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B0Y9E7 (MANF_ASPFC) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 32. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable mannan endo-1,4-beta-mannosidase F

EC=3.2.1.78
Alternative name(s):
Endo-beta-1,4-mannanase F
Gene names
Name:manF
ORF Names:AFUB_080770
OrganismNeosartorya fumigata (strain CEA10 / CBS 144.89 / FGSC A1163) (Aspergillus fumigatus) [Complete proteome]
Taxonomic identifier451804 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus

Protein attributes

Sequence length438 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Endo-1,4-mannanase, a crucial enzyme for depolymerization of seed galactomannans and wood galactoglucomannans By similarity.

Catalytic activity

Random hydrolysis of (1->4)-beta-D-mannosidic linkages in mannans, galactomannans and glucomannans.

Subcellular location

Secreted By similarity.

Domain

Has a modular structure: a carbohydrate-binding module (CBM) at the N-terminus, a linker rich in serines, and a C-terminal endo-1,4-mannanase catalytic module. The genes for catalytic modules and CBMs seem to have evolved separately and have been linked by gene fusion.

Sequence similarities

Belongs to the glycosyl hydrolase 5 (cellulase A) family.

Contains 1 CBM1 (fungal-type carbohydrate-binding) domain.

Sequence caution

The sequence EDP48640.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Ontologies

Keywords
   Biological processCarbohydrate metabolism
   Cellular componentSecreted
   DomainSignal
   Molecular functionGlycosidase
Hydrolase
   PTMGlycoprotein
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processcarbohydrate metabolic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentextracellular region

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functioncellulose binding

Inferred from electronic annotation. Source: InterPro

mannan endo-1,4-beta-mannosidase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1818 Potential
Chain19 – 438420Probable mannan endo-1,4-beta-mannosidase F
PRO_0000393713

Regions

Domain19 – 5436CBM1
Region60 – 9637Ser-rich linker
Region97 – 438342Catalytic

Sites

Active site2641Proton donor By similarity
Active site3731Nucleophile By similarity

Amino acid modifications

Glycosylation2771N-linked (GlcNAc...) Potential

Sequences

Sequence LengthMass (Da)Tools
B0Y9E7 [UniParc].

Last modified April 20, 2010. Version 2.
Checksum: 8E55123506BD7E24

FASTA43847,327
        10         20         30         40         50         60 
MHPLPSVALL SAIGAVAAQV GPWGQCGGRS YTGETSCVSG WSCVLFNEWY SQCQPATTTS 

        70         80         90        100        110        120 
TSSVSATAAP SSTSSSKESV PSATTSKKPV PTGSSSFVKA DGLKFNIDGE TKYFAGTNAY 

       130        140        150        160        170        180 
WLPFLTNDAD VDSVMDNLQK AGLKILRTWG FNDVNSKPSS GTVYFQLHDP STGTTTINTG 

       190        200        210        220        230        240 
ADGLQRLDYV VSAAEKRGIK LLIPLVNNWD DYGGMNAYVK AYGGSKTEWY TNSKIQSVYQ 

       250        260        270        280        290        300 
AYIKAVVSRY RDSPAIMAWE LSNEARCQGC STDVIYNWTA KTSAYIKSLD PNHMVATGDE 

       310        320        330        340        350        360 
GMGVTVDSDG SYPYSTYEGS DFAKNLAAPD IDFGVFHLYT EDWGIKDNSW GNGWVTSHAK 

       370        380        390        400        410        420 
VCKAAGKPCL FEEYGLKDDH CSASLTWQKT SVSSGMAADL FWQYGQTLST GPSPNDHFTI 

       430 
YYGTSDWQCG VADHLSTL 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
DS499600 Genomic DNA. Translation: EDP48640.1. Different initiation.

3D structure databases

ProteinModelPortalB0Y9E7.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Phylogenomic databases

HOGENOMHOG000169951.
OrthoDBEOG7M3J90.
PhylomeDBB0Y9E7.

Family and domain databases

Gene3D3.20.20.80. 1 hit.
InterProIPR000254. Cellulose-bd_dom_fun.
IPR001547. Glyco_hydro_5.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamPF00734. CBM_1. 1 hit.
PF00150. Cellulase. 1 hit.
[Graphical view]
SMARTSM00236. fCBD. 1 hit.
[Graphical view]
SUPFAMSSF51445. SSF51445. 1 hit.
SSF57180. SSF57180. 1 hit.
PROSITEPS00562. CBM1_1. 1 hit.
PS51164. CBM1_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMANF_ASPFC
AccessionPrimary (citable) accession number: B0Y9E7
Entry history
Integrated into UniProtKB/Swiss-Prot: April 20, 2010
Last sequence update: April 20, 2010
Last modified: April 16, 2014
This is version 32 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries