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B0UV12

- SYI_HISS2

UniProt

B0UV12 - SYI_HISS2

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Protein
Isoleucine--tRNA ligase
Gene
ileS, HSM_0054
Organism
Histophilus somni (strain 2336) (Haemophilus somnus)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei561 – 5611Aminoacyl-adenylate By similarity
Binding sitei605 – 6051ATP By similarity
Metal bindingi900 – 9001Zinc By similarity
Metal bindingi903 – 9031Zinc By similarity
Metal bindingi920 – 9201Zinc By similarity
Metal bindingi923 – 9231Zinc By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. aminoacyl-tRNA editing activity Source: InterPro
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciHSOM228400:GHWT-54-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:HSM_0054
OrganismiHistophilus somni (strain 2336) (Haemophilus somnus)
Taxonomic identifieri228400 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHistophilus
ProteomesiUP000008543: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 937937Isoleucine--tRNA ligaseUniRule annotation
PRO_1000088547Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi228400.HSM_0054.

Structurei

3D structure databases

ProteinModelPortaliB0UV12.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi58 – 6811"HIGH" regionUniRule annotation
Add
BLAST
Motifi602 – 6065"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B0UV12-1 [UniParc]FASTAAdd to Basket

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MTDYKNTLNL PETGFPMRGD LAKREPDMLK NWYDKNLYQK VREASKGKKT    50
FILHDGPPYA NGTLHLGHAV NKILKDIIMK SKTALGFDTP YVPGWDCHGL 100
PIELKVEGLV GKPNEKISAA EFRQACRDYA KEQVEGQKAD FIRMGVLGDW 150
DNPYLTMNFE TEAEIIRTLG KVIQNGHLYK GSKPVHWCLD CASSLAEAEV 200
EYEDKVSPSI YVRFAAVSAA EVEEKFNALG KGQGALSAVI WTTTPWTLPS 250
NRAIAVNAEL EYQLVQLGDE RVILAAELVQ AVQNALKIEQ LEILGSTTGQ 300
DLELVRFHHP FYDFSVPIIL GDHVTVDGGT GLVHTAPDHG QDDFVVSKKY 350
GLEMAGLVAN DGKFISSTPF FAGKGVFEAN DLVLEKLKET GALLKLERIK 400
HSYPHCWRHK TPIIFRATPQ WFIGMETQDL RIKALSEIKS VRWIPSWGEA 450
RIDKMVANRP DWCISRQRTW GVPMTMFVHN ETEELHPRTL ELLEDIAKRV 500
EKAGIQAWWD LDPAELLGED AKTYHKVPDT LDVWFDSGST YASVVEQRPE 550
FNGKSTDMYL EGSDQHRGWF MSSLMLSTAT NNKAPYKQVL THGFTVDEKG 600
RKMSKSLGNV IVPSEVWNKN GADILRLWVA STDYTGEIAV SHKILNSAGD 650
TYRRIRNTAR FLLANLNGFD PKNDLVNPEE MISLDRWAVS CALEAQNEIK 700
EAYDNYQFHT VVQRLMRFCS IEMGSFYLDI IKDRQYTTKA DSLARRSCQT 750
ALWHISEALV RWIAPILSFT ADEIWGYLPK LDNRAEFVFT EEFYDGLFGL 800
DESDKLDDTY WQQLLKVRAE VNRVLEQARN DKLIGAGLEA KVTVYASEEI 850
RPLLEQLGNE LRFVLITSQV VVKPLSKADV AESELTGLAI KVERADGEKC 900
PRCWHFSTDI GSNKEHSHIC GRCIENVEGN GEQRQFA 937
Length:937
Mass (Da):105,956
Last modified:April 8, 2008 - v1
Checksum:i260FD415D7822660
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000947 Genomic DNA. Translation: ACA32189.1.
RefSeqiYP_001783409.1. NC_010519.1.

Genome annotation databases

EnsemblBacteriaiACA32189; ACA32189; HSM_0054.
GeneIDi6108324.
KEGGihsm:HSM_0054.
PATRICi20197065. VBIHaeSom93646_0055.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000947 Genomic DNA. Translation: ACA32189.1 .
RefSeqi YP_001783409.1. NC_010519.1.

3D structure databases

ProteinModelPortali B0UV12.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 228400.HSM_0054.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACA32189 ; ACA32189 ; HSM_0054 .
GeneIDi 6108324.
KEGGi hsm:HSM_0054.
PATRICi 20197065. VBIHaeSom93646_0055.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci HSOM228400:GHWT-54-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 2336.

Entry informationi

Entry nameiSYI_HISS2
AccessioniPrimary (citable) accession number: B0UV12
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: April 8, 2008
Last modified: May 14, 2014
This is version 48 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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