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Protein

Venom dipeptidyl peptidase 4

Gene
N/A
Organism
Vespula vulgaris (Yellow jacket) (Wasp)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Venom dipeptidyl-peptidase which removes N-terminal dipeptides sequentially from polypeptides having unsubstituted N-termini provided that the penultimate residue is proline. May process venom proteins into their active forms and/or modulate the chemotactic activity of immune cells after the insect sting.1 Publication

Catalytic activityi

Release of an N-terminal dipeptide, Xaa-Yaa-|-Zaa-, from a polypeptide, preferentially when Yaa is Pro, provided Zaa is neither Pro nor hydroxyproline.1 Publication

Enzyme regulationi

Inhibited by diprotin A.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei638 – 6381Charge relay systemBy similarity
Active sitei717 – 7171Charge relay systemBy similarity
Active sitei749 – 7491Charge relay systemBy similarity

GO - Molecular functioni

  1. aminopeptidase activity Source: UniProtKB-KW
  2. serine-type peptidase activity Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Aminopeptidase, Hydrolase, Protease

Protein family/group databases

MEROPSiS09.A64.

Names & Taxonomyi

Protein namesi
Recommended name:
Venom dipeptidyl peptidase 4 (EC:3.4.14.5)
Alternative name(s):
Venom dipeptidyl peptidase IV
Allergen: Ves v 3
OrganismiVespula vulgaris (Yellow jacket) (Wasp)
Taxonomic identifieri7454 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaHymenopteraApocritaAculeataVespoideaVespidaeVespinaeVespula

Subcellular locationi

Secreted 1 Publication

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-SubCell
  2. membrane Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Allergenic propertiesi

Causes an allergic reaction in human.1 Publication

Keywords - Diseasei

Allergen

Protein family/group databases

Allergomei5767. Ves v 3.
5768. Ves v 3.0101.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2525Sequence AnalysisAdd
BLAST
Chaini26 – 776751Venom dipeptidyl peptidase 4PRO_0000401924Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi44 – 441N-linked (GlcNAc...)Sequence Analysis
Glycosylationi66 – 661N-linked (GlcNAc...)Sequence Analysis
Glycosylationi329 – 3291N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi449 ↔ 452By similarity
Disulfide bondi462 ↔ 480By similarity
Glycosylationi504 – 5041N-linked (GlcNAc...)Sequence Analysis
Glycosylationi577 – 5771N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi658 ↔ 769By similarity
Glycosylationi688 – 6881N-linked (GlcNAc...)Sequence Analysis
Glycosylationi693 – 6931N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Expressioni

Tissue specificityi

Expressed by the venom gland.1 Publication

Structurei

3D structure databases

ProteinModelPortaliB1A4F7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase S9B family. DPPIV subfamily.Curated

Keywords - Domaini

Signal

Family and domain databases

Gene3Di2.140.10.30. 1 hit.
3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR001375. Peptidase_S9.
IPR002469. Peptidase_S9B.
[Graphical view]
PfamiPF00930. DPPIV_N. 1 hit.
PF00326. Peptidase_S9. 1 hit.
[Graphical view]
SUPFAMiSSF53474. SSF53474. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

B1A4F7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVPLRSFVLL NSLFLVLLAA RTVVTRVIDK DNSDRIVKTQ NDQNLSKVPF
60 70 80 90 100
NLEETYTADF LAYVFNGTWT SDTTIVYTDR RTGDILQFDV IKQRSTLIVD
110 120 130 140 150
SSVMDAYIVS NYVLSPKGRY LLIGYDLKKG YRYSTFMRYV IYDIEHRAYH
160 170 180 190 200
KIGNDMHIAL AKWAPLTDDL IYILDNDIYY MRFSNNGFND VQRVTYDGIS
210 220 230 240 250
GIVYNGVPDW VYEEEVLQDS SAIWFSPDGN HLAYASFDDR NVQEILYLHY
260 270 280 290 300
GEPGNLDDQY PTEVKIKYPK VGTLNPVVSL TLVDLHDPTL NKIDLKAPHY
310 320 330 340 350
AVGTDNLLYN VQWKDFDHVV VTWSNRVQNK TEIVWYNMYG EIVKTLHVVE
360 370 380 390 400
HKGWLDIKHL FFYKGSVYIR KLQPSGTKAG RFHHVTRYDE TFKQSPTQMD
410 420 430 440 450
LTPDAIEVQN ICTIDQSNGR IYYLASGLGK PSQKNLYSVP ADGSEKPTCI
460 470 480 490 500
SCNVLTPEGN VCTYADAIFS PLGQYYVLVC HGPDPAFVSI FNNAHQKVYS
510 520 530 540 550
WENNLSLRKK LAKRHLPLVK DLDVRANGYE SKVRLFLPHN FDESKSYPML
560 570 580 590 600
VNVYAGPNTL KIIDAASYGH QVYMTTNRSV IYAYIDGRGS SNKGSKMLFS
610 620 630 640 650
IYRKLGTVEV EDQITVTRQL QEMFPWIDSK RTGVWGWSYG GFSTAMILAK
660 670 680 690 700
DTSFVFKCGI AIAPVSSWIY YDSIYTERFM GFPTPEDNLS GYNETDVSRR
710 720 730 740 750
VEDIRGKKFM LIHGSGDDNV HYQQSLALAK ALEKADVMFE QITYTDEAHA
760 770
LFGVLPHLYH TMDRFWSDCF SLSHAH
Length:776
Mass (Da):88,924
Last modified:April 8, 2008 - v1
Checksum:i04A9ECBC4FFFC0B2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EU420987 mRNA. Translation: ACA00159.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EU420987 mRNA. Translation: ACA00159.1.

3D structure databases

ProteinModelPortaliB1A4F7.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

Allergomei5767. Ves v 3.
5768. Ves v 3.0101.
MEROPSiS09.A64.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di2.140.10.30. 1 hit.
3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR001375. Peptidase_S9.
IPR002469. Peptidase_S9B.
[Graphical view]
PfamiPF00930. DPPIV_N. 1 hit.
PF00326. Peptidase_S9. 1 hit.
[Graphical view]
SUPFAMiSSF53474. SSF53474. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Identification, recombinant expression, and characterization of the 100 kDa high molecular weight hymenoptera venom allergens Api m 5 and Ves v 3."
    Blank S., Seismann H., Bockisch B., Braren I., Cifuentes L., McIntyre M., Ruhl D., Ring J., Bredehorst R., Ollert M.W., Grunwald T., Spillner E.
    J. Immunol. 184:5403-5413(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 83-86; 301-309; 536-539; 548-555 AND 606-613, IDENTIFICATION BY MASS SPECTROMETRY, FUNCTION, CATALYTIC ACTIVITY, ENZYME REGULATION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, ALLERGEN.
    Tissue: Venom and Venom duct.

Entry informationi

Entry nameiVDDP4_VESVU
AccessioniPrimary (citable) accession number: B1A4F7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 30, 2010
Last sequence update: April 8, 2008
Last modified: January 7, 2015
This is version 24 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Allergens
    Nomenclature of allergens and list of entries
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.