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B1B557

- NOSL_BOMMO

UniProt

B1B557 - NOSL_BOMMO

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Protein

Nitric oxide synthase-like protein

Gene
NSL
Organism
Bombyx mori (Silk moth)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at transcript leveli

Functioni

Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body By similarity.

Catalytic activityi

2 L-arginine + 3 NADPH + 4 O2 = 2 L-citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O.

Cofactori

Heme group By similarity.By similarity
Binds 1 FAD By similarity.By similarity
Binds 1 FMN By similarity.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi77 – 771Iron (heme axial ligand) By similarityBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi426 – 4305FMN By similarity
Nucleotide bindingi561 – 59232FMN By similarityAdd
BLAST
Nucleotide bindingi704 – 71512FAD By similarityBy similarityAdd
BLAST
Nucleotide bindingi847 – 85711FAD By similarityBy similarityAdd
BLAST
Nucleotide bindingi922 – 94019NADP By similarityBy similarityAdd
BLAST
Nucleotide bindingi1019 – 103416NADP By similarityBy similarityAdd
BLAST

GO - Molecular functioni

  1. flavin adenine dinucleotide binding Source: InterPro
  2. FMN binding Source: InterPro
  3. heme binding Source: InterPro
  4. iron ion binding Source: InterPro
  5. NADP binding Source: InterPro
  6. nitric-oxide synthase activity Source: UniProtKB-EC

GO - Biological processi

  1. nitric oxide biosynthetic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

Calmodulin-binding, FAD, Flavoprotein, FMN, Heme, Iron, Metal-binding, NADP

Names & Taxonomyi

Protein namesi
Recommended name:
Nitric oxide synthase-like protein (EC:1.14.13.39)
Gene namesi
Name:NSL
OrganismiBombyx mori (Silk moth)
Taxonomic identifieri7091 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaLepidopteraGlossataDitrysiaBombycoideaBombycidaeBombycinaeBombyx
ProteomesiUP000005204: Unplaced

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10971097Nitric oxide synthase-like proteinPRO_0000403314Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliB1B557.
SMRiB1B557. Positions 9-379, 417-493.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini420 – 615196Flavodoxin-likeAdd
BLAST
Domaini669 – 914246FAD-binding FR-typeAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni387 – 41024Calmodulin-binding By similarityBy similarityAdd
BLAST
Regioni415 – 43420Tetrahydrobiopterin-binding By similarityBy similarityAdd
BLAST

Sequence similaritiesi

Belongs to the NOS family.

Phylogenomic databases

KOiK13253.
OMAiMALYYLK.
OrthoDBiEOG79SDW7.

Family and domain databases

Gene3Di1.20.990.10. 1 hit.
3.40.50.360. 2 hits.
3.90.340.10. 1 hit.
InterProiIPR003097. FAD-binding_1.
IPR017927. Fd_Rdtase_FAD-bd.
IPR001094. Flavdoxin.
IPR008254. Flavodoxin/NO_synth.
IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
IPR029039. Flavoprotein-like.
IPR023173. NADPH_Cyt_P450_Rdtase_dom3.
IPR012144. NOS_euk.
IPR004030. NOS_N.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PfamiPF00667. FAD_binding_1. 1 hit.
PF00258. Flavodoxin_1. 1 hit.
PF00175. NAD_binding_1. 1 hit.
PF02898. NO_synthase. 1 hit.
[Graphical view]
PIRSFiPIRSF000333. NOS. 1 hit.
PRINTSiPR00369. FLAVODOXIN.
PR00371. FPNCR.
SUPFAMiSSF52218. SSF52218. 1 hit.
SSF56512. SSF56512. 1 hit.
SSF63380. SSF63380. 1 hit.
PROSITEiPS51384. FAD_FR. 1 hit.
PS50902. FLAVODOXIN_LIKE. 1 hit.
PS60001. NOS. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Note: Additional isoforms seem to exist.

