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Protein

Probable dipeptidyl peptidase 4

Gene

dpp4

Organism
Neosartorya fumigata (strain CEA10 / CBS 144.89 / FGSC A1163) (Aspergillus fumigatus)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Extracellular dipeptidyl-peptidase which removes N-terminal dipeptides sequentially from polypeptides having unsubstituted N-termini provided that the penultimate residue is proline. Contributes to pathogenicity.1 Publication

Catalytic activityi

Release of an N-terminal dipeptide, Xaa-Yaa-|-Zaa-, from a polypeptide, preferentially when Yaa is Pro, provided Zaa is neither Pro nor hydroxyproline.

Kineticsi

  1. KM=0.38 mM for Gly-Pro pNA and Ala-Pro pNA1 Publication
  2. KM=0.15 mM for Arg-Pro pNA1 Publication

    pH dependencei

    Optimum pH is 6.7 to 7.0.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei613 – 6131Charge relay systemBy similarity
    Active sitei690 – 6901Charge relay systemBy similarity
    Active sitei725 – 7251Charge relay systemBy similarity

    GO - Molecular functioni

    GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Aminopeptidase, Hydrolase, Protease, Serine protease

    Keywords - Biological processi

    Virulence

    Protein family/group databases

    ESTHERiaspfu-DPP4. DPP4N_Peptidase_S9.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Probable dipeptidyl peptidase 4 (EC:3.4.14.5)
    Alternative name(s):
    Dipeptidyl peptidase IV
    Short name:
    DPP IV
    Short name:
    DppIV
    Gene namesi
    Name:dpp4
    ORF Names:AFUB_066450
    OrganismiNeosartorya fumigata (strain CEA10 / CBS 144.89 / FGSC A1163) (Aspergillus fumigatus)
    Taxonomic identifieri451804 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
    ProteomesiUP000001699 Componenti: Unassembled WGS sequence

    Subcellular locationi

    GO - Cellular componenti

    Complete GO annotation...

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1414Sequence AnalysisAdd
    BLAST
    Chaini15 – 765751Probable dipeptidyl peptidase 4PRO_0000397810Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi35 – 351N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi78 – 781N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi101 – 1011N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi110 – 1101N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi169 – 1691N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi218 – 2181N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi465 – 4651N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi490 – 4901N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi665 – 6651N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Structurei

    3D structure databases

    ProteinModelPortaliB0Y6C5.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the peptidase S9B family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    HOGENOMiHOG000189891.
    OrthoDBiEOG72VHFG.
    PhylomeDBiB0Y6C5.

