Disks large-associated protein 4 - Mus musculus (Mouse)

Preferred order of sections

Names and origin

Protein names

Recommended name:
Disks large-associated protein 4
Short name=DAP-4

Alternative name(s):
PSD-95/SAP90-binding protein 4
SAP90/PSD-95-associated protein 4
Short name=SAPAP-4

Gene names

Name:	Dlgap4
Synonyms:	Kiaa0964, Sapap4

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	992 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May play a role in the molecular organization of synapses and neuronal cell signaling. Could be an adapter protein linking ion channel to the subsynaptic cytoskeleton. May induce enrichment of PSD-95/SAP90 at the plasma membrane By similarity.
Subunit structure	Interacts with DLG1 and DLG4/PSD-95 By similarity.
Subcellular location	Membrane; Peripheral membrane protein By similarity.
Sequence similarities	Belongs to the SAPAP family.
Sequence caution	The sequence BAC65690.2 differs from that shown. Reason: Intron retention.

Ontologies

Keywords
Cellular component	Membrane
Coding sequence diversity	Alternative splicing
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cell-cell signaling Inferred from electronic annotation. Source: InterPro
Cellular component	synapse Inferred from direct assay Ref.1. Source: MGI
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: B1AZP2-1) This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: B1AZP2-2) The sequence of this isoform differs from the canonical sequence as follows: 671-700: VDCIQPVPKEEPSPATKFQSIGIQVEDDWR → ERTRRSGSHLSEDNGPKAIDVMAPSSE

Isoform 3 (identifier: B1AZP2-3) The sequence of this isoform differs from the canonical sequence as follows: 1-539: Missing. 540-550: GSLSNSRTLPS → MALCLELLKQC

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 992

992

Disks large-associated protein 4

PRO_0000345018

Amino acid modifications

Modified residue

367

1

Phosphoserine Ref.6

Modified residue

368

1

Phosphotyrosine By similarity

Modified residue

377

1

Phosphoserine Ref.6

Modified residue

397

1

Phosphoserine Ref.6

Modified residue

421

1

Phosphoserine Ref.7

Modified residue

512

1

Phosphoserine Ref.6

Modified residue

665

1

Phosphoserine Ref.8

Modified residue

915

1

Phosphothreonine Ref.7

Modified residue

973

1

Phosphoserine Ref.5

Modified residue

984

1

Phosphotyrosine By similarity

Natural variations

Alternative sequence

1 – 539

539

Missing in isoform 3.

VSP_034907

Alternative sequence

540 – 550

11

GSLSNSRTLPS → MALCLELLKQC in isoform 3.

VSP_034908

Alternative sequence

671 – 700

30

VDCIQ…EDDWR → ERTRRSGSHLSEDNGPKAID VMAPSSE in isoform 2.

VSP_034909

Experimental info

Sequence conflict

614

1

S → N in AAH24558. Ref.4

Sequence conflict

676

1

Missing in AAO89220. Ref.1

Sequence conflict

689

1

Q → E in AAO89220. Ref.1

Sequence conflict

693

1

I → V in AAO89220. Ref.1

Sequence conflict

693

1

I → V in AAH24558. Ref.4

Sequence conflict

693

1

I → V in AAI06095. Ref.4

Sequence conflict

774

1

A → V in AAH24558. Ref.4

Sequences

Sequence		Length	Mass (Da)
Isoform 1 [UniParc]. Last modified April 8, 2008. Version 1. Checksum: A33016920922ABB9 Show »	FASTA	992	108,037
10 20 30 40 50 60 MKGLGDSRPR HLSDSLDPPH EPLFAGPDRN PYLLSPTEAF AREARFPGQN TLPGDGLFPL 70 80 90 100 110 120 NNQLPPPSST FPRIHYNSHF EVPEESPFPS HAQATKINRL PANLLDQFEK QLPIHRDGFS 130 140 150 160 170 180 TLQFPRGEAK ARGESPGRIR HLVHSVQRLF FTKAPSMEGT AGKVGGNGSK KGGLEDGKGR 190 200 210 220 230 240 RAKSKERAKA GEPKRRSRSN ISGWWSSDDN LDGEGGAFRS GPASGLMTLG RQQERTQPRY 250 260 270 280 290 300 FMHAYNTISG HMLKTTKNTT TELTAPPPPP APPATCPSLG VGTDTNYVKR GSWSTLTLSH 310 320 330 340 350 360 AHEVCQKTSA TLDKSLLKSK SCHQGLAYHY LQVPGGGGEW STTLLSPRDM DSTAEGPIPC 370 380 390 400 410 420 RRMRSGSYIK AMGDEDSDES GGGSPKPSPK TAARRQSYLR ATQQSLGEQS NPRRSLDRLD 430 440 450 460 470 480 SVDMLLPSKC PSWEDDYNPI SDSLNDSSCI SQVFGQASLI PQLFGHDQQV READLSDQYE 490 500 510 520 530 540 AACESACSEA ESTTAEALDL PLPSYFRSRS HSYLRAIQAG CSQEEDSVSL QSLSPPPSTG 550 560 570 580 590 600 SLSNSRTLPS SSCLVAYKKT PPPVPPRTTS KPFISVTVQS STESAQDTYL DSQDHKSEVT 610 620 630 640 650 660 SQSGLSNSSD SLDSSTRPPS VTRGGITPGP EAPEPPPKHA ALKSEQGTLT SSESHSEAIP 670 680 690 700 710 720 KRKLSSIGIQ VDCIQPVPKE EPSPATKFQS IGIQVEDDWR SSAPSHSMSS RRDTDSDTQD 730 740 750 760 770 780 ANDSSCKSSE RSLPDCTSHP NSISIDAGPR QAPKIAQIKR NLSYGDNSDP ALEASSLPPP 790 800 810 820 830 840 DPWLETSSSS PAEPAQPGAC RRDGYWFLKL LQAETERLEG WCCQMDKETK ENNLSEEVLG 850 860 870 880 890 900 KVLSAVGSAQ LLMSQKFQQF RGLCEQNLNP DANPRPTAQD LAGFWDLLQL SIEDISMKFD 910 920 930 940 950 960 ELYHLKANSW QLVETPEKRK EEKKPPPPVP KKPAKSKAAV SRDKASDAGD KQRQEARKRL 970 980 990 LAAKRAASVR QNSATESADS IEIYVPEAQT RL « Hide
Isoform 2 [UniParc]. Checksum: FC0D22EB3BD0C09F Show »	FASTA	989	107,551
10 20 30 40 50 60 MKGLGDSRPR HLSDSLDPPH EPLFAGPDRN PYLLSPTEAF AREARFPGQN TLPGDGLFPL 70 80 90 100 110 120 NNQLPPPSST FPRIHYNSHF EVPEESPFPS HAQATKINRL PANLLDQFEK QLPIHRDGFS 130 140 150 160 170 180 TLQFPRGEAK ARGESPGRIR HLVHSVQRLF FTKAPSMEGT AGKVGGNGSK KGGLEDGKGR 190 200 210 220 230 240 RAKSKERAKA GEPKRRSRSN ISGWWSSDDN LDGEGGAFRS GPASGLMTLG RQQERTQPRY 250 260 270 280 290 300 FMHAYNTISG HMLKTTKNTT TELTAPPPPP APPATCPSLG VGTDTNYVKR GSWSTLTLSH 310 320 330 340 350 360 AHEVCQKTSA TLDKSLLKSK SCHQGLAYHY LQVPGGGGEW STTLLSPRDM DSTAEGPIPC 370 380 390 400 410 420 RRMRSGSYIK AMGDEDSDES GGGSPKPSPK TAARRQSYLR ATQQSLGEQS NPRRSLDRLD 430 440 450 460 470 480 SVDMLLPSKC PSWEDDYNPI SDSLNDSSCI SQVFGQASLI PQLFGHDQQV READLSDQYE 490 500 510 520 530 540 AACESACSEA ESTTAEALDL PLPSYFRSRS HSYLRAIQAG CSQEEDSVSL QSLSPPPSTG 550 560 570 580 590 600 SLSNSRTLPS SSCLVAYKKT PPPVPPRTTS KPFISVTVQS STESAQDTYL DSQDHKSEVT 610 620 630 640 650 660 SQSGLSNSSD SLDSSTRPPS VTRGGITPGP EAPEPPPKHA ALKSEQGTLT SSESHSEAIP 670 680 690 700 710 720 KRKLSSIGIQ ERTRRSGSHL SEDNGPKAID VMAPSSESSA PSHSMSSRRD TDSDTQDAND 730 740 750 760 770 780 SSCKSSERSL PDCTSHPNSI SIDAGPRQAP KIAQIKRNLS YGDNSDPALE ASSLPPPDPW 790 800 810 820 830 840 LETSSSSPAE PAQPGACRRD GYWFLKLLQA ETERLEGWCC QMDKETKENN LSEEVLGKVL 850 860 870 880 890 900 SAVGSAQLLM SQKFQQFRGL CEQNLNPDAN PRPTAQDLAG FWDLLQLSIE DISMKFDELY 910 920 930 940 950 960 HLKANSWQLV ETPEKRKEEK KPPPPVPKKP AKSKAAVSRD KASDAGDKQR QEARKRLLAA 970 980 KRAASVRQNS ATESADSIEI YVPEAQTRL « Hide
Isoform 3 [UniParc]. Checksum: F631D60B5FB7D916 Show »	FASTA	453	49,460
10 20 30 40 50 60 MALCLELLKQ CSSCLVAYKK TPPPVPPRTT SKPFISVTVQ SSTESAQDTY LDSQDHKSEV 70 80 90 100 110 120 TSQSGLSNSS DSLDSSTRPP SVTRGGITPG PEAPEPPPKH AALKSEQGTL TSSESHSEAI 130 140 150 160 170 180 PKRKLSSIGI QVDCIQPVPK EEPSPATKFQ SIGIQVEDDW RSSAPSHSMS SRRDTDSDTQ 190 200 210 220 230 240 DANDSSCKSS ERSLPDCTSH PNSISIDAGP RQAPKIAQIK RNLSYGDNSD PALEASSLPP 250 260 270 280 290 300 PDPWLETSSS SPAEPAQPGA CRRDGYWFLK LLQAETERLE GWCCQMDKET KENNLSEEVL 310 320 330 340 350 360 GKVLSAVGSA QLLMSQKFQQ FRGLCEQNLN PDANPRPTAQ DLAGFWDLLQ LSIEDISMKF 370 380 390 400 410 420 DELYHLKANS WQLVETPEKR KEEKKPPPPV PKKPAKSKAA VSRDKASDAG DKQRQEARKR 430 440 450 LLAAKRAASV RQNSATESAD SIEIYVPEAQ TRL « Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Differential mRNA expression and protein localization of the SAP90/PSD-95-associated proteins (SAPAPs) in the nervous system of the mouse." Welch J.M., Wang D., Feng G. J. Comp. Neurol. 472:24-39(2004) [PubMed: 15024750] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Strain: ICR.
[2]	"Prediction of the coding sequences of mouse homologues of KIAA gene: II. The complete nucleotide sequences of 400 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries." Okazaki N., Kikuno R., Ohara R., Inamoto S., Aizawa H., Yuasa S., Nakajima D., Nagase T., Ohara O., Koga H. DNA Res. 10:35-48(2003) [PubMed: 12693553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Brain.
[3]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed: 19468303] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6J.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 3). Strain: C57BL/6, FVB/N and FVB/N-3. Tissue: Brain and Mammary tumor.
[5]	"Phosphoproteomic analysis of the developing mouse brain." Ballif B.A., Villen J., Beausoleil S.A., Schwartz D., Gygi S.P. Mol. Cell. Proteomics 3:1093-1101(2004) [PubMed: 15345747] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-973, MASS SPECTROMETRY. Tissue: Embryonic brain.
[6]	"Comprehensive identification of phosphorylation sites in postsynaptic density preparations." Trinidad J.C., Specht C.G., Thalhammer A., Schoepfer R., Burlingame A.L. Mol. Cell. Proteomics 5:914-922(2006) [PubMed: 16452087] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-367; SER-377; SER-397 AND SER-512, MASS SPECTROMETRY. Tissue: Brain.
[7]	"Qualitative and quantitative analyses of protein phosphorylation in naive and stimulated mouse synaptosomal preparations." Munton R.P., Tweedie-Cullen R., Livingstone-Zatchej M., Weinandy F., Waidelich M., Longo D., Gehrig P., Potthast F., Rutishauser D., Gerrits B., Panse C., Schlapbach R., Mansuy I.M. Mol. Cell. Proteomics 6:283-293(2007) [PubMed: 17114649] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-421 AND THR-915, MASS SPECTROMETRY. Tissue: Brain cortex.
[8]	"Solid tumor proteome and phosphoproteome analysis by high resolution mass spectrometry." Zanivan S., Gnad F., Wickstroem S.A., Geiger T., Macek B., Cox J., Faessler R., Mann M. J. Proteome Res. 7:5314-5326(2008) [PubMed: 19367708] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-665, MASS SPECTROMETRY. Tissue: Melanoma.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK122408 Transcribed RNA. Translation: BAC65690.2. Sequence problems. AY243849 mRNA. Translation: AAO89220.2. BX004793, BX571766 Genomic DNA. Translation: CAM16917.1. BX571766, BX004793 Genomic DNA. Translation: CAM22345.1. BX004793, BX571766 Genomic DNA. Translation: CAM16918.1. BX571766, BX004793 Genomic DNA. Translation: CAM22346.1. BX004793, BX571766 Genomic DNA. Translation: CAM16919.1. BX571766, BX004793 Genomic DNA. Translation: CAM22347.1. BC024558 mRNA. Translation: AAH24558.1. BC058948 mRNA. Translation: AAH58948.1. BC085475 mRNA. Translation: AAH85475.1. BC106094 mRNA. Translation: AAI06095.1. BC141110 mRNA. Translation: AAI41111.1. BC145394 mRNA. Translation: AAI45395.1.
IPI	IPI00330186. IPI00377469. IPI00752220.
RefSeq	NP_001035952.1. NM_001042487.1. NP_001035953.1. NM_001042488.1. NP_666240.4. NM_146128.5.
UniGene	Mm.22094.
3D structure databases
ProteinModelPortal	B1AZP2.
ModBase	Search...
Protein-protein interaction databases
IntAct	B1AZP2. 2 interactions.
STRING	B1AZP2.
PTM databases
PhosphoSite	B1AZP2.
Proteomic databases
PRIDE	B1AZP2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000000094; ENSMUSP00000000094; ENSMUSG00000061689. ENSMUST00000070782; ENSMUSP00000068745; ENSMUSG00000061689. ENSMUST00000099145; ENSMUSP00000096749; ENSMUSG00000061689. ENSMUST00000109567; ENSMUSP00000105195; ENSMUSG00000061689. ENSMUST00000169464; ENSMUSP00000126980; ENSMUSG00000061689.
GeneID	228836.
KEGG	mmu:228836.
UCSC	uc008nnr.1. mouse. uc008nnt.1. mouse. uc012chv.1. mouse.
Organism-specific databases
CTD	22839.
MGI	MGI:2138865. Dlgap4.
Rouge	Search...
Phylogenomic databases
GeneTree	ENSGT00550000074473.
HOVERGEN	HBG018957.
InParanoid	B1AZP2.
OMA	EGPIPCR.
OrthoDB	EOG4ZCT3Z.
PhylomeDB	B1AZP2.
Gene expression databases
Bgee	B1AZP2.
Genevestigator	B1AZP2.
Family and domain databases
InterPro	IPR005026. GKAP. [Graphical view]
Pfam	PF03359. GKAP. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	379186.
SOURCE	Search...

Entry information

Entry name

DLGP4_MOUSE

Accession

Primary (citable) accession number: B1AZP2
Secondary accession number(s): B1AZP3

Q8R3U9

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 22, 2008
Last sequence update:	April 8, 2008
Last modified:	October 19, 2011
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Alternative products

Sequence annotation (Features)

Molecule processing

Amino acid modifications

Natural variations

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents