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B0VKP9 (SYL_ACIBS) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 47. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:ABSDF2980
OrganismAcinetobacter baumannii (strain SDF) [Complete proteome] [HAMAP]
Taxonomic identifier509170 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesMoraxellaceaeAcinetobacterAcinetobacter calcoaceticus/baumannii complex

Protein attributes

Sequence length874 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 874874Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_1000091280

Regions

Motif47 – 5711"HIGH" region HAMAP-Rule MF_00049
Motif636 – 6405"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site6391ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
B0VKP9 [UniParc].

Last modified April 8, 2008. Version 1.
Checksum: 6AEEF802D3C70895

FASTA87498,024
        10         20         30         40         50         60 
MTISHIDPEY QANTIEPSVQ QDWENRKVFK VADTVEGKHR YILSMFPYPS GKLHMGHVRN 

        70         80         90        100        110        120 
YTIGDVISRF YRLKGETVLQ PMGWDAFGLP AENAAIAHKV APAKWTFENI AYMRDQLKKL 

       130        140        150        160        170        180 
GLSVDWDREF ATCTPEYYHW EQWLFVQLYK KGLIYRKLST VNWDPVDQTV LANEQVENGR 

       190        200        210        220        230        240 
GWRSGALVEK RDIPMYYFRI TDYAQELLDD LDTLQDGWPQ QVLTMQRNWI GRSTGMEITF 

       250        260        270        280        290        300 
PSANTEIYAD GLTVYTTRAD TLMGVTYVAV AAEHPLALKA AENNPELAAF IEECRIGSVA 

       310        320        330        340        350        360 
EADLATAEKK GMATGLFVKH PVTGEELPVW IANYVLMSYG SGAVMAVPAH DERDFEFANK 

       370        380        390        400        410        420 
FNLPIKQVID AKGADDADYS ATEWQEWYGS KEGKLVNSGE FDGLEFQAAF DAFLAKLEPQ 

       430        440        450        460        470        480 
GLANSKVQFR LRDWGVSRQR YWGCPIPMIN CDTCGQVTVP EDQLPVVLPT DVVPDGSGNP 

       490        500        510        520        530        540 
LNKMPEFYET KCPCCGGDAR RETDTLDTFV ESSWYYARYA SPDFTGGIVK PEAAKNWLPV 

       550        560        570        580        590        600 
NQYIGGVEHA ILHLLYARFF HKLMHDEGVV QGNEPFTNLL TQGMVLADTF YREAENGKKT 

       610        620        630        640        650        660 
WFNPANIELE RDEKGRIISA KYSGDGQEVI IGGQEKMSKS KNNGIDPQAI IDQYGADTAR 

       670        680        690        700        710        720 
VFMMFAAPPD QSLEWSDAGV EGANRFLKRV WRLVASFLEK GNSATAIDKA NLSKDAQDLR 

       730        740        750        760        770        780 
RKTHETIQKV SDDIERRHAF NTAIAALMEL LNASNKFEAK DDNDVAVERE AITTLLTLLA 

       790        800        810        820        830        840 
PFAPHLSQTL LAQFGTDLTE ATFPEVDASA LTRNTQTIVV QVNGKLRGKL EVSVDISKDE 

       850        860        870 
LLAQAKALPE VQQFLTGPTK KEIVVPNKLV NLVV 

« Hide

References

[1]"Comparative analysis of Acinetobacters: three genomes for three lifestyles."
Vallenet D., Nordmann P., Barbe V., Poirel L., Mangenot S., Bataille E., Dossat C., Gas S., Kreimeyer A., Lenoble P., Oztas S., Poulain J., Segurens B., Robert C., Abergel C., Claverie J.-M., Raoult D., Medigue C., Weissenbach J., Cruveiller S.
PLoS ONE 3:E1805-E1805(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: SDF.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CU468230 Genomic DNA. Translation: CAP02268.1.
RefSeqYP_001708122.1. NC_010400.1.

3D structure databases

ProteinModelPortalB0VKP9.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING509170.ABSDF2980.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAP02268; CAP02268; ABSDF2980.
GeneID5985982.
KEGGabm:ABSDF2980.
PATRIC20736667. VBIAciBau88365_2820.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200747.
KOK01869.
OMAHISHELW.
OrthoDBEOG63Z74X.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_ACIBS
AccessionPrimary (citable) accession number: B0VKP9
Entry history
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: April 8, 2008
Last modified: May 14, 2014
This is version 47 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries