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Protein

Aspergillopepsin-1

Gene

pepA

Organism
Neosartorya fumigata (strain CEA10 / CBS 144.89 / FGSC A1163) (Aspergillus fumigatus)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Secreted aspartic endopeptidase that allows assimilation of proteinaceous substrates. The scissile peptide bond is attacked by a nucleophilic water molecule activated by two aspartic residues in the active site. Shows a broad primary substrate specificity. Favors hydrophobic residues at the P1 and P1' positions, but also accepts a lysine residue in the P1 position, leading to the activation of trypsinogen and chymotrypsinogen A.By similarity

Catalytic activityi

Hydrolysis of proteins with broad specificity. Generally favors hydrophobic residues in P1 and P1', but also accepts Lys in P1, which leads to activation of trypsinogen. Does not clot milk.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei102PROSITE-ProRule annotation1
Active sitei284PROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywordsi

Molecular functionAspartyl protease, Hydrolase, Protease
Biological processVirulence

Names & Taxonomyi

Protein namesi
Recommended name:
Aspergillopepsin-1Curated (EC:3.4.23.18By similarity)
Alternative name(s):
Aspartic protease pepA
Aspergillopepsin I
Aspergillopeptidase A
Gene namesi
Name:pepA
ORF Names:AFUB_061010
OrganismiNeosartorya fumigata (strain CEA10 / CBS 144.89 / FGSC A1163) (Aspergillus fumigatus)
Taxonomic identifieri451804 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
Proteomesi
  • UP000001699 Componenti: Unassembled WGS sequence

Subcellular locationi

  • Secreted By similarity

GO - Cellular componenti

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Protein family/group databases

Allergomei63. Asp f 10.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 20Sequence analysisAdd BLAST20
PropeptideiPRO_000040704321 – 70Activation peptideBy similarityAdd BLAST50
ChainiPRO_000040704471 – 395Aspergillopepsin-1Add BLAST325

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi320 ↔ 355PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Zymogen

Interactioni

Subunit structurei

Monomer.By similarity

Structurei

3D structure databases

ProteinModelPortaliB0Y1V8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini86 – 392Peptidase A1PROSITE-ProRule annotationAdd BLAST307

Sequence similaritiesi

Belongs to the peptidase A1 family.PROSITE-ProRule annotation
Contains 1 peptidase A1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

HOGENOMiHOG000167484.
OrthoDBiEOG092C49YE.
PhylomeDBiB0Y1V8.

Family and domain databases

Gene3Di2.40.70.10. 2 hits.
InterProiIPR001461. Aspartic_peptidase_A1.
IPR001969. Aspartic_peptidase_AS.
IPR033121. PEPTIDASE_A1.
IPR021109. Peptidase_aspartic_dom.
[Graphical view]
PANTHERiPTHR13683. PTHR13683. 2 hits.
PfamiPF00026. Asp. 1 hit.
[Graphical view]
PRINTSiPR00792. PEPSIN.
SUPFAMiSSF50630. SSF50630. 1 hit.
PROSITEiPS00141. ASP_PROTEASE. 2 hits.
PS51767. PEPTIDASE_A1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

B0Y1V8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVVFSKVTAV VVGLSTIVSA VPVVQPRKGF TINQVARPVT NKKTVNLPAV
60 70 80 90 100
YANALTKYGG TVPDSVKAAA SSGSAVTTPE QYDSEYLTPV KVGGTTLNLD
110 120 130 140 150
FDTGSADLWV FSSELSASQS SGHAIYKPSA NAQKLNGYTW KIQYGDGSSA
160 170 180 190 200
SGDVYKDTVT VGGVTAQSQA VEAASHISSQ FVQDKDNDGL LGLAFSSINT
210 220 230 240 250
VSPRPQTTFF DTVKSQLDSP LFAVTLKYHA PGTYDFGYID NSKFQGELTY
260 270 280 290 300
TDVDSSQGFW MFTADGYGVG NGAPNSNSIS GIADTGTTLL LLDDSVVADY
310 320 330 340 350
YRQVSGAKNS NQYGGYVFPC STKLPSFTTV IGGYNAVVPG EYINYAPVTD
360 370 380 390
GSSTCYGGIQ SNSGLGFSIF GDIFLKSQYV VFDSQGPRLG FAPQA
Length:395
Mass (Da):41,613
Last modified:April 8, 2008 - v1
Checksum:iF3D1CCC0C6814BA9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DS499597 Genomic DNA. Translation: EDP52067.1.

Genome annotation databases

EnsemblFungiiCADAFUBT00006081; CADAFUBP00005960; CADAFUBG00006081.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DS499597 Genomic DNA. Translation: EDP52067.1.

3D structure databases

ProteinModelPortaliB0Y1V8.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

Allergomei63. Asp f 10.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiCADAFUBT00006081; CADAFUBP00005960; CADAFUBG00006081.

Phylogenomic databases

HOGENOMiHOG000167484.
OrthoDBiEOG092C49YE.
PhylomeDBiB0Y1V8.

Family and domain databases

Gene3Di2.40.70.10. 2 hits.
InterProiIPR001461. Aspartic_peptidase_A1.
IPR001969. Aspartic_peptidase_AS.
IPR033121. PEPTIDASE_A1.
IPR021109. Peptidase_aspartic_dom.
[Graphical view]
PANTHERiPTHR13683. PTHR13683. 2 hits.
PfamiPF00026. Asp. 1 hit.
[Graphical view]
PRINTSiPR00792. PEPSIN.
SUPFAMiSSF50630. SSF50630. 1 hit.
PROSITEiPS00141. ASP_PROTEASE. 2 hits.
PS51767. PEPTIDASE_A1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPEPA_ASPFC
AccessioniPrimary (citable) accession number: B0Y1V8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 5, 2011
Last sequence update: April 8, 2008
Last modified: January 18, 2017
This is version 45 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.