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Protein

Argininosuccinate synthase

Gene

argG

Organism
Histophilus somni (strain 2336) (Haemophilus somnus)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-citrulline + L-aspartate = AMP + diphosphate + N(omega)-(L-arginino)succinate.UniRule annotation

Pathwayi: L-arginine biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes L-arginine from L-ornithine and carbamoyl phosphate.UniRule annotation
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Argininosuccinate synthase (argG)
  3. Argininosuccinate lyase (argH)
This subpathway is part of the pathway L-arginine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-arginine from L-ornithine and carbamoyl phosphate, the pathway L-arginine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei44ATP; via amide nitrogen and carbonyl oxygenUniRule annotation1
Binding sitei100CitrullineUniRule annotation1
Binding sitei130ATP; via amide nitrogenUniRule annotation1
Binding sitei132AspartateUniRule annotation1
Binding sitei132ATPUniRule annotation1
Binding sitei136AspartateUniRule annotation1
Binding sitei136CitrullineUniRule annotation1
Binding sitei137AspartateUniRule annotation1
Binding sitei137ATPUniRule annotation1
Binding sitei140CitrullineUniRule annotation1
Binding sitei193CitrullineUniRule annotation1
Binding sitei195ATPUniRule annotation1
Binding sitei202CitrullineUniRule annotation1
Binding sitei204CitrullineUniRule annotation1
Binding sitei281CitrullineUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi18 – 26ATPUniRule annotation9

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Amino-acid biosynthesis, Arginine biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00068; UER00113.

Names & Taxonomyi

Protein namesi
Recommended name:
Argininosuccinate synthaseUniRule annotation (EC:6.3.4.5UniRule annotation)
Alternative name(s):
Citrulline--aspartate ligaseUniRule annotation
Gene namesi
Name:argGUniRule annotation
Ordered Locus Names:HSM_0253
OrganismiHistophilus somni (strain 2336) (Haemophilus somnus)
Taxonomic identifieri228400 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHistophilus
Proteomesi
  • UP000008543 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000824011 – 444Argininosuccinate synthaseAdd BLAST444

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliB0UW58.
SMRiB0UW58.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the argininosuccinate synthase family. Type 2 subfamily.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000230094.
KOiK01940.
OMAiAFHIRSG.
OrthoDBiPOG091H01U0.

Family and domain databases

Gene3Di1.10.287.400. 1 hit.
3.40.50.620. 1 hit.
3.90.1260.10. 1 hit.
HAMAPiMF_00581. Arg_succ_synth_type2. 1 hit.
InterProiIPR023437. Arg_succ_synth_type2_subfam.
IPR001518. Arginosuc_synth.
IPR018223. Arginosuc_synth_CS.
IPR024074. AS_cat/multimer_dom_body.
IPR024073. AS_multimer_C_tail.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00764. Arginosuc_synth. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00032. argG. 1 hit.
PROSITEiPS00564. ARGININOSUCCIN_SYN_1. 1 hit.
PS00565. ARGININOSUCCIN_SYN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B0UW58-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSNTILQHLP IGQKVGIAFS GGLDTSAALL WMRQKGAVPY AYTANLGQPD
60 70 80 90 100
EEDYNAIPRK AMEYGAEKAR LIDCRNQLAH EGIAAIQCGA FHISTGGVTY
110 120 130 140 150
FNTTPLGRAV TGTMLVSAMK EDDVNIWGDG STFKGNDIER FYRYGLLTNP
160 170 180 190 200
SLRIYKPWLD NQFIDELGGR QEMSEFLIAN GFDYKMSAEK AYSTDSNMLG
210 220 230 240 250
ATHEAKDLEY LNSGIRIVKP IMGVAFWRDD VTVKAEEVTV GFEDGVPVTL
260 270 280 290 300
NGQSFSSAVE LFLEANRIGG RHGLGMSDQI ENRIIEAKSR GIYEAPGMAL
310 320 330 340 350
LHIAYERLVT AIHNEDTIEQ YRINGLRLGR LLYQGRWFDP QALMLRETAQ
360 370 380 390 400
RWVARAVTGE VTLELRRGND YSILNTVSPN MTYHPERLSM EKVENAPFDP
410 420 430 440
SDRIGQLTMR NLDITDTRDK LGIYTHTGLL TVGKDSMLPQ LDKK
Length:444
Mass (Da):49,607
Last modified:April 8, 2008 - v1
Checksum:iE1009DA7889C0D5E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000947 Genomic DNA. Translation: ACA31880.1.
RefSeqiWP_012341124.1. NC_010519.1.

Genome annotation databases

EnsemblBacteriaiACA31880; ACA31880; HSM_0253.
KEGGihsm:HSM_0253.
PATRICi20197503. VBIHaeSom93646_0265.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000947 Genomic DNA. Translation: ACA31880.1.
RefSeqiWP_012341124.1. NC_010519.1.

3D structure databases

ProteinModelPortaliB0UW58.
SMRiB0UW58.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACA31880; ACA31880; HSM_0253.
KEGGihsm:HSM_0253.
PATRICi20197503. VBIHaeSom93646_0265.

Phylogenomic databases

HOGENOMiHOG000230094.
KOiK01940.
OMAiAFHIRSG.
OrthoDBiPOG091H01U0.

Enzyme and pathway databases

UniPathwayiUPA00068; UER00113.

Family and domain databases

Gene3Di1.10.287.400. 1 hit.
3.40.50.620. 1 hit.
3.90.1260.10. 1 hit.
HAMAPiMF_00581. Arg_succ_synth_type2. 1 hit.
InterProiIPR023437. Arg_succ_synth_type2_subfam.
IPR001518. Arginosuc_synth.
IPR018223. Arginosuc_synth_CS.
IPR024074. AS_cat/multimer_dom_body.
IPR024073. AS_multimer_C_tail.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00764. Arginosuc_synth. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00032. argG. 1 hit.
PROSITEiPS00564. ARGININOSUCCIN_SYN_1. 1 hit.
PS00565. ARGININOSUCCIN_SYN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiASSY_HISS2
AccessioniPrimary (citable) accession number: B0UW58
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: April 8, 2008
Last modified: November 2, 2016
This is version 57 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.