Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

B0VDR1 (PCKG_ACIBY) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 39. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Phosphoenolpyruvate carboxykinase [GTP]

Short name=PEP carboxykinase
Short name=PEPCK
EC=4.1.1.32
Gene names
Name:pckG
Ordered Locus Names:ABAYE0818
OrganismAcinetobacter baumannii (strain AYE) [Complete proteome] [HAMAP]
Taxonomic identifier509173 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesMoraxellaceaeAcinetobacterAcinetobacter calcoaceticus/baumannii complex

Protein attributes

Sequence length610 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle By similarity. HAMAP-Rule MF_00452

Catalytic activity

GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO2. HAMAP-Rule MF_00452

Cofactor

Binds 1 manganese ion per subunit By similarity. HAMAP-Rule MF_00452

Pathway

Carbohydrate biosynthesis; gluconeogenesis. HAMAP-Rule MF_00452

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00452

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00452.

Sequence similarities

Belongs to the phosphoenolpyruvate carboxykinase [GTP] family.

Ontologies

Keywords
   Biological processGluconeogenesis
   Cellular componentCytoplasm
   LigandGTP-binding
Manganese
Metal-binding
Nucleotide-binding
   Molecular functionDecarboxylase
Lyase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processgluconeogenesis

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionGTP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

manganese ion binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

phosphoenolpyruvate carboxykinase (GTP) activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 610610Phosphoenolpyruvate carboxykinase [GTP] HAMAP-Rule MF_00452
PRO_1000125042

Regions

Nucleotide binding268 – 2736GTP By similarity
Nucleotide binding516 – 5194GTP By similarity
Region382 – 3843Substrate binding By similarity

Sites

Active site2691 By similarity
Metal binding2251Manganese By similarity
Metal binding2451Manganese; via tele nitrogen By similarity
Metal binding2941Manganese By similarity
Binding site771Substrate By similarity
Binding site2181Substrate; via amide nitrogen By similarity
Binding site2251Substrate By similarity
Binding site2671Substrate By similarity
Binding site3841GTP By similarity
Binding site4151GTP By similarity

Sequences

Sequence LengthMass (Da)Tools
B0VDR1 [UniParc].

Last modified April 8, 2008. Version 1.
Checksum: 6FAD9D3231AD796E

FASTA61067,448
        10         20         30         40         50         60 
MTTVNAPEFV RHPKLIAWVE EIANLTKPAK IEWCDGSEEE YQRLIDLMIA NGTMQKLNQE 

        70         80         90        100        110        120 
KHPGSYLANS DPSDVARVED RTYICSQNKE DAGATNNWED PAVMREKLNG LFEGSMKGRT 

       130        140        150        160        170        180 
MYVVPFSMGP LGSHIAHIGI ELTDSPYVAV SMRKMARMGK AVYDVLGTDG EFVPCVHTVG 

       190        200        210        220        230        240 
APLAEGQKDV AWPCNPEKYI VHYPETREIW SFGSGYGGNA LLGKKCLALR IASVMGREQG 

       250        260        270        280        290        300 
WLAEHMLILG VTNPQGEKHY IAAAFPSACG KTNFAMLIPP AGYEGWKIET VGDDIAWIKP 

       310        320        330        340        350        360 
GEDGRLYAIN PEAGFFGVAP GTNTKTNPNC MATLHKDVIY TNVAVTDDGQ VWWEGLSKEV 

       370        380        390        400        410        420 
PANLTNWKGQ PHVNGEKAAH PNARFTVAAG QCPSIDADWE NPAGVPISAF IFGGRRADTV 

       430        440        450        460        470        480 
PLVSEAFDWV DGVYKAATMG SETTAAAVGQ QGIVRRDPFA MLPFAGYNMA DYFDHWLNLG 

       490        500        510        520        530        540 
AKVSEKAEAS GNKLPKIFNV NWFRRDAEGN FVWPGFGQNM RVLEWIIDRC EGRANAVETP 

       550        560        570        580        590        600 
IGFVPTYEDL NWEGTEFTKE QFDLITNQDR DQWVTEIESH TELFNKLGER LPKALKERQA 

       610 
ALLEAVKTGF 

« Hide

References

[1]"Comparative analysis of Acinetobacters: three genomes for three lifestyles."
Vallenet D., Nordmann P., Barbe V., Poirel L., Mangenot S., Bataille E., Dossat C., Gas S., Kreimeyer A., Lenoble P., Oztas S., Poulain J., Segurens B., Robert C., Abergel C., Claverie J.-M., Raoult D., Medigue C., Weissenbach J., Cruveiller S.
PLoS ONE 3:E1805-E1805(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: AYE.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CU459141 Genomic DNA. Translation: CAM85771.1.
RefSeqYP_001712774.1. NC_010410.1.

3D structure databases

ProteinModelPortalB0VDR1.
SMRB0VDR1. Positions 10-604.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING509173.ABAYE0818.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAM85771; CAM85771; ABAYE0818.
GeneID6003277.
KEGGaby:ABAYE0818.
PATRIC20725101. VBIAciBau69881_0879.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1274.
HOGENOMHOG000191700.
KOK01596.
OMADWHGKTW.
OrthoDBEOG6MPWQS.

Enzyme and pathway databases

BioCycABAU509173:GJXF-819-MONOMER.
UniPathwayUPA00138.

Family and domain databases

Gene3D3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPMF_00452. PEPCK_GTP.
InterProIPR018091. PEP_carboxykin_GTP_CS.
IPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view]
PANTHERPTHR11561. PTHR11561. 1 hit.
PfamPF00821. PEPCK. 1 hit.
[Graphical view]
PIRSFPIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMSSF68923. SSF68923. 1 hit.
PROSITEPS00505. PEPCK_GTP. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePCKG_ACIBY
AccessionPrimary (citable) accession number: B0VDR1
Entry history
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: April 8, 2008
Last modified: May 14, 2014
This is version 39 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways