEKSKDI AKLVLIESAA RLPNQLDFSA ISLEQYARNL AAEKNIKTGQ
460 470 480 490
VFHPIRVAIS GRMQGPGLFQ MMEVMGKEDV VRRINVAINK FFQK
Length:494
Mass (Da):56,547
Last modified:April 29, 2008 - v1
Checksum:iE1A64E5136CA754A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP009510 Genomic DNA. Translation: BAG14025.1.
RefSeqiWP_015423550.1. NC_020419.1.
YP_001956486.1. NC_020419.1.

Genome annotation databases

EnsemblBacteriaiBAG14025; BAG14025; TGRD_542.
GeneIDi6373192.
KEGGirsd:TGRD_542.
PATRICi38571400. VBIUncTer52152_0883.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP009510 Genomic DNA. Translation: BAG14025.1.
RefSeqiWP_015423550.1. NC_020419.1.
YP_001956486.1. NC_020419.1.

3D structure databases

ProteinModelPortaliB1H0J3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi471821.TGRD_542.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAG14025; BAG14025; TGRD_542.
GeneIDi6373192.
KEGGirsd:TGRD_542.
PATRICi38571400. VBIUncTer52152_0883.

Phylogenomic databases

eggNOGiCOG0008.
HOGENOMiHOG000252722.
KOiK01885.
OMAiNWINSQY.
OrthoDBiEOG6DRPF7.

Enzyme and pathway databases

BioCyciUTER471821:GJAD-580-MONOMER.

Family and domain databases

Gene3Di1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_00022_B. Glu_tRNA_synth_B.
InterProiIPR008925. aa-tRNA-synth_I_codon-bd.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR001412. aa-tRNA-synth_I_CS.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERiPTHR10119. PTHR10119. 1 hit.
PfamiPF00749. tRNA-synt_1c. 1 hit.
[Graphical view]
PRINTSiPR00987. TRNASYNTHGLU.
SUPFAMiSSF48163. SSF48163. 1 hit.
TIGRFAMsiTIGR00464. gltX_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Complete genome of the uncultured termite group 1 bacteria in a single host protist cell."
    Hongoh Y., Sharma V.K., Prakash T., Noda S., Taylor T.D., Kudo T., Sakaki Y., Toyoda A., Hattori M., Ohkuma M.
    Proc. Natl. Acad. Sci. U.S.A. 105:5555-5560(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Entry informationi

Entry nameiSYE_UNCTG
AccessioniPrimary (citable) accession number: B1H0J3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: April 29, 2008
Last modified: July 22, 2015
This is version 53 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.