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Protein

Receptor-type tyrosine-protein phosphatase U

Gene

Ptpru

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Tyrosine-protein phosphatase which dephosphorylates CTNNB1. Regulates CTNNB1 function both in cell adhesion and signaling. May function in cell proliferation and migration and play a role in the maintenance of epithelial integrity. May play a role in megakaryocytopoiesis (By similarity).By similarity

Catalytic activityi

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei1053SubstrateSequence analysis1
Active sitei1085Phosphocysteine intermediateBy similarity1
Binding sitei1129SubstrateBy similarity1
Active sitei1380Phosphocysteine intermediateBy similarity1

GO - Molecular functioni

  • beta-catenin binding Source: MGI
  • protein tyrosine phosphatase activity Source: MGI

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase, Receptor

Keywords - Biological processi

Cell adhesion, Differentiation

Enzyme and pathway databases

ReactomeiR-MMU-1433557. Signaling by SCF-KIT.

Names & Taxonomyi

Protein namesi
Recommended name:
Receptor-type tyrosine-protein phosphatase U (EC:3.1.3.48)
Short name:
R-PTP-U
Alternative name(s):
Ftp-1
Pancreatic carcinoma phosphatase 2
Short name:
PCP-2
Protein-tyrosine phosphatase-lamda
Short name:
PTP-lambda
Short name:
PTPlambda
Receptor-type protein-tyrosine phosphatase psi
Short name:
R-PTP-psi
Gene namesi
Name:Ptpru
Synonyms:Pcp2, Ptpf
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:1321151. Ptpru.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini19 – 749ExtracellularSequence analysisAdd BLAST731
Transmembranei750 – 770HelicalSequence analysisAdd BLAST21
Topological domaini771 – 1446CytoplasmicSequence analysisAdd BLAST676

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 18Sequence analysisAdd BLAST18
ChainiPRO_000037165919 – 1446Receptor-type tyrosine-protein phosphatase UAdd BLAST1428

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi75N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi210 ↔ 264Sequence analysis
Glycosylationi410N-linked (GlcNAc...)Sequence analysis1
Glycosylationi685N-linked (GlcNAc...)Sequence analysis1
Modified residuei848PhosphoserineBy similarity1
Modified residuei853PhosphoserineCombined sources1
Modified residuei863PhosphoserineCombined sources1
Modified residuei865PhosphotyrosineCombined sources1

Post-translational modificationi

The extracellular domain is proteolytically processed through cleavage within the fibronectin type-III 4 domain. In addition to the 190 kDa full-length protein, proteolytic products of 100 kDa, 80 kDa and 73 kDa are observed (By similarity).By similarity
N-glycosylated.By similarity
Phosphorylated on tyrosine residues upon activation of KIT with stem cell factor (SCF). The 73 kDa proteolytic product is not phosphorylated (By similarity).By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiB1AUH1.
PaxDbiB1AUH1.
PRIDEiB1AUH1.

PTM databases

iPTMnetiB1AUH1.
PhosphoSitePlusiB1AUH1.

Expressioni

Tissue specificityi

Transcripts of different sizes are differentially expressed in a subset of tissues. Detected in brain, lung, skeletal muscle, heart, kidney and placenta. In brain; expressed in olfactory bulb, cerebral cortex, hippocampus and cerebellum.3 Publications

Developmental stagei

Expressed throughout embryonic development. First detected at E7.1 Publication

Gene expression databases

BgeeiENSMUSG00000028909.
ExpressionAtlasiB1AUH1. baseline and differential.
GenevisibleiB1AUH1. MM.

Interactioni

Subunit structurei

Forms homooligomeric complexes which mediate cell homotypic adhesion (Probable). Interacts (via the cytoplasmic juxtamembrane domain) with CTNNB1; may mediate interaction with the cadherin/catenin adhesion complex. Interacts with KIT (By similarity). May interact with AP3B1 (By similarity).By similarityCurated

GO - Molecular functioni

  • beta-catenin binding Source: MGI

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000030741.

