B0VRC7 (SYD_ACIBS) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Aspartate--tRNA ligase
EC=6.1.1.12

Alternative name(s):
Aspartyl-tRNA synthetase
Short name=AspRS

Gene names

Name:	aspS
Ordered Locus Names:	ABSDF0538

Organism

Acinetobacter baumannii (strain SDF) [Complete proteome] [HAMAP]

Taxonomic identifier

509170 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Moraxellaceae › Acinetobacter › Acinetobacter calcoaceticus/baumannii complex

Protein attributes

Sequence length	592 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-aspartate + tRNA(Asp) = AMP + diphosphate + L-aspartyl-tRNA(Asp). HAMAP MF_00044_B
Subunit structure	Homodimer By similarity. HAMAP MF_00044_B
Subcellular location	Cytoplasm By similarity HAMAP MF_00044_B.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tRNA aminoacylation for protein translation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW aspartate-tRNA ligase activity Inferred from electronic annotation. Source: EC nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 592	592	Aspartate--tRNA ligase HAMAP MF_00044_B		PRO_1000090948

Sequences

	Sequence		Length	Mass (Da)
	B0VRC7 [UniParc]. Last modified April 8, 2008. Version 1. Checksum: F53C46D58B017C0A	FASTA	592	66,623
	10 20 30 40 50 60 MMRTHYCGSL TEAQIDQTVT LCGWVHRRRD HGGVIFLDMR DRDGLVQVVI DPDTPEAFAT 70 80 90 100 110 120 ADKARSEYVL KITGRVRRRY EGTENPNMVS GQIEVLGKEI EVLAASETPP FPLNDDTINV 130 140 150 160 170 180 SEEHRLKYRF LDIRRPEMLD RLRFRSKVTN LIRNYLDDHG FLDVETPILT RATPEGARDY 190 200 210 220 230 240 LVPSRVQNGS FYALPQSPQL FKQLLMVGGI DRYYQIAKCF RDEDLRADRQ PEFTQIDIET 250 260 270 280 290 300 SFLNDDDIMD LMEGMTVKLF NDLLGVKFEK FRRMPYSEAM RDYASDKPDL RIPLKLVDVA 310 320 330 340 350 360 DLMQEVEFKV FAGPAKDPKG RIAALRVPGA GSLTRSQIDE YTKFVGIYGA KGLAYIKVNE 370 380 390 400 410 420 IEKGIEGLQS PIVKFIEPIV MQLLERVGAE NGDIVFFGAD KAKIVNDAMG ALRVKIGHDL 430 440 450 460 470 480 NLATCEWAPL WVVDFPMFEE ADDGKWTSVH HPFTLPKSSV EDVKSNPGEA LSVAYDMVLN 490 500 510 520 530 540 GTEVGGGSLR IYTLEMQKAI FEALGISDEE AEEKFSFLLN ALRYGAPPHG GLAFGLDRLV 550 560 570 580 590 MLMTGATSIR DVIAFPKTKT AECPLTQAPA PVEANQLRDL GIRLREQQKK EA

References

[1]

"Comparative analysis of Acinetobacters: three genomes for three lifestyles." Vallenet D., Nordmann P., Barbe V., Poirel L., Mangenot S., Bataille E., Dossat C., Gas S., Kreimeyer A., Lenoble P., Oztas S., Poulain J., Segurens B., Robert C., Abergel C., Claverie J.-M., Raoult D., Medigue C., Weissenbach J., Cruveiller S. PLoS ONE 3:E1805-E1805(2008) [PubMed: 18350144] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SDF.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CU468230 Genomic DNA. Translation: CAO99921.1.
RefSeq	YP_001706179.1. NC_010400.1.
3D structure databases
ProteinModelPortal	B0VRC7.
SMR	B0VRC7. Positions 2-585.
ModBase	Search...
Protein-protein interaction databases
STRING	B0VRC7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5984241.
GenomeReviews	Gene locus ABSDF0538 in contig CU468230_GR.
KEGG	abm:ABSDF0538.
PATRIC	20732034. VBIAciBau88365_0549.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG396032.
OMA	AFPKTQQ.
ProtClustDB	PRK00476.
Family and domain databases
HAMAP	MF_00044_B. Asp_tRNA_synth_B. [Tree]
InterPro	IPR004364. aa-tRNA-synt_II. IPR018150. aa-tRNA-synt_II-like. IPR006195. aa-tRNA-synth_II. IPR004524. Asp-tRNA-synth_IIb_bac/mt. IPR002312. Asp/Asn-tRNA-synth_IIb. IPR004115. GAD_dom. IPR012340. NA-bd_OB-fold. IPR016027. NA-bd_OB-fold-like. IPR004365. NA-bd_OB_tRNA-helicase. [Graphical view]
Gene3D	G3DSA:3.30.1360.30. GAD_dom. 1 hit. G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
KO	K01876.
PANTHER	PTHR22594. aa-tRNA-synt_II. 1 hit. PTHR22594:SF5. AspS_bac. 1 hit.
Pfam	PF02938. GAD. 1 hit. PF00152. tRNA-synt_2. 1 hit. PF01336. tRNA_anti. 1 hit. [Graphical view]
PRINTS	PR01042. TRNASYNTHASP.
SUPFAM	SSF50249. Nucleic_acid_OB. 1 hit. SSF55261. SSF55261. 1 hit.
TIGRFAMs	TIGR00459. AspS_bact. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYD_ACIBS

Accession

Primary (citable) accession number: B0VRC7

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	April 8, 2008
Last modified:	January 25, 2012
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program