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B1B557

- NOSL_BOMMO

UniProt

B1B557 - NOSL_BOMMO

Protein

Nitric oxide synthase-like protein

Gene

NSL

Organism
Bombyx mori (Silk moth)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 43 (01 Oct 2014)
      Sequence version 1 (29 Apr 2008)
      Previous versions | rss
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    Functioni

    Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body.By similarity

    Catalytic activityi

    2 L-arginine + 3 NADPH + 4 O2 = 2 L-citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O.

    Cofactori

    Heme group.By similarity
    Binds 1 FAD.By similarity
    Binds 1 FMN.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi77 – 771Iron (heme axial ligand)By similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi426 – 4305FMNPROSITE-ProRule annotation
    Nucleotide bindingi561 – 59232FMNPROSITE-ProRule annotationAdd
    BLAST
    Nucleotide bindingi704 – 71512FADBy similarityAdd
    BLAST
    Nucleotide bindingi847 – 85711FADBy similarityAdd
    BLAST
    Nucleotide bindingi922 – 94019NADPBy similarityAdd
    BLAST
    Nucleotide bindingi1019 – 103416NADPBy similarityAdd
    BLAST

    GO - Molecular functioni

    1. flavin adenine dinucleotide binding Source: InterPro
    2. FMN binding Source: InterPro
    3. heme binding Source: InterPro
    4. iron ion binding Source: InterPro
    5. NADP binding Source: InterPro
    6. nitric-oxide synthase activity Source: UniProtKB-EC

    GO - Biological processi

    1. nitric oxide biosynthetic process Source: InterPro

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Ligandi

    Calmodulin-binding, FAD, Flavoprotein, FMN, Heme, Iron, Metal-binding, NADP

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Nitric oxide synthase-like protein (EC:1.14.13.39)
    Gene namesi
    Name:NSLImported
    OrganismiBombyx mori (Silk moth)
    Taxonomic identifieri7091 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaLepidopteraGlossataDitrysiaBombycoideaBombycidaeBombycinaeBombyx
    ProteomesiUP000005204: Unplaced

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 10971097Nitric oxide synthase-like proteinPRO_0000403314Add
    BLAST

    Structurei

    3D structure databases

    ProteinModelPortaliB1B557.
    SMRiB1B557. Positions 9-379, 417-493.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini420 – 615196Flavodoxin-likePROSITE-ProRule annotationAdd
    BLAST
    Domaini669 – 914246FAD-binding FR-typePROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni387 – 41024Calmodulin-bindingBy similarityAdd
    BLAST
    Regioni415 – 43420Tetrahydrobiopterin-bindingBy similarityAdd
    BLAST

    Sequence similaritiesi

    Belongs to the NOS family.Sequence Analysis
    Contains 1 FAD-binding FR-type domain.PROSITE-ProRule annotation
    Contains 1 flavodoxin-like domain.PROSITE-ProRule annotation

    Phylogenomic databases

    KOiK13253.
    OMAiMALYYLK.
    OrthoDBiEOG79SDW7.

    Family and domain databases

    Gene3Di1.20.990.10. 1 hit.
    3.40.50.360. 2 hits.
    3.90.340.10. 1 hit.
    InterProiIPR003097. FAD-binding_1.
    IPR017927. Fd_Rdtase_FAD-bd.
    IPR001094. Flavdoxin.
    IPR008254. Flavodoxin/NO_synth.
    IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
    IPR029039. Flavoprotein-like.
    IPR023173. NADPH_Cyt_P450_Rdtase_dom3.
    IPR012144. NOS_euk.
    IPR004030. NOS_N.
    IPR001433. OxRdtase_FAD/NAD-bd.
    IPR017938. Riboflavin_synthase-like_b-brl.
    [Graphical view]
    PfamiPF00667. FAD_binding_1. 1 hit.
    PF00258. Flavodoxin_1. 1 hit.
    PF00175. NAD_binding_1. 1 hit.
    PF02898. NO_synthase. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000333. NOS. 1 hit.
    PRINTSiPR00369. FLAVODOXIN.
    PR00371. FPNCR.
    SUPFAMiSSF52218. SSF52218. 1 hit.
    SSF56512. SSF56512. 1 hit.
    SSF63380. SSF63380. 1 hit.
    PROSITEiPS51384. FAD_FR. 1 hit.
    PS50902. FLAVODOXIN_LIKE. 1 hit.
    PS60001. NOS. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Note: Additional isoforms seem to exist.Curated