Structurei

3D structure databases

ProteinModelPortaliB1AUH1.
SMRiB1AUH1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini25 – 188MAMPROSITE-ProRule annotationAdd BLAST164
Domaini190 – 275Ig-like C2-typeAdd BLAST86
Domaini288 – 383Fibronectin type-III 1PROSITE-ProRule annotationAdd BLAST96
Domaini386 – 484Fibronectin type-III 2PROSITE-ProRule annotationAdd BLAST99
Domaini485 – 591Fibronectin type-III 3PROSITE-ProRule annotationAdd BLAST107
Domaini592 – 668Fibronectin type-III 4PROSITE-ProRule annotationAdd BLAST77
Domaini888 – 1144Tyrosine-protein phosphatase 1PROSITE-ProRule annotationAdd BLAST257
Domaini1176 – 1439Tyrosine-protein phosphatase 2PROSITE-ProRule annotationAdd BLAST264

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni771 – 887Mediates interaction with CTNNB11 PublicationAdd BLAST117
Regioni1085 – 1091Substrate bindingBy similarity7

Sequence similaritiesi

Contains 4 fibronectin type-III domains.PROSITE-ProRule annotation
Contains 1 MAM domain.PROSITE-ProRule annotation
Contains 2 tyrosine-protein phosphatase domains.PROSITE-ProRule annotation

Keywords - Domaini

Immunoglobulin domain, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG4228. Eukaryota.
COG5599. LUCA.
GeneTreeiENSGT00760000118900.
HOGENOMiHOG000049029.
InParanoidiB1AUH1.
KOiK16662.
OMAiASFQCMA.
OrthoDBiEOG091G00CK.
PhylomeDBiB1AUH1.
TreeFamiTF312900.

Family and domain databases

CDDicd00063. FN3. 3 hits.
cd06263. MAM. 1 hit.
Gene3Di2.60.40.10. 4 hits.
3.90.190.10. 2 hits.
InterProiIPR013320. ConA-like_dom.
IPR003961. FN3_dom.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
IPR000998. MAM_dom.
IPR029021. Prot-tyrosine_phosphatase-like.
IPR000242. PTPase_domain.
IPR016130. Tyr_Pase_AS.
IPR003595. Tyr_Pase_cat.
IPR000387. TYR_PHOSPHATASE_dom.
[Graphical view]
PfamiPF00041. fn3. 2 hits.
PF00629. MAM. 1 hit.
PF00102. Y_phosphatase. 2 hits.
[Graphical view]
PRINTSiPR00020. MAMDOMAIN.
PR00700. PRTYPHPHTASE.
SMARTiSM00060. FN3. 3 hits.
SM00409. IG. 1 hit.
SM00137. MAM. 1 hit.
SM00194. PTPc. 2 hits.
SM00404. PTPc_motif. 2 hits.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 1 hit.
SSF49265. SSF49265. 2 hits.
SSF49899. SSF49899. 1 hit.
SSF52799. SSF52799. 2 hits.
PROSITEiPS50853. FN3. 3 hits.
PS50060. MAM_2. 1 hit.
PS00383. TYR_PHOSPHATASE_1. 2 hits.
PS50056. TYR_PHOSPHATASE_2. 2 hits.
PS50055. TYR_PHOSPHATASE_PTP. 2 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: B1AUH1-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MARAQALVLA LTFQFCAPET ETPAAGCTFE EASDPVVPCE FSQAQYDDFQ
60 70 80 90 100
WEQVRIHPGT RTPEDLPHGA YLMVNASQHA PGQRAHIIFQ TLSENDTHCV
110 120 130 140 150
QFSYFLYSRD GHSPGTLGVY VRVNGGPLGS AVWNMTGSHG RQWHQAELAV
160 170 180 190 200
STFWPNEYQV LFEALISPDH KGYIGLDDIL LFSYPCAKAP HFSRLGDVEV
210 220 230 240 250
NAGQNASFQC MAAGRAAEAE HFFLQRQSGV LVPAAGVRHI SHRRFLATFP
260 270 280 290 300
LASVGRSEQD LYRCVSQAPR GAGVSNFAEL IVKEPPTPIA PPQLLRAGPT
310 320 330 340 350
YLIIQLNTNS IIGDGPIVRK EIEYRMARGP WAEVHAVNLQ TYKLWHLDPD
360 370 380 390 400
TEYEISVLLT RPGDGGTGRP GPPLISRTKC AEPTRAPKGL AFAEIQARQL
410 420 430 440 450
TLQWEPLGYN VTRCHTYAVS LCYRYTLGGS HNQTIRECVK MERGASRYTI
460 470 480 490 500
KNLLPFRNIH VRLILTNPEG RKEGKEVTFQ TDEDVPGGIA AESLTFTPLE
510 520 530 540 550
DMIFLKWEEP QEPNGLITQY EISYQSIESS DPAVNVPGPR RTISKLRNET
560 570 580 590 600
YHVFSNLHPG TTYLFSVRAR TSKGFGQAAL TEITTNISAP SFDYADMPSP
610 620 630 640 650
LGESENTITV LLRPAQGRGA PISVYQVVVE EERPRRLRRE PGAQDCFSVP
660 670 680 690 700
LTFETALARG LVHYFGAELA ASSLLEAMPF TVGDNQTYRG FWNPPLEPRK
710 720 730 740 750
AYLIYFQAAS HLKGETRLNC IRIARKAACK ESKRPLEVSQ RSEEMGLILG
760 770 780 790 800
ICAGGLAVLI LLLGAIIVII RKGRDRYAYS YYPKPVNMTK ATVNYRQEKT
810 820 830 840 850
HMMSAVDRSF TDQSTLQEDE RLGLSFMDAP GYSPRGDQRS GGVTEASSLL
860 870 880 890 900
GGSPRRPCGR KGSPYHTGQL HPAVRVADLL QHINQMKTAE GYGFKQEYES
910 920 930 940 950
FFEGWDATKK KDKLKGGRQE PVSAYDRHHV KLHPMLADPD ADYISANYID
960 970 980 990 1000
GYHRSNHFIA TQGPKPEMIY DFWRMVWQEQ CASIVMITKL VEVGRVKCSR
1010 1020 1030 1040 1050
YWPEDSDMYG DIKITLVKTE TLAEYVVRTF ALERRGYSAR HEVRQFHFTA
1060 1070 1080 1090 1100
WPEHGVPYHA TGLLAFIRRV KASTPPDAGP IVIHCSAGTG RTGCYIVLDV
1110 1120 1130 1140 1150
MLDMAECEGV VDIYNCVKTL CSRRVNMIQT EEQYIFIHDA ILEACLCGET
1160 1170 1180 1190 1200
TIPVNEFKAT YREMIRIDPQ SNSSQLREEF QTLNSVTPPL DVEECSIALL
1210 1220 1230 1240 1250
PRNRDKNRSM DVLPPDRCLP FLISSDGDPN NYINAALTDS YTRSAAFIVT
1260 1270 1280 1290 1300
LHPLQSTTPD FWRLVYDYGC TSIVMLNQLN QSNSAWPCLQ YWPEPGRQQY
1310 1320 1330 1340 1350
GLMEVEFVSG TANEDLVSRV FRVQNSSRLQ EGHLLVRHFQ FLRWSAYRDT
1360 1370 1380 1390 1400
PDSRKAFLHL LAEVDKWQAE SGDGRTVVHC LNGGGRSGTF CACATVLEMI
1410 1420 1430 1440
RCHSLVDVFF AAKTLRNYKP NMVETMDQYH FCYDVALEYL EALELR
Length:1,446
Mass (Da):162,494
Last modified:April 8, 2008 - v1
Checksum:iCBF639D8150D0D0B
GO
Isoform 2 (identifier: B1AUH1-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     774-783: Missing.

