Reviewed, UniProtKB/Swiss-Prot B0VKT1 (PROA_ACIBS)

Names and origin

Protein names

Recommended name:
    Gamma-glutamyl phosphate reductase
      Short name=GPR
    EC=1.2.1.41
Alternative name(s):
    Glutamate-5-semialdehyde dehydrogenase
    Glutamyl-gamma-semialdehyde dehydrogenase
      Short name=GSA dehydrogenase

Gene names

Name:	proA
Ordered Locus Names:	ABSDF3014

Organism

Acinetobacter baumannii (strain SDF) [Complete proteome] [HAMAP]

Taxonomic identifier

509170 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Moraxellaceae › Acinetobacter

Protein attributes

Sequence length	421 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the NADPH dependent reduction of L-gamma-glutamyl 5-phosphate into L-glutamate 5-semialdehyde and phosphate. The product spontaneously undergoes cyclization to form 1-pyrroline-5-carboxylate By similarity. HAMAP MF_00412
Catalytic activity	L-glutamate 5-semialdehyde + phosphate + NADP+ = L-glutamyl 5-phosphate + NADPH. HAMAP MF_00412
Pathway	Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 2/2. HAMAP MF_00412
Subcellular location	Cytoplasm By similarity HAMAP MF_00412.
Sequence similarities	Belongs to the gamma-glutamyl phosphate reductase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Proline biosynthesis
Cellular component	Cytoplasm
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW proline biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NADP or NADPH binding Inferred from electronic annotation. Source: InterPro glutamate-5-semialdehyde dehydrogenase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 421	421	Gamma-glutamyl phosphate reductase HAMAP MF_00412		PRO_1000123768

Sequences

	Sequence		Length	Mass (Da)
	B0VKT1-1 [UniParc]. Last modified April 8, 2008. Version 1. Checksum: CC88F9E755654E46	FASTA	421	45,696
	10 20 30 40 50 60 MQDSIEQYMQ KVGQQARDAS RVLTSASTSL KNHALSAIYT ALENNQAAIL AANQIDMEKG 70 80 90 100 110 120 RSNQLDSALL DRLELTPARF KGMLQGLKDV IALVDPIGEI TDLAYRPTGI QIGKMRVPLG 130 140 150 160 170 180 VVGMIYESRP NVTLEAASLA IKSGNAIILR GGSEALESNK AIAEAVKHGL KVAGLPEHSV 190 200 210 220 230 240 QVIETSDRAA VGHLITMAEY VDVIVPRGGK SLIERVTNEA RIPVIKHLDG NCHVFVEAQA 250 260 270 280 290 300 DLQKALPITL NAKTHRYGVC NAMETLLVDE KIAEVFLPHI AELYAEKQVE LRGCPETHRI 310 320 330 340 350 360 LGTTVKPATE EDWYTEYLGP ILAVKVVSGI DEAIDHINKY GSHHTDAIVT ENYTLARQFL 370 380 390 400 410 420 ARVDSSSVVV NASTRFADGF EYGLGAEIGI STDKIHARGP VGLEGLTSQK WIVLGDGQIR Q

References

[1]

"Comparative analysis of Acinetobacters: three genomes for three lifestyles." Vallenet D., Nordmann P., Barbe V., Poirel L., Mangenot S., Bataille E., Dossat C., Gas S., Kreimeyer A., Lenoble P., Oztas S., Poulain J., Segurens B., Robert C., Abergel C., Claverie J.-M., Raoult D., Medigue C., Weissenbach J., Cruveiller S. PLoS ONE 3:E1805-E1805(2008) [PubMed: 18350144] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CU468230 Genomic DNA. Translation: CAP02300.1.
RefSeq	YP_001708152.1.
3D structure databases
SMR	B0VKT1. Positions 14-421.
ModBase	Search...
Genome annotation databases
GeneID	5986001.
GenomeReviews	Gene locus ABSDF3014 in contig CU468230_GR.
KEGG	abm:ABSDF3014.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG318080.
OMA	QYPAACN.
Family and domain databases
HAMAP	MF_00412. ProA. [Tree]
InterPro	IPR016161. Ald_DH/histidinol_DH. IPR016163. Ald_DH_C. IPR016162. Ald_DH_N. IPR015590. Aldehyde_DH. IPR000965. G-glutamylP_reductase. IPR020593. G-glutamylP_reductase_CS. IPR012134. Glu-5-SA_DH. [Graphical view]
Gene3D	G3DSA:3.40.309.10. Aldehyde_dehydrogenase_C. 1 hit. G3DSA:3.40.605.10. Aldehyde_dehydrogenase_N. 1 hit.
PANTHER	PTHR11063:SF1. GSA_DH. 1 hit.
Pfam	PF00171. Aldedh. 2 hits. [Graphical view]
PIRSF	PIRSF000151. GPR. 1 hit.
TIGRFAMs	TIGR00407. proA. 1 hit.
PROSITE	PS01223. PROA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PROA_ACIBS

Accession

Primary (citable) accession number: B0VKT1

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	April 8, 2008
Last modified:	February 9, 2010
This is version 23 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)