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Protein

Probable beta-galactosidase B

Gene

lacB

Organism
Neosartorya fumigata (strain CEA10 / CBS 144.89 / FGSC A1163) (Aspergillus fumigatus)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Cleaves beta-linked terminal galactosyl residues from gangliosides, glycoproteins, and glycosaminoglycans.By similarity

Catalytic activityi

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei90SubstrateBy similarity1
Binding sitei135SubstrateBy similarity1
Binding sitei136Substrate; via amide nitrogenBy similarity1
Binding sitei137SubstrateBy similarity1
Binding sitei195SubstrateBy similarity1
Active sitei196Proton donorSequence analysis1
Binding sitei265SubstrateBy similarity1
Active sitei308NucleophileSequence analysis1
Binding sitei373SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Polysaccharide degradation

Names & Taxonomyi

Protein namesi
Recommended name:
Probable beta-galactosidase B (EC:3.2.1.23)
Alternative name(s):
Lactase B
Gene namesi
Name:lacB
ORF Names:AFUB_016080
OrganismiNeosartorya fumigata (strain CEA10 / CBS 144.89 / FGSC A1163) (Aspergillus fumigatus)
Taxonomic identifieri451804 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
Proteomesi
  • UP000001699 Componenti: Unassembled WGS sequence

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 20Sequence analysisAdd BLAST20
ChainiPRO_000039522321 – 1015Probable beta-galactosidase BAdd BLAST995

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi23N-linked (GlcNAc...)Sequence analysis1
Glycosylationi99N-linked (GlcNAc...)Sequence analysis1
Glycosylationi100N-linked (GlcNAc...)Sequence analysis1
Glycosylationi172N-linked (GlcNAc...)Sequence analysis1
Glycosylationi211N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi271 ↔ 324By similarity
Glycosylationi411N-linked (GlcNAc...)Sequence analysis1
Glycosylationi456N-linked (GlcNAc...)Sequence analysis1
Glycosylationi554N-linked (GlcNAc...)Sequence analysis1
Glycosylationi679N-linked (GlcNAc...)Sequence analysis1
Glycosylationi735N-linked (GlcNAc...)Sequence analysis1
Glycosylationi775N-linked (GlcNAc...)Sequence analysis1
Glycosylationi821N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein

Structurei

3D structure databases

ProteinModelPortaliB0XNY2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 35 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

HOGENOMiHOG000181922.
OrthoDBiEOG092C0SLI.
PhylomeDBiB0XNY2.

Family and domain databases

Gene3Di2.102.20.10. 1 hit.
2.60.120.260. 2 hits.
2.60.390.10. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR018954. Betagal_dom2.
IPR025972. BetaGal_dom3.
IPR025300. BetaGal_jelly_roll_dom.
IPR008979. Galactose-bd-like.
IPR031330. Gly_Hdrlase_35_cat.
IPR013781. Glyco_hydro_catalytic_dom.
IPR001944. Glycoside_Hdrlase_35.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR23421. PTHR23421. 2 hits.
PfamiPF10435. BetaGal_dom2. 1 hit.
PF13363. BetaGal_dom3. 1 hit.
PF13364. BetaGal_dom4_5. 2 hits.
PF01301. Glyco_hydro_35. 1 hit.
[Graphical view]
PRINTSiPR00742. GLHYDRLASE35.
SMARTiSM01029. BetaGal_dom2. 1 hit.
[Graphical view]
SUPFAMiSSF117100. SSF117100. 1 hit.
SSF49785. SSF49785. 2 hits.
SSF51445. SSF51445. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

B0XNY2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAHIYRLLLL LLSNLWFSTA AQNQSETEWP LHDNGLSKVV QWDHYSFQVN
60 70 80 90 100
GQRIFIFSGE FHYWRIPVPE LWRDILEKVK ATGFTAFAFY SSWAYHAPNN
110 120 130 140 150
STVDFSTGAR DITPIFELAK ELGMYMIVRP GPYVNAEASA GGFPLWLMTG
160 170 180 190 200
EYGSLRNDDP RYTAAWTPYF ANMSQITSKY QVTDGHNTLV YQIENEYGQQ
210 220 230 240 250
WIGDPKNRNP NKTAVAYMEL LEASARENGI TVPLTSNDPN MNSKSWGSDW
260 270 280 290 300
SNAGGNVDVA GLDSYPSCWT CDVSQCTSTN GEYVPYKVID YYDYFQEVQP
310 320 330 340 350
TLPSFMPEFQ GGSYNPWAGP EGGCPQDTSA EFANLFYRWN IGQRVTAMSL
360 370 380 390 400
YMLYGGTNWG AIAAPVTATS YDYSAPISED RSIGAKYSET KLLALFTRTA
410 420 430 440 450
KDLTMTEAIG NGTQYTTNTA VRAFELRNPQ TNAGFYVTFH TDTTVGGNQA
460 470 480 490 500
FKLHVNTSVG ALTVPKNEGL IQLNGHQSKI IVTDFTLGKR TLLYSTAEVL
510 520 530 540 550
TYAVFENRPT LVLWVPTGES GEFAIKGAKS GKVENGDGCS GIKFKREKDY
560 570 580 590 600
LVVNFSQAKG LSVLRLDNGV RVVLLDKAAA YRFWAPALTD DPNVQETETV
610 620 630 640 650
LVHGPYLVRS ASISKTTLAL RGDSVEKTTL EIFAPHSVRK ITWNGKEVQT
660 670 680 690 700
SHTPYGSLKA TLAAPPDIKL PALTSWRSND SLPERLPSYD DSGPAWIEAN
710 720 730 740 750
HMTTSNPSPP ATFPVLYADE YGFHNGVRLW RGYFNGSASG VFLNIQGGSA
760 770 780 790 800
FGWSAWLNGH FLDSHLGTAT TSQANKTLTF PSSILNPTEN VLLIVHDDTG
810 820 830 840 850
HDQTTGALNP RGILEARLLS NDTSSPPPEF THWRLAGTAG GESNLDPIRG
860 870 880 890 900
VFNEDGLFAE RMGWHLPGFD DSAWTSENSA TSASSALSFT GATVRFFRSV
910 920 930 940 950
VPLNIPAGLD VSISFVLSTP TAAPKGYRAQ LFVNGYQYGR YNPHIGNQVV
960 970 980 990 1000
FPVPPGILDY QGDNTIGLAV WAQTEEGAGI QVDWKVNYVA DSSLSVAGFG
1010
KGLRPGWTEE RLKFA
Length:1,015
Mass (Da):111,681
Last modified:April 8, 2008 - v1
Checksum:iD9EB8677F99A13A8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DS499594 Genomic DNA. Translation: EDP56886.1.

Genome annotation databases

EnsemblFungiiCADAFUBT00001613; CADAFUBP00001576; CADAFUBG00001613.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DS499594 Genomic DNA. Translation: EDP56886.1.

3D structure databases

ProteinModelPortaliB0XNY2.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiCADAFUBT00001613; CADAFUBP00001576; CADAFUBG00001613.

Phylogenomic databases

HOGENOMiHOG000181922.
OrthoDBiEOG092C0SLI.
PhylomeDBiB0XNY2.

Family and domain databases

Gene3Di2.102.20.10. 1 hit.
2.60.120.260. 2 hits.
2.60.390.10. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR018954. Betagal_dom2.
IPR025972. BetaGal_dom3.
IPR025300. BetaGal_jelly_roll_dom.
IPR008979. Galactose-bd-like.
IPR031330. Gly_Hdrlase_35_cat.
IPR013781. Glyco_hydro_catalytic_dom.
IPR001944. Glycoside_Hdrlase_35.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR23421. PTHR23421. 2 hits.
PfamiPF10435. BetaGal_dom2. 1 hit.
PF13363. BetaGal_dom3. 1 hit.
PF13364. BetaGal_dom4_5. 2 hits.
PF01301. Glyco_hydro_35. 1 hit.
[Graphical view]
PRINTSiPR00742. GLHYDRLASE35.
SMARTiSM01029. BetaGal_dom2. 1 hit.
[Graphical view]
SUPFAMiSSF117100. SSF117100. 1 hit.
SSF49785. SSF49785. 2 hits.
SSF51445. SSF51445. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiBGALB_ASPFC
AccessioniPrimary (citable) accession number: B0XNY2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 13, 2010
Last sequence update: April 8, 2008
Last modified: September 7, 2016
This is version 44 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.