B1AY13 (UBP24_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ubiquitin carboxyl-terminal hydrolase 24
EC=3.4.19.12

Alternative name(s):
Deubiquitinating enzyme 24
Ubiquitin thioesterase 24
Ubiquitin-specific-processing protease 24

Gene names

Name:

Usp24

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	2617 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in the ubiquitin-dependent proteolytic pathway in conjunction with the 26S proteasome By similarity.
Catalytic activity	Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
Sequence similarities	Belongs to the peptidase C19 family. Contains 1 UBA domain.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Coding sequence diversity	Alternative splicing
Molecular function	Hydrolase Protease Thiol protease
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	ubiquitin-dependent protein catabolic process Inferred from electronic annotation. Source: InterPro
Molecular_function	cysteine-type peptidase activity Inferred from electronic annotation. Source: UniProtKB-KW ubiquitin thiolesterase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: B1AY13-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: B1AY13-2)

The sequence of this isoform differs from the canonical sequence as follows:
     408-418: TKLIEDSTLSK → SVHLSHMWPLM
     419-2617: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 2617	2617	Ubiquitin carboxyl-terminal hydrolase 24		PRO_0000353213
Regions
	Domain	3 – 44	42	UBA
	Compositional bias	47 – 91	45	Gly-rich
	Compositional bias	1032 – 1059	28	Ser-rich
Sites
	Active site	1695	1	Nucleophile By similarity
	Active site	1967	1	Proton acceptor By similarity
Amino acid modifications
	Modified residue	62	1	Phosphoserine By similarity
	Modified residue	1138	1	Phosphoserine By similarity
	Modified residue	2044	1	Phosphoserine Ref.4
	Modified residue	2556	1	Phosphothreonine Ref.4
	Modified residue	2558	1	Phosphoserine By similarity
	Modified residue	2562	1	Phosphothreonine By similarity
	Modified residue	2601	1	Phosphoserine By similarity
Natural variations
	Alternative sequence	408 – 418	11	TKLIEDSTLSK → SVHLSHMWPLM in isoform 2.		VSP_035661
	Alternative sequence	419 – 2617	2199	Missing in isoform 2.		VSP_035662

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified April 8, 2008. Version 1. Checksum: 802DFE22143B2C94	FASTA	2,617	294,001
	10 20 30 40 50 60 MESEEEQHMT TLLCMGFSDP ATIRKALRLA KNDINEAVAL LTNERPGLDY GGYEPMDSGG 70 80 90 100 110 120 PSPGPGGGPR GDSGSDGSGP SRGGSTGGGG GFDPPPAYHE VVDAEKNDEN GNCSGEGIEF 130 140 150 160 170 180 PTTNLYELES RVLTDHWSIP YKREESLGKC LLASTYLARL GLSESDENCK RFMERCMPEA 190 200 210 220 230 240 FKKLLTSSAV HKWGTEIHEG IYNMLMLLIE LVAERMKQDP IPIGLLGVLT MAFNPDNEYH 250 260 270 280 290 300 FKNRMKVSQR NWAEVFGEGN MFAISPVSTF QKEPHGWVVD LVNKFGELGG FAAIQAKLHS 310 320 330 340 350 360 EDIELGAVSA LVQPLGVCAE YLNSSVVQPM LDPVILTTIQ DVRSVEEKDL KDKRLVSIPE 370 380 390 400 410 420 LLSAIKLLCM RFQPALVTTV DALRLDILLR MLKSPHFSAK MNSLKEVTKL IEDSTLSKSV 430 440 450 460 470 480 KNAIDTDRLL DWLVENSVLS IALEGNIDQA QYCDRIKGII ELLGSKLSLD ELTKIWKIQS 490 500 510 520 530 540 GQSSTVIENI HTIIAAAAVK FNADQLNHLF VLIQKSWETE SDRVRQKLLS LIGRIGREAR 550 560 570 580 590 600 FEATSGKVLD VLWELAHLPT LPSSLIQQAL EEHLTILSDA YAVKEAVKRS YIIKCIEDIK 610 620 630 640 650 660 RPGEWSSLEK NKKDGFKSSQ LNNPQFVWVV PALRQLHEIT RSFIKQTYQK QDKSIIQDLK 670 680 690 700 710 720 KNFEIVKLVT GSLLACHRLA AAVAGPGGLT GLTLVDGRYT YREYLEAHLK FLAFFLQEAT 730 740 750 760 770 780 LYLGWNRAKE IWECLVTGQD VCELDREMCF EWFTKGQHDL ESDVQQQLFK EKILKLESYE 790 800 810 820 830 840 ITMNGFNLFK TFFENVNLCD HRLKRQGAQL YVEKLELVGM DFIWKIAMES PDEEIANEAI 850 860 870 880 890 900 QLIINYSYIN LNPRLKKDSV SLHKKFIADC YTRLEAASSA LGGPTLTHAV TRATKMLTAT 910 920 930 940 950 960 AMPTVATSVQ SPYRSTKLVI IERLLLLAER YVITIEDFYS VPRTILPHGA SFHGHLLTLN 970 980 990 1000 1010 1020 VTYESTKDTF TVEAHSNETI GSVRWKIAKQ LCSPVDNIQI FTNDSLLTVN KDQKLLHQLG 1030 1040 1050 1060 1070 1080 FSDEQVLTVK TSGSGTPSGS SADSSTSSSS SSSGAFSSSY AMEQEKSLPG VVMALVCNVF 1090 1100 1110 1120 1130 1140 DMLYQLANLE EPRITLRVRK LLLLIPTDPA IQEALDQLDS LGRKKTLLSE TSSQSSKSPS 1150 1160 1170 1180 1190 1200 LSSKQQHQPS ASSILESLFR SFAPGMSTFR VLYNLEVLSS KLMPTADDDM ARSCAKSFCE 1210 1220 1230 1240 1250 1260 NFLKAGGLSL VVNVMQRDSI PSEVDYETRQ GVYSICLQLA RFLLVGQTMP TSLDEDLTKD 1270 1280 1290 1300 1310 1320 GIEALSSRPF RNVSRQTSRQ MSLCGTPEKS SYRQLSVSDR SSIRVEEIIP AARVAIQTME 1330 1340 1350 1360 1370 1380 ASDFTATVAC FMRLSWAAAA GRLDLVGSSQ PIKESNSLFP AGIRSRLSSS GSNCSSSSEG 1390 1400 1410 1420 1430 1440 EPAALHAGIC VRQQSVSTKD ALIAGEALSL LVTCLQLRSQ QLASFYSLPC VADFIIDILL 1450 1460 1470 1480 1490 1500 GSPSAEIRRV ACDQLYTLSQ TDTSAHPEVQ KPNQFLLGVI LTAQLPLWSP TSIMRGVNQR 1510 1520 1530 1540 1550 1560 LLSQCMEYFD LRCQLLDDLT TSEMDQLRIS PATMLEDEIT WLDNFEPNRT ADCETSEADN 1570 1580 1590 1600 1610 1620 ILLAGHLRLI KTLLSLCGAE KEMLGSSLIK PLLDDFLFRA SRIIVNSHSP ASSAAISQQD 1630 1640 1650 1660 1670 1680 FHPKCSTVNS RLAAYEVLVM LADSSPSNLQ IITKELLSMH HQPDPALTKE FDYLPPVDSR 1690 1700 1710 1720 1730 1740 SSSGFVGLRN GGATCYMNAV FQQLYMQPGL PESLLSVDDD TDNPDDSVFY QVQSLFGHLM 1750 1760 1770 1780 1790 1800 ESKLQYYVPE NFWKIFKMWN KELYVREQQD AYEFFTSLID QMDEYLKKMG REQIFKNTFQ 1810 1820 1830 1840 1850 1860 GIYSDQKICK DCPHRYEREE AFMALNLGVT SCQSLEISLD QFVRGEVLEG SNAYYCEKCK 1870 1880 1890 1900 1910 1920 EKRITVKRTC IKSLPSVLVI HLMRFGFDWE SGRSIKYDEQ IRFPWMLNME PYTVAGMARQ 1930 1940 1950 1960 1970 1980 DSSSEVGENG RNMDQGGGGS PRKKVALTEN YELVGVIVHS GQAHAGHYYS FIKDRRGCGK 1990 2000 2010 2020 2030 2040 GKWYKFNDTV IEEFDLNDET LEYECFGGEY RPKVYDQTNP YTDVRRRYWN AYMLFYQRVS 2050 2060 2070 2080 2090 2100 DQNSPVLPKK SRVSVVRQEA EDLSLSAPSS PEISPQSSPR PHRPNNDRLS ILTKLVKKGE 2110 2120 2130 2140 2150 2160 KKGLFVEKMP ARIYQMVRDE NLKFMKNRDV YSSDYFSFVL SLASLNATKL KHPYYPCMAK 2170 2180 2190 2200 2210 2220 VSLQLAIQFL FQTYLRTKKK LRVDTEEWIA TIEALLSKSL DACQWLVEYF ISSEGRELVK 2230 2240 2250 2260 2270 2280 VFLLECSVRE VRVAVATILE KTLDSALFYQ DKLKSLHQLL EVLLALLDKD VPENCKNCAQ 2290 2300 2310 2320 2330 2340 YFSLFNTFVQ KQGIRAGDLL LRHSALRHMI SFLLGVSRQN SQIRRWSSAQ AREFGNLHNT 2350 2360 2370 2380 2390 2400 VALLVLHSDV SSQRNVAPGI FKQRPPISVA PSSPLLPLHE EVEALLFLSE GKPYLLEVMF 2410 2420 2430 2440 2450 2460 ALRELTGSLL ALMEMVVYCC FCNEHFSFTM LHFIKNQLET APPHELKNTF QLLHEVLVIE 2470 2480 2490 2500 2510 2520 DPIQVERVKF VFETENGLLA LMHHSNHVDS SRCYQCVKFL VTLAQKCPAA KEYFKENSHH 2530 2540 2550 2560 2570 2580 WSWAVQWLQK KMSEHYWTPQ SNVSNETSTG KTFQRTISAQ DTLAYATALL NEKEQSGSSN 2590 2600 2610 GSESSPANEN GERHLQQGSE SPMMIGELRS DLDDVDP
	Isoform 2 [UniParc]. Checksum: 21F4B4F0BAA3737F	FASTA	418	45,968
	10 20 30 40 50 60 MESEEEQHMT TLLCMGFSDP ATIRKALRLA KNDINEAVAL LTNERPGLDY GGYEPMDSGG 70 80 90 100 110 120 PSPGPGGGPR GDSGSDGSGP SRGGSTGGGG GFDPPPAYHE VVDAEKNDEN GNCSGEGIEF 130 140 150 160 170 180 PTTNLYELES RVLTDHWSIP YKREESLGKC LLASTYLARL GLSESDENCK RFMERCMPEA 190 200 210 220 230 240 FKKLLTSSAV HKWGTEIHEG IYNMLMLLIE LVAERMKQDP IPIGLLGVLT MAFNPDNEYH 250 260 270 280 290 300 FKNRMKVSQR NWAEVFGEGN MFAISPVSTF QKEPHGWVVD LVNKFGELGG FAAIQAKLHS 310 320 330 340 350 360 EDIELGAVSA LVQPLGVCAE YLNSSVVQPM LDPVILTTIQ DVRSVEEKDL KDKRLVSIPE 370 380 390 400 410 LLSAIKLLCM RFQPALVTTV DALRLDILLR MLKSPHFSAK MNSLKEVSVH LSHMWPLM

References

[1]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Strain: C57BL/6J. Tissue: Embryo.
[2]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6J.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 2384-2617 (ISOFORM 1). Strain: FVB/N. Tissue: Mammary tumor.
[4]	"Specific phosphopeptide enrichment with immobilized titanium ion affinity chromatography adsorbent for phosphoproteome analysis." Zhou H., Ye M., Dong J., Han G., Jiang X., Wu R., Zou H. J. Proteome Res. 7:3957-3967(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-2044 AND THR-2556, MASS SPECTROMETRY. Tissue: Liver.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK045043 mRNA. Translation: BAC32195.1. AL954352, AL840623 Genomic DNA. Translation: CAM15750.1. AL840623, AL954352 Genomic DNA. Translation: CAM19818.1. AL840623 Genomic DNA. Translation: CAM19819.1. BC029165 mRNA. No translation available.
IPI	IPI00123410. IPI00752867.
RefSeq	NP_899048.2. NM_183225.2.
UniGene	Mm.234544.
3D structure databases
ProteinModelPortal	B1AY13.
SMR	B1AY13. Positions 957-1031, 1648-2040.
ModBase	Search...
Protein-protein interaction databases
STRING	10090.ENSMUSP00000092538.
PTM databases
PhosphoSite	B1AY13.
Proteomic databases
PaxDb	B1AY13.
PRIDE	B1AY13.
Protocols and materials databases
DNASU	329908.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000094933; ENSMUSP00000092538; ENSMUSG00000028514. ENSMUST00000106798; ENSMUSP00000102410; ENSMUSG00000028514.
GeneID	329908.
KEGG	mmu:329908.
UCSC	uc008tyf.2. mouse. uc008tyh.2. mouse.
Organism-specific databases
CTD	23358.
MGI	MGI:1919936. Usp24.
Phylogenomic databases
eggNOG	COG5077.
GeneTree	ENSGT00650000093229.
HOGENOM	HOG000068011.
HOVERGEN	HBG105784.
InParanoid	B1AY13.
KO	K11840.
OrthoDB	EOG4T782B.
Gene expression databases
ArrayExpress	B1AY13.
Bgee	B1AY13.
Genevestigator	B1AY13.
Family and domain databases
InterPro	IPR018200. Pept_C19ubi-hydrolase_C_CS. IPR001394. Peptidase_C19. IPR009060. UBA-like. IPR015940. UBA/transl_elong_EF1B_N_euk. [Graphical view]
Pfam	PF00443. UCH. 1 hit. [Graphical view]
SUPFAM	SSF46934. UBA_like. 1 hit.
PROSITE	PS50030. UBA. 1 hit. PS00972. UCH_2_1. 1 hit. PS00973. UCH_2_2. 1 hit. PS50235. UCH_2_3. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	USP24. mouse.
NextBio	399031.
SOURCE	Search...

Entry information

Entry name

UBP24_MOUSE

Accession

Primary (citable) accession number: B1AY13
Secondary accession number(s): Q8BLI7

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 4, 2008
Last sequence update:	April 8, 2008
Last modified:	May 1, 2013
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program