Reviewed, UniProtKB/Swiss-Prot B1AS42 (NB5R5_MOUSE)

Names and origin

Protein names

Recommended name:
    NADH-cytochrome b5 reductase-like
    EC=1.6.2.2

Gene names

Name:

Cyb5rl

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	316 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	NADH-cytochrome b5 reductases are involved in desaturation and elongation of fatty acids, cholesterol biosynthesis, drug metabolism, and, in erythrocyte, methemoglobin reduction By similarity.
Catalytic activity	NADH + 2 ferricytochrome b5 = NAD+ + H+ + 2 ferrocytochrome b5.
Cofactor	FAD By similarity.
Sequence similarities	Belongs to the flavoprotein pyridine nucleotide cytochrome reductase family. Contains 1 FAD-binding FR-type domain.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing
Ligand	FAD Flavoprotein NAD
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	cytochrome-b5 reductase activity Inferred from electronic annotation. Source: EC electron carrier activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: B1AS42-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: B1AS42-2)

The sequence of this isoform differs from the canonical sequence as follows:
     146-180: Missing.

Note: No experimental confirmation available.

Isoform 3 (identifier: B1AS42-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-60: Missing.
     61-67: KQPPESQ → MNNKDLE

Note: No experimental confirmation available.

Isoform 4 (identifier: B1AS42-4)

The sequence of this isoform differs from the canonical sequence as follows:
     1-85: Missing.
     250-283: EVSPEQLPWSYRDKTHFGRLGQELVAELVACCRR → ASPSPSYRGGSRLTEVLDQGGARTGPVCLTLLSL
     284-316: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 316	316	NADH-cytochrome b5 reductase-like		PRO_0000337042
Regions
	Domain	76 – 178	103	FAD-binding FR-type
	Nucleotide binding	158 – 173	16	FAD By similarity
	Nucleotide binding	183 – 215	33	FAD By similarity
Natural variations
	Alternative sequence	1 – 85	85	Missing in isoform 4.		VSP_033848
	Alternative sequence	1 – 60	60	Missing in isoform 3.		VSP_033849
	Alternative sequence	61 – 67	7	KQPPESQ → MNNKDLE in isoform 3.		VSP_033850
	Alternative sequence	146 – 180	35	Missing in isoform 2.		VSP_033851
	Alternative sequence	250 – 283	34	EVSPE…ACCRR → ASPSPSYRGGSRLTEVLDQG GARTGPVCLTLLSL in isoform 4.		VSP_033852
	Alternative sequence	284 – 316	33	Missing in isoform 4.		VSP_033853

