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B0WAU6

- LIAS_CULQU

UniProt

B0WAU6 - LIAS_CULQU

Protein

Lipoyl synthase, mitochondrial

Gene

CPIJ004250

Organism
Culex quinquefasciatus (Southern house mosquito) (Culex pungens)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 40 (01 Oct 2014)
      Sequence version 1 (08 Apr 2008)
      Previous versions | rss
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    Functioni

    Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives.UniRule annotation

    Catalytic activityi

    Protein N(6)-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.

    Cofactori

    Binds 2 4Fe-4S clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi104 – 1041Iron-sulfur 1 (4Fe-4S)UniRule annotation
    Metal bindingi109 – 1091Iron-sulfur 1 (4Fe-4S)UniRule annotation
    Metal bindingi115 – 1151Iron-sulfur 1 (4Fe-4S)UniRule annotation
    Metal bindingi135 – 1351Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi139 – 1391Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi142 – 1421Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation

    GO - Molecular functioni

    1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
    2. lipoate synthase activity Source: UniProtKB-HAMAP
    3. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. protein lipoylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Transferase

    Keywords - Ligandi

    4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

    Enzyme and pathway databases

    UniPathwayiUPA00538; UER00593.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Lipoyl synthase, mitochondrialUniRule annotation (EC:2.8.1.8UniRule annotation)
    Alternative name(s):
    Lipoate synthaseUniRule annotation
    Short name:
    LSUniRule annotation
    Short name:
    Lip-synUniRule annotation
    Lipoic acid synthaseUniRule annotation
    Gene namesi
    ORF Names:CPIJ004250
    OrganismiCulex quinquefasciatus (Southern house mosquito) (Culex pungens)
    Taxonomic identifieri7176 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraNematoceraCulicoideaCulicidaeCulicinaeCuliciniCulexCulex
    ProteomesiUP000002320: Partially assembled WGS sequence

    Subcellular locationi

    Mitochondrion UniRule annotation

    GO - Cellular componenti

    1. mitochondrion Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Mitochondrion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini? – 380Lipoyl synthase, mitochondrialPRO_0000398215
    Transit peptidei1 – ?MitochondrionUniRule annotation

    Interactioni

    Protein-protein interaction databases

    STRINGi7176.CPIJ004250-PA.

    Structurei

    3D structure databases

    ProteinModelPortaliB0WAU6.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the radical SAM superfamily. Lipoyl synthase family.UniRule annotation

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG0320.
    HOGENOMiHOG000235998.
    KOiK03644.
    OMAiCKEGMIT.
    OrthoDBiEOG7P2XS7.
    PhylomeDBiB0WAU6.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    HAMAPiMF_00206. Lipoyl_synth.
    InterProiIPR013785. Aldolase_TIM.
    IPR006638. Elp3/MiaB/NifB.
    IPR003698. Lipoyl_synth.
    IPR007197. rSAM.
    [Graphical view]
    PANTHERiPTHR10949. PTHR10949. 1 hit.
    PfamiPF04055. Radical_SAM. 1 hit.
    [Graphical view]
    PIRSFiPIRSF005963. Lipoyl_synth. 1 hit.
    SMARTiSM00729. Elp3. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00510. lipA. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    B0WAU6-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKYFFSLPFP KVHTTCKHTA AATASQPLQK IRERLESGPG FQEFVQNPSY    50
    NREDWSAYEG KLKREKGEQD RLRLPPWLKT KIPMGKNFSR IKDQLRELKL 100
    ATVCEEAKCP NIGECWGGGE HGTQTATIML MGDTCTRGCR FCSVKTARAP 150
    PPLDPDEPKK TASAIASWGL DYIVLTSVDR DDLPDGGSNH IAETIREIKR 200
    QNPRIFVECL APDFRGNLDC IRTVATSGLD VYAHNIETVE SLTPFVRDRR 250
    AEYRQSLKCL ASVKEINPNM VTKTSIMLGL GETDEQVEQT MKDLRAVGVD 300
    CLTLGQYMQP TKRHLKVIEY VTPEKFKHWE TRGNELGFLY TASGPLVRSS 350
    YKAGEFFITS ILKNRAAAAA EEATATKPSE 380
    Length:380
    Mass (Da):42,657
    Last modified:April 8, 2008 - v1
    Checksum:i0DAB0709245B4EF8
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    DS231875 Genomic DNA. Translation: EDS41812.1.
    RefSeqiXP_001845830.1. XM_001845778.1.

    Genome annotation databases

    EnsemblMetazoaiCPIJ004250-RA; CPIJ004250-PA; CPIJ004250.
    GeneIDi6035696.
    KEGGicqu:CpipJ_CPIJ004250.
    VectorBaseiCPIJ004250. Culex quinquefasciatus.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    DS231875 Genomic DNA. Translation: EDS41812.1 .
    RefSeqi XP_001845830.1. XM_001845778.1.

    3D structure databases

    ProteinModelPortali B0WAU6.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 7176.CPIJ004250-PA.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblMetazoai CPIJ004250-RA ; CPIJ004250-PA ; CPIJ004250 .
    GeneIDi 6035696.
    KEGGi cqu:CpipJ_CPIJ004250.
    VectorBasei CPIJ004250. Culex quinquefasciatus.

    Phylogenomic databases

    eggNOGi COG0320.
    HOGENOMi HOG000235998.
    KOi K03644.
    OMAi CKEGMIT.
    OrthoDBi EOG7P2XS7.
    PhylomeDBi B0WAU6.

    Enzyme and pathway databases

    UniPathwayi UPA00538 ; UER00593 .

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    HAMAPi MF_00206. Lipoyl_synth.
    InterProi IPR013785. Aldolase_TIM.
    IPR006638. Elp3/MiaB/NifB.
    IPR003698. Lipoyl_synth.
    IPR007197. rSAM.
    [Graphical view ]
    PANTHERi PTHR10949. PTHR10949. 1 hit.
    Pfami PF04055. Radical_SAM. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF005963. Lipoyl_synth. 1 hit.
    SMARTi SM00729. Elp3. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00510. lipA. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: JHB.

    Entry informationi

    Entry nameiLIAS_CULQU
    AccessioniPrimary (citable) accession number: B0WAU6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 5, 2010
    Last sequence update: April 8, 2008
    Last modified: October 1, 2014
    This is version 40 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3