B0V4K1 (KATG_ACIBY) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Catalase-peroxidase
Short name=CP
EC=1.11.1.21

Alternative name(s):
Peroxidase/catalase

Gene names

Name:	katG
Ordered Locus Names:	ABAYE3366

Organism

Acinetobacter baumannii (strain AYE) [Complete proteome] [HAMAP]

Taxonomic identifier

509173 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Moraxellaceae › Acinetobacter › Acinetobacter calcoaceticus/baumannii complex

Protein attributes

Sequence length	718 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Bifunctional enzyme with both catalase and broad-spectrum peroxidase activity By similarity. HAMAP MF_01961
Catalytic activity	Donor + H2O2 = oxidized donor + 2 H2O. HAMAP MF_01961 2 H2O2 = O2 + 2 H2O. HAMAP MF_01961
Cofactor	Binds 1 heme B (iron-protoporphyrin IX) group per dimer By similarity.
Subunit structure	Homodimer or homotetramer By similarity. HAMAP MF_01961
Post-translational modification	The covalent Trp-Tyr-Met adduct is important for the catalase, but not the peroxidase activity of the enzyme By similarity. HAMAP MF_01961
Sequence similarities	Belongs to the peroxidase family. Peroxidase/catalase subfamily.

Ontologies

Keywords
Biological process	Hydrogen peroxide
Ligand	Heme Iron Metal-binding
Molecular function	Oxidoreductase Peroxidase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	hydrogen peroxide catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	catalase activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 718	718	Catalase-peroxidase HAMAP MF_01961		PRO_0000354713
Sites
	Active site	99	1	Proton acceptor By similarity
	Metal binding	260	1	Iron (heme axial ligand) By similarity
	Site	95	1	Transition state stabilizer By similarity
Amino acid modifications
	Cross-link	98 ↔ 219		Tryptophyl-tyrosyl-methioninium (Trp-Tyr) (with M-245) By similarity
	Cross-link	219 ↔ 245		Tryptophyl-tyrosyl-methioninium (Tyr-Met) (with W-98) By similarity

Sequences

	Sequence		Length	Mass (Da)
	B0V4K1 [UniParc]. Last modified April 8, 2008. Version 1. Checksum: ADDE8973C4C0D0BA	FASTA	718	78,629
	10 20 30 40 50 60 MSNESKCPFS GHNSKPQVTV GGGTANLHWW PNQLRVDLLN QHSERSNPLG KDFNYRQEFK 70 80 90 100 110 120 KLDYYALKAD IKNVLTDSQD WWPADWGNYT GLFIRLAWHA AGTYRMGDGR GGAGRGQQRF 130 140 150 160 170 180 APLNSWPDNA SLDKARRLLW PVKQKYGQKI SWADLFILAG NIALESSGFR TFGFGAGRED 190 200 210 220 230 240 VWEPDNDVNW GDEKEWLAHR NSEALAGSNL AATEMGLIYV NPEGPQASGD PRSAAPFIRA 250 260 270 280 290 300 TFGNMAMDDE EIVALIAGGH TLGKTHGAAP ADHVQADPEG APIEQMGFGW ANSYGTGVGK 310 320 330 340 350 360 DAITSGLEVI WSQTPTQWSN YFFENLFKYE WVQERSPAGA IQWVAADAEA IIPDPFDPSI 370 380 390 400 410 420 KRKPTMLTTD LTLRFDPEFE KISRRFLNDP QAFANAFARA WFKLTHRDMG PKARYLGPEV 430 440 450 460 470 480 PTEDLIWQDP LPAASATPSS ASIADAKAKI VALGLPAGEL VSLAWASAST FRGGDKRGGA 490 500 510 520 530 540 NGARIALSPQ REWEVNKKAV ETLTKIEELK ASTQLSLADL IVLAGNVGVE QAAQAAGFNI 550 560 570 580 590 600 TVPFAPGRVD ALQSQTDVES FQLLLGLADG FRNWKKQGVN TPAEVLLIDK AQQLTLTAPE 610 620 630 640 650 660 LTALIGGLRV LGTNWDGSQH GVFTQQVGVL STDFFTNLLD MSNVWAPVDS TSEVFEGKDR 670 680 690 700 710 KSGTVKFTAT RNDLVFGSNS ILRALAEVYA QADGKEKFVQ DFVAAWTKVM NLDRFDLA

References

[1]

"Comparative analysis of Acinetobacters: three genomes for three lifestyles." Vallenet D., Nordmann P., Barbe V., Poirel L., Mangenot S., Bataille E., Dossat C., Gas S., Kreimeyer A., Lenoble P., Oztas S., Poulain J., Segurens B., Robert C., Abergel C., Claverie J.-M., Raoult D., Medigue C., Weissenbach J., Cruveiller S. PLoS ONE 3:E1805-E1805(2008) [PubMed: 18350144] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AYE.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CU459141 Genomic DNA. Translation: CAM88162.1.
RefSeq	YP_001715131.1. NC_010410.1.
3D structure databases
ProteinModelPortal	B0V4K1.
SMR	B0V4K1. Positions 23-718.
ModBase	Search...
Protein-protein interaction databases
STRING	B0V4K1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6003728.
GenomeReviews	Gene locus ABAYE3366 in contig CU459141_GR.
PATRIC	20729866. VBIAciBau69881_3230.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG285610.
OMA	KRHAPSM.
ProtClustDB	PRK15061.
Family and domain databases
HAMAP	MF_01961. Catal-peroxid. [Tree]
InterPro	IPR000763. Catalase_peroxidase. IPR010255. Haem_peroxidase. IPR002016. Haem_peroxidase_pln/fun/bac. IPR019794. Peroxidases_AS. [Graphical view]
Pfam	PF00141. peroxidase. 2 hits. [Graphical view]
PRINTS	PR00460. BPEROXIDASE. PR00458. PEROXIDASE.
SUPFAM	SSF48113. Peroxidase_super. 2 hits.
TIGRFAMs	TIGR00198. Cat_per_HPI. 1 hit.
PROSITE	PS00435. PEROXIDASE_1. False negative. PS00436. PEROXIDASE_2. 1 hit. PS50873. PEROXIDASE_4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KATG_ACIBY

Accession

Primary (citable) accession number: B0V4K1

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 25, 2008
Last sequence update:	April 8, 2008
Last modified:	January 25, 2012
This is version 26 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program