B0VAE0 (FMT_ACIBY) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Methionyl-tRNA formyltransferase
EC=2.1.2.9

Gene names

Name:	fmt
Ordered Locus Names:	ABAYE0022

Organism

Acinetobacter baumannii (strain AYE) [Complete proteome] [HAMAP]

Taxonomic identifier

509173 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Moraxellaceae › Acinetobacter › Acinetobacter calcoaceticus/baumannii complex › Acinetobacter baumannii

Protein attributes

Sequence length	320 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Modifies the free amino group of the aminoacyl moiety of methionyl-tRNA(fMet). The formyl group appears to play a dual role in the initiator identity of N-formylmethionyl-tRNA by: (I) promoting its recognition by IF2 and (II) impairing its binding to EFTu-GTP By similarity. HAMAP-Rule MF_00182
Catalytic activity	10-formyltetrahydrofolate + L-methionyl-tRNA(fMet) = tetrahydrofolate + N-formylmethionyl-tRNA(fMet). HAMAP-Rule MF_00182
Sequence similarities	Belongs to the fmt family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	translational initiation Inferred from electronic annotation. Source: GOC
Molecular_function	methionyl-tRNA formyltransferase activity Inferred from electronic annotation. Source: HAMAP methyltransferase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 320	320	Methionyl-tRNA formyltransferase HAMAP-Rule MF_00182		PRO_1000098370
Regions
	Region	114 – 117	4	Tetrahydrofolate (THF) binding By similarity

Sequences

	Sequence		Length	Mass (Da)
	B0VAE0 [UniParc]. Last modified April 8, 2008. Version 1. Checksum: 4AC5C6E1E87B0BD8	FASTA	320	34,655
	10 20 30 40 50 60 MKIIFAGTPE FAATALAALL KTSHEIIAVY TQPDRKAGRG QKLTPSPVKQ LALEHNIPVY 70 80 90 100 110 120 QPLHFKASTE EGLAAQQELA ALGADVMVVA AYGLILPQAV LDTPKYGCLN IHGSLLPRWR 130 140 150 160 170 180 GAAPIQRAIA TGDDETGITI MQMAAGLDTG DMMYKTYCPI TSEDTSATLH DKLAAQGATA 190 200 210 220 230 240 ICAVLESEET LQKYLAEREV QDESLTVYAH KLVKSEARID WSMNAVQVDR NIRAFNPWPV 250 260 270 280 290 300 AFIQLDENNA LRVWNSTISS QSKVNAQAGE IIAIDKQGVH VACGENTFIC LTSVQWPGGK 310 320 ALNAQQIAQT QKLHVGQILP

References

[1]

"Comparative analysis of Acinetobacters: three genomes for three lifestyles." Vallenet D., Nordmann P., Barbe V., Poirel L., Mangenot S., Bataille E., Dossat C., Gas S., Kreimeyer A., Lenoble P., Oztas S., Poulain J., Segurens B., Robert C., Abergel C., Claverie J.-M., Raoult D., Medigue C., Weissenbach J., Cruveiller S. PLoS ONE 3:E1805-E1805(2008) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AYE.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CU459141 Genomic DNA. Translation: CAM85012.1.
RefSeq	YP_001712019.1. NC_010410.1.
3D structure databases
ProteinModelPortal	B0VAE0.
SMR	B0VAE0. Positions 1-319.
ModBase	Search...
Protein-protein interaction databases
STRING	509173.ABAYE0022.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAM85012; CAM85012; ABAYE0022.
GeneID	6003931.
KEGG	aby:ABAYE0022.
PATRIC	20723527. VBIAciBau69881_0125.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0223.
HOGENOM	HOG000261177.
KO	K00604.
OMA	GITLMQM.
ProtClustDB	CLSK2307579.
Enzyme and pathway databases
BioCyc	ABAU509173:GJXF-25-MONOMER.
Family and domain databases
Gene3D	3.10.25.10. 1 hit. 3.40.50.170. 1 hit.
HAMAP	MF_00182. Formyl_trans.
InterPro	IPR005794. Fmt. IPR005793. Formyl_trans_C. IPR002376. Formyl_transf_N. IPR011034. Formyl_transferase_C-like. IPR015518. Met_tRNA_Form_TA-like. [Graphical view]
PANTHER	PTHR11138. PTHR11138. 1 hit.
Pfam	PF02911. Formyl_trans_C. 1 hit. PF00551. Formyl_trans_N. 1 hit. [Graphical view]
SUPFAM	SSF50486. FMT_C_like. 1 hit. SSF53328. formyl_transf. 1 hit.
TIGRFAMs	TIGR00460. fmt. 1 hit.
PROSITE	PS00373. GART. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FMT_ACIBY

Accession

Primary (citable) accession number: B0VAE0

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	April 8, 2008
Last modified:	May 1, 2013
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program