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B0WAU6

- LIAS_CULQU

UniProt

B0WAU6 - LIAS_CULQU

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Protein
Lipoyl synthase, mitochondrial
Gene
CPIJ004250
Organism
Culex quinquefasciatus (Southern house mosquito) (Culex pungens)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives By similarity.UniRule annotation

Catalytic activityi

Protein N(6)-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Binds 2 4Fe-4S clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi104 – 1041Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi109 – 1091Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi115 – 1151Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi135 – 1351Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi139 – 1391Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi142 – 1421Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
  2. lipoate synthase activity Source: UniProtKB-HAMAP
  3. metal ion binding Source: UniProtKB-KW
Complete GO annotation...

GO - Biological processi

  1. protein lipoylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00538; UER00593.

Names & Taxonomyi

Protein namesi
Recommended name:
Lipoyl synthase, mitochondrial (EC:2.8.1.8)
Alternative name(s):
Lipoate synthase
Short name:
LS
Short name:
Lip-syn
Lipoic acid synthase
Gene namesi
ORF Names:CPIJ004250
OrganismiCulex quinquefasciatus (Southern house mosquito) (Culex pungens)
Taxonomic identifieri7176 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraNematoceraCulicoideaCulicidaeCulicinaeCuliciniCulexCulex
ProteomesiUP000002320: Partially assembled WGS sequence

Subcellular locationi

Mitochondrion By similarity UniRule annotation

GO - Cellular componenti

  1. mitochondrion Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini? – 380Lipoyl synthase, mitochondrialUniRule annotationPRO_0000398215
Transit peptidei1 – ?Mitochondrion Reviewed prediction

Interactioni

Protein-protein interaction databases

STRINGi7176.CPIJ004250-PA.

Structurei

3D structure databases

ProteinModelPortaliB0WAU6.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0320.
HOGENOMiHOG000235998.
KOiK03644.
OMAiCKEGMIT.
OrthoDBiEOG7P2XS7.
PhylomeDBiB0WAU6.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00206. Lipoyl_synth.
InterProiIPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view]
PANTHERiPTHR10949. PTHR10949. 1 hit.
PfamiPF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF005963. Lipoyl_synth. 1 hit.
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00510. lipA. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

B0WAU6-1 [UniParc]FASTAAdd to Basket

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MKYFFSLPFP KVHTTCKHTA AATASQPLQK IRERLESGPG FQEFVQNPSY    50
NREDWSAYEG KLKREKGEQD RLRLPPWLKT KIPMGKNFSR IKDQLRELKL 100
ATVCEEAKCP NIGECWGGGE HGTQTATIML MGDTCTRGCR FCSVKTARAP 150
PPLDPDEPKK TASAIASWGL DYIVLTSVDR DDLPDGGSNH IAETIREIKR 200
QNPRIFVECL APDFRGNLDC IRTVATSGLD VYAHNIETVE SLTPFVRDRR 250
AEYRQSLKCL ASVKEINPNM VTKTSIMLGL GETDEQVEQT MKDLRAVGVD 300
CLTLGQYMQP TKRHLKVIEY VTPEKFKHWE TRGNELGFLY TASGPLVRSS 350
YKAGEFFITS ILKNRAAAAA EEATATKPSE 380
Length:380
Mass (Da):42,657
Last modified:April 8, 2008 - v1
Checksum:i0DAB0709245B4EF8
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
DS231875 Genomic DNA. Translation: EDS41812.1.
RefSeqiXP_001845830.1. XM_001845778.1.

Genome annotation databases

EnsemblMetazoaiCPIJ004250-RA; CPIJ004250-PA; CPIJ004250.
GeneIDi6035696.
KEGGicqu:CpipJ_CPIJ004250.
VectorBaseiCPIJ004250. Culex quinquefasciatus.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
DS231875 Genomic DNA. Translation: EDS41812.1 .
RefSeqi XP_001845830.1. XM_001845778.1.

3D structure databases

ProteinModelPortali B0WAU6.
ModBasei Search...

Protein-protein interaction databases

STRINGi 7176.CPIJ004250-PA.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblMetazoai CPIJ004250-RA ; CPIJ004250-PA ; CPIJ004250 .
GeneIDi 6035696.
KEGGi cqu:CpipJ_CPIJ004250.
VectorBasei CPIJ004250. Culex quinquefasciatus.

Phylogenomic databases

eggNOGi COG0320.
HOGENOMi HOG000235998.
KOi K03644.
OMAi CKEGMIT.
OrthoDBi EOG7P2XS7.
PhylomeDBi B0WAU6.

Enzyme and pathway databases

UniPathwayi UPA00538 ; UER00593 .

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_00206. Lipoyl_synth.
InterProi IPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view ]
PANTHERi PTHR10949. PTHR10949. 1 hit.
Pfami PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF005963. Lipoyl_synth. 1 hit.
SMARTi SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00510. lipA. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: JHB.

Entry informationi

Entry nameiLIAS_CULQU
AccessioniPrimary (citable) accession number: B0WAU6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 5, 2010
Last sequence update: April 8, 2008
Last modified: June 11, 2014
This is version 39 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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