B1AVY7 (KI16B_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Kinesin-like protein KIF16B

Gene names

Name:	Kif16b
Synonyms:	Kiaa1590

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	1312 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Plus end-directed microtubule-dependent motor protein involved in endosome transport and receptor recycling and degradation. Regulates the plus end motility of early endosomes and the balance between recycling and degradation of receptors such as EGF receptor (EGFR) and FGF receptor (FGFR). Regulates the Golgi to endosome transport of FGFR-containing vesicles during early development, a key process for developing basement membrane and epiblast and primitive endoderm lineages during early postimplantation development. Ref.6
Subunit structure	Interacts with PTPN21 By similarity. Interacts with RAB14. Ref.6
Subcellular location	Cytoplasm › cytoskeleton By similarity. Early endosome membrane By similarity. Note: It is unclear whether association with endosomes is mediated via phosphatidylinositol 3-phosphate (PtdIns3P)-binding or via its interaction with RAB14.
Domain	The PX domain mediates binding to phosphatidylinositol 3-phosphate (PtdIns3P), phosphatidylinositol 3,4-bisphosphate (PtdIns(3,4)P2), phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2) and phosphatidylinositol 3,4,5-trisphosphate (PtdIns(3,4,5)P3). Does not bind phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2) By similarity.
Disruption phenotype	Embryonic death. Embryos are arrested at the blastocyst stage: the primitive endoderm and epiblast cannot be distinguished and appear as cell clumps resembling the inner cell mass (ICM) of the blastocyst. Embryos do not develop an epiblast epithelium and the uterine reaction appears to be incomplete. Development of the primitive endoderm and a basement membrane derived from it are severely affected in embryos at 4.5 dpc. Ref.6
Sequence similarities	Belongs to the kinesin-like protein family. Contains 1 FHA domain. Contains 1 kinesin-motor domain. Contains 1 PX (phox homology) domain.
Sequence caution	The sequence BAC98211.1 differs from that shown. Reason: Partially unspliced pre-RNA.

Ontologies

Keywords
Biological process	Transport
Cellular component	Cytoplasm Cytoskeleton Endosome Membrane Microtubule
Domain	Coiled coil
Ligand	ATP-binding Lipid-binding Nucleotide-binding
Molecular function	Motor protein
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	Golgi to endosome transport Inferred from mutant phenotype Ref.6. Source: UniProtKB early endosome to late endosome transport Inferred from sequence or structural similarity. Source: UniProtKB endoderm development Inferred from mutant phenotype Ref.6. Source: UniProtKB epidermal growth factor receptor signaling pathway Inferred from sequence or structural similarity. Source: UniProtKB fibroblast growth factor receptor signaling pathway Inferred from mutant phenotype Ref.6. Source: UniProtKB formation of primary germ layer Inferred from mutant phenotype Ref.6. Source: UniProtKB microtubule-based movement Inferred from electronic annotation. Source: InterPro receptor catabolic process Inferred from sequence or structural similarity. Source: UniProtKB regulation of receptor recycling Inferred from sequence or structural similarity. Source: UniProtKB
Cellular_component	early endosome Inferred from sequence or structural similarity. Source: UniProtKB early endosome membrane Inferred from electronic annotation. Source: UniProtKB-SubCell microtubule Inferred from electronic annotation. Source: UniProtKB-KW microtubule associated complex Inferred from electronic annotation. Source: InterPro
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphatidylinositol-3,4,5-trisphosphate binding Inferred from sequence or structural similarity. Source: UniProtKB phosphatidylinositol-3,4-bisphosphate binding Inferred from sequence or structural similarity. Source: UniProtKB phosphatidylinositol-3,5-bisphosphate binding Inferred from sequence or structural similarity. Source: UniProtKB phosphatidylinositol-3-phosphate binding Inferred from sequence or structural similarity. Source: UniProtKB plus-end-directed microtubule motor activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1312	1312	Kinesin-like protein KIF16B		PRO_0000409500
Regions
	Domain	1 – 277	277	Kinesin-motor
	Domain	?480 – ?544	65	FHA
	Domain	1177 – 1291	115	PX
	Nucleotide binding	102 – 109	8	ATP Potential
	Coiled coil	366 – 425	60	Potential
	Coiled coil	835 – 913	79	Potential
	Coiled coil	941 – 1073	133	Potential
	Compositional bias	597 – 798	202	Glu-rich
Amino acid modifications
	Modified residue	1145	1	Phosphoserine Ref.5

Sequences

	Sequence		Length	Mass (Da)
	B1AVY7 [UniParc]. Last modified April 8, 2008. Version 1. Checksum: 4397E49FF4E93881	FASTA	1,312	150,058
	10 20 30 40 50 60 MASVKVAVRV RPMNRREKDL EAKFIIQMEK SKTTITNLKI PEGGTGDSGR ERTKTFTYDF 70 80 90 100 110 120 SFYSADTKSP DYVSQEMVFK TLGTDVVKSA FEGYNACVFA YGQTGSGKSY TMMGNSGDSG 130 140 150 160 170 180 LIPRICEALF SRINETTRWD EASFRTEVSY LEIYNERVRD LLRRKSSKTF NLRVREHPKE 190 200 210 220 230 240 GPYVEDLSKH LVQNYSDVEE LMDAGNINRT TAATGMNDVS SRSHAIFTIK FTQAKFDAEM 250 260 270 280 290 300 PCETVSKIHL VDLAGSERAD ATGATGVRLK EGGNINKSLV TLGNVISALA DLSQDAANPL 310 320 330 340 350 360 VKKKQVFVPY RDSVLTWLLK DSLGGNSKTI MIATISPADV NYGETLSTLR YANRAKNIIN 370 380 390 400 410 420 KPTINEDANV KLIRELRAEI ARLKTLLAQG NQIALLDSPT ALSMEEKLHQ NEARVQELTK 430 440 450 460 470 480 EWTNKWNETQ NILKEQTLAL RKEGIGVVLD SELPHLIGID DDLLSTGIIL YHLKEGQTYV 490 500 510 520 530 540 GREDASTEQD IVLHGLDLES EHCVFENAGG TVTLIPLRGS QCSVNGVQIV DATQLNQGAV 550 560 570 580 590 600 ILLGRTNMFR FNHPKEAAKL REKRKSGLLS SFSLSMTDLS KSCENLSAVM LYNPGLEFER 610 620 630 640 650 660 QQREELEKLE SKRKLIEEME EKQKSDKAEL ERMQQEVETR RKETEIVQRQ IRKQEESLKR 670 680 690 700 710 720 RSFHIENKLK DLLAEKERFE EERLREQQGL EQQRRQEEES LFRIREELRK LQELNSHEQA 730 740 750 760 770 780 EKVQIFQELD RLHQEQNAQS AKLRLEKRRL EEEEKEQVQR VAHLEEQLRK RQDTAPLLCP 790 800 810 820 830 840 GEAQRAQEEK RELESIREAL LQAKEMRAGG DHTCRDELER AQQYFLEFKR RQLVKLASLE 850 860 870 880 890 900 KDLVQQKDLL SKEVQEEKVA LEHVKCDAGG DPSFLATDDG NILGGPPDLD KIKTAETRLQ 910 920 930 940 950 960 SREHQLQDLL QNHLPALLEE KQRVLDALDS GVLGLDTTLC QVEKEVGEKE EQIAQYQANA 970 980 990 1000 1010 1020 SQLQQLRATF EFTANVARQE EKVRRKEKEI LESQEKQQRE ALEQAVAKLE QRRSALQRCS 1030 1040 1050 1060 1070 1080 TLDLEIQEQR QKLGSLHTSE WSGWQASLET DGEALEMDPA RLEHEIHQLK QKICEVDGVQ 1090 1100 1110 1120 1130 1140 RPHHGILEGQ AVLSSLPPSG GNSHLAPLMD ARISAYIEEE VQRRLHDLHR AIGDANHTPA 1150 1160 1170 1180 1190 1200 DVMKSNEELH NGTTQRKLKY ERMYSRSLGT NRDDLKDPIK ISIPRYVLCG QGKDEHFEFE 1210 1220 1230 1240 1250 1260 VKISVLDETW TVFRRYSRFR EMHKTLKLKY AELAALEFPP KKLFGNKDER VVAERRTHLE 1270 1280 1290 1300 1310 KYLREFFSVM LQSETSPLHI NKVGLTLSKH TICEFSPFFK KGVFDYSSHG TG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6J.
[2]	"Identification and classification of 16 new kinesin superfamily (KIF) proteins in mouse genome." Nakagawa T., Tanaka Y., Matsuoka E., Kondo S., Okada Y., Noda Y., Kanai Y., Hirokawa N. Proc. Natl. Acad. Sci. U.S.A. 94:9654-9659(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 101-239. Strain: ICR.
[3]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 195-1312. Strain: C57BL/6J. Tissue: Gonad.
[4]	"Prediction of the coding sequences of mouse homologues of KIAA gene: III. The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries." Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S., Saga Y., Nagase T., Ohara O., Koga H. DNA Res. 10:167-180(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 487-1161. Tissue: Embryonic tail.
[5]	"The phagosomal proteome in interferon-gamma-activated macrophages." Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P. Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1145, MASS SPECTROMETRY. Tissue: Macrophage.
[6]	"KIF16B/Rab14 molecular motor complex is critical for early embryonic development by transporting FGF receptor." Ueno H., Huang X., Tanaka Y., Hirokawa N. Dev. Cell 20:60-71(2011) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, DISRUPTION PHENOTYPE, INTERACTION WITH RAB14.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL731712, AL731790, AL929136 Genomic DNA. Translation: CAM13299.1. AL731790, AL731712, AL929136 Genomic DNA. Translation: CAM13408.1. AL929136, AL731712, AL731790 Genomic DNA. Translation: CAM24500.1. AB001423 mRNA. Translation: BAA22383.1. AK033018 mRNA. Translation: BAC28130.2. AK160835 mRNA. Translation: BAE36039.1. AK129401 Transcribed RNA. Translation: BAC98211.1. Sequence problems.
IPI	IPI00353998.
RefSeq	NP_001074602.1. NM_001081133.2.
UniGene	Mm.251934.
3D structure databases
ProteinModelPortal	B1AVY7.
SMR	B1AVY7. Positions 2-389, 428-563, 1174-1307.
ModBase	Search...
Protein-protein interaction databases
STRING	10090.ENSMUSP00000042551.
Proteomic databases
PaxDb	B1AVY7.
PRIDE	B1AVY7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000043589; ENSMUSP00000042551; ENSMUSG00000038844.
GeneID	16558.
KEGG	mmu:16558.
UCSC	uc008mpz.1. mouse.
Organism-specific databases
CTD	55614.
MGI	MGI:1098240. Kif16b.
Rouge	Search...
Phylogenomic databases
eggNOG	COG5059.
GeneTree	ENSGT00700000104366.
HOGENOM	HOG000044239.
HOVERGEN	HBG099559.
InParanoid	Q3TUD2.
KO	K10392.
OMA	IAERRSH.
OrthoDB	EOG4255RZ.
Gene expression databases
ArrayExpress	B1AVY7.
Bgee	B1AVY7.
Genevestigator	B1AVY7.
Family and domain databases
Gene3D	2.60.200.20. 1 hit. 3.30.1520.10. 1 hit. 3.40.850.10. 1 hit.
InterPro	IPR000253. FHA_dom. IPR019821. Kinesin_motor_CS. IPR001752. Kinesin_motor_dom. IPR001683. Phox. IPR008984. SMAD_FHA_domain. [Graphical view]
Pfam	PF00498. FHA. 1 hit. PF00225. Kinesin. 1 hit. PF00787. PX. 1 hit. [Graphical view]
PRINTS	PR00380. KINESINHEAVY.
SMART	SM00240. FHA. 1 hit. SM00129. KISc. 1 hit. SM00312. PX. 1 hit. [Graphical view]
SUPFAM	SSF64268. PX. 1 hit. SSF49879. SMAD_FHA. 1 hit.
PROSITE	PS50006. FHA_DOMAIN. False negative. PS00411. KINESIN_MOTOR_DOMAIN1. 1 hit. PS50067. KINESIN_MOTOR_DOMAIN2. 1 hit. PS50195. PX. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	290039.
SOURCE	Search...

Entry information

Entry name

KI16B_MOUSE

Accession

Primary (citable) accession number: B1AVY7
Secondary accession number(s): O35056 , Q3TUD2, Q6ZPM0, Q8BZZ9

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 31, 2011
Last sequence update:	April 8, 2008
Last modified:	May 1, 2013
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program