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B1AY10

- NFX1_MOUSE

UniProt

B1AY10 - NFX1_MOUSE

Protein

Transcriptional repressor NF-X1

Gene

Nfx1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 57 (01 Oct 2014)
      Sequence version 1 (08 Apr 2008)
      Previous versions | rss
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    Functioni

    Binds to the X-box motif of MHC class II genes and represses their expression. May play an important role in regulating the duration of an inflammatory response by limiting the period in which MHC class II molecules are induced by interferon-gamma. Together with PABPC1 or PABPC4, acts as a coactivator for TERT expression. Mediates E2-dependent ubiquitination.1 Publication

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri352 – 40352RING-type; atypicalPROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri447 – 46519NF-X1-type 1Add
    BLAST
    Zinc fingeri500 – 51920NF-X1-type 2Add
    BLAST
    Zinc fingeri561 – 58020NF-X1-type 3Add
    BLAST
    Zinc fingeri626 – 64924NF-X1-type 4Add
    BLAST
    Zinc fingeri688 – 70720NF-X1-type 5Add
    BLAST
    Zinc fingeri715 – 73420NF-X1-type 6Add
    BLAST
    Zinc fingeri826 – 84823NF-X1-type 7Add
    BLAST
    Zinc fingeri857 – 87822NF-X1-type 8Add
    BLAST

    GO - Molecular functioni

    1. DNA binding Source: UniProtKB-KW
    2. ligase activity Source: UniProtKB-KW
    3. protein binding Source: MGI
    4. sequence-specific DNA binding transcription factor activity Source: InterPro
    5. zinc ion binding Source: InterPro

    GO - Biological processi

    1. negative regulation of MHC class II biosynthetic process Source: MGI
    2. negative regulation of transcription from RNA polymerase II promoter Source: MGI
    3. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Ligase, Repressor

    Keywords - Biological processi

    Transcription, Transcription regulation, Ubl conjugation pathway

    Keywords - Ligandi

    DNA-binding, Metal-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Transcriptional repressor NF-X1 (EC:6.3.2.-)
    Short name:
    m-Nfx.1
    Alternative name(s):
    Nuclear transcription factor, X box-binding protein 1
    Gene namesi
    Name:Nfx1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 4

    Organism-specific databases

    MGIiMGI:1921414. Nfx1.

    Subcellular locationi

    Nucleus By similarity

    GO - Cellular componenti

    1. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 11141114Transcriptional repressor NF-X1PRO_0000334614Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei50 – 501PhosphoserineBy similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiB1AY10.
    PRIDEiB1AY10.

    Expressioni

    Tissue specificityi

    Ubiquitously expressed, with highest levels in thymus.1 Publication

    Developmental stagei

    Ubiquitously expressed at E12 and E14.1 Publication

    Gene expression databases

    BgeeiB1AY10.
    GenevestigatoriB1AY10.

    Interactioni

    Subunit structurei

    Interacts with PABPC1 and PABPC4.By similarity

    Protein-protein interaction databases

    BioGridi216539. 1 interaction.

    Structurei

    3D structure databases

    ProteinModelPortaliB1AY10.
    SMRiB1AY10. Positions 348-407.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini988 – 105669R3HPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni9 – 2618Interaction with PABPC1 and PABC4By similarityAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi1078 – 10836Poly-Pro

    Domaini

    The RING-type zinc finger domain interacts with an ubiquitin-conjugating enzyme (E2) and facilitates ubiquitination.By similarity

    Sequence similaritiesi

    Belongs to the NFX1 family.Curated
    Contains 8 NF-X1-type zinc fingers.Curated
    Contains 1 R3H domain.PROSITE-ProRule annotation
    Contains 1 RING-type zinc finger.PROSITE-ProRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri352 – 40352RING-type; atypicalPROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri447 – 46519NF-X1-type 1Add
    BLAST
    Zinc fingeri500 – 51920NF-X1-type 2Add
    BLAST
    Zinc fingeri561 – 58020NF-X1-type 3Add
    BLAST
    Zinc fingeri626 – 64924NF-X1-type 4Add
    BLAST
    Zinc fingeri688 – 70720NF-X1-type 5Add
    BLAST
    Zinc fingeri715 – 73420NF-X1-type 6Add
    BLAST
    Zinc fingeri826 – 84823NF-X1-type 7Add
    BLAST
    Zinc fingeri857 – 87822NF-X1-type 8Add
    BLAST

