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B0WSB8 (TRMB_CULQU) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 42. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA (guanine-N(7)-)-methyltransferase

EC=2.1.1.33
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase
Gene names
ORF Names:CPIJ009694
OrganismCulex quinquefasciatus (Southern house mosquito) (Culex pungens) [Complete proteome]
Taxonomic identifier7176 [NCBI]
Taxonomic lineageEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraNematoceraCulicoideaCulicidaeCulicinaeCuliciniCulexCulex

Protein attributes

Sequence length239 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity. HAMAP-Rule MF_03055

Catalytic activity

S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA. HAMAP-Rule MF_03055

Pathway

tRNA modification; N(7)-methylguanine-tRNA biosynthesis. HAMAP-Rule MF_03055

Subcellular location

Nucleus By similarity HAMAP-Rule MF_03055.

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.

Ontologies

Keywords
   Biological processtRNA processing
   Cellular componentNucleus
   LigandRNA-binding
S-adenosyl-L-methionine
tRNA-binding
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Cellular_componentnucleus

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functiontRNA (guanine-N7-)-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

tRNA binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 239239tRNA (guanine-N(7)-)-methyltransferase HAMAP-Rule MF_03055
PRO_0000370567

Regions

Region87 – 882S-adenosyl-L-methionine binding By similarity
Region120 – 1212S-adenosyl-L-methionine binding By similarity
Region218 – 2203S-adenosyl-L-methionine binding By similarity

Sites

Active site1431 By similarity
Binding site641S-adenosyl-L-methionine; via carbonyl oxygen By similarity
Binding site1401S-adenosyl-L-methionine; via carbonyl oxygen By similarity

Sequences

Sequence LengthMass (Da)Tools
B0WSB8 [UniParc].

Last modified April 8, 2008. Version 1.
Checksum: 56ED500166BECCC3

FASTA23927,999
        10         20         30         40         50         60 
MEVDSKKSSD VVTKLPQKRF YRQRAHSNPI ADHSFQYPAH PDEYEWSQHY PDIGDRRVAF 

        70         80         90        100        110        120 
ADIGCGYGGF LVTLGEIYPD KFAVGMEIRV KVSDYVMDRI DALRKLHEGQ YRNVACIRTN 

       130        140        150        160        170        180 
AMKYLTNFFH KAQLEKMFFL YPDPHFKKAK HKWRIINSAL LSEYSYVLRK GGLIYTVTDV 

       190        200        210        220        230 
RDLHEWMCKH IEQHPAFERL SEAEVEADIL SEKLLDSSEE GKKVTRNKGD KFVAIFRRV 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
DS232068 Genomic DNA. Translation: EDS33756.1.
RefSeqXP_001851677.1. XM_001851626.1.
UniGeneCpi.5362.

3D structure databases

ProteinModelPortalB0WSB8.
SMRB0WSB8. Positions 37-239.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING7176.CPIJ009694-PA.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaCPIJ009694-RA; CPIJ009694-PA; CPIJ009694.
GeneID6042495.
KEGGcqu:CpipJ_CPIJ009694.
VectorBaseCPIJ009694. Culex quinquefasciatus.

Phylogenomic databases

eggNOGCOG0220.
HOGENOMHOG000260965.
KOK03439.
OMARAHSNPI.
OrthoDBEOG7PS1GC.
PhylomeDBB0WSB8.

Enzyme and pathway databases

UniPathwayUPA00989.

Family and domain databases

Gene3D3.40.50.150. 1 hit.
HAMAPMF_03055. tRNA_methyltr_TrmB_euk.
InterProIPR029063. SAM-dependent_MTases-like.
IPR025763. Trm8_euk.
IPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMSSF53335. SSF53335. 1 hit.
TIGRFAMsTIGR00091. TIGR00091. 1 hit.
PROSITEPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTRMB_CULQU
AccessionPrimary (citable) accession number: B0WSB8
Entry history
Integrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: April 8, 2008
Last modified: June 11, 2014
This is version 42 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways