Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

CPIJ009694

Organism
Culex quinquefasciatus (Southern house mosquito) (Culex pungens)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of N7-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N7-methylguanine(46) in tRNA.UniRule annotation

Pathwayi: N(7)-methylguanine-tRNA biosynthesis

This protein is involved in the pathway N(7)-methylguanine-tRNA biosynthesis, which is part of tRNA modification.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway N(7)-methylguanine-tRNA biosynthesis and in tRNA modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei64S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation1
Binding sitei140S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation1
Active sitei143UniRule annotation1

GO - Molecular functioni

Keywordsi

Molecular functionMethyltransferase, RNA-binding, Transferase, tRNA-binding
Biological processtRNA processing
LigandS-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00989.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
tRNA(m7G46)-methyltransferaseUniRule annotation
Gene namesi
ORF Names:CPIJ009694
OrganismiCulex quinquefasciatus (Southern house mosquito) (Culex pungens)
Taxonomic identifieri7176 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraNematoceraCulicoideaCulicidaeCulicinaeCuliciniCulexCulex
Proteomesi
  • UP000002320 Componenti: Partially assembled WGS sequence

Subcellular locationi

  • Nucleus UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003705671 – 239tRNA (guanine-N(7)-)-methyltransferaseAdd BLAST239

Interactioni

Protein-protein interaction databases

STRINGi7176.CPIJ009694-PA.

Structurei

3D structure databases

ProteinModelPortaliB0WSB8.
SMRiB0WSB8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni87 – 88S-adenosyl-L-methionine bindingUniRule annotation2
Regioni120 – 121S-adenosyl-L-methionine bindingUniRule annotation2
Regioni218 – 220S-adenosyl-L-methionine bindingUniRule annotation3

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

Phylogenomic databases

eggNOGiKOG3115. Eukaryota.
COG0220. LUCA.
HOGENOMiHOG000260965.
InParanoidiB0WSB8.
KOiK03439.
OMAiIGMEIRV.
OrthoDBiEOG091G0IQ7.
PhylomeDBiB0WSB8.

Family and domain databases

HAMAPiMF_03055. tRNA_methyltr_TrmB_euk. 1 hit.
InterProiView protein in InterPro
IPR029063. SAM-dependent_MTases.
IPR025763. Trm8_euk.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
PANTHERiPTHR23417. PTHR23417. 1 hit.
PTHR23417:SF20. PTHR23417:SF20. 1 hit.
PfamiView protein in Pfam
PF02390. Methyltransf_4. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiView protein in PROSITE
PS51625. SAM_MT_TRMB. 1 hit.

Sequencei

Sequence statusi: Complete.

B0WSB8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEVDSKKSSD VVTKLPQKRF YRQRAHSNPI ADHSFQYPAH PDEYEWSQHY
60 70 80 90 100
PDIGDRRVAF ADIGCGYGGF LVTLGEIYPD KFAVGMEIRV KVSDYVMDRI
110 120 130 140 150
DALRKLHEGQ YRNVACIRTN AMKYLTNFFH KAQLEKMFFL YPDPHFKKAK
160 170 180 190 200
HKWRIINSAL LSEYSYVLRK GGLIYTVTDV RDLHEWMCKH IEQHPAFERL
210 220 230
SEAEVEADIL SEKLLDSSEE GKKVTRNKGD KFVAIFRRV
Length:239
Mass (Da):27,999
Last modified:April 8, 2008 - v1
Checksum:i56ED500166BECCC3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DS232068 Genomic DNA. Translation: EDS33756.1.
RefSeqiXP_001851677.1. XM_001851626.1.
UniGeneiCpi.5362.

Genome annotation databases

EnsemblMetazoaiCPIJ009694-RA; CPIJ009694-PA; CPIJ009694.
GeneIDi6042495.
KEGGicqu:CpipJ_CPIJ009694.
VectorBaseiCPIJ009694-RA; CPIJ009694-PA; CPIJ009694.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiTRMB_CULQU
AccessioniPrimary (citable) accession number: B0WSB8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: April 8, 2008
Last modified: June 7, 2017
This is version 57 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families