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B1B557 (NOSL_BOMMO) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 39. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Nitric oxide synthase-like protein

EC=1.14.13.39
Gene names
Name:NSL
OrganismBombyx mori (Silk moth) [Reference proteome]
Taxonomic identifier7091 [NCBI]
Taxonomic lineageEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaLepidopteraGlossataDitrysiaBombycoideaBombycidaeBombycinaeBombyx

Protein attributes

Sequence length1097 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body By similarity.

Catalytic activity

2 L-arginine + 3 NADPH + 4 O2 = 2 L-citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O.

Cofactor

Heme group By similarity. UniProtKB P29475

Binds 1 FAD By similarity. UniProtKB P29475

Binds 1 FMN By similarity. UniProtKB P29475

Sequence similarities

Belongs to the NOS family.

Contains 1 FAD-binding FR-type domain.

Contains 1 flavodoxin-like domain.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Note: Additional isoforms seem to exist.
Isoform 1 (identifier: B1B557-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: B1B557-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-231: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 10971097Nitric oxide synthase-like protein
PRO_0000403314

Regions

Domain420 – 615196Flavodoxin-like
Domain669 – 914246FAD-binding FR-type
Nucleotide binding426 – 4305FMN By similarity
Nucleotide binding561 – 59232FMN By similarity
Nucleotide binding704 – 71512FAD By similarity UniProtKB P29475
Nucleotide binding847 – 85711FAD By similarity UniProtKB P29475
Nucleotide binding922 – 94019NADP By similarity UniProtKB P29475
Nucleotide binding1019 – 103416NADP By similarity UniProtKB P29475
Region387 – 41024Calmodulin-binding By similarity UniProtKB P29475
Region415 – 43420Tetrahydrobiopterin-binding By similarity UniProtKB P29475

Sites

Metal binding771Iron (heme axial ligand) By similarity UniProtKB P29475

Natural variations

Alternative sequence1 – 231231Missing in isoform 2.
VSP_040368

Experimental info

Sequence conflict6041D → G in BAH23564. Ref.2
Sequence conflict6751T → A in BAH23564. Ref.2
Sequence conflict8851Y → F in BAH23564. Ref.2
Sequence conflict9981T → M in BAH23564. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified April 29, 2008. Version 1.
Checksum: BE3639FFEA05DE35

FASTA1,097124,059
        10         20         30         40         50         60 
MAIPGRGDVP RDPEEVLNDA KDFLGQYFAS IRRANTPAHE ARWKIVQEEV ATTGTYQLTT 

        70         80         90        100        110        120 
TELVFGAKLA WRNASRCIGR IQWSKLQVFD CRQVTTTSGM FEALCNHIKY STNKGNIRSA 

       130        140        150        160        170        180 
ITLFPQRTDG KHDYRIWNSQ LISYAGYRQP DGTVLGDPMH CEFTDLCLKL GWKPPRTAWD 

       190        200        210        220        230        240 
ILPLVLSANG KDPDYFEIPR ELVMEIHMTH PTFEWFKELE LRWYALPAVS SMRFDCGGIE 

       250        260        270        280        290        300 
FTANAFNGWY MSTEIGCRNF CDTNRLNVLE KIAQNMGLDT RTPVNLWKDK ALVEVNVAVL 

       310        320        330        340        350        360 
HSFQQHNATI VDHHTASESF IKHLDNENRL RSGCPADWIW IVPPMSSSIT PVFHQEMALY 

       370        380        390        400        410        420 
YLKPSYEYQE PAWKTHQWQK SKPITGKRPI NRKFHFKQIA RAVKFTSKLF GRALSKRIKA 

       430        440        450        460        470        480 
TVLYATETGK SEQYAKELGV IFGHAFNAQV HCMADYDITS IEHEALLLVV TSTFGNGDPP 

       490        500        510        520        530        540 
ENGVAFGEHL CEILYADQVG EDSTGNQMLT PKSFIKANSD IQRYTAGNPK KLNRLESLKG 

       550        560        570        580        590        600 
STTDATSIDS FGPLSNVRFA VFALGSSAYP NFCNFGKYVD KLLVDLGGER IHDLATGDEM 

       610        620        630        640        650        660 
CGQDQAFRKW ASSVFNVACE TFCLDDDETL QEAKRALGTV ALSEETVQFA RAGCRPATLH 

       670        680        690        700        710        720 
AALQKSLNKQ FVSCTVKANK DLGDASAERS TIFIDLEPKE EIKYNPGDHV GIIACNRKEL 

       730        740        750        760        770        780 
VESLLSRIKD VDDYDEPLQL QLLKETHTSS GLVKSWEPHE KLPIMSVREL FTRFLDITTP 

       790        800        810        820        830        840 
PTTILLQYLA TTCEDEEEKK QLNVLATDPG AYEDWRHFHF PTLPEVLDQF PSARPNASLL 

       850        860        870        880        890        900 
AALLSPLQPR FYSISSSPLA HAKRLHLTVA VVTYRTQDGE GPVHYGVCSN YLMERKPGDE 

       910        920        930        940        950        960 
VYLFIRSAPN FHLPQDLSVP LILIGPGTGI APFRGFWHHR RALQNSCSRT TTGPVWLFFG 

       970        980        990       1000       1010       1020 
CRTKTMDLYR EEKEQALKEG VLSKVFLALS REKEVPKTYV QEVAENVGAE IHDLLINKGA 

      1030       1040       1050       1060       1070       1080 
HFYVCGDCKM AEDVHQKLKG IVKKHGNMTD EQVQNFMFML KEENRYHEDI FGITLRTAEV 

      1090 
HSASRESARR NRVASQP 

« Hide

Isoform 2 [UniParc].

Checksum: C37CF55A412E3D96
Show »

FASTA86697,550

References

[1]"Positional cloning of a Bombyx wingless locus flugellos (fl) reveals a crucial role for fringe that is specific for wing morphogenesis."
Sato K., Matsuoka-Matsunaga T., Futahashi R., Kojima T., Mita K., Banno Y., Fujiwara H.
Genetics 179:875-885(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
Tissue: Wing imaginal disk.
[2]"Identification of nitric-oxide synthase from Bombyx mori."
Nagaoka S., Takata Y.
Submitted (FEB-2009) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
Tissue: Reproductive system.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB360590 mRNA. Translation: BAG12563.1.
AB485776 mRNA. Translation: BAH23564.1.
RefSeqNP_001116808.1. NM_001123336.1.
UniGeneBmo.16070.

3D structure databases

ProteinModelPortalB1B557.
SMRB1B557. Positions 9-379, 417-493.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaBGIBMGA002937-RA; BGIBMGA002937-TA; BGIBMGA002937. [B1B557-1]
GeneID100144542.
KEGGbmor:100144542.

Organism-specific databases

CTD4843.

Phylogenomic databases

KOK13253.
OMAMALYYLK.
OrthoDBEOG79SDW7.

Family and domain databases

Gene3D1.20.990.10. 1 hit.
3.90.340.10. 1 hit.
InterProIPR003097. FAD-binding_1.
IPR017927. Fd_Rdtase_FAD-bd.
IPR001094. Flavdoxin.
IPR008254. Flavodoxin/NO_synth.
IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
IPR023173. NADPH_Cyt_P450_Rdtase_dom3.
IPR004030. NO_synthase_oxygenase_dom.
IPR012144. NOS_euk.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PANTHERPTHR19384:SF5. PTHR19384:SF5. 1 hit.
PfamPF00667. FAD_binding_1. 1 hit.
PF00258. Flavodoxin_1. 1 hit.
PF00175. NAD_binding_1. 1 hit.
PF02898. NO_synthase. 1 hit.
[Graphical view]
PIRSFPIRSF000333. NOS. 1 hit.
PRINTSPR00369. FLAVODOXIN.
PR00371. FPNCR.
SUPFAMSSF56512. SSF56512. 1 hit.
SSF63380. SSF63380. 1 hit.
PROSITEPS51384. FAD_FR. 1 hit.
PS50902. FLAVODOXIN_LIKE. 1 hit.
PS60001. NOS. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameNOSL_BOMMO
AccessionPrimary (citable) accession number: B1B557
Secondary accession number(s): B9X250
Entry history
Integrated into UniProtKB/Swiss-Prot: January 11, 2011
Last sequence update: April 29, 2008
Last modified: April 16, 2014
This is version 39 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

SIMILARITY comments

Index of protein domains and families