    Family and domain databases

    Gene3Di2.140.10.30. 1 hit.
    3.40.50.1820. 1 hit.
    InterProiIPR029058. AB_hydrolase.
    IPR001375. Peptidase_S9.
    IPR002469. Peptidase_S9B.
    [Graphical view]
    PfamiPF00930. DPPIV_N. 1 hit.
    PF00326. Peptidase_S9. 1 hit.
    [Graphical view]
    SUPFAMiSSF53474. SSF53474. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    B0Y6C5-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MKWSILLLVG CAAAIDVPRQ PYAPTGSGKK RLTFNETVVK RAISPSAISV
    60 70 80 90 100
    EWISTSEDGD YVYQDQDGSL KIQSIVTNHT QTLVPADKVP EDAYSYWIHP
    110 120 130 140 150
    NLSSVLWATN YTKQYRYSYF ADYFIQDVQS MKLRPLAPDQ SGDIQYAQWS
    160 170 180 190 200
    PTGDAIAFVR DNNVFVWTNA STSQITNDGG PDLFNGVPDW IYEEEILGDR
    210 220 230 240 250
    FALWFSPDGA YLAFLRFNET GVPTFTVPYY MDNEEIAPPY PRELELRYPK
    260 270 280 290 300
    VSQTNPTVEL NLLELRTGER TPVPIDAFDA KELIIGEVAW LTGKHDVVAV
    310 320 330 340 350
    KAFNRVQDRQ KVVAVDVASL RSKTISERDG TDGWLDNLLS MAYIGPIGES
    360 370 380 390 400
    KEEYYIDISD QSGWAHLWLF PVAGGEPIAL TKGEWEVTNI LSIDKPRQLV
    410 420 430 440 450
    YFLSTKHHST ERHLYSVSWK TKEITPLVDD TVPAVWSASF SSQGGYYILS
    460 470 480 490 500
    YRGPDVPYQD LYAINSTAPL RTITSNAAVL NALKEYTLPN ITYFELALPS
    510 520 530 540 550
    GETLNVMQRL PVKFSPKKKY PVLFTPYGGP GAQEVSKAWQ ALDFKAYIAS
    560 570 580 590 600
    DPELEYITWT VDNRGTGYKG RAFRCQVASR LGELEAADQV FAAQQAAKLP
    610 620 630 640 650
    YVDAQHIAIW GWSYGGYLTG KVIETDSGAF SLGVQTAPVS DWRFYDSMYT
    660 670 680 690 700
    ERYMKTLESN AAGYNASAIR KVAGYKNVRG GVLIQHGTGD DNVHFQNAAA
    710 720 730 740 750
    LVDTLVGAGV TPEKLQVQWF TDSDHGIRYH GGNVFLYRQL SKRLYEEKKR
    760
    KEKGEAHQWS KKSVL
    Length:765
    Mass (Da):85,876
    Last modified:April 8, 2008 - v1
    Checksum:i544F27B6134F9B1B
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti117 – 1171Y → H in AAC34310 (PubMed:9234752).Curated
    Sequence conflicti161 – 1611D → G in AAC34310 (PubMed:9234752).Curated
    Sequence conflicti538 – 5381A → P in AAC34310 (PubMed:9234752).Curated

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    U87950 Genomic DNA. Translation: AAC34310.1.
    DS499598 Genomic DNA. Translation: EDP50310.1.

    Genome annotation databases

    EnsemblFungiiCADAFUBT00006595; CADAFUBP00006467; CADAFUBG00006595.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    U87950 Genomic DNA. Translation: AAC34310.1.
    DS499598 Genomic DNA. Translation: EDP50310.1.

    3D structure databases

    ProteinModelPortaliB0Y6C5.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein family/group databases

    ESTHERiaspfu-DPP4. DPP4N_Peptidase_S9.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblFungiiCADAFUBT00006595; CADAFUBP00006467; CADAFUBG00006595.

    Phylogenomic databases

    HOGENOMiHOG000189891.
    OrthoDBiEOG72VHFG.
    PhylomeDBiB0Y6C5.

    Family and domain databases

    Gene3Di2.140.10.30. 1 hit.
    3.40.50.1820. 1 hit.
    InterProiIPR029058. AB_hydrolase.
    IPR001375. Peptidase_S9.
    IPR002469. Peptidase_S9B.
    [Graphical view]
    PfamiPF00930. DPPIV_N. 1 hit.
    PF00326. Peptidase_S9. 1 hit.
    [Graphical view]
    SUPFAMiSSF53474. SSF53474. 1 hit.
    ProtoNetiSearch...

    Publicationsi

    « Hide 'large scale' publications
    1. "Dipeptidyl-peptidase IV secreted by Aspergillus fumigatus, a fungus pathogenic to humans."
      Beauvais A., Monod M., Wyniger J., Debeaupuis J.P., Grouzmann E., Brakch N., Svab J., Hovanessian A.G., Latge J.P.
      Infect. Immun. 65:3042-3047(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], SUBCELLULAR LOCATION, FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: CEA10 / CBS 144.89 / FGSC A1163.

    Entry informationi

    Entry nameiDPP4_ASPFC
    AccessioniPrimary (citable) accession number: B0Y6C5
    Secondary accession number(s): O14425
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 5, 2010
    Last sequence update: April 8, 2008
    Last modified: June 24, 2015
    This is version 40 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.