B1AQJ2 (UBP36_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ubiquitin carboxyl-terminal hydrolase 36
EC=3.4.19.12

Alternative name(s):
Deubiquitinating enzyme 36
Ubiquitin thioesterase 36
Ubiquitin-specific-processing protease 36

Gene names

Name:	Usp36
Synonyms:	Kiaa1453

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	1098 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	May be required for maintaining multiple types of adult stem cells. May function as a transcriptional repressor by continually deubiquiting histone H2B at the promoters of genes critical for cellular differentiation, thereby preventing histone H3 'Lys-4' trimethylation (H3K4) By similarity.
Catalytic activity	Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
Subunit structure	Interacts with PAF1 By similarity.
Subcellular location	Nucleus › nucleolus By similarity.
Sequence similarities	Belongs to the peptidase C19 family.
Sequence caution	The sequence BAC98173.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Cellular component	Nucleus
Molecular function	Hydrolase Protease Thiol protease
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	ubiquitin-dependent protein catabolic process Inferred from electronic annotation. Source: InterPro
Cellular_component	nucleolus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	cysteine-type peptidase activity Inferred from electronic annotation. Source: UniProtKB-KW ubiquitin thiolesterase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1098	1098	Ubiquitin carboxyl-terminal hydrolase 36		PRO_0000378494
Sites
	Active site	131	1	Nucleophile By similarity
	Active site	382	1	Proton acceptor By similarity
Amino acid modifications
	Modified residue	463	1	Phosphoserine By similarity
	Modified residue	578	1	Phosphoserine By similarity
	Modified residue	663	1	Phosphoserine By similarity
	Modified residue	678	1	Phosphoserine By similarity
	Modified residue	709	1	Phosphoserine By similarity
Experimental info
	Sequence conflict	452	1	S → P in BAC98173. Ref.2
	Sequence conflict	486	1	V → I in BAC98173. Ref.2
	Sequence conflict	541	1	L → P in BAC98173. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	B1AQJ2 [UniParc]. Last modified April 8, 2008. Version 1. Checksum: 5853578D7BFD84DF	FASTA	1,098	119,913
	10 20 30 40 50 60 MPIVDKLKEA LKPGRKDSAE DGDLGRLLAA SAKKVLLQRI EFEPASKSFS YQLESLKSKY 70 80 90 100 110 120 VLLSARAEGA SRHRSGDELQ ARKPGTERVS GSGGDGVPAP QKVLFPVERL SLRWERVFRV 130 140 150 160 170 180 GAGLHNLGNT CFLNSTIQCL TYTPPLANYL LSKEHARSCH QGGFCMLCLM QNHMVQAFAN 190 200 210 220 230 240 SGNAIKPVSF IRDLKKIARH FRFGNQEDAH EFLRYTIDAM QKACLNGYAK LDRQTQATTL 250 260 270 280 290 300 VHQIFGGYLR SRVKCSVCKS VSDTYDPYLD IALEIRQAAN IVRALELFVK SDVLSGENAY 310 320 330 340 350 360 MCAKCKKKVP ASKRFTIHRT SNVLTLSLKR FANFSGGKIT KDVGYPEFLN IRPYMSQSSG 370 380 390 400 410 420 DPVMYGLYAV LVHSGYSCHA GHYYCYVKAS NGQWYQMNDS LVHSSNVKVV LNQQAYVLFY 430 440 450 460 470 480 LRIPGSKKSP EGPVSRVGAT LPSRPKVVPE HSKKSPGNGV VPSPLMAKRQ DSVMMRKLPA 490 500 510 520 530 540 PEEVGVPVSR NGSLPGLKLQ NGCAPAKTPA GSPSPRLTPT PTHMPTILDE PGKKVKKSAP 550 560 570 580 590 600 LQSLTTSPTT SQGSPGTGES RSQRPGSWAS RDTIFSTSPK LLARAITNGH RLKGEGSGVD 610 620 630 640 650 660 LEKGDSSSSS PEHSASSDPA KAPQTAESRA AHACDSQGTN CPTAGHPKAL LNGVDAKMVK 670 680 690 700 710 720 LKSPALSSTT TEPTSLMSPP PAKKLALSAK KASTLRRATG NDIGSPSPSA FCDLTSPMKA 730 740 750 760 770 780 THPVVASTGP VSKTRTAAPA PRPSTHPHSA SLSSSSAKPL GTSEPQSCRP SAWTPLPQVN 790 800 810 820 830 840 GHFTSHLHQL PEASEALHSP SKKRKKTPNG DPQRLGIDTL LPQCLRGAPA AARRKRKKRC 850 860 870 880 890 900 SEGEGATAPK QEGQFQDQSW SSGSQKEEGT QPQVNGHQVS HILDSYHVSS RKRRKRKRSE 910 920 930 940 950 960 GLSQEATPSQ DLIQHSCSPV DHSEPEARTE LQKKKKKKRR KRKPEPQQDE ESKHPGDQRS 970 980 990 1000 1010 1020 PRPSVTPVPA LSVNGHLPSD CLGLGQAPLV TWNRDQEPDV VQALLQDSSD KAYGKKVLTW 1030 1040 1050 1060 1070 1080 DGEPSAISQD AIKDSRLART QTVVDDWDEE FDRGKEKKIK KFKREKKRNF NAFQKLQSRR 1090 NFWSVTHPAK VASLSYRR

References

[1]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6J.
[2]	"Prediction of the coding sequences of mouse homologues of KIAA gene: III. The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries." Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S., Saga Y., Nagase T., Ohara O., Koga H. DNA Res. 10:167-180(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-937. Tissue: Embryonic tail.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL591404 Genomic DNA. Translation: CAM23104.1. AK129363 mRNA. Translation: BAC98173.1. Different initiation.
IPI	IPI00929784.
RefSeq	NP_001028700.1. NM_001033528.1.
UniGene	Mm.232293.
3D structure databases
ProteinModelPortal	B1AQJ2.
SMR	B1AQJ2. Positions 122-420.
ModBase	Search...
Proteomic databases
PaxDb	B1AQJ2.
PRIDE	B1AQJ2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000092382; ENSMUSP00000090036; ENSMUSG00000033909. ENSMUST00000106296; ENSMUSP00000101903; ENSMUSG00000033909.
GeneID	72344.
KEGG	mmu:72344.
UCSC	uc007mot.2. mouse.
Organism-specific databases
CTD	57602.
MGI	MGI:1919594. Usp36.
Rouge	Search...
Phylogenomic databases
eggNOG	COG5533.
GeneTree	ENSGT00670000097591.
HOGENOM	HOG000154759.
InParanoid	B1AQJ2.
KO	K11855.
OMA	VTHPAKA.
OrthoDB	EOG4V9TQ1.
Gene expression databases
ArrayExpress	B1AQJ2.
Bgee	B1AQJ2.
Genevestigator	B1AQJ2.
Family and domain databases
InterPro	IPR018200. Pept_C19ubi-hydrolase_C_CS. IPR001394. Peptidase_C19. [Graphical view]
Pfam	PF00443. UCH. 1 hit. [Graphical view]
PROSITE	PS00972. UCH_2_1. 1 hit. PS00973. UCH_2_2. 1 hit. PS50235. UCH_2_3. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	USP36. mouse.
NextBio	336077.
SOURCE	Search...

Entry information

Entry name

UBP36_MOUSE

Accession

Primary (citable) accession number: B1AQJ2
Secondary accession number(s): Q6ZPQ7

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 7, 2009
Last sequence update:	April 8, 2008
Last modified:	May 1, 2013
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program