Reviewed, UniProtKB/Swiss-Prot B1H0I0 (RIBBA_UNCTG)

Names and origin

Protein names

Recommended name:
    Riboflavin biosynthesis protein ribBA
Including the following 2 domains:
    1- Recommended name:
            3,4-dihydroxy-2-butanone 4-phosphate synthase
                Short name=DHBP synthase
              EC=4.1.99.12
    2- Recommended name:
            GTP cyclohydrolase-2
              EC=3.5.4.25
        Alternative name(s):
            GTP cyclohydrolase II

Gene names

Name:	ribBA
Ordered Locus Names:	TGRD_529

Organism

Uncultured termite group 1 bacterium phylotype Rs-D17 [Complete proteome] [HAMAP]

Taxonomic identifier

471821 [NCBI]

Taxonomic lineage

Bacteria › Elusimicrobia › environmental samples

Protein attributes

Sequence length	403 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the conversion of D-ribulose 5-phosphate to formate and 3,4-dihydroxy-2-butanone 4-phosphate By similarity. Catalyzes the conversion of GTP to 2,5-diamino-6-ribosylamino-4(3H)-pyrimidinone 5'-phosphate (DARP), formate and pyrophosphate By similarity.
Catalytic activity	D-ribulose 5-phosphate = formate + L-3,4-dihydroxybutan-2-one 4-phosphate. HAMAP MF_01283 GTP + 3 H2O = formate + 2,5-diamino-6-hydroxy-4-(5-phosphoribosylamino)pyrimidine + diphosphate. HAMAP MF_01283
Cofactor	Binds 2 divalent metal cations per subunit. Magnesium or manganese By similarity. Binds 1 zinc ion per subunit By similarity.
Pathway	Cofactor biosynthesis; riboflavin biosynthesis; 2-hydroxy-3-oxobutyl phosphate from D-ribulose 5-phosphate: step 1/1. HAMAP MF_01283 Cofactor biosynthesis; riboflavin biosynthesis; 5-amino-6-(D-ribitylamino)uracil from GTP: step 1/4. HAMAP MF_01283
Sequence similarities	In the N-terminal section; belongs to the DHBP synthase family. In the C-terminal section; belongs to the GTP cyclohydrolase II family.

Ontologies

Keywords
Biological process	Riboflavin biosynthesis
Ligand	GTP-binding Magnesium Manganese Metal-binding Nucleotide-binding Zinc
Molecular function	Hydrolase Lyase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	riboflavin biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	3,4-dihydroxy-2-butanone-4-phosphate synthase activity Inferred from electronic annotation. Source: HAMAP GTP binding Inferred from electronic annotation. Source: UniProtKB-KW GTP cyclohydrolase II activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP manganese ion binding Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 403	403	Riboflavin biosynthesis protein ribBA HAMAP MF_01283		PRO_1000140369
Regions
	Nucleotide binding	255 – 259	5	GTP By similarity
	Nucleotide binding	298 – 300	3	GTP By similarity
	Region	1 – 204	204	DHBP synthase HAMAP MF_01283
	Region	30 – 31	2	D-ribulose 5-phosphate binding By similarity
	Region	143 – 147	5	D-ribulose 5-phosphate binding By similarity
	Region	205 – 403	199	GTP cyclohydrolase II HAMAP MF_01283
Sites
	Active site	332	1	Proton acceptor; for GTP cyclohydrolase activity Potential
	Active site	334	1	Nucleophile; for GTP cyclohydrolase activity By similarity
	Metal binding	31	1	Magnesium or manganese 1 By similarity
	Metal binding	31	1	Magnesium or manganese 2 By similarity
	Metal binding	146	1	Magnesium or manganese 2 By similarity
	Metal binding	260	1	Zinc; catalytic By similarity
	Metal binding	271	1	Zinc; catalytic By similarity
	Metal binding	273	1	Zinc; catalytic By similarity
	Binding site	35	1	D-ribulose 5-phosphate By similarity
	Binding site	167	1	D-ribulose 5-phosphate By similarity
	Binding site	276	1	GTP By similarity
	Binding site	320	1	GTP By similarity
	Binding site	355	1	GTP By similarity
	Binding site	360	1	GTP By similarity
	Site	129	1	Essential for DHBP synthase activity By similarity
	Site	167	1	Essential for DHBP synthase activity By similarity

Sequences

	Sequence		Length	Mass (Da)
	B1H0I0-1 [UniParc]. Last modified April 29, 2008. Version 1. Checksum: 9FF1E288DA7226B2	FASTA	403	44,853
	10 20 30 40 50 60 MKNKVFASIG DAVKDIKVGR MVIVVDDPGR ENEGDLICAA EKASPEVINF MTKYARGLIC 70 80 90 100 110 120 VPMKHERLKE LEIENMVEKP TEKKGCSFTV SVDYKIGTTT GISAYDRSVT VRKLIDKTAK 130 140 150 160 170 180 HEDFARPGHI FPLRCKEGGV LARTGHTEAA VDLVRLAGFY PAGIICEIMN NDGTMARMSD 190 200 210 220 230 240 LIKFAKKHDL HIITIGELVN YRRRTEKFIS EIVNVDLPTR YGDFKLVLFE DLITKDSHIA 250 260 270 280 290 300 VVKGVVKNRQ NVLVRVHSSC ETGDIFHSLR CDCGDQLETA LKAVGEAEQG VVLYIHQEGR 310 320 330 340 350 360 GIGLANKLKA YRLQEKGMDT VEANKALGFD PDLRDYGIGA QMLSELGIKS INLMTNNPGK 370 380 390 400 INGLESYGLK ITKRVPLEIS PSKSNEKYLK TKKEKMGHML KKV

References

[1]

"Complete genome of the uncultured termite group 1 bacteria in a single host protist cell." Hongoh Y., Sharma V.K., Prakash T., Noda S., Taylor T.D., Kudo T., Sakaki Y., Toyoda A., Hattori M., Ohkuma M. Proc. Natl. Acad. Sci. U.S.A. 105:5555-5560(2008) [PubMed: 18391199] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AP009510 Genomic DNA. Translation: BAG14012.1.
RefSeq	YP_001956473.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	6373331.
GenomeReviews	Gene locus TGRD_529 in contig AP009510_GR.
KEGG	rsd:TGRD_529.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	HTEASLE.
Family and domain databases
HAMAP	MF_01283. [Tree]
InterPro	IPR017945. DHBP_synth_RibB-like_a/b_dom. IPR000422. DHBP_synthase_RibB. IPR000926. GTP_CycHdrlase_II. IPR016299. Riboflavin_synth_RibA. [Graphical view]
Gene3D	G3DSA:3.90.870.10. DHBP_synth_RibB-like_a/b_dom. 1 hit.
Pfam	PF00926. DHBP_synthase. 1 hit. PF00925. GTP_cyclohydro2. 1 hit. [Graphical view]
PIRSF	PIRSF001259. RibA. 1 hit.
ProDom	PD003034. DHBP_synthase. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00505. ribA. 1 hit. TIGR00506. ribB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RIBBA_UNCTG

Accession

Primary (citable) accession number: B1H0I0

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	April 29, 2008
Last modified:	November 3, 2009
This is version 13 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)