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Protein

Ubiquitin carboxyl-terminal hydrolase 36

Gene

Usp36

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

May be required for maintaining multiple types of adult stem cells. May function as a transcriptional repressor by continually deubiquiting histone H2B at the promoters of genes critical for cellular differentiation, thereby preventing histone H3 'Lys-4' trimethylation (H3K4) (By similarity).By similarity

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei131 – 1311NucleophilePROSITE-ProRule annotation
Active sitei382 – 3821Proton acceptorPROSITE-ProRule annotation

GO - Molecular functioni

  1. cysteine-type endopeptidase activity Source: GO_Central
  2. poly(A) RNA binding Source: MGI
  3. ubiquitin-specific protease activity Source: MGI

GO - Biological processi

  1. proteasome-mediated ubiquitin-dependent protein catabolic process Source: GO_Central
  2. protein deubiquitination Source: MGI
  3. regulation of proteasomal protein catabolic process Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Ubl conjugation pathway

Protein family/group databases

MEROPSiC19.042.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin carboxyl-terminal hydrolase 36 (EC:3.4.19.12)
Alternative name(s):
Deubiquitinating enzyme 36
Ubiquitin thioesterase 36
Ubiquitin-specific-processing protease 36
Gene namesi
Name:Usp36
Synonyms:Kiaa1453
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:1919594. Usp36.

Subcellular locationi

  1. Nucleusnucleolus By similarity

GO - Cellular componenti

  1. nucleolus Source: MGI
  2. nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10981098Ubiquitin carboxyl-terminal hydrolase 36PRO_0000378494Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei463 – 4631PhosphoserineBy similarity
Modified residuei578 – 5781PhosphoserineBy similarity
Modified residuei663 – 6631PhosphoserineBy similarity
Modified residuei678 – 6781PhosphoserineBy similarity
Modified residuei709 – 7091PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiB1AQJ2.
PaxDbiB1AQJ2.
PRIDEiB1AQJ2.

Expressioni

Gene expression databases

BgeeiB1AQJ2.
ExpressionAtlasiB1AQJ2. baseline and differential.
GenevestigatoriB1AQJ2.

Interactioni

Subunit structurei

Interacts with PAF1.By similarity

Structurei

3D structure databases

ProteinModelPortaliB1AQJ2.
SMRiB1AQJ2. Positions 122-420.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini122 – 423302USPAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase C19 family.Curated
Contains 1 USP domain.Curated

Phylogenomic databases

eggNOGiCOG5533.
GeneTreeiENSGT00780000121889.
HOGENOMiHOG000154759.
InParanoidiB1AQJ2.
KOiK11855.
OMAiHQDPPWH.
OrthoDBiEOG72VH5K.
PhylomeDBiB1AQJ2.
TreeFamiTF315281.

Family and domain databases

InterProiIPR001394. Peptidase_C19_UCH.
IPR018200. USP_CS.
IPR028889. USP_dom.
[Graphical view]
PfamiPF00443. UCH. 1 hit.
[Graphical view]
PROSITEiPS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B1AQJ2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPIVDKLKEA LKPGRKDSAE DGDLGRLLAA SAKKVLLQRI EFEPASKSFS
60 70 80 90 100
YQLESLKSKY VLLSARAEGA SRHRSGDELQ ARKPGTERVS GSGGDGVPAP
110 120 130 140 150
QKVLFPVERL SLRWERVFRV GAGLHNLGNT CFLNSTIQCL TYTPPLANYL
160 170 180 190 200
LSKEHARSCH QGGFCMLCLM QNHMVQAFAN SGNAIKPVSF IRDLKKIARH
210 220 230 240 250
FRFGNQEDAH EFLRYTIDAM QKACLNGYAK LDRQTQATTL VHQIFGGYLR
260 270 280 290 300
SRVKCSVCKS VSDTYDPYLD IALEIRQAAN IVRALELFVK SDVLSGENAY
310 320 330 340 350
MCAKCKKKVP ASKRFTIHRT SNVLTLSLKR FANFSGGKIT KDVGYPEFLN
360 370 380 390 400
IRPYMSQSSG DPVMYGLYAV LVHSGYSCHA GHYYCYVKAS NGQWYQMNDS
410 420 430 440 450
LVHSSNVKVV LNQQAYVLFY LRIPGSKKSP EGPVSRVGAT LPSRPKVVPE
460 470 480 490 500
HSKKSPGNGV VPSPLMAKRQ DSVMMRKLPA PEEVGVPVSR NGSLPGLKLQ
510 520 530 540 550
NGCAPAKTPA GSPSPRLTPT PTHMPTILDE PGKKVKKSAP LQSLTTSPTT
560 570 580 590 600
SQGSPGTGES RSQRPGSWAS RDTIFSTSPK LLARAITNGH RLKGEGSGVD
610 620 630 640 650
LEKGDSSSSS PEHSASSDPA KAPQTAESRA AHACDSQGTN CPTAGHPKAL
660 670 680 690 700
LNGVDAKMVK LKSPALSSTT TEPTSLMSPP PAKKLALSAK KASTLRRATG
710 720 730 740 750
NDIGSPSPSA FCDLTSPMKA THPVVASTGP VSKTRTAAPA PRPSTHPHSA
760 770 780 790 800
SLSSSSAKPL GTSEPQSCRP SAWTPLPQVN GHFTSHLHQL PEASEALHSP
810 820 830 840 850
SKKRKKTPNG DPQRLGIDTL LPQCLRGAPA AARRKRKKRC SEGEGATAPK
860 870 880 890 900
QEGQFQDQSW SSGSQKEEGT QPQVNGHQVS HILDSYHVSS RKRRKRKRSE
910 920 930 940 950
GLSQEATPSQ DLIQHSCSPV DHSEPEARTE LQKKKKKKRR KRKPEPQQDE
960 970 980 990 1000
ESKHPGDQRS PRPSVTPVPA LSVNGHLPSD CLGLGQAPLV TWNRDQEPDV
1010 1020 1030 1040 1050
VQALLQDSSD KAYGKKVLTW DGEPSAISQD AIKDSRLART QTVVDDWDEE
1060 1070 1080 1090
FDRGKEKKIK KFKREKKRNF NAFQKLQSRR NFWSVTHPAK VASLSYRR
Length:1,098
Mass (Da):119,913
Last modified:April 8, 2008 - v1
Checksum:i5853578D7BFD84DF
GO