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified April 8, 2008. Version 1. Checksum: A5D49CF710DD7AFA	FASTA	316	35,884
	10 20 30 40 50 60 MAETEEEEDS EAWLRLKPVE PLPSQCCGSG CSPCVFDLYY RDLERWETAR ARNDRSLLSG 70 80 90 100 110 120 KQPPESQSCS AKLSPETFLA FHISTMEKVT KDTYLVRFTL PGNSRLGLRP GQHLILRGVV 130 140 150 160 170 180 DGLEIQRAYT PISPVTAEGY FDVLIKCYRT GLMSQYVESW RTGDTAFWRG PFGSFLYEPK 190 200 210 220 230 240 KYGELLMLAA GTGLAPMVPI LQSITDDEDD ETFVTLVGCF KTFEGIYLKT FFQEQARFWN 250 260 270 280 290 300 VQTFFVLSQE VSPEQLPWSY RDKTHFGRLG QELVAELVAC CRRKPFTLVC GSPAFNEDMA 310 RCLLSAGLTE DSYFLF
	Isoform 2. Checksum: 9E1850FEB80E41E1	FASTA	281	31,736
	10 20 30 40 50 60 MAETEEEEDS EAWLRLKPVE PLPSQCCGSG CSPCVFDLYY RDLERWETAR ARNDRSLLSG 70 80 90 100 110 120 KQPPESQSCS AKLSPETFLA FHISTMEKVT KDTYLVRFTL PGNSRLGLRP GQHLILRGVV 130 140 150 160 170 180 DGLEIQRAYT PISPVTAEGY FDVLIKYGEL LMLAAGTGLA PMVPILQSIT DDEDDETFVT 190 200 210 220 230 240 LVGCFKTFEG IYLKTFFQEQ ARFWNVQTFF VLSQEVSPEQ LPWSYRDKTH FGRLGQELVA 250 260 270 280 ELVACCRRKP FTLVCGSPAF NEDMARCLLS AGLTEDSYFL F
	Isoform 3. Checksum: F1339A5F5ED57616	FASTA	256	29,055
	10 20 30 40 50 60 MNNKDLESCS AKLSPETFLA FHISTMEKVT KDTYLVRFTL PGNSRLGLRP GQHLILRGVV 70 80 90 100 110 120 DGLEIQRAYT PISPVTAEGY FDVLIKCYRT GLMSQYVESW RTGDTAFWRG PFGSFLYEPK 130 140 150 160 170 180 KYGELLMLAA GTGLAPMVPI LQSITDDEDD ETFVTLVGCF KTFEGIYLKT FFQEQARFWN 190 200 210 220 230 240 VQTFFVLSQE VSPEQLPWSY RDKTHFGRLG QELVAELVAC CRRKPFTLVC GSPAFNEDMA 250 RCLLSAGLTE DSYFLF
	Isoform 4. Checksum: 4D2A4603BE716A70	FASTA	198	22,148
	10 20 30 40 50 60 MEKVTKDTYL VRFTLPGNSR LGLRPGQHLI LRGVVDGLEI QRAYTPISPV TAEGYFDVLI 70 80 90 100 110 120 KCYRTGLMSQ YVESWRTGDT AFWRGPFGSF LYEPKKYGEL LMLAAGTGLA PMVPILQSIT 130 140 150 160 170 180 DDEDDETFVT LVGCFKTFEG IYLKTFFQEQ ARFWNVQTFF VLSQASPSPS YRGGSRLTEV 190 LDQGGARTGP VCLTLLSL

References

[1]	"Prediction of the coding sequences of mouse homologues of FLJ genes: the complete nucleotide sequences of 110 mouse FLJ-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries." Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S., Saga Y., Kitamura H., Nakagawa T., Nagase T., Ohara O., Koga H. DNA Res. 11:127-135(2004) [PubMed: 15449545] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 4). Strain: ICR. Tissue: Embryonic tail.
[2]	The mouse genome sequencing consortium Submitted (FEB-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6J.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 3). Strain: FVB/N. Tissue: Liver and Mammary tumor.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AK220154 mRNA. Translation: BAD90340.1. Different initiation. AL607132 Genomic DNA. Translation: CAM46300.2. AL607132 Genomic DNA. Translation: CAM46301.1. BC004750 mRNA. Translation: AAH04750.1. Different initiation. BC043687 mRNA. Translation: AAH43687.1.
IPI	IPI00320088. IPI00403588. IPI00895275. IPI00895324.
RefSeq	NP_780680.1.
UniGene	Mm.44608
3D structure databases
ModBase	Search...
Genome annotation databases
Ensembl	ENSMUSG00000028621. Mus musculus. [Contig view]
GeneID	230582.
KEGG	mmu:230582.
Organism-specific databases
MGI	MGI:1919657. Cyb5rl.
Phylogenomic databases
OMA	B1AS42. ECERILQ.
Gene expression databases
Bgee	B1AS42.
Family and domain databases
InterPro	IPR017927. Fd_Rdtase_FAD-bd. IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase. IPR001834. NADH-Cyt_B5_reductase. IPR008333. OxRdtase_FAD-bd. IPR001433. OxRdtase_FAD/NAD_bd. [Graphical view]
Pfam	PF00970. FAD_binding_6. 1 hit. PF00175. NAD_binding_1. 1 hit. [Graphical view]
PRINTS	PR00406. CYTB5RDTASE. PR00371. FPNCR.
PROSITE	PS51384. FAD_FR. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	379943.
SOURCE	Search...

Entry information

Entry name

NB5R5_MOUSE

Accession

Primary (citable) accession number: B1AS42
Secondary accession number(s): B1AS41 , Q571N5, Q80W48, Q99KB7

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	April 8, 2008
Last modified:	July 7, 2009
This is version 14 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)