B0W429 (FICD_CULQU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Adenosine monophosphate-protein transferase FICD homolog
EC=2.7.7.n1

Gene names

ORF Names:

CPIJ001789

Organism

Culex quinquefasciatus (Southern house mosquito) (Culex pungens) [Complete proteome]

Taxonomic identifier

7176 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Panarthropoda › Arthropoda › Mandibulata › Pancrustacea › Hexapoda › Insecta › Dicondylia › Pterygota › Neoptera › Endopterygota › Diptera › Nematocera › Culicimorpha › Culicoidea › Culicidae › Culicinae › Culicini › Culex › Culex › Culex pipiens complex

Protein attributes

Sequence length	500 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Adenylyltransferase that mediates the addition of adenosine 5'-monophosphate (AMP) to specific residues of target proteins By similarity.
Catalytic activity	ATP + [protein] = diphosphate + [protein]-AMP.
Enzyme regulation	Adenylyltransferase activity is inhibited by the inhibitory helix present at the N-terminus: Glu-251 binds ATP and competes with ATP-binding at Arg-391, thereby preventing adenylyltransferase activity. Activation dissociates ATP-binding from Glu-251, allowing ordered binding of the entire ATP moiety with the alpha-phosphate in an orientation that is productive for accepting an incoming target hydroxyl side chain By similarity.
Subcellular location	Membrane; Single-pass membrane protein Potential.
Domain	The fido domain mediates the adenylyltransferase activity By similarity.
Sequence similarities	Belongs to the fic family. Contains 1 fido domain. Contains 2 TPR repeats.

Ontologies

Keywords
Cellular component	Membrane
Domain	Repeat TPR repeat Transmembrane Transmembrane helix
Ligand	ATP-binding Nucleotide-binding
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein adenylyltransferase activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 500	500	Adenosine monophosphate-protein transferase FICD homolog		PRO_0000381781
Regions
	Transmembrane	39 – 59	21	Helical; Potential
	Repeat	122 – 155	34	TPR 1
	Repeat	156 – 190	35	TPR 2
	Domain	302 – 437	136	Fido
	Nucleotide binding	277 – 278	2	ATP By similarity
	Nucleotide binding	385 – 387	3	ATP By similarity
	Motif	247 – 252	6	Inhibitory (S/T)XXXE(G/N) motif
Sites
	Binding site	251	1	ATP By similarity
	Binding site	391	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	B0W429 [UniParc]. Last modified April 8, 2008. Version 1. Checksum: 0BEC135F0CB5B1CA	FASTA	500	56,031
	10 20 30 40 50 60 MCSVCEGSAE GLVTRKRTHS GGGRSAGSKR KWHRRMGGLS FLIFFVIGSL FSGLMFALLS 70 80 90 100 110 120 YAPSYRSRVT PHYLPDGRFL QIADEARVLE PYLSAAVRLP DSLNGTPAGK QQQTARSNEQ 130 140 150 160 170 180 EALSSLKVAV EMKLMGKDDK ALRLFQHAMA LSPRHPEILT KYGEFLEHSQ QDIVTADHYY 190 200 210 220 230 240 YQALTVNPSH SEALANRQRT ASIVEHLDQK RFERLDKKRD ALSSVHALDA GLKRAEKEAY 250 260 270 280 290 300 IQHIYHSVGI EGNTMSLAQT RSILETKMAV DGKSIDEHNE ILGLDAAMKY INATLVNKND 310 320 330 340 350 360 FITLKDLLEI HRRVLGHVDP VEGGEFRRTQ VYVGGHIPPG PGDLSILMSR FEGWLNAEQS 370 380 390 400 410 420 FLMHPVRYAA MAHYKLVHIH PFSDGNGRTS RLLMNTLLMR AGYPPVIIQK QHRHKYYDYL 430 440 450 460 470 480 QVANEGDIRP FVRFIADCTE RTLDLYLWAT SELSHPVPLL AQEEMGGAIG EREHGFGREG 490 500 GSTVHEGSGT GDSIRIGTMW

References

[1]

"Annotation of Culex pipiens quinquefasciatus." The Broad Institute Genome Sequencing Platform Atkinson P.W., Hemingway J., Christensen B.M., Higgs S., Kodira C.D., Hannick L.I., Megy K., O'Leary S.B., Pearson M., Haas B.J., Mauceli E., Wortman J.R., Lee N.H., Guigo R., Stanke M., Alvarado L., Amedeo P., Antoine C.H. , Arensburger P., Bidwell S.L., Crawford M., Camaro F., Devon K., Engels R., Hammond M., Howarth C., Koehrsen M., Lawson D., Montgomery P., Nene V., Nusbaum C., Puiu D., Romero-Severson J., Severson D.W., Shumway M., Sisk P., Stolte C., Zeng Q., Eisenstadt E., Fraser-Liggett C.M., Strausberg R., Galagan J., Birren B., Collins F.H. Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: JHB.

Cross-references

Sequence databases
EMBL GenBank DDBJ	DS231834 Genomic DNA. Translation: EDS32622.1.
RefSeq	XP_001843463.1. XM_001843411.1.
3D structure databases
ProteinModelPortal	B0W429.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	CPIJ001789-RA; CPIJ001789-PA; CPIJ001789.
GeneID	6032944.
KEGG	cqu:CpipJ_CPIJ001789.
VectorBase	CPIJ001789. Culex quinquefasciatus.
Phylogenomic databases
eggNOG	COG3177.
HOGENOM	HOG000008059.
OMA	MKYINTT.
OrthoDB	EOG4XSJ5D.
PhylomeDB	B0W429.
Family and domain databases
Gene3D	1.25.40.10. 1 hit.
InterPro	IPR003812. Fido. IPR013026. TPR-contain_dom. IPR011990. TPR-like_helical. [Graphical view]
Pfam	PF02661. Fic. 1 hit. [Graphical view]
SUPFAM	SSF140931. SSF140931. 1 hit.
PROSITE	PS51459. FIDO. 1 hit. PS50005. TPR. False negative. PS50293. TPR_REGION. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FICD_CULQU

Accession

Primary (citable) accession number: B0W429

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 1, 2009
Last sequence update:	April 8, 2008
Last modified:	March 6, 2013
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)