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Protein

Kinesin-like protein KIF16B

Gene

Kif16b

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plus end-directed microtubule-dependent motor protein involved in endosome transport and receptor recycling and degradation. Regulates the plus end motility of early endosomes and the balance between recycling and degradation of receptors such as EGF receptor (EGFR) and FGF receptor (FGFR). Regulates the Golgi to endosome transport of FGFR-containing vesicles during early development, a key process for developing basement membrane and epiblast and primitive endoderm lineages during early postimplantation development.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi102 – 109ATPPROSITE-ProRule annotation8

GO - Molecular functioni

GO - Biological processi

  • cytoskeleton-dependent intracellular transport Source: GO_Central
  • early endosome to late endosome transport Source: UniProtKB
  • endoderm development Source: UniProtKB
  • epidermal growth factor receptor signaling pathway Source: UniProtKB
  • fibroblast growth factor receptor signaling pathway Source: UniProtKB
  • formation of primary germ layer Source: UniProtKB
  • Golgi to endosome transport Source: UniProtKB
  • microtubule-based movement Source: GO_Central
  • receptor catabolic process Source: UniProtKB
  • regulation of receptor recycling Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Motor protein

Keywords - Biological processi

Transport

Keywords - Ligandi

ATP-binding, Lipid-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Kinesin-like protein KIF16B
Gene namesi
Name:Kif16b
Synonyms:Kiaa1590
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:1098240. Kif16b.

Subcellular locationi

  • Cytoplasmcytoskeleton By similarity
  • Early endosome membrane By similarity

  • Note: It is unclear whether association with endosomes is mediated via phosphatidylinositol 3-phosphate (PtdIns3P)-binding or via its interaction with RAB14.

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Endosome, Membrane, Microtubule

Pathology & Biotechi

Disruption phenotypei

Embryonic death. Embryos are arrested at the blastocyst stage: the primitive endoderm and epiblast cannot be distinguished and appear as cell clumps resembling the inner cell mass (ICM) of the blastocyst. Embryos do not develop an epiblast epithelium and the uterine reaction appears to be incomplete. Development of the primitive endoderm and a basement membrane derived from it are severely affected in embryos at 4.5 dpc.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004095001 – 1312Kinesin-like protein KIF16BAdd BLAST1312

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei398PhosphoserineBy similarity1
Modified residuei577PhosphothreonineBy similarity1
Modified residuei582PhosphoserineCombined sources1
Modified residuei838PhosphoserineCombined sources1
Modified residuei1047PhosphoserineBy similarity1
Modified residuei1145PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiB1AVY7.
MaxQBiB1AVY7.
PaxDbiB1AVY7.
PeptideAtlasiB1AVY7.
PRIDEiB1AVY7.

PTM databases

iPTMnetiB1AVY7.
PhosphoSitePlusiB1AVY7.

Expressioni

Gene expression databases

BgeeiENSMUSG00000038844.
GenevisibleiB1AVY7. MM.

Interactioni

Subunit structurei

Interacts with PTPN21 (By similarity). Interacts with RAB14.By similarity1 Publication

GO - Molecular functioni

  • Rab GTPase binding Source: UniProtKB

Protein-protein interaction databases

IntActiB1AVY7. 1 interactor.
MINTiMINT-4113721.
STRINGi10090.ENSMUSP00000042551.

Structurei

3D structure databases

ProteinModelPortaliB1AVY7.
SMRiB1AVY7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini3 – 358Kinesin motorPROSITE-ProRule annotationAdd BLAST356
Domaini?480 – ?544FHAAdd BLAST65
Domaini1177 – 1291PXPROSITE-ProRule annotationAdd BLAST115

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili366 – 425Sequence analysisAdd BLAST60
Coiled coili835 – 913Sequence analysisAdd BLAST79
Coiled coili941 – 1073Sequence analysisAdd BLAST133

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi597 – 798Glu-richAdd BLAST202

Domaini

The PX domain mediates binding to phosphatidylinositol 3-phosphate (PtdIns3P), phosphatidylinositol 3,4-bisphosphate (PtdIns(3,4)P2), phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2) and phosphatidylinositol 3,4,5-trisphosphate (PtdIns(3,4,5)P3). Does not bind phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2) (By similarity).By similarity

Sequence similaritiesi

Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.PROSITE-ProRule annotation
Contains 1 FHA domain.Curated
Contains 1 kinesin motor domain.PROSITE-ProRule annotation
Contains 1 PX (phox homology) domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG0245. Eukaryota.
KOG2101. Eukaryota.
COG5059. LUCA.
GeneTreeiENSGT00840000129680.
HOGENOMiHOG000044239.
HOVERGENiHBG099559.
InParanoidiB1AVY7.
KOiK17916.
OMAiLHIDKVG.
OrthoDBiEOG091G00V7.
PhylomeDBiB1AVY7.
TreeFamiTF105221.

Family and domain databases

Gene3Di2.60.200.20. 1 hit.
3.30.1520.10. 1 hit.
3.40.850.10. 1 hit.
InterProiIPR000253. FHA_dom.
IPR027640. Kinesin-like_fam.
IPR019821. Kinesin_motor_CS.
IPR001752. Kinesin_motor_dom.
IPR027417. P-loop_NTPase.
IPR001683. Phox.
IPR008984. SMAD_FHA_domain.
[Graphical view]
PANTHERiPTHR24115. PTHR24115. 4 hits.
PfamiPF00498. FHA. 1 hit.
PF00225. Kinesin. 1 hit.
PF00787. PX. 1 hit.
[Graphical view]
PRINTSiPR00380. KINESINHEAVY.
SMARTiSM00129. KISc. 1 hit.
SM00312. PX. 1 hit.
[Graphical view]
SUPFAMiSSF49879. SSF49879. 1 hit.
SSF52540. SSF52540. 1 hit.
SSF64268. SSF64268. 1 hit.
PROSITEiPS00411. KINESIN_MOTOR_1. 1 hit.
PS50067. KINESIN_MOTOR_2. 1 hit.
PS50195. PX. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B1AVY7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASVKVAVRV RPMNRREKDL EAKFIIQMEK SKTTITNLKI PEGGTGDSGR
60 70 80 90 100
ERTKTFTYDF SFYSADTKSP DYVSQEMVFK TLGTDVVKSA FEGYNACVFA
110 120 130 140 150
YGQTGSGKSY TMMGNSGDSG LIPRICEALF SRINETTRWD EASFRTEVSY
160 170 180 190 200
LEIYNERVRD LLRRKSSKTF NLRVREHPKE GPYVEDLSKH LVQNYSDVEE
210 220 230 240 250
LMDAGNINRT TAATGMNDVS SRSHAIFTIK FTQAKFDAEM PCETVSKIHL
260 270 280 290 300
VDLAGSERAD ATGATGVRLK EGGNINKSLV TLGNVISALA DLSQDAANPL
310 320 330 340 350
VKKKQVFVPY RDSVLTWLLK DSLGGNSKTI MIATISPADV NYGETLSTLR
360 370 380 390 400
YANRAKNIIN KPTINEDANV KLIRELRAEI ARLKTLLAQG NQIALLDSPT
410 420 430 440 450
ALSMEEKLHQ NEARVQELTK EWTNKWNETQ NILKEQTLAL RKEGIGVVLD
460 470 480 490 500
SELPHLIGID DDLLSTGIIL YHLKEGQTYV GREDASTEQD IVLHGLDLES
510 520 530 540 550
EHCVFENAGG TVTLIPLRGS QCSVNGVQIV DATQLNQGAV ILLGRTNMFR
560 570 580 590 600
FNHPKEAAKL REKRKSGLLS SFSLSMTDLS KSCENLSAVM LYNPGLEFER
610 620 630 640 650
QQREELEKLE SKRKLIEEME EKQKSDKAEL ERMQQEVETR RKETEIVQRQ
660 670 680 690 700
IRKQEESLKR RSFHIENKLK DLLAEKERFE EERLREQQGL EQQRRQEEES
710 720 730 740 750
LFRIREELRK LQELNSHEQA EKVQIFQELD RLHQEQNAQS AKLRLEKRRL
760 770 780 790 800
EEEEKEQVQR VAHLEEQLRK RQDTAPLLCP GEAQRAQEEK RELESIREAL
810 820 830 840 850
LQAKEMRAGG DHTCRDELER AQQYFLEFKR RQLVKLASLE KDLVQQKDLL
860 870 880 890 900
SKEVQEEKVA LEHVKCDAGG DPSFLATDDG NILGGPPDLD KIKTAETRLQ
910 920 930 940 950
SREHQLQDLL QNHLPALLEE KQRVLDALDS GVLGLDTTLC QVEKEVGEKE
960 970 980 990 1000
EQIAQYQANA SQLQQLRATF EFTANVARQE EKVRRKEKEI LESQEKQQRE
1010 1020 1030 1040 1050
ALEQAVAKLE QRRSALQRCS TLDLEIQEQR QKLGSLHTSE WSGWQASLET
1060 1070 1080 1090 1100
DGEALEMDPA RLEHEIHQLK QKICEVDGVQ RPHHGILEGQ AVLSSLPPSG
1110 1120 1130 1140 1150
GNSHLAPLMD ARISAYIEEE VQRRLHDLHR AIGDANHTPA DVMKSNEELH
1160 1170 1180 1190 1200
NGTTQRKLKY ERMYSRSLGT NRDDLKDPIK ISIPRYVLCG QGKDEHFEFE
1210 1220 1230 1240 1250
VKISVLDETW TVFRRYSRFR EMHKTLKLKY AELAALEFPP KKLFGNKDER
1260 1270 1280 1290 1300
VVAERRTHLE KYLREFFSVM LQSETSPLHI NKVGLTLSKH TICEFSPFFK
1310
KGVFDYSSHG TG
Length:1,312
Mass (Da):150,058
Last modified:April 8, 2008 - v1
Checksum:i4397E49FF4E93881
GO

Sequence cautioni

The sequence BAC98211 differs from that shown. Partially unspliced pre-RNA.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL731712, AL731790, AL929136 Genomic DNA. Translation: CAM13299.1.
AL731790, AL731712, AL929136 Genomic DNA. Translation: CAM13408.1.
AL929136, AL731712, AL731790 Genomic DNA. Translation: CAM24500.1.
AB001423 mRNA. Translation: BAA22383.1.
AK033018 mRNA. Translation: BAC28130.2.
AK160835 mRNA. Translation: BAE36039.1.
AK129401 Transcribed RNA. Translation: BAC98211.1. Sequence problems.
CCDSiCCDS38251.1.
RefSeqiNP_001074602.1. NM_001081133.2.
UniGeneiMm.251934.

Genome annotation databases

EnsembliENSMUST00000043589; ENSMUSP00000042551; ENSMUSG00000038844.
GeneIDi16558.
KEGGimmu:16558.
UCSCiuc008mpz.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL731712, AL731790, AL929136 Genomic DNA. Translation: CAM13299.1.
AL731790, AL731712, AL929136 Genomic DNA. Translation: CAM13408.1.
AL929136, AL731712, AL731790 Genomic DNA. Translation: CAM24500.1.
AB001423 mRNA. Translation: BAA22383.1.
AK033018 mRNA. Translation: BAC28130.2.
AK160835 mRNA. Translation: BAE36039.1.
AK129401 Transcribed RNA. Translation: BAC98211.1. Sequence problems.
CCDSiCCDS38251.1.
RefSeqiNP_001074602.1. NM_001081133.2.
UniGeneiMm.251934.

3D structure databases

ProteinModelPortaliB1AVY7.
SMRiB1AVY7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiB1AVY7. 1 interactor.
MINTiMINT-4113721.
STRINGi10090.ENSMUSP00000042551.

PTM databases

iPTMnetiB1AVY7.
PhosphoSitePlusiB1AVY7.

Proteomic databases

EPDiB1AVY7.
MaxQBiB1AVY7.
PaxDbiB1AVY7.
PeptideAtlasiB1AVY7.
PRIDEiB1AVY7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000043589; ENSMUSP00000042551; ENSMUSG00000038844.
GeneIDi16558.
KEGGimmu:16558.
UCSCiuc008mpz.1. mouse.

Organism-specific databases

CTDi55614.
MGIiMGI:1098240. Kif16b.
RougeiSearch...

Phylogenomic databases

eggNOGiKOG0245. Eukaryota.
KOG2101. Eukaryota.
COG5059. LUCA.
GeneTreeiENSGT00840000129680.
HOGENOMiHOG000044239.
HOVERGENiHBG099559.
InParanoidiB1AVY7.
KOiK17916.
OMAiLHIDKVG.
OrthoDBiEOG091G00V7.
PhylomeDBiB1AVY7.
TreeFamiTF105221.

Miscellaneous databases

PROiB1AVY7.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000038844.
GenevisibleiB1AVY7. MM.

Family and domain databases

Gene3Di2.60.200.20. 1 hit.
3.30.1520.10. 1 hit.
3.40.850.10. 1 hit.
InterProiIPR000253. FHA_dom.
IPR027640. Kinesin-like_fam.
IPR019821. Kinesin_motor_CS.
IPR001752. Kinesin_motor_dom.
IPR027417. P-loop_NTPase.
IPR001683. Phox.
IPR008984. SMAD_FHA_domain.
[Graphical view]
PANTHERiPTHR24115. PTHR24115. 4 hits.
PfamiPF00498. FHA. 1 hit.
PF00225. Kinesin. 1 hit.
PF00787. PX. 1 hit.
[Graphical view]
PRINTSiPR00380. KINESINHEAVY.
SMARTiSM00129. KISc. 1 hit.
SM00312. PX. 1 hit.
[Graphical view]
SUPFAMiSSF49879. SSF49879. 1 hit.
SSF52540. SSF52540. 1 hit.
SSF64268. SSF64268. 1 hit.
PROSITEiPS00411. KINESIN_MOTOR_1. 1 hit.
PS50067. KINESIN_MOTOR_2. 1 hit.
PS50195. PX. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiKI16B_MOUSE
AccessioniPrimary (citable) accession number: B1AVY7
Secondary accession number(s): O35056
, Q3TUD2, Q6ZPM0, Q8BZZ9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 31, 2011
Last sequence update: April 8, 2008
Last modified: November 2, 2016
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.