    Isoform 1Imported (identifier: B1B557-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MAIPGRGDVP RDPEEVLNDA KDFLGQYFAS IRRANTPAHE ARWKIVQEEV     50
    ATTGTYQLTT TELVFGAKLA WRNASRCIGR IQWSKLQVFD CRQVTTTSGM 100
    FEALCNHIKY STNKGNIRSA ITLFPQRTDG KHDYRIWNSQ LISYAGYRQP 150
    DGTVLGDPMH CEFTDLCLKL GWKPPRTAWD ILPLVLSANG KDPDYFEIPR 200
    ELVMEIHMTH PTFEWFKELE LRWYALPAVS SMRFDCGGIE FTANAFNGWY 250
    MSTEIGCRNF CDTNRLNVLE KIAQNMGLDT RTPVNLWKDK ALVEVNVAVL 300
    HSFQQHNATI VDHHTASESF IKHLDNENRL RSGCPADWIW IVPPMSSSIT 350
    PVFHQEMALY YLKPSYEYQE PAWKTHQWQK SKPITGKRPI NRKFHFKQIA 400
    RAVKFTSKLF GRALSKRIKA TVLYATETGK SEQYAKELGV IFGHAFNAQV 450
    HCMADYDITS IEHEALLLVV TSTFGNGDPP ENGVAFGEHL CEILYADQVG 500
    EDSTGNQMLT PKSFIKANSD IQRYTAGNPK KLNRLESLKG STTDATSIDS 550
    FGPLSNVRFA VFALGSSAYP NFCNFGKYVD KLLVDLGGER IHDLATGDEM 600
    CGQDQAFRKW ASSVFNVACE TFCLDDDETL QEAKRALGTV ALSEETVQFA 650
    RAGCRPATLH AALQKSLNKQ FVSCTVKANK DLGDASAERS TIFIDLEPKE 700
    EIKYNPGDHV GIIACNRKEL VESLLSRIKD VDDYDEPLQL QLLKETHTSS 750
    GLVKSWEPHE KLPIMSVREL FTRFLDITTP PTTILLQYLA TTCEDEEEKK 800
    QLNVLATDPG AYEDWRHFHF PTLPEVLDQF PSARPNASLL AALLSPLQPR 850
    FYSISSSPLA HAKRLHLTVA VVTYRTQDGE GPVHYGVCSN YLMERKPGDE 900
    VYLFIRSAPN FHLPQDLSVP LILIGPGTGI APFRGFWHHR RALQNSCSRT 950
    TTGPVWLFFG CRTKTMDLYR EEKEQALKEG VLSKVFLALS REKEVPKTYV 1000
    QEVAENVGAE IHDLLINKGA HFYVCGDCKM AEDVHQKLKG IVKKHGNMTD 1050
    EQVQNFMFML KEENRYHEDI FGITLRTAEV HSASRESARR NRVASQP 1097
    Length:1,097
    Mass (Da):124,059
    Last modified:April 29, 2008 - v1
    Checksum:iBE3639FFEA05DE35
    GO
    Isoform 2Imported (identifier: B1B557-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-231: Missing.

    Show »
    Length:866
    Mass (Da):97,550
    Checksum:iC37CF55A412E3D96
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti604 – 6041D → G in BAH23564. 1 PublicationCurated
    Sequence conflicti675 – 6751T → A in BAH23564. 1 PublicationCurated
    Sequence conflicti885 – 8851Y → F in BAH23564. 1 PublicationCurated
    Sequence conflicti998 – 9981T → M in BAH23564. 1 PublicationCurated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 231231Missing in isoform 2. 1 PublicationVSP_040368Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB360590 mRNA. Translation: BAG12563.1.
    AB485776 mRNA. Translation: BAH23564.1.
    RefSeqiNP_001116808.1. NM_001123336.1. [B1B557-1]
    UniGeneiBmo.16070.

    Genome annotation databases

    EnsemblMetazoaiBGIBMGA002937-RA; BGIBMGA002937-TA; BGIBMGA002937. [B1B557-1]
    GeneIDi100144542.
    KEGGibmor:100144542.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB360590 mRNA. Translation: BAG12563.1 .
    AB485776 mRNA. Translation: BAH23564.1 .
    RefSeqi NP_001116808.1. NM_001123336.1. [B1B557-1 ]
    UniGenei Bmo.16070.

    3D structure databases

    ProteinModelPortali B1B557.
    SMRi B1B557. Positions 9-379, 417-493.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblMetazoai BGIBMGA002937-RA ; BGIBMGA002937-TA ; BGIBMGA002937 . [B1B557-1 ]
    GeneIDi 100144542.
    KEGGi bmor:100144542.

    Organism-specific databases

    CTDi 4843.

    Phylogenomic databases

    KOi K13253.
    OMAi MALYYLK.
    OrthoDBi EOG79SDW7.

    Family and domain databases

    Gene3Di 1.20.990.10. 1 hit.
    3.40.50.360. 2 hits.
    3.90.340.10. 1 hit.
    InterProi IPR003097. FAD-binding_1.
    IPR017927. Fd_Rdtase_FAD-bd.
    IPR001094. Flavdoxin.
    IPR008254. Flavodoxin/NO_synth.
    IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
    IPR029039. Flavoprotein-like.
    IPR023173. NADPH_Cyt_P450_Rdtase_dom3.
    IPR012144. NOS_euk.
    IPR004030. NOS_N.
    IPR001433. OxRdtase_FAD/NAD-bd.
    IPR017938. Riboflavin_synthase-like_b-brl.
    [Graphical view ]
    Pfami PF00667. FAD_binding_1. 1 hit.
    PF00258. Flavodoxin_1. 1 hit.
    PF00175. NAD_binding_1. 1 hit.
    PF02898. NO_synthase. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000333. NOS. 1 hit.
    PRINTSi PR00369. FLAVODOXIN.
    PR00371. FPNCR.
    SUPFAMi SSF52218. SSF52218. 1 hit.
    SSF56512. SSF56512. 1 hit.
    SSF63380. SSF63380. 1 hit.
    PROSITEi PS51384. FAD_FR. 1 hit.
    PS50902. FLAVODOXIN_LIKE. 1 hit.
    PS60001. NOS. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Positional cloning of a Bombyx wingless locus flugellos (fl) reveals a crucial role for fringe that is specific for wing morphogenesis."
      Sato K., Matsuoka-Matsunaga T., Futahashi R., Kojima T., Mita K., Banno Y., Fujiwara H.
      Genetics 179:875-885(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
      Tissue: Wing imaginal diskImported.
    2. "Identification of nitric-oxide synthase from Bombyx mori."
      Nagaoka S., Takata Y.
      Submitted (FEB-2009) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
      Tissue: Reproductive systemImported.

    Entry informationi

    Entry nameiNOSL_BOMMO
    AccessioniPrimary (citable) accession number: B1B557
    Secondary accession number(s): B9X250
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 11, 2011
    Last sequence update: April 29, 2008
    Last modified: October 1, 2014
    This is version 43 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3