Show »
Length:1,436
Mass (Da):161,158
Checksum:i8D406DE4948643FF
GO

Sequence cautioni

The sequence AAH80736 differs from that shown. Contaminating sequence. Potential poly-A sequence.Curated
The sequence AAH80736 differs from that shown. Reason: Erroneous termination at position 618. Translated as Arg.Curated
The sequence BAE43351 differs from that shown. Intron retention.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti80A → T in AAB17895 (PubMed:9054423).Curated1
Sequence conflicti158Y → F in AAB17895 (PubMed:9054423).Curated1
Sequence conflicti265V → A in AAH80736 (PubMed:15489334).Curated1
Sequence conflicti387P → S in AAH80736 (PubMed:15489334).Curated1
Sequence conflicti506K → R in AAH80736 (PubMed:15489334).Curated1
Sequence conflicti1158K → R in AAB17895 (PubMed:9054423).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_037086774 – 783Missing in isoform 2. 4 Publications10

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D88187 mRNA. Translation: BAA23475.1.
U55057 mRNA. Translation: AAB17895.1.
AL670959 Genomic DNA. Translation: CAM27391.1.
AL670959 Genomic DNA. Translation: CAM27392.1.
CH466552 Genomic DNA. Translation: EDL30128.1.
AK052720 mRNA. Translation: BAE43351.1. Sequence problems.
BC080736 mRNA. Translation: AAH80736.1. Sequence problems.
CCDSiCCDS18714.1. [B1AUH1-2]
CCDS38896.1. [B1AUH1-1]
RefSeqiNP_001076588.1. NM_001083119.2. [B1AUH1-1]
NP_035344.2. NM_011214.2. [B1AUH1-2]
UniGeneiMm.4860.

Genome annotation databases

EnsembliENSMUST00000030741; ENSMUSP00000030741; ENSMUSG00000028909. [B1AUH1-1]
ENSMUST00000105987; ENSMUSP00000101607; ENSMUSG00000028909. [B1AUH1-2]
GeneIDi19273.
KEGGimmu:19273.
UCSCiuc008vaa.1. mouse. [B1AUH1-1]
uc008vab.1. mouse. [B1AUH1-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D88187 mRNA. Translation: BAA23475.1.
U55057 mRNA. Translation: AAB17895.1.
AL670959 Genomic DNA. Translation: CAM27391.1.
AL670959 Genomic DNA. Translation: CAM27392.1.
CH466552 Genomic DNA. Translation: EDL30128.1.
AK052720 mRNA. Translation: BAE43351.1. Sequence problems.
BC080736 mRNA. Translation: AAH80736.1. Sequence problems.
CCDSiCCDS18714.1. [B1AUH1-2]
CCDS38896.1. [B1AUH1-1]
RefSeqiNP_001076588.1. NM_001083119.2. [B1AUH1-1]
NP_035344.2. NM_011214.2. [B1AUH1-2]
UniGeneiMm.4860.

3D structure databases

ProteinModelPortaliB1AUH1.
SMRiB1AUH1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000030741.

PTM databases

iPTMnetiB1AUH1.
PhosphoSitePlusiB1AUH1.

Proteomic databases

MaxQBiB1AUH1.
PaxDbiB1AUH1.
PRIDEiB1AUH1.

Protocols and materials databases

DNASUi19273.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000030741; ENSMUSP00000030741; ENSMUSG00000028909. [B1AUH1-1]
ENSMUST00000105987; ENSMUSP00000101607; ENSMUSG00000028909. [B1AUH1-2]
GeneIDi19273.
KEGGimmu:19273.
UCSCiuc008vaa.1. mouse. [B1AUH1-1]
uc008vab.1. mouse. [B1AUH1-2]

Organism-specific databases

CTDi10076.
MGIiMGI:1321151. Ptpru.

Phylogenomic databases

eggNOGiKOG4228. Eukaryota.
COG5599. LUCA.
GeneTreeiENSGT00760000118900.
HOGENOMiHOG000049029.
InParanoidiB1AUH1.
KOiK16662.
OMAiASFQCMA.
OrthoDBiEOG091G00CK.
PhylomeDBiB1AUH1.
TreeFamiTF312900.

Enzyme and pathway databases

ReactomeiR-MMU-1433557. Signaling by SCF-KIT.

Miscellaneous databases

PROiB1AUH1.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000028909.
ExpressionAtlasiB1AUH1. baseline and differential.
GenevisibleiB1AUH1. MM.

Family and domain databases

CDDicd00063. FN3. 3 hits.
cd06263. MAM. 1 hit.
Gene3Di2.60.40.10. 4 hits.
3.90.190.10. 2 hits.
InterProiIPR013320. ConA-like_dom.
IPR003961. FN3_dom.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
IPR000998. MAM_dom.
IPR029021. Prot-tyrosine_phosphatase-like.
IPR000242. PTPase_domain.
IPR016130. Tyr_Pase_AS.
IPR003595. Tyr_Pase_cat.
IPR000387. TYR_PHOSPHATASE_dom.
[Graphical view]
PfamiPF00041. fn3. 2 hits.
PF00629. MAM. 1 hit.
PF00102. Y_phosphatase. 2 hits.
[Graphical view]
PRINTSiPR00020. MAMDOMAIN.
PR00700. PRTYPHPHTASE.
SMARTiSM00060. FN3. 3 hits.
SM00409. IG. 1 hit.
SM00137. MAM. 1 hit.
SM00194. PTPc. 2 hits.
SM00404. PTPc_motif. 2 hits.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 1 hit.
SSF49265. SSF49265. 2 hits.
SSF49899. SSF49899. 1 hit.
SSF52799. SSF52799. 2 hits.
PROSITEiPS50853. FN3. 3 hits.
PS50060. MAM_2. 1 hit.
PS00383. TYR_PHOSPHATASE_1. 2 hits.
PS50056. TYR_PHOSPHATASE_2. 2 hits.
PS50055. TYR_PHOSPHATASE_PTP. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPTPRU_MOUSE
AccessioniPrimary (citable) accession number: B1AUH1
Secondary accession number(s): O35564
, P70125, Q3V312, Q66JV9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: April 8, 2008
Last modified: November 30, 2016
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.