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B1AY10 (NFX1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 55. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Transcriptional repressor NF-X1

Short name=m-Nfx.1
EC=6.3.2.-
Alternative name(s):
Nuclear transcription factor, X box-binding protein 1
Gene names
Name:Nfx1
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length1114 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Binds to the X-box motif of MHC class II genes and represses their expression. May play an important role in regulating the duration of an inflammatory response by limiting the period in which MHC class II molecules are induced by interferon-gamma. Together with PABPC1 or PABPC4, acts as a coactivator for TERT expression. Mediates E2-dependent ubiquitination. Ref.1

Subunit structure

Interacts with PABPC1 and PABPC4 By similarity.

Subcellular location

Nucleus By similarity.

Tissue specificity

Ubiquitously expressed, with highest levels in thymus. Ref.1

Developmental stage

Ubiquitously expressed at E12 and E14. Ref.1

Domain

The RING-type zinc finger domain interacts with an ubiquitin-conjugating enzyme (E2) and facilitates ubiquitination By similarity.

Sequence similarities

Belongs to the NFX1 family.

Contains 8 NF-X1-type zinc fingers.

Contains 1 R3H domain.

Contains 1 RING-type zinc finger.

Sequence caution

The sequence AAF34700.1 differs from that shown. Reason: Frameshift at positions 200, 214, 242 and 246.

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: B1AY10-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: B1AY10-2)

The sequence of this isoform differs from the canonical sequence as follows:
     803-804: LR → RQ
     805-1114: Missing.
Isoform 3 (identifier: B1AY10-3)

The sequence of this isoform differs from the canonical sequence as follows:
     803-818: LRSNIPCHLVDISCGL → ELTIKKLWTFKETLDF
     819-1114: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 11141114Transcriptional repressor NF-X1
PRO_0000334614

Regions

Domain988 – 105669R3H
Zinc finger352 – 40352RING-type; atypical
Zinc finger447 – 46519NF-X1-type 1
Zinc finger500 – 51920NF-X1-type 2
Zinc finger561 – 58020NF-X1-type 3
Zinc finger626 – 64924NF-X1-type 4
Zinc finger688 – 70720NF-X1-type 5
Zinc finger715 – 73420NF-X1-type 6
Zinc finger826 – 84823NF-X1-type 7
Zinc finger857 – 87822NF-X1-type 8
Region9 – 2618Interaction with PABPC1 and PABC4 By similarity
Compositional bias1078 – 10836Poly-Pro

Amino acid modifications

Modified residue501Phosphoserine By similarity

Natural variations

Alternative sequence803 – 81816LRSNI…ISCGL → ELTIKKLWTFKETLDF in isoform 3.
VSP_033693
Alternative sequence803 – 8042LR → RQ in isoform 2.
VSP_033694
Alternative sequence805 – 1114310Missing in isoform 2.
VSP_033695
Alternative sequence819 – 1114296Missing in isoform 3.
VSP_033696

Experimental info

Sequence conflict491S → N in BAC28494. Ref.2
Sequence conflict1411T → A in BAE33235. Ref.2
Sequence conflict2731S → P in AAH53709. Ref.4
Sequence conflict4551C → Y in BAE33235. Ref.2
Sequence conflict5901C → R in AAH53709. Ref.4

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified April 8, 2008. Version 1.
Checksum: A31013ACC6FB31F4

FASTA1,114123,811
        10         20         30         40         50         60 
MAEAPPVSGT FKFNTDAAEF IPQERKTSGL NCGTQRRLDS SRIGRRNYSS SPPCHLPRHI 

        70         80         90        100        110        120 
PYEDISAVHQ HSYASGSKPK SPQGFFQSSN KSLKNHGLQN QPWQKARNEK HQNRNKKAQG 

       130        140        150        160        170        180 
LSEQTSDTSS LESVARSESG TNPREHSPSE SEKEVVIADP RGAKPKKAAQ LTYNYGRGPK 

       190        200        210        220        230        240 
AKGRLRSEWG NRMSPKSEDE NTRPVAISHT DSSDASCRKP VVDPCVCRRN EQRRYPQKRP 

       250        260        270        280        290        300 
PWEVEGARPR PGRNPPKQES QRHINAGPKT NMSPIPKDNL RERPTKSACD TGNLAVVSKS 

       310        320        330        340        350        360 
SRRVNQEKTA VRRQDPQVLS PFPRGKQNHM LKNVETHTGS LIEQLTTEKY ECMVCCELVQ 

       370        380        390        400        410        420 
VTAPVWSCQS CFHVFHLNCI KKWARSPASH ADGQSGWRCP ACQNVSAHVP NTYTCFCGKV 

       430        440        450        460        470        480 
KNPEWSRNEI PHSCGEVCRK KQPGQDCPHS CNLLCHPGPC PPCPAFTTKT CECGRTRHTV 

       490        500        510        520        530        540 
RCGQPVSVHC SNACENILNC GQHHCAELCH GGQCQPCRII LNQVCYCGST SRDVLCGTDV 

       550        560        570        580        590        600 
GKSDGFGDFS CLKICGKDLK CGSHTCSQVC HPQPCQPCPR LPHLVRYCPC GQTPLSQLLE 

       610        620        630        640        650        660 
HGSNARKTCM DPVPSCGKVC GKPLACGSSD FIHTCEKLCH EGDCGPCSRT SVISCRCSFR 

       670        680        690        700        710        720 
TKELPCTSLK SEDATFMCDK RCNKKRLCGR HKCNEICCVD KEHKCPLICG RKLRCGLHRC 

       730        740        750        760        770        780 
EEPCHRGNCQ TCWQASFDEL TCHCGASVIY PPVPCGTRPP ECTQTCARIH ECDHPVYHSC 

       790        800        810        820        830        840 
HSEEKCPPCT FLTQKWCMGK HELRSNIPCH LVDISCGLPC SAMLPCGMHK CQRLCHKGEC 

       850        860        870        880        890        900 
LVDEACKQPC TTPRGDCGHP CMAPCHPSLP CPVTACKAKV ELQCECGRRK EMVICSEASG 

       910        920        930        940        950        960 
TYQRIVAISM ASKITDMQLG DSVEISKLIT KKEVQQARLQ CDEECAALER RKRLAEAFDI 

       970        980        990       1000       1010       1020 
TDDSDPFNVR SSASKFSDSL KDDARKDLKF VSDVEKEMET LVEAVNKGKN NKKSHCFPPM 

      1030       1040       1050       1060       1070       1080 
NRDHRRIIHD LAQVYGLESI SYDSEPKRNV VVTAVRGKSV CPPTTLTSVI ERETQTRPPP 

      1090       1100       1110 
PIPHHRHQAD KAPGSSTLQK IVKEAVIDYF DVQD 

« Hide

Isoform 2 [UniParc].

Checksum: 0EC4111EF5F6C6E8
Show »

FASTA80489,439
Isoform 3 [UniParc].

Checksum: E803AE670CF16778
Show »

FASTA81891,149

References

« Hide 'large scale' references
[1]"Murine NFX.1: isolation and characterization of its messenger RNA, mapping of its chromosomal location and assessment of its developmental expression."
Arlotta P., Miyazaki D., Copeland N.G., Gilbert D.J., Jenkins N.A., Ono S.J.
Immunology 106:173-181(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
[2]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1; 2 AND 3).
Strain: C57BL/6J and NOD.
Tissue: Diencephalon, Liver, Lung and Placenta.
[3]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Strain: C57BL/6J.
Tissue: Egg.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF223576 mRNA. Translation: AAF34700.1. Frameshift.
AK005038 mRNA. Translation: BAB23765.1.
AK033850 mRNA. Translation: BAC28494.1.
AK144647 mRNA. Translation: BAE25987.1.
AK146108 mRNA. Translation: BAE26907.1.
AK155387 mRNA. Translation: BAE33235.1.
AL837521 Genomic DNA. Translation: CAM19845.1.
AL837521 Genomic DNA. Translation: CAM19846.1.
AL837521 Genomic DNA. Translation: CAM19847.1.
BC053709 mRNA. Translation: AAH53709.1.
CCDSCCDS18054.1. [B1AY10-1]
RefSeqNP_001277377.1. NM_001290448.1. [B1AY10-3]
NP_001277378.1. NM_001290449.1. [B1AY10-2]
NP_076228.2. NM_023739.3. [B1AY10-1]
UniGeneMm.247456.
Mm.439181.

3D structure databases

ProteinModelPortalB1AY10.
SMRB1AY10. Positions 348-407.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid216539. 1 interaction.

Proteomic databases

PaxDbB1AY10.
PRIDEB1AY10.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000030133; ENSMUSP00000030133; ENSMUSG00000028423. [B1AY10-3]
ENSMUST00000091614; ENSMUSP00000089203; ENSMUSG00000028423. [B1AY10-2]
ENSMUST00000098143; ENSMUSP00000095747; ENSMUSG00000028423. [B1AY10-1]
GeneID74164.
KEGGmmu:74164.
UCSCuc008sic.1. mouse. [B1AY10-2]
uc008sid.1. mouse. [B1AY10-3]
uc008sie.1. mouse. [B1AY10-1]

Organism-specific databases

CTD4799.
MGIMGI:1921414. Nfx1.

Phylogenomic databases

eggNOGNOG12793.
GeneTreeENSGT00400000022083.
HOVERGENHBG003827.
InParanoidB1AY10.
KOK12236.
OMASKLSDMQ.
OrthoDBEOG7NCV2M.
PhylomeDBB1AY10.
TreeFamTF105889.

Gene expression databases

BgeeB1AY10.
GenevestigatorB1AY10.

Family and domain databases

Gene3D3.30.1370.50. 1 hit.
InterProIPR001374. R3H_ss-bd.
IPR019786. Zinc_finger_PHD-type_CS.
IPR000967. Znf_NFX1.
IPR019787. Znf_PHD-finger.
IPR001841. Znf_RING.
[Graphical view]
PfamPF01424. R3H. 1 hit.
PF01422. zf-NF-X1. 4 hits.
[Graphical view]
SMARTSM00393. R3H. 1 hit.
SM00184. RING. 1 hit.
SM00438. ZnF_NFX. 9 hits.
[Graphical view]
SUPFAMSSF82708. SSF82708. 1 hit.
PROSITEPS51061. R3H. 1 hit.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSNFX1. mouse.
NextBio339960.
PROB1AY10.
SOURCESearch...

Entry information

Entry nameNFX1_MOUSE
AccessionPrimary (citable) accession number: B1AY10
Secondary accession number(s): Q3U2A7 expand/collapse secondary AC list , Q3UK95, Q3UMW1, Q7TPT4, Q8CC59, Q9DBC8, Q9JKW7
Entry history
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: April 8, 2008
Last modified: July 9, 2014
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot