B0UWA9 (SYGB_HAES2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glycine--tRNA ligase beta subunit
EC=6.1.1.14

Alternative name(s):
Glycyl-tRNA synthetase beta subunit
Short name=GlyRS

Gene names

Name:	glyS
Ordered Locus Names:	HSM_1792

Organism

Haemophilus somnus (strain 2336) (Histophilus somni (strain 2336)) [Complete proteome] [HAMAP]

Taxonomic identifier

228400 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Histophilus › Haemophilus somnus

Protein attributes

Sequence length	688 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-tRNA(Gly). HAMAP-Rule MF_00255
Subunit structure	Tetramer of two alpha and two beta subunits By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	arginyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro glycyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP arginine-tRNA ligase activity Inferred from electronic annotation. Source: InterPro glycine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 688	688	Glycine--tRNA ligase beta subunit HAMAP-Rule MF_00255		PRO_1000078543

Sequences

	Sequence		Length	Mass (Da)
	B0UWA9 [UniParc]. Last modified April 8, 2008. Version 1. Checksum: FF8E89945D5061EF	FASTA	688	75,414
	10 20 30 40 50 60 MTAQNFLAEI GTEELPPKAL KKLATAFAEN VEQELNQAGL AFEKVEWFAA PRRLAVKVLG 70 80 90 100 110 120 LAEAQPSKQV EKRGPAVSAA FDAEGKPTKA AEGWARGCGI TVEQAERLAT DKGEWLVHRA 130 140 150 160 170 180 VIEGQPTKNL LVGIIDKALA GLPIPKTMRW GDKTEQFVRP VHTVTLLFGA DLIEGEILGV 190 200 210 220 230 240 ASGRTVRGHR FLGEREFSLD HADQYPQLLK ERGSVVADFN ERKALILATS QEKATALGGV 250 260 270 280 290 300 ADIEEELLEE VTSLVEYPNV LTAKFEERFL AVPAEALVYT MKGDQKYFPI YDKDGKLLPH 310 320 330 340 350 360 FIFVSNINPD DPSKIIEGNE KVVRPRLTDA EFFFKTDLKQ KLEDRLPRLE TVLFQQQLGT 370 380 390 400 410 420 LRDKTARIEA LAGEIAAQIG ADQTKAKRAG LLSKCDLMTN MVFEFTDTQG VMGMHYARHD 430 440 450 460 470 480 GEDEDVAVAL NEQYMPRFAG DTLPNSLVAC SVALADKIDT LTGIFGIGQS PKGSADPFAL 490 500 510 520 530 540 RRAALGCLRI IVEKNLPLDL VDIVAKATAL FGDKLTNKNV VDEVVDFMLG RFRAWYESEG 550 560 570 580 590 600 IAVDVIQSVL ARRPTKPADF DARVRAVSHF RTLDSAEALA AANKRVSNIL AKADIAIGEI 610 620 630 640 650 660 DLGVCVESAE KTLAEAVIAL KGEVQPLIAQ GDYTAVLDKL ANLRQPIDAF FDGVMVNVEE 670 680 QTLRQNRLAI LSTLQNLFLQ VADISVLQ

References

[1]

"Complete sequence of Haemophilus somnus 2336." US DOE Joint Genome Institute Siddaramappa S., Duncan A.J., Challacombe J.F., Rainey D., Gillaspy A.F., Carson M., Gipson J., Gipson M., Bruce D., Detter J.C., Han C.S., Land M., Tapia R., Thompson L.S., Orvis J., Zaitshik J., Barnes G., Brettin T.S., Dyer D.W., Inzana T.J. Submitted (FEB-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 2336.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000947 Genomic DNA. Translation: ACA31576.1.
RefSeq	YP_001785112.1. NC_010519.1.
3D structure databases
ProteinModelPortal	B0UWA9.
ModBase	Search...
Protein-protein interaction databases
STRING	228400.HSM_1792.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACA31576; ACA31576; HSM_1792.
GeneID	6107713.
KEGG	hsm:HSM_1792.
PATRIC	20200850. VBIHaeSom93646_1888.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0751.
HOGENOM	HOG000264302.
KO	K01879.
OMA	GMHYARL.
ProtClustDB	PRK01233.
Enzyme and pathway databases
BioCyc	HSOM228400:GHWT-876-MONOMER.
Family and domain databases
HAMAP	MF_00255. Gly_tRNA_synth_beta.
InterPro	IPR008909. DALR_anticod-bd. IPR015944. Gly-tRNA-synth_bsu. IPR006194. Gly-tRNA-synth_heterodimer. [Graphical view]
Pfam	PF05746. DALR_1. 1 hit. PF02092. tRNA_synt_2f. 1 hit. [Graphical view]
PRINTS	PR01045. TRNASYNTHGB.
SMART	SM00836. DALR_1. 1 hit. [Graphical view]
TIGRFAMs	TIGR00211. glyS. 1 hit.
PROSITE	PS50861. AA_TRNA_LIGASE_II_GLYAB. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYGB_HAES2

Accession

Primary (citable) accession number: B0UWA9

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	April 8, 2008
Last modified:	May 1, 2013
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program