B0V782 (THIM_ACIBY) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Hydroxyethylthiazole kinase
EC=2.7.1.50

Alternative name(s):
4-methyl-5-beta-hydroxyethylthiazole kinase
Short name=TH kinase
Short name=Thz kinase

Gene names

Name:	thiM
Ordered Locus Names:	ABAYE1466

Organism

Acinetobacter baumannii (strain AYE) [Complete proteome] [HAMAP]

Taxonomic identifier

509173 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Moraxellaceae › Acinetobacter › Acinetobacter calcoaceticus/baumannii complex › Acinetobacter baumannii

Protein attributes

Sequence length	275 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + 4-methyl-5-(2-hydroxyethyl)thiazole = ADP + 4-methyl-5-(2-phosphonooxyethyl)thiazole. HAMAP-Rule MF_00228
Cofactor	Magnesium By similarity. HAMAP-Rule MF_00228
Pathway	Cofactor biosynthesis; thiamine diphosphate biosynthesis; 4-methyl-5-(2-phosphoethyl)-thiazole from 5-(2-hydroxyethyl)-4-methylthiazole: step 1/1. HAMAP-Rule MF_00228
Sequence similarities	Belongs to the Thz kinase family.

Ontologies

Keywords
Biological process	Thiamine biosynthesis
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	thiamine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW thiamine diphosphate biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP hydroxyethylthiazole kinase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 275	275	Hydroxyethylthiazole kinase HAMAP-Rule MF_00228		PRO_0000383814
Sites
	Binding site	50	1	Substrate; via amide nitrogen By similarity
	Binding site	126	1	ATP By similarity
	Binding site	171	1	ATP By similarity
	Binding site	200	1	Substrate; via amide nitrogen By similarity

Sequences

	Sequence		Length	Mass (Da)
	B0V782 [UniParc]. Last modified April 8, 2008. Version 1. Checksum: 2036927450642BCA	FASTA	275	29,148
	10 20 30 40 50 60 MNSTSNLIEQ VIEAWQNMQA KTPLVQCITN SVAANYTANV LLASGASPAM IDNPYEAESF 70 80 90 100 110 120 TKISSALSIN LGTPTSEQMQ AMQISAKTAQ LNNVPWVLDP VGYGPILAWR SQMTDELLQF 130 140 150 160 170 180 KPSVIRGNAS EISTLAGNQV QSKGVDSTLS SDQAYQQAFS LLTHASCIAI SGESDYILSN 190 200 210 220 230 240 EVDAVIQVNG GSPLQPKITA TGCALGALIA AYSAVTTPTI AALSAHIHFA IAGKLAANQA 250 260 270 QTMGSFSSIF MDYIHMLDAN LIEQYADVKL LNKQA

References

[1]

"Comparative analysis of Acinetobacters: three genomes for three lifestyles." Vallenet D., Nordmann P., Barbe V., Poirel L., Mangenot S., Bataille E., Dossat C., Gas S., Kreimeyer A., Lenoble P., Oztas S., Poulain J., Segurens B., Robert C., Abergel C., Claverie J.-M., Raoult D., Medigue C., Weissenbach J., Cruveiller S. PLoS ONE 3:E1805-E1805(2008) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AYE.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CU459141 Genomic DNA. Translation: CAM86373.1.
RefSeq	YP_001713373.1. NC_010410.1.
3D structure databases
ProteinModelPortal	B0V782.
ModBase	Search...
Protein-protein interaction databases
STRING	509173.ABAYE1466.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAM86373; CAM86373; ABAYE1466.
GeneID	6001231.
KEGG	aby:ABAYE1466.
PATRIC	20726309. VBIAciBau69881_1473.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2145.
HOGENOM	HOG000114352.
KO	K00878.
OMA	SPVMAHA.
ProtClustDB	CLSK707428.
Enzyme and pathway databases
BioCyc	ABAU509173:GJXF-1431-MONOMER.
UniPathway	UPA00060; UER00139.
Family and domain databases
HAMAP	MF_00228. Thz_kinase.
InterPro	IPR000417. Hyethyz_kinase. IPR026012. Hyethyz_kinase/Th_synth. [Graphical view]
PANTHER	PTHR20857:SF14. PTHR20857:SF14. 1 hit.
Pfam	PF02110. HK. 1 hit. [Graphical view]
PIRSF	PIRSF000513. Thz_kinase. 1 hit.
PRINTS	PR01099. HYETHTZKNASE.
ProtoNet	Search...

Entry information

Entry name

THIM_ACIBY

Accession

Primary (citable) accession number: B0V782

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 22, 2009
Last sequence update:	April 8, 2008
Last modified:	May 1, 2013
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program