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Protein

THO complex subunit 2

Gene

Thoc2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for efficient export of polyadenylated RNA and spliced mRNA. Acts as component of the THO subcomplex of the TREX complex which is thought to couple mRNA transcription, processing and nuclear export, and which specifically associates with spliced mRNA and not with unspliced pre-mRNA. TREX is recruited to spliced mRNAs by a transcription-independent mechanism, binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing- and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export to the cytoplasm via the TAP/NFX1 pathway. Plays a role for proper neuronal development.By similarity

GO - Molecular functioni

  • mRNA binding Source: MGI

GO - Biological processi

  • blastocyst development Source: MGI
  • cell morphogenesis Source: MGI
  • generation of neurons Source: UniProtKB
  • mRNA export from nucleus Source: MGI
  • mRNA processing Source: UniProtKB-KW
  • negative regulation of neuron projection development Source: Ensembl
  • neuron development Source: UniProtKB
  • poly(A)+ mRNA export from nucleus Source: MGI
  • regulation of gene expression Source: MGI
  • regulation of mRNA export from nucleus Source: MGI
  • RNA splicing Source: UniProtKB-KW
  • stem cell division Source: MGI
  • viral mRNA export from host cell nucleus Source: MGI
Complete GO annotation...

Keywords - Biological processi

mRNA processing, mRNA splicing, mRNA transport, Transport

Keywords - Ligandi

RNA-binding

Enzyme and pathway databases

ReactomeiR-MMU-109688. Cleavage of Growing Transcript in the Termination Region.
R-MMU-159236. Transport of Mature mRNA derived from an Intron-Containing Transcript.
R-MMU-72187. mRNA 3'-end processing.

Names & Taxonomyi

Protein namesi
Recommended name:
THO complex subunit 2
Short name:
Tho2
Gene namesi
Name:Thoc2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome X

Organism-specific databases

MGIiMGI:2442413. Thoc2.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003843991 – 1594THO complex subunit 2Add BLAST1594

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1222PhosphoserineCombined sources1
Modified residuei1385PhosphothreonineBy similarity1
Modified residuei1390PhosphoserineBy similarity1
Modified residuei1393PhosphoserineCombined sources1
Modified residuei1417PhosphoserineCombined sources1
Modified residuei1450PhosphoserineBy similarity1
Modified residuei1486PhosphoserineBy similarity1
Modified residuei1516PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiB1AZI6.
MaxQBiB1AZI6.
PaxDbiB1AZI6.
PeptideAtlasiB1AZI6.
PRIDEiB1AZI6.

PTM databases

iPTMnetiB1AZI6.
PhosphoSitePlusiB1AZI6.

Expressioni

Tissue specificityi

Expressed in the hippocampus and the cortical neurons.1 Publication

Gene expression databases

BgeeiENSMUSG00000037475.
ExpressionAtlasiB1AZI6. baseline and differential.
GenevisibleiB1AZI6. MM.

Interactioni

Subunit structurei

Component of the THO complex, which is composed of THOC1, THOC2, THOC3, THOC5, THOC6 and THOC7; together with at least ALYREF/THOC4, DDX39B, SARNP/CIP29 and CHTOP, THO forms the transcription/export (TREX) complex which seems to have a dynamic structure involving ATP-dependent remodeling. Interacts with THOC1, POLDIP3 and ZC3H11A (By similarity).By similarity

Protein-protein interaction databases

BioGridi237091. 1 interactor.
STRINGi10090.ENSMUSP00000044677.

Structurei

3D structure databases

ProteinModelPortaliB1AZI6.
SMRiB1AZI6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili896 – 965Sequence analysisAdd BLAST70
Coiled coili1464 – 1491Sequence analysisAdd BLAST28

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi923 – 928Nuclear localization signalSequence analysis6

Sequence similaritiesi

Belongs to the THOC2 family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG1874. Eukaryota.
ENOG410XQ2A. LUCA.
GeneTreeiENSGT00710000106792.
HOGENOMiHOG000048227.
InParanoidiB1AZI6.
KOiK12879.
OMAiIYELLWQ.
OrthoDBiEOG091G00GM.
PhylomeDBiB1AZI6.
TreeFamiTF313127.

Family and domain databases

InterProiIPR021418. THO_THOC2_C.
IPR021726. THO_THOC2_N.
IPR032302. THOC2_N.
[Graphical view]
PfamiPF11262. Tho2. 1 hit.
PF11732. Thoc2. 1 hit.
PF16134. THOC2_N. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B1AZI6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAAAVVVPA EWIKNWEKSG RGEFLHLCRI LSENKSHDSS TYRDFQQALY
60 70 80 90 100
ELSYHVIKGN LKHEQASSVL NDISEFREDM PSILADVFCI LDIETNCLEE
110 120 130 140 150
KSKRDYFTQL VLACLYLVSD TVLKERLDPE TLESLGLIKQ SQQFNQKSVK
160 170 180 190 200
IKTKLFYKQQ KFNLLREENE GYAKLIAELG QDLSGNITSD LILENIKSLI
210 220 230 240 250
GCFNLDPNRV LDVILEVFEC RPEHDDFFIS LLESYMSMCE PQTLCHILGF
260 270 280 290 300
KFKFYQEPSG ETPSSLYRVA AVLLQFNLID LDDLYVHLLP ADNCIMDEYK
310 320 330 340 350
REIVEAKQIV RKLTMVVLSS EKLDERDKEK DKDDEKVEKP PDNQKLGLLE
360 370 380 390 400
ALLKVGDWQH AQNIMDQMPP YYAASHKLIA LAICKLIHIT VEPLYRRVGV
410 420 430 440 450
PKGAKGSPVS ALQNKRAPKQ VESFEDLRRD VFNMFCYLGP HLSHDPILFA
460 470 480 490 500
KVVRIGKSFM KEFQSDGSKQ EDKEKTEVIL SCLLSITDQV LLPSLSLMDC
510 520 530 540 550
NACMSEELWG MFKTFPYQHR YRLYGQWKNE TYNGHPLLVK VKAQTIDRAK
560 570 580 590 600
YIMKRLTKEN VKPSGRQIGK LSHSNPTILF DYILSQIQKY DNLITPVVDS
610 620 630 640 650
LKYLTSLNYD VLAYCIIEAL ANPEKERMKH DDTTISSWLQ SLASFCGAVF
660 670 680 690 700
RKYPIDLAGL LQYVANQLKA GKSFDLLILK EVVQKMAGIE ITEEMTMEQL
710 720 730 740 750
EAMTGGEQLK AEGGYFGQIR NTKKSSQRLK DALLDHDLAL PLCLLMAQQR
760 770 780 790 800
NGVIFQEGGE KHLKLVGKLY DQCHDTLVQF GGFLASNLST EDYIKRVPSI
810 820 830 840 850
DVLCNEFHTP HDAAFFLSRP MYAHHISSKY DELKKSEKGS KQQHKVHKYI
860 870 880 890 900
TSCEMVMAPV HEAVVSLHVS KVWDDISPQF YATFWSLTMY DLAVPHTSYE
910 920 930 940 950
REVNKLKVQM KAIDDNQEMP PNKKKKEKER CTALQDKLLE EEKKQMEHVQ
960 970 980 990 1000
RVLQRLKLEK DNWLLAKSTK NETITKFLQL CIFPRCIFSA IDAVYCARFV
1010 1020 1030 1040 1050
ELVHQQKTPN FSTLLCYDRV FSDIIYTVAS CTENEASRYG RFLCCMLETV
1060 1070 1080 1090 1100
TRWHSDRATY EKECGNYPGF LTILRATGFD GGNKADQLDY ENFRHVVHKW
1110 1120 1130 1140 1150
HYKLTKASVH CLETGEYTHI RNILIVLTKI LPWYPKVLNL GQALERRVNK
1160 1170 1180 1190 1200
ICQEEKEKRP DLYALAMGYS GQLKSRKSHM IPENEFHHKD PPPRNAVASV
1210 1220 1230 1240 1250
QNGPGGGTSS SSIGNASKSD ESGAEETDKS RERSQCGTKA VNKASSTTPK
1260 1270 1280 1290 1300
GNSSNGNSGS NSNKAVKEND KEKVKEKEKE KKEKTPATTP EARALGKDSK
1310 1320 1330 1340 1350
EKPKEERPNK EDKARETKER TPKSDKEKEK FKKEEKAKDE KFKTTVPIVE
1360 1370 1380 1390 1400
SKSTQERERE KEPSRERDVA KEMKSKENVK GGEKTPVSGS LKSPVPRSDI
1410 1420 1430 1440 1450
SEPDREQKRR KIDSHPSPSH SSTVKDSLID LKDSSAKLYI NHNPPPLSKS
1460 1470 1480 1490 1500
KEREMDKKDL DKSRERSRER EKKDEKDRKE RKRDHSNNDR EVPPDITKRR
1510 1520 1530 1540 1550
KEENGTMGVS KHKSESPCES QYPNEKDKEK NKSKSSGKEK SSSDSFKSEK
1560 1570 1580 1590
MDKISSGGKK ESRHDKEKIE KKEKRDSSGG KEEKKHHKSS DKHR
Length:1,594
Mass (Da):182,773
Last modified:April 8, 2008 - v1
Checksum:i9732F53F9C506CC8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX005253, AL954355 Genomic DNA. Translation: CAM20174.1.
AL954355, BX005253 Genomic DNA. Translation: CAM27369.1.
CCDSiCCDS40951.1.
RefSeqiNP_001028594.1. NM_001033422.1.
UniGeneiMm.259498.

