B1B557 (NOSL_BOMMO) Reviewed, UniProtKB/Swiss-Prot
Last modified
March 6, 2013.
Version 34.
History...
Clusters with 
Names·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: Nitric oxide synthase-like protein EC=1.14.13.39 | ||
| Gene names |
| ||
| Organism | Bombyx mori (Silk moth) [Reference proteome] | ||
| Taxonomic identifier | 7091 [NCBI] | ||
| Taxonomic lineage | Eukaryota › Metazoa › Ecdysozoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Lepidoptera › Glossata › Ditrysia › Bombycoidea › Bombycidae › Bombycinae › Bombyx |
Protein attributes
| Sequence length | 1097 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at transcript level |
General annotation (Comments)
| Function | Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body By similarity. |
| Catalytic activity | 2 L-arginine + 3 NADPH + 4 O2 = 2 L-citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O. |
| Cofactor | Heme group By similarity. UniProtKB P29475 Binds 1 FAD By similarity. UniProtKB P29475 Binds 1 FMN By similarity. UniProtKB P29475 |
| Sequence similarities | Belongs to the NOS family. Contains 1 FAD-binding FR-type domain. Contains 1 flavodoxin-like domain. |
Ontologies
| Keywords | |
|---|---|
| Coding sequence diversity | Alternative splicing |
| Ligand | Calmodulin-binding FAD FMN Heme Iron Metal-binding NADP |
| Molecular function | Oxidoreductase |
| Technical term | Complete proteome Reference proteome |
| Gene Ontology (GO) | |
| Biological_process | nitric oxide biosynthetic process Inferred from electronic annotation. Source: InterPro |
| Molecular_function | FMN binding Inferred from electronic annotation. Source: InterPro NADP bindingInferred from electronic annotation. Source: InterPro flavin adenine dinucleotide bindingInferred from electronic annotation. Source: InterPro heme bindingInferred from electronic annotation. Source: InterPro iron ion bindingInferred from electronic annotation. Source: InterPro nitric-oxide synthase activityInferred from electronic annotation. Source: EC |
| Complete GO annotation... | |
Alternative products
| This entry describes 2 isoforms produced by alternative splicing. [Align] [Select] Note: Additional isoforms seem to exist. | ||||||
| Isoform 1 (identifier: B1B557-1) This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. | ||||||
| Isoform 2 (identifier: B1B557-2) The sequence of this isoform differs from the canonical sequence as follows: 1-231: Missing. |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 1097 | 1097 | Nitric oxide synthase-like protein | PRO_0000403314 | |||||
Regions | |||||||||
| Domain | 420 – 615 | 196 | Flavodoxin-like | ||||||
| Domain | 669 – 914 | 246 | FAD-binding FR-type | ||||||
| Nucleotide binding | 426 – 430 | 5 | FMN By similarity | ||||||
| Nucleotide binding | 561 – 592 | 32 | FMN By similarity | ||||||
| Nucleotide binding | 704 – 715 | 12 | FAD By similarity UniProtKB P29475 | ||||||
| Nucleotide binding | 847 – 857 | 11 | FAD By similarity UniProtKB P29475 | ||||||
| Nucleotide binding | 922 – 940 | 19 | NADP By similarity UniProtKB P29475 | ||||||
| Nucleotide binding | 1019 – 1034 | 16 | NADP By similarity UniProtKB P29475 | ||||||
| Region | 387 – 410 | 24 | Calmodulin-binding By similarity UniProtKB P29475 | ||||||
| Region | 415 – 434 | 20 | Tetrahydrobiopterin-binding By similarity UniProtKB P29475 | ||||||
Sites | |||||||||
| Metal binding | 77 | 1 | Iron (heme axial ligand) By similarity UniProtKB P29475 | ||||||
Natural variations | |||||||||
| Alternative sequence | 1 – 231 | 231 | Missing in isoform 2. | VSP_040368 | |||||
Experimental info | |||||||||
| Sequence conflict | 604 | 1 | D → G in BAH23564. Ref.2 | ||||||
| Sequence conflict | 675 | 1 | T → A in BAH23564. Ref.2 | ||||||
| Sequence conflict | 885 | 1 | Y → F in BAH23564. Ref.2 | ||||||
| Sequence conflict | 998 | 1 | T → M in BAH23564. Ref.2 | ||||||
Sequences
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References
| [1] | "Positional cloning of a Bombyx wingless locus flugellos (fl) reveals a crucial role for fringe that is specific for wing morphogenesis." Sato K., Matsuoka-Matsunaga T., Futahashi R., Kojima T., Mita K., Banno Y., Fujiwara H. Genetics 179:875-885(2008) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Tissue: Wing imaginal disk. |
| [2] | "Identification of nitric-oxide synthase from Bombyx mori." Nagaoka S., Takata Y. Submitted (FEB-2009) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2). Tissue: Reproductive system. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | AB360590 mRNA. Translation: BAG12563.1. AB485776 mRNA. Translation: BAH23564.1. |
| RefSeq | NP_001116808.1. NM_001123336.1. |
| UniGene | Bmo.16070. |
3D structure databases | |
| ProteinModelPortal | B1B557. |
| SMR | B1B557. Positions 9-379, 417-493. |
| ModBase | Search... |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| EnsemblMetazoa | BGIBMGA002937-RA; BGIBMGA002937-TA; BGIBMGA002937. |
| GeneID | 100144542. |
Organism-specific databases | |
| CTD | 4843. |
Phylogenomic databases | |
| OMA | QVPCILI. |
| OrthoDB | EOG4CC2G5. |
Family and domain databases | |
| Gene3D | 1.20.990.10. 1 hit. 3.90.340.10. 1 hit. |
| InterPro | IPR003097. FAD-binding_1. IPR017927. Fd_Rdtase_FAD-bd. IPR001094. Flavdoxin. IPR008254. Flavodoxin/NO_synth. IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase. IPR023173. NADPH_Cyt_P450_Rdtase_dom3. IPR004030. NO_synthase_oxygenase_dom. IPR026009. NOS. IPR012144. NOS_met. IPR001433. OxRdtase_FAD/NAD-bd. IPR017938. Riboflavin_synthase-like_b-brl. [Graphical view] |
| PANTHER | PTHR19384:SF5. PTHR19384:SF5. 1 hit. |
| Pfam | PF00667. FAD_binding_1. 1 hit. PF00258. Flavodoxin_1. 1 hit. PF00175. NAD_binding_1. 1 hit. PF02898. NO_synthase. 1 hit. [Graphical view] |
| PIRSF | PIRSF000333. NOS. 1 hit. |
| PRINTS | PR00369. FLAVODOXIN. PR00371. FPNCR. |
| SUPFAM | SSF56512. NO_synthase_oxygenase_reg. 1 hit. SSF63380. Riboflavin_synthase_like_b-brl. 1 hit. |
| PROSITE | PS51384. FAD_FR. 1 hit. PS50902. FLAVODOXIN_LIKE. 1 hit. PS60001. NOS. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | NOSL_BOMMO | ||||||||
| Accession | Primary (citable) accession number: B1B557 Secondary accession number(s): B9X250 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
Relevant documents
| SIMILARITY comments Index of protein domains and families |