Reviewed, UniProtKB/Swiss-Prot B0V5Q6 (SYFA_ACIBY)

Names and origin

Protein names

Recommended name:
    Phenylalanyl-tRNA synthetase alpha chain
    EC=6.1.1.20
Alternative name(s):
    Phenylalanine--tRNA ligase alpha chain
      Short name=PheRS

Gene names

Name:	pheS
Ordered Locus Names:	ABAYE3160

Organism

Acinetobacter baumannii (strain AYE) [Complete proteome] [HAMAP]

Taxonomic identifier

509173 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Moraxellaceae › Acinetobacter

Protein attributes

Sequence length	330 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + L-phenylalanyl-tRNA(Phe). HAMAP MF_00281
Cofactor	Binds 2 magnesium ions per tetramer By similarity.
Subunit structure	Tetramer of two alpha and two beta chains By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 1 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	phenylalanyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP phenylalanine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 330	330	Phenylalanyl-tRNA synthetase alpha chain HAMAP MF_00281		PRO_1000114840
Sites
	Metal binding	255	1	Magnesium By similarity

Sequences

	Sequence		Length	Mass (Da)
	B0V5Q6-1 [UniParc]. Last modified April 8, 2008. Version 1. Checksum: D7A94968259FC957	FASTA	330	37,105
	10 20 30 40 50 60 MRVTMSLEAL TTEALAAIAA AQDLVALDQV RVQFTGKKSQ LAEQSKALGK MDPEERKVQG 70 80 90 100 110 120 AAIHAVRETI NNALTERQTA LQQAALAQKL ASETIDITLP GRGQCVGTVH PVTQVQERIC 130 140 150 160 170 180 QFFTKAGFTV ATGPEVEDDY HNFEALNIPG HHPARAMHDT FYFDANHLLR THTSGVQIRT 190 200 210 220 230 240 METSQPPIRI VCPGRVYRCD SDQTHSPMFH QIEGLYVAEN TSFAELKGLL INLLNEFFEK 250 260 270 280 290 300 DLKVRFRPSY FPFTEPSAEV DIMDERGRWL EVLGCGMVHP NVLRAAGIDP DKYKGFAFGL 310 320 330 GVERFAMLRY GINDLRMFYQ NDVRFLRQFA

References

[1]

"Comparative analysis of Acinetobacters: three genomes for three lifestyles." Vallenet D., Nordmann P., Barbe V., Poirel L., Mangenot S., Bataille E., Dossat C., Gas S., Kreimeyer A., Lenoble P., Oztas S., Poulain J., Segurens B., Robert C., Abergel C., Claverie J.-M., Raoult D., Medigue C., Weissenbach J., Cruveiller S. PLoS ONE 3:E1805-E1805(2008) [PubMed: 18350144] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CU459141 Genomic DNA. Translation: CAM87969.1.
RefSeq	YP_001714941.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	6003649.
GenomeReviews	Gene locus ABAYE3160 in contig CU459141_GR.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	FRASYFP.
Family and domain databases
HAMAP	MF_00281. [Tree]
InterPro	IPR006195. aa-tRNA-synth_II_cons-reg. IPR004529. Phe-tRNA-synth_IIc_asu. IPR004188. Phe-tRNA_synth_II_N. IPR002319. Phenylalanyl-tRNA_Synthase_acu. [Graphical view]
PANTHER	PTHR11538. tRNA-synt_2d. 1 hit.
Pfam	PF02912. Phe_tRNA-synt_N. 1 hit. PF01409. tRNA-synt_2d. 1 hit. [Graphical view]
TIGRFAMs	TIGR00468. pheS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYFA_ACIBY

Accession

Primary (citable) accession number: B0V5Q6

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	April 8, 2008
Last modified:	November 3, 2009
This is version 13 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)