B1A4F7 (VDDP4_VESVU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Venom dipeptidyl peptidase 4 EC=3.4.14.5 Alternative name(s): Venom dipeptidyl peptidase IV Allergen=Ves v 3
Organism	Vespula vulgaris (Yellow jacket) (Wasp)
Taxonomic identifier	7454 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Panarthropoda › Arthropoda › Mandibulata › Pancrustacea › Hexapoda › Insecta › Dicondylia › Pterygota › Neoptera › Endopterygota › Hymenoptera › Apocrita › Aculeata › Vespoidea › Vespidae › Vespinae › Vespula

Protein attributes

Sequence length	776 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Venom dipeptidyl-peptidase which removes N-terminal dipeptides sequentially from polypeptides having unsubstituted N-termini provided that the penultimate residue is proline. May process venom proteins into their activ forms and/or modulate the chemotactic activity of immune cells after the insect sting. Ref.1
Catalytic activity	Release of an N-terminal dipeptide, Xaa-Yaa-|-Zaa-, from a polypeptide, preferentially when Yaa is Pro, provided Zaa is neither Pro nor hydroxyproline. Ref.1
Enzyme regulation	Inhibited by diprotin A. Ref.1
Subcellular location	Secreted Ref.1.
Tissue specificity	Expressed by the venom gland. Ref.1
Allergenic properties	Causes an allergic reaction in human. Ref.1
Sequence similarities	Belongs to the peptidase S9B family. DPPIV subfamily.

Ontologies

Keywords
Cellular component	Secreted
Disease	Allergen
Domain	Signal
Molecular function	Aminopeptidase Hydrolase Protease
PTM	Disulfide bond Glycoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological_process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell membrane Inferred from electronic annotation. Source: InterPro
Molecular_function	aminopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW serine-type peptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 25	25	Potential
	Chain	26 – 776	751	Venom dipeptidyl peptidase 4		PRO_0000401924
Sites
	Active site	638	1	Charge relay system By similarity
	Active site	717	1	Charge relay system By similarity
	Active site	749	1	Charge relay system By similarity
Amino acid modifications
	Glycosylation	44	1	N-linked (GlcNAc...) Potential
	Glycosylation	66	1	N-linked (GlcNAc...) Potential
	Glycosylation	329	1	N-linked (GlcNAc...) Potential
	Glycosylation	504	1	N-linked (GlcNAc...) Potential
	Glycosylation	577	1	N-linked (GlcNAc...) Potential
	Glycosylation	688	1	N-linked (GlcNAc...) Potential
	Glycosylation	693	1	N-linked (GlcNAc...) Potential
	Disulfide bond	449 ↔ 452		By similarity
	Disulfide bond	462 ↔ 480		By similarity
	Disulfide bond	658 ↔ 769		By similarity

Sequences

	Sequence		Length	Mass (Da)
	B1A4F7 [UniParc]. Last modified April 8, 2008. Version 1. Checksum: 04A9ECBC4FFFC0B2	FASTA	776	88,924
	10 20 30 40 50 60 MVPLRSFVLL NSLFLVLLAA RTVVTRVIDK DNSDRIVKTQ NDQNLSKVPF NLEETYTADF 70 80 90 100 110 120 LAYVFNGTWT SDTTIVYTDR RTGDILQFDV IKQRSTLIVD SSVMDAYIVS NYVLSPKGRY 130 140 150 160 170 180 LLIGYDLKKG YRYSTFMRYV IYDIEHRAYH KIGNDMHIAL AKWAPLTDDL IYILDNDIYY 190 200 210 220 230 240 MRFSNNGFND VQRVTYDGIS GIVYNGVPDW VYEEEVLQDS SAIWFSPDGN HLAYASFDDR 250 260 270 280 290 300 NVQEILYLHY GEPGNLDDQY PTEVKIKYPK VGTLNPVVSL TLVDLHDPTL NKIDLKAPHY 310 320 330 340 350 360 AVGTDNLLYN VQWKDFDHVV VTWSNRVQNK TEIVWYNMYG EIVKTLHVVE HKGWLDIKHL 370 380 390 400 410 420 FFYKGSVYIR KLQPSGTKAG RFHHVTRYDE TFKQSPTQMD LTPDAIEVQN ICTIDQSNGR 430 440 450 460 470 480 IYYLASGLGK PSQKNLYSVP ADGSEKPTCI SCNVLTPEGN VCTYADAIFS PLGQYYVLVC 490 500 510 520 530 540 HGPDPAFVSI FNNAHQKVYS WENNLSLRKK LAKRHLPLVK DLDVRANGYE SKVRLFLPHN 550 560 570 580 590 600 FDESKSYPML VNVYAGPNTL KIIDAASYGH QVYMTTNRSV IYAYIDGRGS SNKGSKMLFS 610 620 630 640 650 660 IYRKLGTVEV EDQITVTRQL QEMFPWIDSK RTGVWGWSYG GFSTAMILAK DTSFVFKCGI 670 680 690 700 710 720 AIAPVSSWIY YDSIYTERFM GFPTPEDNLS GYNETDVSRR VEDIRGKKFM LIHGSGDDNV 730 740 750 760 770 HYQQSLALAK ALEKADVMFE QITYTDEAHA LFGVLPHLYH TMDRFWSDCF SLSHAH

References

[1]

"Identification, recombinant expression, and characterization of the 100 kDa high molecular weight hymenoptera venom allergens Api m 5 and Ves v 3." Blank S., Seismann H., Bockisch B., Braren I., Cifuentes L., McIntyre M., Ruhl D., Ring J., Bredehorst R., Ollert M.W., Grunwald T., Spillner E. J. Immunol. 184:5403-5413(2010) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 83-86; 301-309; 536-539; 548-555 AND 606-613, MASS SPECTROMETRY, FUNCTION, CATALYTIC ACTIVITY, ENZYME REGULATION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, ALLERGEN. Tissue: Venom and Venom duct.

Cross-references

Sequence databases
EMBL GenBank DDBJ	EU420987 mRNA. Translation: ACA00159.1.
3D structure databases
ProteinModelPortal	B1A4F7.
ModBase	Search...
Protein family/group databases
Allergome	5767. Ves v 3. 5768. Ves v 3.0101.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR001375. Peptidase_S9. IPR002469. Peptidase_S9B. [Graphical view]
Pfam	PF00930. DPPIV_N. 1 hit. PF00326. Peptidase_S9. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

VDDP4_VESVU

Accession

Primary (citable) accession number: B1A4F7

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 30, 2010
Last sequence update:	April 8, 2008
Last modified:	October 3, 2012
This is version 17 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)