B0YBU9 (MANBB_ASPFC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable beta-mannosidase B
EC=3.2.1.25

Alternative name(s):
Mannanase B
Short name=Mannase B

Gene names

Name:	mndB
ORF Names:	AFUB_087900

Organism

Neosartorya fumigata (strain CEA10 / CBS 144.89 / FGSC A1163) (Aspergillus fumigatus) [Complete proteome]

Taxonomic identifier

451804 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Pezizomycotina › leotiomyceta › Eurotiomycetes › Eurotiomycetidae › Eurotiales › Trichocomaceae › mitosporic Trichocomaceae › Aspergillus › Neosartorya fumigata

Protein attributes

Sequence length	845 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Exoglycosidase that cleaves the single beta-linked mannose residue from the non-reducing end of all N-linked glycoprotein oligosaccharides. Involved in the degradation of mannan and galactomannan By similarity.
Catalytic activity	Hydrolysis of terminal, non-reducing beta-D-mannose residues in beta-D-mannosides.
Pathway	Glycan metabolism; N-glycan degradation.
Subcellular location	Secreted Probable.
Sequence similarities	Belongs to the glycosyl hydrolase 2 family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Polysaccharide degradation
Cellular component	Secreted
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	polysaccharide catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	beta-mannosidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – ?		Potential
	Chain	? – 845		Probable beta-mannosidase B		PRO_0000394652
Sites
	Active site	432	1	Proton donor By similarity
Amino acid modifications
	Glycosylation	252	1	N-linked (GlcNAc...) Potential
	Glycosylation	717	1	N-linked (GlcNAc...) Potential
	Glycosylation	723	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	B0YBU9 [UniParc]. Last modified April 8, 2008. Version 1. Checksum: B7D783CF578985E3	FASTA	845	97,047
	10 20 30 40 50 60 MSKLQQFPLS KGWSFRDNEA TSEDAWMPVP VVPSVVHQDL QANNKLKDPY IGFNELEARW 70 80 90 100 110 120 VNEKSWTYKT VFQKPAAPAG SCIVLAFDGL DTFAKVKLDG NVILENDNMF LARRVDVTKA 130 140 150 160 170 180 LEAEGDHVLE IDFDCAFLRA KELRKQDPRH NWASFNGDPS RLSVRKAQYH WGWDWGPVLM 190 200 210 220 230 240 TAGIWREVRL EVYSARVADL WTEVQLASDH QSAQVTAFVE VESVHSGSHR ACFTLSLHGQ 250 260 270 280 290 300 EITREEIGVT ENGTAKATFD VKEPSLWWPH GYGDATLYEV SVSLVKEQEE LHRVSKKFGI 310 320 330 340 350 360 RTAEVIQRPD KHGKSFFFRV NGVDIFCGGS CWIPADNLLP SITAERYRKW IELMVHGRQV 370 380 390 400 410 420 MIRVWGGGIY EDYSFYDACD ELGVLVWQDF MFGCGNYPTW PNLLESIRKE SVYNVRRLRH 430 440 450 460 470 480 HPSIVIWVGN NEDYQVQEQA GLTYNYEDKD PENWLKTDFP ARYIYEKLLP EVVQEYSPGT 490 500 510 520 530 540 FYHPGSPWGD GKTTSDPTVG DMHQWNVWHG TQEKYQIFDT LGGRFNSEFG MEAFPHMSTI 550 560 570 580 590 600 DYFVENEADK YPQSHVLDFH NKADGHERRI ATYLVENLRT ATDLETHIYL TQVVQAETMM 610 620 630 640 650 660 FGYRGWRRQW GDERHCGGAL LWQLNDCWPT ISWAIVDYFL RPKPAFYAVA RVLNPIAVGV 670 680 690 700 710 720 RREHHDWSVT HAQPPKTSKF ELWVASSRQQ EIQGTVELRF LSVNTGLEVR ERIVHENVSI 730 740 750 760 770 780 VPNGTTNLIV DGLIDHTVHS EPHVLAARIW VDGQLVARDV DWPQPFKYLD LSDRGLEVKK 790 800 810 820 830 840 ISESEDEQTL LISTKKPVKC LVFEEREGVR ISDSAMDIVP GDDQRVTIKG LKPGDAPLKY KFLGQ

References

[1]

"Genomic islands in the pathogenic filamentous fungus Aspergillus fumigatus." Fedorova N.D., Khaldi N., Joardar V.S., Maiti R., Amedeo P., Anderson M.J., Crabtree J., Silva J.C., Badger J.H., Albarraq A., Angiuoli S., Bussey H., Bowyer P., Cotty P.J., Dyer P.S., Egan A., Galens K., Fraser-Liggett C.M. , Haas B.J., Inman J.M., Kent R., Lemieux S., Malavazi I., Orvis J., Roemer T., Ronning C.M., Sundaram J.P., Sutton G., Turner G., Venter J.C., White O.R., Whitty B.R., Youngman P., Wolfe K.H., Goldman G.H., Wortman J.R., Jiang B., Denning D.W., Nierman W.C. PLoS Genet. 4:E1000046-E1000046(2008) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CEA10 / CBS 144.89 / FGSC A1163.

Cross-references

Sequence databases
EMBL GenBank DDBJ	DS499601 Genomic DNA. Translation: EDP48080.1.
3D structure databases
ProteinModelPortal	B0YBU9.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	CADAFUBT00008704; CADAFUBP00008544; CADAFUBG00008704.
Phylogenomic databases
HOGENOM	HOG000186861.
Enzyme and pathway databases
UniPathway	UPA00280.
Family and domain databases
Gene3D	2.60.40.320. 1 hit. 3.20.20.80. 2 hits.
InterPro	IPR008979. Galactose-bd-like. IPR013812. Glyco_hydro_2/20_Ig-like. IPR006102. Glyco_hydro_2_Ig-like. IPR006104. Glyco_hydro_2_N. IPR013781. Glyco_hydro_catalytic_dom. IPR017853. Glycoside_hydrolase_SF. [Graphical view]
Pfam	PF00703. Glyco_hydro_2. 1 hit. PF02837. Glyco_hydro_2_N. 1 hit. [Graphical view]
SUPFAM	SSF49785. Gal_bind_like. 1 hit. SSF49303. Glyco_hydro_2Ig. 1 hit. SSF51445. Glyco_hydro_cat. 1 hit.
PROSITE	PS00719. GLYCOSYL_HYDROL_F2_1. False negative. PS00608. GLYCOSYL_HYDROL_F2_2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MANBB_ASPFC

Accession

Primary (citable) accession number: B0YBU9

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 15, 2010
Last sequence update:	April 8, 2008
Last modified:	May 1, 2013
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program