Reviewed, UniProtKB/Swiss-Prot B0VAU1 (SYC_ACIBY)

Names and origin

Protein names

Recommended name:
    Cysteinyl-tRNA synthetase
    EC=6.1.1.16
Alternative name(s):
    Cysteine--tRNA ligase
      Short name=CysRS

Gene names

Name:	cysS
Ordered Locus Names:	ABAYE2493

Organism

Acinetobacter baumannii (strain AYE) [Complete proteome] [HAMAP]

Taxonomic identifier

509173 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Moraxellaceae › Acinetobacter

Protein attributes

Sequence length	473 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-cysteine + tRNA(Cys) = AMP + diphosphate + L-cysteinyl-tRNA(Cys). HAMAP MF_00041
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00041
Subunit structure	Monomer By similarity. HAMAP MF_00041
Subcellular location	Cytoplasm By similarity HAMAP MF_00041.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cysteinyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP cysteine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 473	473	Cysteinyl-tRNA synthetase HAMAP MF_00041		PRO_1000090811
Regions
	Motif	32 – 42	11	"HIGH" region HAMAP MF_00041
	Motif	270 – 274	5	"KMSKS" region HAMAP MF_00041
Sites
	Metal binding	30	1	Zinc By similarity
	Metal binding	213	1	Zinc By similarity
	Metal binding	238	1	Zinc By similarity
	Metal binding	242	1	Zinc By similarity
	Binding site	273	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	B0VAU1-1 [UniParc]. Last modified April 8, 2008. Version 1. Checksum: 744B8D17B0E483FB	FASTA	473	54,170
	10 20 30 40 50 60 MQPFVLYNSE QRKKVEFVPR KEGHIDMYVC GMTVYDYCHI GHARVMVAFD YIIRFLRSQG 70 80 90 100 110 120 WKVRYIRNIT DIDDKIIKRA NENGETIQQL TTRFIDAMNE DAANLGCLAP DEAPKATEYI 130 140 150 160 170 180 DQMQNMIGNL VNKGAAYPAS NGDVYFEVTK FEKYGRLSGR KLDDMQAGAS ERIDVEVEKK 190 200 210 220 230 240 HPFDFVLWKH AKENEPSWAS PWGNGRPGWH IECSAMSTCC LGNHFDIHGG GSDLMFPHHE 250 260 270 280 290 300 NEIAQSEAST GEQYVNYWMH VGFINVDGEK MSKSLGNFFT IRDVMEKFHP EVIRYFIVSS 310 320 330 340 350 360 HYRSPVNFSD VALKEAKTSL TRFYHSFKAY QQVYGQTTTE ALDQSFIERF NNAMCDDFNT 370 380 390 400 410 420 AEAMAVLFEL NKELNRAVKE EQADQATVLY STLRHLTNIL GLVQHNVDDF LKSDIGQDAL 430 440 450 460 470 ALSDAEIEDF IQQRVDAKKA KDFAKADSIR QSLLEQGVVL EDTRQGTVWR RAD

References

[1]

"Comparative analysis of Acinetobacters: three genomes for three lifestyles." Vallenet D., Nordmann P., Barbe V., Poirel L., Mangenot S., Bataille E., Dossat C., Gas S., Kreimeyer A., Lenoble P., Oztas S., Poulain J., Segurens B., Robert C., Abergel C., Claverie J.-M., Raoult D., Medigue C., Weissenbach J., Cruveiller S. PLoS ONE 3:E1805-E1805(2008) [PubMed: 18350144] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CU459141 Genomic DNA. Translation: CAM87336.1.
RefSeq	YP_001714316.1.
3D structure databases
SMR	B0VAU1. Positions 5-410.
ModBase	Search...
Genome annotation databases
GeneID	6003414.
GenomeReviews	Gene locus ABAYE2493 in contig CU459141_GR.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG327651.
OMA	VLWKAAK.
Family and domain databases
HAMAP	MF_00041_B. Cys_tRNA_synth_B. [Tree]
InterPro	IPR015804. Cys-tRNA-synt_Ia_C. IPR015273. Cys-tRNA-synt_Ia_DALR. IPR015803. Cys-tRNA-synt_Ia_N. IPR002308. Cys-tRNA-synth_1a. IPR014729. Rossmann-like_a/b/a_fold. IPR009080. tRNAsynth_1a_anticodon-bd. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
PANTHER	PTHR10890. Cys_tRNA-synt_1a. 1 hit.
Pfam	PF09190. DALR_2. 1 hit. PF01406. tRNA-synt_1e. 1 hit. [Graphical view]
PRINTS	PR00983. TRNASYNTHCYS.
SMART	SM00840. DALR_2. 1 hit. [Graphical view]
TIGRFAMs	TIGR00435. cysS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYC_ACIBY

Accession

Primary (citable) accession number: B0VAU1

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	April 8, 2008
Last modified:	February 9, 2010
This is version 17 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)