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B1B557

- NOSL_BOMMO

UniProt

B1B557 - NOSL_BOMMO

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Protein

Nitric oxide synthase-like protein

Gene

NSL

Organism
Bombyx mori (Silk moth)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli

Functioni

Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body.By similarity

Catalytic activityi

2 L-arginine + 3 NADPH + 4 O2 = 2 L-citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O.

Cofactori

Protein has several cofactor binding sites:
  • hemeBy similarity
  • FADBy similarityNote: Binds 1 FAD.By similarity
  • FMNBy similarityNote: Binds 1 FMN.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi77 – 771Iron (heme axial ligand)By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi426 – 4305FMNPROSITE-ProRule annotation
Nucleotide bindingi561 – 59232FMNPROSITE-ProRule annotationAdd
BLAST
Nucleotide bindingi704 – 71512FADBy similarityAdd
BLAST
Nucleotide bindingi847 – 85711FADBy similarityAdd
BLAST
Nucleotide bindingi922 – 94019NADPBy similarityAdd
BLAST
Nucleotide bindingi1019 – 103416NADPBy similarityAdd
BLAST

GO - Molecular functioni

  1. flavin adenine dinucleotide binding Source: InterPro
  2. FMN binding Source: InterPro
  3. heme binding Source: InterPro
  4. iron ion binding Source: InterPro
  5. NADP binding Source: InterPro
  6. nitric-oxide synthase activity Source: UniProtKB-EC

GO - Biological processi

  1. nitric oxide biosynthetic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

Calmodulin-binding, FAD, Flavoprotein, FMN, Heme, Iron, Metal-binding, NADP

Names & Taxonomyi

Protein namesi
Recommended name:
Nitric oxide synthase-like protein (EC:1.14.13.39)
Gene namesi
Name:NSLImported
OrganismiBombyx mori (Silk moth)
Taxonomic identifieri7091 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaLepidopteraGlossataDitrysiaBombycoideaBombycidaeBombycinaeBombyx
ProteomesiUP000005204: Unplaced

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10971097Nitric oxide synthase-like proteinPRO_0000403314Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliB1B557.
SMRiB1B557. Positions 9-379, 417-493.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini420 – 615196Flavodoxin-likePROSITE-ProRule annotationAdd
BLAST
Domaini669 – 914246FAD-binding FR-typePROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni387 – 41024Calmodulin-bindingBy similarityAdd
BLAST
Regioni415 – 43420Tetrahydrobiopterin-bindingBy similarityAdd
BLAST

Sequence similaritiesi

Belongs to the NOS family.Sequence Analysis
Contains 1 FAD-binding FR-type domain.PROSITE-ProRule annotation
Contains 1 flavodoxin-like domain.PROSITE-ProRule annotation

Phylogenomic databases

InParanoidiB1B557.
KOiK13253.
OMAiMALYYLK.
OrthoDBiEOG79SDW7.

Family and domain databases

Gene3Di1.20.990.10. 1 hit.
3.40.50.360. 2 hits.
3.90.340.10. 1 hit.
InterProiIPR003097. FAD-binding_1.
IPR017927. Fd_Rdtase_FAD-bd.
IPR001094. Flavdoxin.
IPR008254. Flavodoxin/NO_synth.
IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
IPR029039. Flavoprotein-like.
IPR023173. NADPH_Cyt_P450_Rdtase_dom3.
IPR012144. NOS_euk.
IPR004030. NOS_N.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PfamiPF00667. FAD_binding_1. 1 hit.
PF00258. Flavodoxin_1. 1 hit.
PF00175. NAD_binding_1. 1 hit.
PF02898. NO_synthase. 1 hit.
[Graphical view]
PIRSFiPIRSF000333. NOS. 1 hit.
PRINTSiPR00369. FLAVODOXIN.
PR00371. FPNCR.
SUPFAMiSSF52218. SSF52218. 1 hit.
SSF56512. SSF56512. 1 hit.
SSF63380. SSF63380. 1 hit.
PROSITEiPS51384. FAD_FR. 1 hit.
PS50902. FLAVODOXIN_LIKE. 1 hit.
PS60001. NOS. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Note: Additional isoforms seem to exist.Curated

Isoform 1Imported (identifier: B1B557-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAIPGRGDVP RDPEEVLNDA KDFLGQYFAS IRRANTPAHE ARWKIVQEEV
60 70 80 90 100
ATTGTYQLTT TELVFGAKLA WRNASRCIGR IQWSKLQVFD CRQVTTTSGM
110 120 130 140 150
FEALCNHIKY STNKGNIRSA ITLFPQRTDG KHDYRIWNSQ LISYAGYRQP
160 170 180 190 200
DGTVLGDPMH CEFTDLCLKL GWKPPRTAWD ILPLVLSANG KDPDYFEIPR
210 220 230 240 250
ELVMEIHMTH PTFEWFKELE LRWYALPAVS SMRFDCGGIE FTANAFNGWY
260 270 280 290 300
MSTEIGCRNF CDTNRLNVLE KIAQNMGLDT RTPVNLWKDK ALVEVNVAVL
310 320 330 340 350
HSFQQHNATI VDHHTASESF IKHLDNENRL RSGCPADWIW IVPPMSSSIT
360 370 380 390 400
PVFHQEMALY YLKPSYEYQE PAWKTHQWQK SKPITGKRPI NRKFHFKQIA
410 420 430 440 450
RAVKFTSKLF GRALSKRIKA TVLYATETGK SEQYAKELGV IFGHAFNAQV
460 470 480 490 500
HCMADYDITS IEHEALLLVV TSTFGNGDPP ENGVAFGEHL CEILYADQVG
510 520 530 540 550
EDSTGNQMLT PKSFIKANSD IQRYTAGNPK KLNRLESLKG STTDATSIDS
560 570 580 590 600
FGPLSNVRFA VFALGSSAYP NFCNFGKYVD KLLVDLGGER IHDLATGDEM
610 620 630 640 650
CGQDQAFRKW ASSVFNVACE TFCLDDDETL QEAKRALGTV ALSEETVQFA
660 670 680 690 700
RAGCRPATLH AALQKSLNKQ FVSCTVKANK DLGDASAERS TIFIDLEPKE
710 720 730 740 750
EIKYNPGDHV GIIACNRKEL VESLLSRIKD VDDYDEPLQL QLLKETHTSS
760 770 780 790 800
GLVKSWEPHE KLPIMSVREL FTRFLDITTP PTTILLQYLA TTCEDEEEKK
810 820 830 840 850
QLNVLATDPG AYEDWRHFHF PTLPEVLDQF PSARPNASLL AALLSPLQPR
860 870 880 890 900
FYSISSSPLA HAKRLHLTVA VVTYRTQDGE GPVHYGVCSN YLMERKPGDE
910 920 930 940 950
VYLFIRSAPN FHLPQDLSVP LILIGPGTGI APFRGFWHHR RALQNSCSRT
960 970 980 990 1000
TTGPVWLFFG CRTKTMDLYR EEKEQALKEG VLSKVFLALS REKEVPKTYV
1010 1020 1030 1040 1050
QEVAENVGAE IHDLLINKGA HFYVCGDCKM AEDVHQKLKG IVKKHGNMTD
1060 1070 1080 1090
EQVQNFMFML KEENRYHEDI FGITLRTAEV HSASRESARR NRVASQP
Length:1,097
Mass (Da):124,059
Last modified:April 29, 2008 - v1
Checksum:iBE3639FFEA05DE35
GO
Isoform 2Imported (identifier: B1B557-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-231: Missing.

Show »
Length:866
Mass (Da):97,550
Checksum:iC37CF55A412E3D96
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti604 – 6041D → G in BAH23564. 1 PublicationCurated
Sequence conflicti675 – 6751T → A in BAH23564. 1 PublicationCurated
Sequence conflicti885 – 8851Y → F in BAH23564. 1 PublicationCurated
Sequence conflicti998 – 9981T → M in BAH23564. 1 PublicationCurated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 231231Missing in isoform 2. 1 PublicationVSP_040368Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB360590 mRNA. Translation: BAG12563.1.
AB485776 mRNA. Translation: BAH23564.1.
RefSeqiNP_001116808.1. NM_001123336.1. [B1B557-1]
UniGeneiBmo.16070.

Genome annotation databases

EnsemblMetazoaiBGIBMGA002937-RA; BGIBMGA002937-TA; BGIBMGA002937. [B1B557-1]
GeneIDi100144542.
KEGGibmor:100144542.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB360590 mRNA. Translation: BAG12563.1 .
AB485776 mRNA. Translation: BAH23564.1 .
RefSeqi NP_001116808.1. NM_001123336.1. [B1B557-1 ]
UniGenei Bmo.16070.

3D structure databases

ProteinModelPortali B1B557.
SMRi B1B557. Positions 9-379, 417-493.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblMetazoai BGIBMGA002937-RA ; BGIBMGA002937-TA ; BGIBMGA002937 . [B1B557-1 ]
GeneIDi 100144542.
KEGGi bmor:100144542.

Organism-specific databases

CTDi 4843.

Phylogenomic databases

InParanoidi B1B557.
KOi K13253.
OMAi MALYYLK.
OrthoDBi EOG79SDW7.

Family and domain databases

Gene3Di 1.20.990.10. 1 hit.
3.40.50.360. 2 hits.
3.90.340.10. 1 hit.
InterProi IPR003097. FAD-binding_1.
IPR017927. Fd_Rdtase_FAD-bd.
IPR001094. Flavdoxin.
IPR008254. Flavodoxin/NO_synth.
IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
IPR029039. Flavoprotein-like.
IPR023173. NADPH_Cyt_P450_Rdtase_dom3.
IPR012144. NOS_euk.
IPR004030. NOS_N.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view ]
Pfami PF00667. FAD_binding_1. 1 hit.
PF00258. Flavodoxin_1. 1 hit.
PF00175. NAD_binding_1. 1 hit.
PF02898. NO_synthase. 1 hit.
[Graphical view ]
PIRSFi PIRSF000333. NOS. 1 hit.
PRINTSi PR00369. FLAVODOXIN.
PR00371. FPNCR.
SUPFAMi SSF52218. SSF52218. 1 hit.
SSF56512. SSF56512. 1 hit.
SSF63380. SSF63380. 1 hit.
PROSITEi PS51384. FAD_FR. 1 hit.
PS50902. FLAVODOXIN_LIKE. 1 hit.
PS60001. NOS. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Positional cloning of a Bombyx wingless locus flugellos (fl) reveals a crucial role for fringe that is specific for wing morphogenesis."
    Sato K., Matsuoka-Matsunaga T., Futahashi R., Kojima T., Mita K., Banno Y., Fujiwara H.
    Genetics 179:875-885(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Tissue: Wing imaginal diskImported.
  2. "Identification of nitric-oxide synthase from Bombyx mori."
    Nagaoka S., Takata Y.
    Submitted (FEB-2009) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
    Tissue: Reproductive systemImported.

Entry informationi

Entry nameiNOSL_BOMMO
AccessioniPrimary (citable) accession number: B1B557
Secondary accession number(s): B9X250
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 11, 2011
Last sequence update: April 29, 2008
Last modified: November 26, 2014
This is version 45 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3