ID   PDSA_ASPFC              Reviewed;         735 AA.
AC   B0Y5B4;
DT   22-FEB-2012, integrated into UniProtKB/Swiss-Prot.
DT   08-APR-2008, sequence version 1.
DT   13-SEP-2023, entry version 60.
DE   RecName: Full=Protostadienol synthase A;
DE            EC=5.4.99.32;
GN   Name=pdsA; ORFNames=AFUB_072030;
OS   Aspergillus fumigatus (strain CBS 144.89 / FGSC A1163 / CEA10) (Neosartorya
OS   fumigata).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC   Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus;
OC   Aspergillus subgen. Fumigati.
OX   NCBI_TaxID=451804;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CBS 144.89 / FGSC A1163 / CEA10;
RX   PubMed=18404212; DOI=10.1371/journal.pgen.1000046;
RA   Fedorova N.D., Khaldi N., Joardar V.S., Maiti R., Amedeo P., Anderson M.J.,
RA   Crabtree J., Silva J.C., Badger J.H., Albarraq A., Angiuoli S., Bussey H.,
RA   Bowyer P., Cotty P.J., Dyer P.S., Egan A., Galens K., Fraser-Liggett C.M.,
RA   Haas B.J., Inman J.M., Kent R., Lemieux S., Malavazi I., Orvis J.,
RA   Roemer T., Ronning C.M., Sundaram J.P., Sutton G., Turner G., Venter J.C.,
RA   White O.R., Whitty B.R., Youngman P., Wolfe K.H., Goldman G.H.,
RA   Wortman J.R., Jiang B., Denning D.W., Nierman W.C.;
RT   "Genomic islands in the pathogenic filamentous fungus Aspergillus
RT   fumigatus.";
RL   PLoS Genet. 4:E1000046-E1000046(2008).
CC   -!- FUNCTION: Protostadienol synthase which cyclizes (3S)-oxidosqualene to
CC       (17Z)-protosta-17(20),24-dien-3-beta-ol (protostadienol), the
CC       biosynthetic precursor of helvolic acid, a secondary metabolite which
CC       promotes virulence. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(S)-2,3-epoxysqualene = (17Z)-protosta-17(20),24-dien-3beta-
CC         ol; Xref=Rhea:RHEA:30987, ChEBI:CHEBI:15441, ChEBI:CHEBI:62457;
CC         EC=5.4.99.32;
CC   -!- SIMILARITY: Belongs to the terpene cyclase/mutase family.
CC       {ECO:0000305}.
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DR   EMBL; DS499598; EDP50863.1; -; Genomic_DNA.
DR   AlphaFoldDB; B0Y5B4; -.
DR   SMR; B0Y5B4; -.
DR   EnsemblFungi; EDP50863; EDP50863; AFUB_072030.
DR   VEuPathDB; FungiDB:AFUB_072030; -.
DR   HOGENOM; CLU_009074_2_1_1; -.
DR   PhylomeDB; B0Y5B4; -.
DR   Proteomes; UP000001699; Unassembled WGS sequence.
DR   GO; GO:0005811; C:lipid droplet; IEA:InterPro.
DR   GO; GO:0016866; F:intramolecular transferase activity; IEA:InterPro.
DR   GO; GO:0006694; P:steroid biosynthetic process; IEA:UniProtKB-KW.
DR   GO; GO:0016104; P:triterpenoid biosynthetic process; IEA:InterPro.
DR   CDD; cd02892; SQCY_1; 1.
DR   Gene3D; 1.50.10.20; -; 2.
DR   Gene3D; 6.20.120.20; -; 1.
DR   InterPro; IPR032696; SQ_cyclase_C.
DR   InterPro; IPR032697; SQ_cyclase_N.
DR   InterPro; IPR018333; Squalene_cyclase.
DR   InterPro; IPR008930; Terpenoid_cyclase/PrenylTrfase.
DR   NCBIfam; TIGR01787; squalene_cyclas; 1.
DR   PANTHER; PTHR11764:SF20; LANOSTEROL SYNTHASE; 1.
DR   PANTHER; PTHR11764; TERPENE CYCLASE/MUTASE FAMILY MEMBER; 1.
DR   Pfam; PF13243; SQHop_cyclase_C; 1.
DR   Pfam; PF13249; SQHop_cyclase_N; 1.
DR   SFLD; SFLDG01016; Prenyltransferase_Like_2; 1.
DR   SUPFAM; SSF48239; Terpenoid cyclases/Protein prenyltransferases; 2.
PE   3: Inferred from homology;
KW   Isomerase; Lipid biosynthesis; Lipid metabolism; Repeat;
KW   Steroid biosynthesis; Virulence.
FT   CHAIN           1..735
FT                   /note="Protostadienol synthase A"
FT                   /id="PRO_0000415496"
FT   REPEAT          132..173
FT                   /note="PFTB 1"
FT   REPEAT          490..531
FT                   /note="PFTB 2"
FT   REPEAT          567..607
FT                   /note="PFTB 3"
FT   REPEAT          616..663
FT                   /note="PFTB 4"
FT   ACT_SITE        463
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000250|UniProtKB:P48449"
SQ   SEQUENCE   735 AA;  83091 MW;  AA0AC92A8A6E36F8 CRC64;
     MATDSSMPGT VIGKAEFSDT KAASEFGTDL SRWRLNVDNG RHMWEYLESE DEARKRPQSF
     LEKYWLGLPY ELPARPRATC ALEAVENGWE FFKRLQTADG HWGCNDDGPL FVTSGMVIAR
     YIVGIPIDSH MKQEMCRYLL NVVNEDGGWG LFIQSPSTVF GTVMNYCMLR ILGLGPEHPA
     MAKARNTLHR LGSARATPTW GKFWLCVLGV YEWEGMVPLP PEPLLVPASL PFNPGKWWVH
     TRNVYISMSY LYGHRFSMPP NKLVQALRDE LYDIPYQQIN WPAQRTNVSA ADRLTDPTWI
     QRSFTSALTM YETFKIPFLR RRALNEALFQ IETETRNTHY LCIAPVSFAS NMLALYHAHG
     RDSHWIRGMR DRFIDPMWLC REGLAASGTN GTSLWDTALT VQATIDAGLA ARPENQAILR
     KALEFIDNSQ IREDPLGVHH VYRQPTRGAW PFSTRDQSYA VSDTTAEAVK VIVLLQRIEG
     FPSRISDERL QQAIDLILGM ENAGGGFSAY EPVRGPKFLE LLNITELYEN VMTDNLYPEC
     TSSVIMCLTT FAREYPTYRP RDIQACLSRS IDYLLRSQYP NGGWFASWGV CFTYATMFAL
     QGLACMGWNE SNCAACQRAC SFLLQHQNPD GGWGESLDTV RFKQYLPHPD GSQVTNTAYA
     VIGLLAARCG NHEAIRRGVA YLVKEQQDTG EWLPGPLEGV FAPPGGMRYP NYKFHFTLMA
     LGRYVAIHGN ECLAI
//