B0WSB8 (TRMB_CULQU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
tRNA (guanine-N(7)-)-methyltransferase
EC=2.1.1.33

Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase

Gene names

ORF Names:

CPIJ009694

Organism

Culex quinquefasciatus (Southern house mosquito) (Culex pungens) [Complete proteome]

Taxonomic identifier

7176 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Panarthropoda › Arthropoda › Mandibulata › Pancrustacea › Hexapoda › Insecta › Dicondylia › Pterygota › Neoptera › Endopterygota › Diptera › Nematocera › Culicimorpha › Culicoidea › Culicidae › Culicinae › Culicini › Culex › Culex › Culex pipiens complex

Protein attributes

Sequence length	239 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity.
Catalytic activity	S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA.
Pathway	tRNA modification; N(7)-methylguanine-tRNA biosynthesis.
Subcellular location	Nucleus By similarity.
Sequence similarities	Belongs to the methyltransferase superfamily. TrmB family.

Ontologies

Keywords
Biological process	tRNA processing
Cellular component	Nucleus
Ligand	RNA-binding S-adenosyl-L-methionine tRNA-binding
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	RNA (guanine-N7)-methylation Inferred from electronic annotation. Source: GOC
Cellular_component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	tRNA (guanine-N7-)-methyltransferase activity Inferred from electronic annotation. Source: EC tRNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 239	239	tRNA (guanine-N(7)-)-methyltransferase		PRO_0000370567
Regions
	Region	87 – 88	2	S-adenosyl-L-methionine binding By similarity
	Region	120 – 121	2	S-adenosyl-L-methionine binding By similarity
	Region	218 – 220	3	S-adenosyl-L-methionine binding By similarity
Sites
	Active site	143	1	By similarity
	Binding site	64	1	S-adenosyl-L-methionine; via carbonyl oxygen By similarity
	Binding site	140	1	S-adenosyl-L-methionine; via carbonyl oxygen By similarity

Sequences

	Sequence		Length	Mass (Da)
	B0WSB8 [UniParc]. Last modified April 8, 2008. Version 1. Checksum: 56ED500166BECCC3	FASTA	239	27,999
	10 20 30 40 50 60 MEVDSKKSSD VVTKLPQKRF YRQRAHSNPI ADHSFQYPAH PDEYEWSQHY PDIGDRRVAF 70 80 90 100 110 120 ADIGCGYGGF LVTLGEIYPD KFAVGMEIRV KVSDYVMDRI DALRKLHEGQ YRNVACIRTN 130 140 150 160 170 180 AMKYLTNFFH KAQLEKMFFL YPDPHFKKAK HKWRIINSAL LSEYSYVLRK GGLIYTVTDV 190 200 210 220 230 RDLHEWMCKH IEQHPAFERL SEAEVEADIL SEKLLDSSEE GKKVTRNKGD KFVAIFRRV

References

[1]

"Annotation of Culex pipiens quinquefasciatus." The Broad Institute Genome Sequencing Platform Atkinson P.W., Hemingway J., Christensen B.M., Higgs S., Kodira C.D., Hannick L.I., Megy K., O'Leary S.B., Pearson M., Haas B.J., Mauceli E., Wortman J.R., Lee N.H., Guigo R., Stanke M., Alvarado L., Amedeo P., Antoine C.H. , Arensburger P., Bidwell S.L., Crawford M., Camaro F., Devon K., Engels R., Hammond M., Howarth C., Koehrsen M., Lawson D., Montgomery P., Nene V., Nusbaum C., Puiu D., Romero-Severson J., Severson D.W., Shumway M., Sisk P., Stolte C., Zeng Q., Eisenstadt E., Fraser-Liggett C.M., Strausberg R., Galagan J., Birren B., Collins F.H. Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: JHB.

Cross-references

Sequence databases
EMBL GenBank DDBJ	DS232068 Genomic DNA. Translation: EDS33756.1.
RefSeq	XP_001851677.1. XM_001851626.1.
UniGene	Cpi.5362.
3D structure databases
ProteinModelPortal	B0WSB8.
SMR	B0WSB8. Positions 37-239.
ModBase	Search...
Protein-protein interaction databases
STRING	7176.CPIJ009694-PA.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	CPIJ009694-RA; CPIJ009694-PA; CPIJ009694.
GeneID	6042495.
KEGG	cqu:CpipJ_CPIJ009694.
VectorBase	CPIJ009694. Culex quinquefasciatus.
Phylogenomic databases
eggNOG	COG0220.
HOGENOM	HOG000260965.
KO	K03439.
OMA	RAHSNPI.
OrthoDB	EOG4PC887.
PhylomeDB	B0WSB8.
Enzyme and pathway databases
UniPathway	UPA00989.
Family and domain databases
InterPro	IPR003358. tRNA_(Gua-N-7)_MeTrfase. IPR025763. tRNA_(Gua-N-7)_MeTrfase_euk. [Graphical view]
Pfam	PF02390. Methyltransf_4. 1 hit. [Graphical view]
TIGRFAMs	TIGR00091. TIGR00091. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TRMB_CULQU

Accession

Primary (citable) accession number: B0WSB8

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 5, 2009
Last sequence update:	April 8, 2008
Last modified:	April 3, 2013
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)