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B1AY10

- NFX1_MOUSE

UniProt

B1AY10 - NFX1_MOUSE

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Protein

Transcriptional repressor NF-X1

Gene

Nfx1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli

Functioni

Binds to the X-box motif of MHC class II genes and represses their expression. May play an important role in regulating the duration of an inflammatory response by limiting the period in which MHC class II molecules are induced by interferon-gamma. Together with PABPC1 or PABPC4, acts as a coactivator for TERT expression. Mediates E2-dependent ubiquitination.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri352 – 40352RING-type; atypicalPROSITE-ProRule annotationAdd
BLAST
Zinc fingeri447 – 46519NF-X1-type 1Add
BLAST
Zinc fingeri500 – 51920NF-X1-type 2Add
BLAST
Zinc fingeri561 – 58020NF-X1-type 3Add
BLAST
Zinc fingeri626 – 64924NF-X1-type 4Add
BLAST
Zinc fingeri688 – 70720NF-X1-type 5Add
BLAST
Zinc fingeri715 – 73420NF-X1-type 6Add
BLAST
Zinc fingeri826 – 84823NF-X1-type 7Add
BLAST
Zinc fingeri857 – 87822NF-X1-type 8Add
BLAST

GO - Molecular functioni

  1. DNA binding Source: UniProtKB-KW
  2. ligase activity Source: UniProtKB-KW
  3. poly(A) RNA binding Source: Ensembl
  4. sequence-specific DNA binding transcription factor activity Source: InterPro
  5. zinc ion binding Source: InterPro

GO - Biological processi

  1. negative regulation of MHC class II biosynthetic process Source: MGI
  2. negative regulation of transcription from RNA polymerase II promoter Source: MGI
  3. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Ligase, Repressor

Keywords - Biological processi

Transcription, Transcription regulation, Ubl conjugation pathway

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Transcriptional repressor NF-X1 (EC:6.3.2.-)
Short name:
m-Nfx.1
Alternative name(s):
Nuclear transcription factor, X box-binding protein 1
Gene namesi
Name:Nfx1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 4

Organism-specific databases

MGIiMGI:1921414. Nfx1.

Subcellular locationi

Nucleus By similarity

GO - Cellular componenti

  1. nucleus Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11141114Transcriptional repressor NF-X1PRO_0000334614Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei50 – 501PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiB1AY10.
PaxDbiB1AY10.
PRIDEiB1AY10.

Expressioni

Tissue specificityi

Ubiquitously expressed, with highest levels in thymus.1 Publication

Developmental stagei

Ubiquitously expressed at E12 and E14.1 Publication

Gene expression databases

BgeeiB1AY10.
GenevestigatoriB1AY10.

Interactioni

Subunit structurei

Interacts with PABPC1 and PABPC4.By similarity

Protein-protein interaction databases

BioGridi216539. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliB1AY10.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini988 – 105669R3HPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni9 – 2618Interaction with PABPC1 and PABC4By similarityAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi1078 – 10836Poly-Pro

Domaini

The RING-type zinc finger domain interacts with an ubiquitin-conjugating enzyme (E2) and facilitates ubiquitination.By similarity

Sequence similaritiesi

Belongs to the NFX1 family.Curated
Contains 8 NF-X1-type zinc fingers.Curated
Contains 1 R3H domain.PROSITE-ProRule annotation
Contains 1 RING-type zinc finger.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri352 – 40352RING-type; atypicalPROSITE-ProRule annotationAdd
BLAST
Zinc fingeri447 – 46519NF-X1-type 1Add
BLAST
Zinc fingeri500 – 51920NF-X1-type 2Add
BLAST
Zinc fingeri561 – 58020NF-X1-type 3Add
BLAST
Zinc fingeri626 – 64924NF-X1-type 4Add
BLAST
Zinc fingeri688 – 70720NF-X1-type 5Add
BLAST
Zinc fingeri715 – 73420NF-X1-type 6Add
BLAST
Zinc fingeri826 – 84823NF-X1-type 7Add
BLAST
Zinc fingeri857 – 87822NF-X1-type 8Add
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiNOG12793.
GeneTreeiENSGT00400000022083.
HOVERGENiHBG003827.
InParanoidiB1AY10.
KOiK12236.
OMAiSKLSDMQ.
OrthoDBiEOG7NCV2M.
PhylomeDBiB1AY10.
TreeFamiTF105889.

Family and domain databases

Gene3Di3.30.1370.50. 1 hit.
InterProiIPR001374. R3H_ss-bd.
IPR019786. Zinc_finger_PHD-type_CS.
IPR000967. Znf_NFX1.
IPR019787. Znf_PHD-finger.
IPR001841. Znf_RING.
[Graphical view]
PfamiPF01424. R3H. 1 hit.
PF01422. zf-NF-X1. 4 hits.
[Graphical view]
SMARTiSM00393. R3H. 1 hit.
SM00184. RING. 1 hit.
SM00438. ZnF_NFX. 9 hits.
[Graphical view]
SUPFAMiSSF82708. SSF82708. 1 hit.
PROSITEiPS51061. R3H. 1 hit.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: B1AY10-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAEAPPVSGT FKFNTDAAEF IPQERKTSGL NCGTQRRLDS SRIGRRNYSS
60 70 80 90 100
SPPCHLPRHI PYEDISAVHQ HSYASGSKPK SPQGFFQSSN KSLKNHGLQN
110 120 130 140 150
QPWQKARNEK HQNRNKKAQG LSEQTSDTSS LESVARSESG TNPREHSPSE
160 170 180 190 200
SEKEVVIADP RGAKPKKAAQ LTYNYGRGPK AKGRLRSEWG NRMSPKSEDE
210 220 230 240 250
NTRPVAISHT DSSDASCRKP VVDPCVCRRN EQRRYPQKRP PWEVEGARPR
260 270 280 290 300
PGRNPPKQES QRHINAGPKT NMSPIPKDNL RERPTKSACD TGNLAVVSKS
310 320 330 340 350
SRRVNQEKTA VRRQDPQVLS PFPRGKQNHM LKNVETHTGS LIEQLTTEKY
360 370 380 390 400
ECMVCCELVQ VTAPVWSCQS CFHVFHLNCI KKWARSPASH ADGQSGWRCP
410 420 430 440 450
ACQNVSAHVP NTYTCFCGKV KNPEWSRNEI PHSCGEVCRK KQPGQDCPHS
460 470 480 490 500
CNLLCHPGPC PPCPAFTTKT CECGRTRHTV RCGQPVSVHC SNACENILNC
510 520 530 540 550
GQHHCAELCH GGQCQPCRII LNQVCYCGST SRDVLCGTDV GKSDGFGDFS
560 570 580 590 600
CLKICGKDLK CGSHTCSQVC HPQPCQPCPR LPHLVRYCPC GQTPLSQLLE
610 620 630 640 650
HGSNARKTCM DPVPSCGKVC GKPLACGSSD FIHTCEKLCH EGDCGPCSRT
660 670 680 690 700
SVISCRCSFR TKELPCTSLK SEDATFMCDK RCNKKRLCGR HKCNEICCVD
710 720 730 740 750
KEHKCPLICG RKLRCGLHRC EEPCHRGNCQ TCWQASFDEL TCHCGASVIY
760 770 780 790 800
PPVPCGTRPP ECTQTCARIH ECDHPVYHSC HSEEKCPPCT FLTQKWCMGK
810 820 830 840 850
HELRSNIPCH LVDISCGLPC SAMLPCGMHK CQRLCHKGEC LVDEACKQPC
860 870 880 890 900
TTPRGDCGHP CMAPCHPSLP CPVTACKAKV ELQCECGRRK EMVICSEASG
910 920 930 940 950
TYQRIVAISM ASKITDMQLG DSVEISKLIT KKEVQQARLQ CDEECAALER
960 970 980 990 1000
RKRLAEAFDI TDDSDPFNVR SSASKFSDSL KDDARKDLKF VSDVEKEMET
1010 1020 1030 1040 1050
LVEAVNKGKN NKKSHCFPPM NRDHRRIIHD LAQVYGLESI SYDSEPKRNV
1060 1070 1080 1090 1100
VVTAVRGKSV CPPTTLTSVI ERETQTRPPP PIPHHRHQAD KAPGSSTLQK
1110
IVKEAVIDYF DVQD
Length:1,114
Mass (Da):123,811
Last modified:April 8, 2008 - v1
Checksum:iA31013ACC6FB31F4
GO
Isoform 2 (identifier: B1AY10-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     803-804: LR → RQ
     805-1114: Missing.

Show »
Length:804
Mass (Da):89,439
Checksum:i0EC4111EF5F6C6E8
GO
Isoform 3 (identifier: B1AY10-3) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     803-818: LRSNIPCHLVDISCGL → ELTIKKLWTFKETLDF
     819-1114: Missing.

Show »
Length:818
Mass (Da):91,149
Checksum:iE803AE670CF16778
GO

Sequence cautioni

The sequence AAF34700.1 differs from that shown. Reason: Frameshift at positions 200, 214, 242 and 246.

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti49 – 491S → N in BAC28494. (PubMed:16141072)Curated
Sequence conflicti141 – 1411T → A in BAE33235. (PubMed:16141072)Curated
Sequence conflicti273 – 2731S → P in AAH53709. (PubMed:15489334)Curated
Sequence conflicti455 – 4551C → Y in BAE33235. (PubMed:16141072)Curated
Sequence conflicti590 – 5901C → R in AAH53709. (PubMed:15489334)Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei803 – 81816LRSNI…ISCGL → ELTIKKLWTFKETLDF in isoform 3. 1 PublicationVSP_033693Add
BLAST
Alternative sequencei803 – 8042LR → RQ in isoform 2. 2 PublicationsVSP_033694
Alternative sequencei805 – 1114310Missing in isoform 2. 2 PublicationsVSP_033695Add
BLAST
Alternative sequencei819 – 1114296Missing in isoform 3. 1 PublicationVSP_033696Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF223576 mRNA. Translation: AAF34700.1. Frameshift.
AK005038 mRNA. Translation: BAB23765.1.
AK033850 mRNA. Translation: BAC28494.1.
AK144647 mRNA. Translation: BAE25987.1.
AK146108 mRNA. Translation: BAE26907.1.
AK155387 mRNA. Translation: BAE33235.1.
AL837521 Genomic DNA. Translation: CAM19845.1.
AL837521 Genomic DNA. Translation: CAM19846.1.
AL837521 Genomic DNA. Translation: CAM19847.1.
BC053709 mRNA. Translation: AAH53709.1.
CCDSiCCDS18054.1. [B1AY10-1]
CCDS71359.1. [B1AY10-2]
CCDS71360.1. [B1AY10-3]
RefSeqiNP_001277377.1. NM_001290448.1. [B1AY10-3]
NP_001277378.1. NM_001290449.1. [B1AY10-2]
NP_076228.2. NM_023739.3. [B1AY10-1]
UniGeneiMm.247456.
Mm.439181.