Isoform 1 (identifier: B1B557-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MAIPGRGDVP RDPEEVLNDA KDFLGQYFAS IRRANTPAHE ARWKIVQEEV     50
ATTGTYQLTT TELVFGAKLA WRNASRCIGR IQWSKLQVFD CRQVTTTSGM 100
FEALCNHIKY STNKGNIRSA ITLFPQRTDG KHDYRIWNSQ LISYAGYRQP 150
DGTVLGDPMH CEFTDLCLKL GWKPPRTAWD ILPLVLSANG KDPDYFEIPR 200
ELVMEIHMTH PTFEWFKELE LRWYALPAVS SMRFDCGGIE FTANAFNGWY 250
MSTEIGCRNF CDTNRLNVLE KIAQNMGLDT RTPVNLWKDK ALVEVNVAVL 300
HSFQQHNATI VDHHTASESF IKHLDNENRL RSGCPADWIW IVPPMSSSIT 350
PVFHQEMALY YLKPSYEYQE PAWKTHQWQK SKPITGKRPI NRKFHFKQIA 400
RAVKFTSKLF GRALSKRIKA TVLYATETGK SEQYAKELGV IFGHAFNAQV 450
HCMADYDITS IEHEALLLVV TSTFGNGDPP ENGVAFGEHL CEILYADQVG 500
EDSTGNQMLT PKSFIKANSD IQRYTAGNPK KLNRLESLKG STTDATSIDS 550
FGPLSNVRFA VFALGSSAYP NFCNFGKYVD KLLVDLGGER IHDLATGDEM 600
CGQDQAFRKW ASSVFNVACE TFCLDDDETL QEAKRALGTV ALSEETVQFA 650
RAGCRPATLH AALQKSLNKQ FVSCTVKANK DLGDASAERS TIFIDLEPKE 700
EIKYNPGDHV GIIACNRKEL VESLLSRIKD VDDYDEPLQL QLLKETHTSS 750
GLVKSWEPHE KLPIMSVREL FTRFLDITTP PTTILLQYLA TTCEDEEEKK 800
QLNVLATDPG AYEDWRHFHF PTLPEVLDQF PSARPNASLL AALLSPLQPR 850
FYSISSSPLA HAKRLHLTVA VVTYRTQDGE GPVHYGVCSN YLMERKPGDE 900
VYLFIRSAPN FHLPQDLSVP LILIGPGTGI APFRGFWHHR RALQNSCSRT 950
TTGPVWLFFG CRTKTMDLYR EEKEQALKEG VLSKVFLALS REKEVPKTYV 1000
QEVAENVGAE IHDLLINKGA HFYVCGDCKM AEDVHQKLKG IVKKHGNMTD 1050
EQVQNFMFML KEENRYHEDI FGITLRTAEV HSASRESARR NRVASQP 1097
Length:1,097
Mass (Da):124,059
Last modified:April 29, 2008 - v1
Checksum:iBE3639FFEA05DE35
GO
Isoform 2 (identifier: B1B557-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-231: Missing.

Show »
Length:866
Mass (Da):97,550
Checksum:iC37CF55A412E3D96
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 231231Missing in isoform 2. VSP_040368Add
BLAST

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti604 – 6041D → G in BAH23564. 1 Publication
Sequence conflicti675 – 6751T → A in BAH23564. 1 Publication
Sequence conflicti885 – 8851Y → F in BAH23564. 1 Publication
Sequence conflicti998 – 9981T → M in BAH23564. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB360590 mRNA. Translation: BAG12563.1.
AB485776 mRNA. Translation: BAH23564.1.
RefSeqiNP_001116808.1. NM_001123336.1. [B1B557-1]
UniGeneiBmo.16070.

Genome annotation databases

EnsemblMetazoaiBGIBMGA002937-RA; BGIBMGA002937-TA; BGIBMGA002937. [B1B557-1]
GeneIDi100144542.
KEGGibmor:100144542.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB360590 mRNA. Translation: BAG12563.1 .
AB485776 mRNA. Translation: BAH23564.1 .
RefSeqi NP_001116808.1. NM_001123336.1. [B1B557-1 ]
UniGenei Bmo.16070.

3D structure databases

ProteinModelPortali B1B557.
SMRi B1B557. Positions 9-379, 417-493.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblMetazoai BGIBMGA002937-RA ; BGIBMGA002937-TA ; BGIBMGA002937 . [B1B557-1 ]
GeneIDi 100144542.
KEGGi bmor:100144542.

Organism-specific databases

CTDi 4843.

Phylogenomic databases

KOi K13253.
OMAi MALYYLK.
OrthoDBi EOG79SDW7.

Family and domain databases

Gene3Di 1.20.990.10. 1 hit.
3.40.50.360. 2 hits.
3.90.340.10. 1 hit.
InterProi IPR003097. FAD-binding_1.
IPR017927. Fd_Rdtase_FAD-bd.
IPR001094. Flavdoxin.
IPR008254. Flavodoxin/NO_synth.
IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
IPR029039. Flavoprotein-like.
IPR023173. NADPH_Cyt_P450_Rdtase_dom3.
IPR012144. NOS_euk.
IPR004030. NOS_N.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view ]
Pfami PF00667. FAD_binding_1. 1 hit.
PF00258. Flavodoxin_1. 1 hit.
PF00175. NAD_binding_1. 1 hit.
PF02898. NO_synthase. 1 hit.
[Graphical view ]
PIRSFi PIRSF000333. NOS. 1 hit.
PRINTSi PR00369. FLAVODOXIN.
PR00371. FPNCR.
SUPFAMi SSF52218. SSF52218. 1 hit.
SSF56512. SSF56512. 1 hit.
SSF63380. SSF63380. 1 hit.
PROSITEi PS51384. FAD_FR. 1 hit.
PS50902. FLAVODOXIN_LIKE. 1 hit.
PS60001. NOS. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Positional cloning of a Bombyx wingless locus flugellos (fl) reveals a crucial role for fringe that is specific for wing morphogenesis."
    Sato K., Matsuoka-Matsunaga T., Futahashi R., Kojima T., Mita K., Banno Y., Fujiwara H.
    Genetics 179:875-885(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Tissue: Wing imaginal disk.
  2. "Identification of nitric-oxide synthase from Bombyx mori."
    Nagaoka S., Takata Y.
    Submitted (FEB-2009) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
    Tissue: Reproductive system.

Entry informationi

Entry nameiNOSL_BOMMO
AccessioniPrimary (citable) accession number: B1B557
Secondary accession number(s): B9X250
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 11, 2011
Last sequence update: April 29, 2008
Last modified: September 3, 2014
This is version 42 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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