Sequence cautioni

The sequence BAC98173.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti452 – 4521S → P in BAC98173 (PubMed:14621295).Curated
Sequence conflicti486 – 4861V → I in BAC98173 (PubMed:14621295).Curated
Sequence conflicti541 – 5411L → P in BAC98173 (PubMed:14621295).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL591404 Genomic DNA. Translation: CAM23104.1.
AK129363 mRNA. Translation: BAC98173.1. Different initiation.
CCDSiCCDS48998.1.
RefSeqiNP_001028700.1. NM_001033528.1.
XP_006534365.1. XM_006534302.2.
XP_006534366.1. XM_006534303.2.
XP_006534367.1. XM_006534304.2.
UniGeneiMm.232293.

Genome annotation databases

EnsembliENSMUST00000092382; ENSMUSP00000090036; ENSMUSG00000033909.
ENSMUST00000106296; ENSMUSP00000101903; ENSMUSG00000033909.
GeneIDi72344.
KEGGimmu:72344.
UCSCiuc007mot.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL591404 Genomic DNA. Translation: CAM23104.1.
AK129363 mRNA. Translation: BAC98173.1. Different initiation.
CCDSiCCDS48998.1.
RefSeqiNP_001028700.1. NM_001033528.1.
XP_006534365.1. XM_006534302.2.
XP_006534366.1. XM_006534303.2.
XP_006534367.1. XM_006534304.2.
UniGeneiMm.232293.

3D structure databases

ProteinModelPortaliB1AQJ2.
SMRiB1AQJ2. Positions 122-420.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

MEROPSiC19.042.

Proteomic databases

MaxQBiB1AQJ2.
PaxDbiB1AQJ2.
PRIDEiB1AQJ2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000092382; ENSMUSP00000090036; ENSMUSG00000033909.
ENSMUST00000106296; ENSMUSP00000101903; ENSMUSG00000033909.
GeneIDi72344.
KEGGimmu:72344.
UCSCiuc007mot.2. mouse.

Organism-specific databases

CTDi57602.
MGIiMGI:1919594. Usp36.
RougeiSearch...

Phylogenomic databases

eggNOGiCOG5533.
GeneTreeiENSGT00780000121889.
HOGENOMiHOG000154759.
InParanoidiB1AQJ2.
KOiK11855.
OMAiHQDPPWH.
OrthoDBiEOG72VH5K.
PhylomeDBiB1AQJ2.
TreeFamiTF315281.

Miscellaneous databases

ChiTaRSiUsp36. mouse.
NextBioi336077.
PROiB1AQJ2.
SOURCEiSearch...

Gene expression databases

BgeeiB1AQJ2.
ExpressionAtlasiB1AQJ2. baseline and differential.
GenevestigatoriB1AQJ2.

Family and domain databases

InterProiIPR001394. Peptidase_C19_UCH.
IPR018200. USP_CS.
IPR028889. USP_dom.
[Graphical view]
PfamiPF00443. UCH. 1 hit.
[Graphical view]
PROSITEiPS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  2. "Prediction of the coding sequences of mouse homologues of KIAA gene: III. The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
    Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S., Saga Y., Nagase T., Ohara O., Koga H.
    DNA Res. 10:167-180(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-937.
    Tissue: Embryonic tail.

Entry informationi

Entry nameiUBP36_MOUSE
AccessioniPrimary (citable) accession number: B1AQJ2
Secondary accession number(s): Q6ZPQ7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 7, 2009
Last sequence update: April 8, 2008
Last modified: April 1, 2015
This is version 54 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.