Genome annotation databases

EnsembliENSMUST00000030133; ENSMUSP00000030133; ENSMUSG00000028423. [B1AY10-3]
ENSMUST00000091614; ENSMUSP00000089203; ENSMUSG00000028423. [B1AY10-2]
ENSMUST00000098143; ENSMUSP00000095747; ENSMUSG00000028423. [B1AY10-1]
GeneIDi74164.
KEGGimmu:74164.
UCSCiuc008sic.1. mouse. [B1AY10-2]
uc008sid.1. mouse. [B1AY10-3]
uc008sie.1. mouse. [B1AY10-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF223576 mRNA. Translation: AAF34700.1 . Frameshift.
AK005038 mRNA. Translation: BAB23765.1 .
AK033850 mRNA. Translation: BAC28494.1 .
AK144647 mRNA. Translation: BAE25987.1 .
AK146108 mRNA. Translation: BAE26907.1 .
AK155387 mRNA. Translation: BAE33235.1 .
AL837521 Genomic DNA. Translation: CAM19845.1 .
AL837521 Genomic DNA. Translation: CAM19846.1 .
AL837521 Genomic DNA. Translation: CAM19847.1 .
BC053709 mRNA. Translation: AAH53709.1 .
CCDSi CCDS18054.1. [B1AY10-1 ]
CCDS71359.1. [B1AY10-2 ]
CCDS71360.1. [B1AY10-3 ]
RefSeqi NP_001277377.1. NM_001290448.1. [B1AY10-3 ]
NP_001277378.1. NM_001290449.1. [B1AY10-2 ]
NP_076228.2. NM_023739.3. [B1AY10-1 ]
UniGenei Mm.247456.
Mm.439181.

3D structure databases

ProteinModelPortali B1AY10.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 216539. 1 interaction.

Proteomic databases

MaxQBi B1AY10.
PaxDbi B1AY10.
PRIDEi B1AY10.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000030133 ; ENSMUSP00000030133 ; ENSMUSG00000028423 . [B1AY10-3 ]
ENSMUST00000091614 ; ENSMUSP00000089203 ; ENSMUSG00000028423 . [B1AY10-2 ]
ENSMUST00000098143 ; ENSMUSP00000095747 ; ENSMUSG00000028423 . [B1AY10-1 ]
GeneIDi 74164.
KEGGi mmu:74164.
UCSCi uc008sic.1. mouse. [B1AY10-2 ]
uc008sid.1. mouse. [B1AY10-3 ]
uc008sie.1. mouse. [B1AY10-1 ]

Organism-specific databases

CTDi 4799.
MGIi MGI:1921414. Nfx1.

Phylogenomic databases

eggNOGi NOG12793.
GeneTreei ENSGT00400000022083.
HOVERGENi HBG003827.
InParanoidi B1AY10.
KOi K12236.
OMAi SKLSDMQ.
OrthoDBi EOG7NCV2M.
PhylomeDBi B1AY10.
TreeFami TF105889.

Miscellaneous databases

ChiTaRSi NFX1. mouse.
NextBioi 339960.
PROi B1AY10.
SOURCEi Search...

Gene expression databases

Bgeei B1AY10.
Genevestigatori B1AY10.

Family and domain databases

Gene3Di 3.30.1370.50. 1 hit.
InterProi IPR001374. R3H_ss-bd.
IPR019786. Zinc_finger_PHD-type_CS.
IPR000967. Znf_NFX1.
IPR019787. Znf_PHD-finger.
IPR001841. Znf_RING.
[Graphical view ]
Pfami PF01424. R3H. 1 hit.
PF01422. zf-NF-X1. 4 hits.
[Graphical view ]
SMARTi SM00393. R3H. 1 hit.
SM00184. RING. 1 hit.
SM00438. ZnF_NFX. 9 hits.
[Graphical view ]
SUPFAMi SSF82708. SSF82708. 1 hit.
PROSITEi PS51061. R3H. 1 hit.
PS50089. ZF_RING_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Murine NFX.1: isolation and characterization of its messenger RNA, mapping of its chromosomal location and assessment of its developmental expression."
    Arlotta P., Miyazaki D., Copeland N.G., Gilbert D.J., Jenkins N.A., Ono S.J.
    Immunology 106:173-181(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1; 2 AND 3).
    Strain: C57BL/6J and NOD.
    Tissue: Diencephalon, Liver, Lung and Placenta.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Strain: C57BL/6J.
    Tissue: Egg.

Entry informationi

Entry nameiNFX1_MOUSE
AccessioniPrimary (citable) accession number: B1AY10
Secondary accession number(s): Q3U2A7
, Q3UK95, Q3UMW1, Q7TPT4, Q8CC59, Q9DBC8, Q9JKW7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: April 8, 2008
Last modified: October 29, 2014
This is version 58 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3