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B0WSB8

- TRMB_CULQU

UniProt

B0WSB8 - TRMB_CULQU

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Protein
tRNA (guanine-N(7)-)-methyltransferase
Gene
CPIJ009694
Organism
Culex quinquefasciatus (Southern house mosquito) (Culex pungens)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei64 – 641S-adenosyl-L-methionine; via carbonyl oxygen By similarity
Binding sitei140 – 1401S-adenosyl-L-methionine; via carbonyl oxygen By similarity
Active sitei143 – 1431 By similarity

GO - Molecular functioni

  1. tRNA (guanine-N7-)-methyltransferase activity Source: UniProtKB-HAMAP
  2. tRNA binding Source: UniProtKB-HAMAP
Complete GO annotation...

GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Biological processi

    tRNA processing

    Keywords - Ligandi

    RNA-binding, S-adenosyl-L-methionine, tRNA-binding

    Enzyme and pathway databases

    UniPathwayiUPA00989.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    tRNA (guanine-N(7)-)-methyltransferase (EC:2.1.1.33)
    Alternative name(s):
    tRNA (guanine(46)-N(7))-methyltransferase
    tRNA(m7G46)-methyltransferase
    Gene namesi
    ORF Names:CPIJ009694
    OrganismiCulex quinquefasciatus (Southern house mosquito) (Culex pungens)
    Taxonomic identifieri7176 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraNematoceraCulicoideaCulicidaeCulicinaeCuliciniCulexCulex
    ProteomesiUP000002320: Partially assembled WGS sequence

    Subcellular locationi

    Nucleus By similarity UniRule annotation

    GO - Cellular componenti

    1. nucleus Source: UniProtKB-SubCell
    Complete GO annotation...

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 239239tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation
    PRO_0000370567Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi7176.CPIJ009694-PA.

    Structurei

    3D structure databases

    ProteinModelPortaliB0WSB8.
    SMRiB0WSB8. Positions 37-239.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni87 – 882S-adenosyl-L-methionine binding By similarity
    Regioni120 – 1212S-adenosyl-L-methionine binding By similarity
    Regioni218 – 2203S-adenosyl-L-methionine binding By similarity

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0220.
    HOGENOMiHOG000260965.
    KOiK03439.
    OMAiRAHSNPI.
    OrthoDBiEOG7PS1GC.
    PhylomeDBiB0WSB8.

    Family and domain databases

    Gene3Di3.40.50.150. 1 hit.
    HAMAPiMF_03055. tRNA_methyltr_TrmB_euk.
    InterProiIPR029063. SAM-dependent_MTases-like.
    IPR025763. Trm8_euk.
    IPR003358. tRNA_(Gua-N-7)_MeTrfase.
    [Graphical view]
    PfamiPF02390. Methyltransf_4. 1 hit.
    [Graphical view]
    SUPFAMiSSF53335. SSF53335. 1 hit.
    TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
    PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    B0WSB8-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEVDSKKSSD VVTKLPQKRF YRQRAHSNPI ADHSFQYPAH PDEYEWSQHY    50
    PDIGDRRVAF ADIGCGYGGF LVTLGEIYPD KFAVGMEIRV KVSDYVMDRI 100
    DALRKLHEGQ YRNVACIRTN AMKYLTNFFH KAQLEKMFFL YPDPHFKKAK 150
    HKWRIINSAL LSEYSYVLRK GGLIYTVTDV RDLHEWMCKH IEQHPAFERL 200
    SEAEVEADIL SEKLLDSSEE GKKVTRNKGD KFVAIFRRV 239
    Length:239
    Mass (Da):27,999
    Last modified:April 8, 2008 - v1
    Checksum:i56ED500166BECCC3
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    DS232068 Genomic DNA. Translation: EDS33756.1.
    RefSeqiXP_001851677.1. XM_001851626.1.
    UniGeneiCpi.5362.

    Genome annotation databases

    EnsemblMetazoaiCPIJ009694-RA; CPIJ009694-PA; CPIJ009694.
    GeneIDi6042495.
    KEGGicqu:CpipJ_CPIJ009694.
    VectorBaseiCPIJ009694. Culex quinquefasciatus.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    DS232068 Genomic DNA. Translation: EDS33756.1 .
    RefSeqi XP_001851677.1. XM_001851626.1.
    UniGenei Cpi.5362.

    3D structure databases

    ProteinModelPortali B0WSB8.
    SMRi B0WSB8. Positions 37-239.
    ModBasei Search...

    Protein-protein interaction databases

    STRINGi 7176.CPIJ009694-PA.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblMetazoai CPIJ009694-RA ; CPIJ009694-PA ; CPIJ009694 .
    GeneIDi 6042495.
    KEGGi cqu:CpipJ_CPIJ009694.
    VectorBasei CPIJ009694. Culex quinquefasciatus.

    Phylogenomic databases

    eggNOGi COG0220.
    HOGENOMi HOG000260965.
    KOi K03439.
    OMAi RAHSNPI.
    OrthoDBi EOG7PS1GC.
    PhylomeDBi B0WSB8.

    Enzyme and pathway databases

    UniPathwayi UPA00989 .

    Family and domain databases

    Gene3Di 3.40.50.150. 1 hit.
    HAMAPi MF_03055. tRNA_methyltr_TrmB_euk.
    InterProi IPR029063. SAM-dependent_MTases-like.
    IPR025763. Trm8_euk.
    IPR003358. tRNA_(Gua-N-7)_MeTrfase.
    [Graphical view ]
    Pfami PF02390. Methyltransf_4. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53335. SSF53335. 1 hit.
    TIGRFAMsi TIGR00091. TIGR00091. 1 hit.
    PROSITEi PS51625. SAM_MT_TRMB. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: JHB.

    Entry informationi

    Entry nameiTRMB_CULQU
    AccessioniPrimary (citable) accession number: B0WSB8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 5, 2009
    Last sequence update: April 8, 2008
    Last modified: June 11, 2014
    This is version 42 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3

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