Genome annotation databases

EnsembliENSMUST00000047037; ENSMUSP00000044677; ENSMUSG00000037475.
GeneIDi331401.
KEGGimmu:331401.
UCSCiuc009tao.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX005253, AL954355 Genomic DNA. Translation: CAM20174.1.
AL954355, BX005253 Genomic DNA. Translation: CAM27369.1.
CCDSiCCDS40951.1.
RefSeqiNP_001028594.1. NM_001033422.1.
UniGeneiMm.259498.

3D structure databases

ProteinModelPortaliB1AZI6.
SMRiB1AZI6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi237091. 1 interactor.
STRINGi10090.ENSMUSP00000044677.

PTM databases

iPTMnetiB1AZI6.
PhosphoSitePlusiB1AZI6.

Proteomic databases

EPDiB1AZI6.
MaxQBiB1AZI6.
PaxDbiB1AZI6.
PeptideAtlasiB1AZI6.
PRIDEiB1AZI6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000047037; ENSMUSP00000044677; ENSMUSG00000037475.
GeneIDi331401.
KEGGimmu:331401.
UCSCiuc009tao.1. mouse.

Organism-specific databases

CTDi57187.
MGIiMGI:2442413. Thoc2.

Phylogenomic databases

eggNOGiKOG1874. Eukaryota.
ENOG410XQ2A. LUCA.
GeneTreeiENSGT00710000106792.
HOGENOMiHOG000048227.
InParanoidiB1AZI6.
KOiK12879.
OMAiIYELLWQ.
OrthoDBiEOG091G00GM.
PhylomeDBiB1AZI6.
TreeFamiTF313127.

Enzyme and pathway databases

ReactomeiR-MMU-109688. Cleavage of Growing Transcript in the Termination Region.
R-MMU-159236. Transport of Mature mRNA derived from an Intron-Containing Transcript.
R-MMU-72187. mRNA 3'-end processing.

Miscellaneous databases

ChiTaRSiThoc2. mouse.
PROiB1AZI6.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000037475.
ExpressionAtlasiB1AZI6. baseline and differential.
GenevisibleiB1AZI6. MM.

Family and domain databases

InterProiIPR021418. THO_THOC2_C.
IPR021726. THO_THOC2_N.
IPR032302. THOC2_N.
[Graphical view]
PfamiPF11262. Tho2. 1 hit.
PF11732. Thoc2. 1 hit.
PF16134. THOC2_N. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTHOC2_MOUSE
AccessioniPrimary (citable) accession number: B1AZI6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: April 8, 2008
Last modified: November 2, 2016
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.