    Keywords - Domaini

    Repeat, Zinc-finger

    Phylogenomic databases

    eggNOGiNOG12793.
    GeneTreeiENSGT00400000022083.
    HOVERGENiHBG003827.
    InParanoidiB1AY10.
    KOiK12236.
    OMAiSKLSDMQ.
    OrthoDBiEOG7NCV2M.
    PhylomeDBiB1AY10.
    TreeFamiTF105889.

    Family and domain databases

    Gene3Di3.30.1370.50. 1 hit.
    InterProiIPR001374. R3H_ss-bd.
    IPR019786. Zinc_finger_PHD-type_CS.
    IPR000967. Znf_NFX1.
    IPR019787. Znf_PHD-finger.
    IPR001841. Znf_RING.
    [Graphical view]
    PfamiPF01424. R3H. 1 hit.
    PF01422. zf-NF-X1. 4 hits.
    [Graphical view]
    SMARTiSM00393. R3H. 1 hit.
    SM00184. RING. 1 hit.
    SM00438. ZnF_NFX. 9 hits.
    [Graphical view]
    SUPFAMiSSF82708. SSF82708. 1 hit.
    PROSITEiPS51061. R3H. 1 hit.
    PS50089. ZF_RING_2. 1 hit.
    [Graphical view]

    Sequences (3)i

    Sequence statusi: Complete.

    This entry describes 3 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: B1AY10-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MAEAPPVSGT FKFNTDAAEF IPQERKTSGL NCGTQRRLDS SRIGRRNYSS     50
    SPPCHLPRHI PYEDISAVHQ HSYASGSKPK SPQGFFQSSN KSLKNHGLQN 100
    QPWQKARNEK HQNRNKKAQG LSEQTSDTSS LESVARSESG TNPREHSPSE 150
    SEKEVVIADP RGAKPKKAAQ LTYNYGRGPK AKGRLRSEWG NRMSPKSEDE 200
    NTRPVAISHT DSSDASCRKP VVDPCVCRRN EQRRYPQKRP PWEVEGARPR 250
    PGRNPPKQES QRHINAGPKT NMSPIPKDNL RERPTKSACD TGNLAVVSKS 300
    SRRVNQEKTA VRRQDPQVLS PFPRGKQNHM LKNVETHTGS LIEQLTTEKY 350
    ECMVCCELVQ VTAPVWSCQS CFHVFHLNCI KKWARSPASH ADGQSGWRCP 400
    ACQNVSAHVP NTYTCFCGKV KNPEWSRNEI PHSCGEVCRK KQPGQDCPHS 450
    CNLLCHPGPC PPCPAFTTKT CECGRTRHTV RCGQPVSVHC SNACENILNC 500
    GQHHCAELCH GGQCQPCRII LNQVCYCGST SRDVLCGTDV GKSDGFGDFS 550
    CLKICGKDLK CGSHTCSQVC HPQPCQPCPR LPHLVRYCPC GQTPLSQLLE 600
    HGSNARKTCM DPVPSCGKVC GKPLACGSSD FIHTCEKLCH EGDCGPCSRT 650
    SVISCRCSFR TKELPCTSLK SEDATFMCDK RCNKKRLCGR HKCNEICCVD 700
    KEHKCPLICG RKLRCGLHRC EEPCHRGNCQ TCWQASFDEL TCHCGASVIY 750
    PPVPCGTRPP ECTQTCARIH ECDHPVYHSC HSEEKCPPCT FLTQKWCMGK 800
    HELRSNIPCH LVDISCGLPC SAMLPCGMHK CQRLCHKGEC LVDEACKQPC 850
    TTPRGDCGHP CMAPCHPSLP CPVTACKAKV ELQCECGRRK EMVICSEASG 900
    TYQRIVAISM ASKITDMQLG DSVEISKLIT KKEVQQARLQ CDEECAALER 950
    RKRLAEAFDI TDDSDPFNVR SSASKFSDSL KDDARKDLKF VSDVEKEMET 1000
    LVEAVNKGKN NKKSHCFPPM NRDHRRIIHD LAQVYGLESI SYDSEPKRNV 1050
    VVTAVRGKSV CPPTTLTSVI ERETQTRPPP PIPHHRHQAD KAPGSSTLQK 1100
    IVKEAVIDYF DVQD 1114
    Length:1,114
    Mass (Da):123,811
    Last modified:April 8, 2008 - v1
    Checksum:iA31013ACC6FB31F4
    GO
    Isoform 2 (identifier: B1AY10-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         803-804: LR → RQ
         805-1114: Missing.

    Show »
    Length:804
    Mass (Da):89,439
    Checksum:i0EC4111EF5F6C6E8
    GO
    Isoform 3 (identifier: B1AY10-3) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         803-818: LRSNIPCHLVDISCGL → ELTIKKLWTFKETLDF
         819-1114: Missing.

    Show »
    Length:818
    Mass (Da):91,149
    Checksum:iE803AE670CF16778
    GO

    Sequence cautioni

    The sequence AAF34700.1 differs from that shown. Reason: Frameshift at positions 200, 214, 242 and 246.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti49 – 491S → N in BAC28494. (PubMed:16141072)Curated
    Sequence conflicti141 – 1411T → A in BAE33235. (PubMed:16141072)Curated
    Sequence conflicti273 – 2731S → P in AAH53709. (PubMed:15489334)Curated
    Sequence conflicti455 – 4551C → Y in BAE33235. (PubMed:16141072)Curated
    Sequence conflicti590 – 5901C → R in AAH53709. (PubMed:15489334)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei803 – 81816LRSNI…ISCGL → ELTIKKLWTFKETLDF in isoform 3. 1 PublicationVSP_033693Add
    BLAST
    Alternative sequencei803 – 8042LR → RQ in isoform 2. 2 PublicationsVSP_033694
    Alternative sequencei805 – 1114310Missing in isoform 2. 2 PublicationsVSP_033695Add
    BLAST
    Alternative sequencei819 – 1114296Missing in isoform 3. 1 PublicationVSP_033696Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF223576 mRNA. Translation: AAF34700.1. Frameshift.
    AK005038 mRNA. Translation: BAB23765.1.
    AK033850 mRNA. Translation: BAC28494.1.
    AK144647 mRNA. Translation: BAE25987.1.
    AK146108 mRNA. Translation: BAE26907.1.
    AK155387 mRNA. Translation: BAE33235.1.
    AL837521 Genomic DNA. Translation: CAM19845.1.
    AL837521 Genomic DNA. Translation: CAM19846.1.
    AL837521 Genomic DNA. Translation: CAM19847.1.
    BC053709 mRNA. Translation: AAH53709.1.
    CCDSiCCDS18054.1. [B1AY10-1]
    CCDS71359.1. [B1AY10-2]
    CCDS71360.1. [B1AY10-3]
    RefSeqiNP_001277377.1. NM_001290448.1. [B1AY10-3]
    NP_001277378.1. NM_001290449.1. [B1AY10-2]
    NP_076228.2. NM_023739.3. [B1AY10-1]
    UniGeneiMm.247456.
    Mm.439181.

    Genome annotation databases

    EnsembliENSMUST00000030133; ENSMUSP00000030133; ENSMUSG00000028423. [B1AY10-3]
    ENSMUST00000091614; ENSMUSP00000089203; ENSMUSG00000028423. [B1AY10-2]
    ENSMUST00000098143; ENSMUSP00000095747; ENSMUSG00000028423. [B1AY10-1]
    GeneIDi74164.
    KEGGimmu:74164.
    UCSCiuc008sic.1. mouse. [B1AY10-2]
    uc008sid.1. mouse. [B1AY10-3]
    uc008sie.1. mouse. [B1AY10-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF223576 mRNA. Translation: AAF34700.1 . Frameshift.
    AK005038 mRNA. Translation: BAB23765.1 .
    AK033850 mRNA. Translation: BAC28494.1 .
    AK144647 mRNA. Translation: BAE25987.1 .
    AK146108 mRNA. Translation: BAE26907.1 .
    AK155387 mRNA. Translation: BAE33235.1 .
    AL837521 Genomic DNA. Translation: CAM19845.1 .
    AL837521 Genomic DNA. Translation: CAM19846.1 .
    AL837521 Genomic DNA. Translation: CAM19847.1 .
    BC053709 mRNA. Translation: AAH53709.1 .
    CCDSi CCDS18054.1. [B1AY10-1 ]
    CCDS71359.1. [B1AY10-2 ]
    CCDS71360.1. [B1AY10-3 ]
    RefSeqi NP_001277377.1. NM_001290448.1. [B1AY10-3 ]
    NP_001277378.1. NM_001290449.1. [B1AY10-2 ]
    NP_076228.2. NM_023739.3. [B1AY10-1 ]
    UniGenei Mm.247456.
    Mm.439181.

    3D structure databases

    ProteinModelPortali B1AY10.
    SMRi B1AY10. Positions 348-407.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 216539. 1 interaction.

    Proteomic databases

    PaxDbi B1AY10.
    PRIDEi B1AY10.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000030133 ; ENSMUSP00000030133 ; ENSMUSG00000028423 . [B1AY10-3 ]
    ENSMUST00000091614 ; ENSMUSP00000089203 ; ENSMUSG00000028423 . [B1AY10-2 ]
    ENSMUST00000098143 ; ENSMUSP00000095747 ; ENSMUSG00000028423 . [B1AY10-1 ]
    GeneIDi 74164.
    KEGGi mmu:74164.
    UCSCi uc008sic.1. mouse. [B1AY10-2 ]
    uc008sid.1. mouse. [B1AY10-3 ]
    uc008sie.1. mouse. [B1AY10-1 ]

    Organism-specific databases

    CTDi 4799.
    MGIi MGI:1921414. Nfx1.

    Phylogenomic databases

    eggNOGi NOG12793.
    GeneTreei ENSGT00400000022083.
    HOVERGENi HBG003827.
    InParanoidi B1AY10.
    KOi K12236.
    OMAi SKLSDMQ.
    OrthoDBi EOG7NCV2M.
    PhylomeDBi B1AY10.
    TreeFami TF105889.

    Miscellaneous databases

    ChiTaRSi NFX1. mouse.
    NextBioi 339960.
    PROi B1AY10.
    SOURCEi Search...

    Gene expression databases

    Bgeei B1AY10.
    Genevestigatori B1AY10.

    Family and domain databases

    Gene3Di 3.30.1370.50. 1 hit.
    InterProi IPR001374. R3H_ss-bd.
    IPR019786. Zinc_finger_PHD-type_CS.
    IPR000967. Znf_NFX1.
    IPR019787. Znf_PHD-finger.
    IPR001841. Znf_RING.
    [Graphical view ]
    Pfami PF01424. R3H. 1 hit.
    PF01422. zf-NF-X1. 4 hits.
    [Graphical view ]
    SMARTi SM00393. R3H. 1 hit.
    SM00184. RING. 1 hit.
    SM00438. ZnF_NFX. 9 hits.
    [Graphical view ]
    SUPFAMi SSF82708. SSF82708. 1 hit.
    PROSITEi PS51061. R3H. 1 hit.
    PS50089. ZF_RING_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Murine NFX.1: isolation and characterization of its messenger RNA, mapping of its chromosomal location and assessment of its developmental expression."
      Arlotta P., Miyazaki D., Copeland N.G., Gilbert D.J., Jenkins N.A., Ono S.J.
      Immunology 106:173-181(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1; 2 AND 3).
      Strain: C57BL/6J and NOD.
      Tissue: Diencephalon, Liver, Lung and Placenta.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
      Strain: C57BL/6J.
      Tissue: Egg.

    Entry informationi

    Entry nameiNFX1_MOUSE
    AccessioniPrimary (citable) accession number: B1AY10
    Secondary accession number(s): Q3U2A7
    , Q3UK95, Q3UMW1, Q7TPT4, Q8CC59, Q9DBC8, Q9JKW7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 20, 2008
    Last sequence update: April 8, 2008
    Last modified: October 1, 2014
    This is version 57 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3