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B0WAU6

- LIAS_CULQU

UniProt

B0WAU6 - LIAS_CULQU

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Protein

Lipoyl synthase, mitochondrial

Gene

CPIJ004250

Organism
Culex quinquefasciatus (Southern house mosquito) (Culex pungens)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives.UniRule annotation

Catalytic activityi

Protein N(6)-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 2 [4Fe-4S] clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi104 – 1041Iron-sulfur 1 (4Fe-4S)UniRule annotation
Metal bindingi109 – 1091Iron-sulfur 1 (4Fe-4S)UniRule annotation
Metal bindingi115 – 1151Iron-sulfur 1 (4Fe-4S)UniRule annotation
Metal bindingi135 – 1351Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation
Metal bindingi139 – 1391Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation
Metal bindingi142 – 1421Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
  2. lipoate synthase activity Source: UniProtKB-HAMAP
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. protein lipoylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00538; UER00593.

Names & Taxonomyi

Protein namesi
Recommended name:
Lipoyl synthase, mitochondrialUniRule annotation (EC:2.8.1.8UniRule annotation)
Alternative name(s):
Lipoate synthaseUniRule annotation
Short name:
LSUniRule annotation
Short name:
Lip-synUniRule annotation
Lipoic acid synthaseUniRule annotation
Gene namesi
ORF Names:CPIJ004250
OrganismiCulex quinquefasciatus (Southern house mosquito) (Culex pungens)
Taxonomic identifieri7176 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraNematoceraCulicoideaCulicidaeCulicinaeCuliciniCulexCulex
ProteomesiUP000002320: Partially assembled WGS sequence

Subcellular locationi

Mitochondrion UniRule annotation

GO - Cellular componenti

  1. mitochondrion Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini? – 380Lipoyl synthase, mitochondrialPRO_0000398215
Transit peptidei1 – ?MitochondrionUniRule annotation

Interactioni

Protein-protein interaction databases

STRINGi7176.CPIJ004250-PA.

Structurei

3D structure databases

ProteinModelPortaliB0WAU6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the radical SAM superfamily. Lipoyl synthase family.UniRule annotation

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0320.
HOGENOMiHOG000235998.
InParanoidiB0WAU6.
KOiK03644.
OMAiCKEGMIT.
OrthoDBiEOG7P2XS7.
PhylomeDBiB0WAU6.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00206. Lipoyl_synth.
InterProiIPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view]
PANTHERiPTHR10949. PTHR10949. 1 hit.
PfamiPF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF005963. Lipoyl_synth. 1 hit.
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00510. lipA. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

B0WAU6-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKYFFSLPFP KVHTTCKHTA AATASQPLQK IRERLESGPG FQEFVQNPSY
60 70 80 90 100
NREDWSAYEG KLKREKGEQD RLRLPPWLKT KIPMGKNFSR IKDQLRELKL
110 120 130 140 150
ATVCEEAKCP NIGECWGGGE HGTQTATIML MGDTCTRGCR FCSVKTARAP
160 170 180 190 200
PPLDPDEPKK TASAIASWGL DYIVLTSVDR DDLPDGGSNH IAETIREIKR
210 220 230 240 250
QNPRIFVECL APDFRGNLDC IRTVATSGLD VYAHNIETVE SLTPFVRDRR
260 270 280 290 300
AEYRQSLKCL ASVKEINPNM VTKTSIMLGL GETDEQVEQT MKDLRAVGVD
310 320 330 340 350
CLTLGQYMQP TKRHLKVIEY VTPEKFKHWE TRGNELGFLY TASGPLVRSS
360 370 380
YKAGEFFITS ILKNRAAAAA EEATATKPSE
Length:380
Mass (Da):42,657
Last modified:April 8, 2008 - v1
Checksum:i0DAB0709245B4EF8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DS231875 Genomic DNA. Translation: EDS41812.1.
RefSeqiXP_001845830.1. XM_001845778.1.

Genome annotation databases

EnsemblMetazoaiCPIJ004250-RA; CPIJ004250-PA; CPIJ004250.
GeneIDi6035696.
KEGGicqu:CpipJ_CPIJ004250.
VectorBaseiCPIJ004250. Culex quinquefasciatus.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DS231875 Genomic DNA. Translation: EDS41812.1 .
RefSeqi XP_001845830.1. XM_001845778.1.

3D structure databases

ProteinModelPortali B0WAU6.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 7176.CPIJ004250-PA.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblMetazoai CPIJ004250-RA ; CPIJ004250-PA ; CPIJ004250 .
GeneIDi 6035696.
KEGGi cqu:CpipJ_CPIJ004250.
VectorBasei CPIJ004250. Culex quinquefasciatus.

Phylogenomic databases

eggNOGi COG0320.
HOGENOMi HOG000235998.
InParanoidi B0WAU6.
KOi K03644.
OMAi CKEGMIT.
OrthoDBi EOG7P2XS7.
PhylomeDBi B0WAU6.

Enzyme and pathway databases

UniPathwayi UPA00538 ; UER00593 .

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_00206. Lipoyl_synth.
InterProi IPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view ]
PANTHERi PTHR10949. PTHR10949. 1 hit.
Pfami PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF005963. Lipoyl_synth. 1 hit.
SMARTi SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00510. lipA. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: JHB.

Entry informationi

Entry nameiLIAS_CULQU
AccessioniPrimary (citable) accession number: B0WAU6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 5, 2010
Last sequence update: April 8, 2008
Last modified: November 26, 2014
This is version 42 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3