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B0UU89 (GLGB_HISS2) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 49. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
1,4-alpha-glucan branching enzyme GlgB

EC=2.4.1.18
Alternative name(s):
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase
Alpha-(1->4)-glucan branching enzyme
Glycogen branching enzyme
Short name=BE
Gene names
Name:glgB
Ordered Locus Names:HSM_1368
OrganismHistophilus somni (strain 2336) (Haemophilus somnus) [Complete proteome] [HAMAP]
Taxonomic identifier228400 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHistophilus

Protein attributes

Sequence length815 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position By similarity. HAMAP-Rule MF_00685

Catalytic activity

Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain. HAMAP-Rule MF_00685

Pathway

Glycan biosynthesis; glycogen biosynthesis. HAMAP-Rule MF_00685

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00685

Sequence similarities

Belongs to the glycosyl hydrolase 13 family. GlgB subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 8158151,4-alpha-glucan branching enzyme GlgB HAMAP-Rule MF_00685
PRO_1000083068

Sites

Active site4051Nucleophile By similarity
Active site4581Proton donor By similarity

Sequences

Sequence LengthMass (Da)Tools
B0UU89 [UniParc].

Last modified April 8, 2008. Version 1.
Checksum: 744431EF7F2527B3

FASTA81593,617
        10         20         30         40         50         60 
MNKFVSQSTI DTFFDGEHAD PFSVLGMHET SNGIEVRVLL PDAEKVFVLS KETKNVLCEL 

        70         80         90        100        110        120 
LRVDERGFFA GVVPNTHSFF AYQLEVYWGN EAQVIEDPYA FHPMINELDN WLLAEGSHKR 

       130        140        150        160        170        180 
PYEILGAHFT ECDNVAGVNF RLWAPNAKRV SVVGDFNYWD GRRHPMRFHQ SSGIWELFLP 

       190        200        210        220        230        240 
KVSLGQLYKF ELLDCHNQLR LKADPYAFSS QLRPDTASQI GALPEIVSMT EKRRKANQAD 

       250        260        270        280        290        300 
QPISIYEVHL GSWRRNLENN FWLDYDQIAE ELIPYVKDMG FTHIELLPLS EFPFDGSWGY 

       310        320        330        340        350        360 
QPIGLYSPTS RFGTAEGFKR LVNKAHKAGI NVILDWVPGH FPSDTHGLVA FDGTALYEHA 

       370        380        390        400        410        420 
DPKEGYHQDW NTLIYNYGRH EVKNYLASNA LYWMERFGLD GIRVDAVASM IYRDYSREDG 

       430        440        450        460        470        480 
QWIPNQYGGR ENLEAIEFLK QTNHLLGTEI PGVVSIAEES TSFAGVTHPP YEGGLGFHFK 

       490        500        510        520        530        540 
WNMGWMNDTL SYMKKDPIYR QHHHSQMTFG MVYQYSENFI LPLSHDEVVH GKCSLLGKMP 

       550        560        570        580        590        600 
GDAWQKFANL RAYYGYMWGY PGKKLLFMGN EFAQGREWNY QESLDWYLLD EFHGGGWHKA 

       610        620        630        640        650        660 
IQDYVRDLNH IYQKNAPLFE LDTDPQGFEW LVVDDYQNSV FVFERRSKKD ERIIVVSNFT 

       670        680        690        700        710        720 
PVLRQNYRFG VNIAGEYLEI LNSDAQQYMG SNSTNTSKIV TEDIESHNKA QSISIDIPPL 

       730        740        750        760        770        780 
ATVYLKLHKV KKVRKMRKTS KVEDTAVKTE KKIAKGKTTR TKKTVADTVS EATEVKPKKT 

       790        800        810 
VTKRAVVKKV KNEESAVFPN AEVIAESTSV ENNVS 

« Hide

References

[1]"Complete sequence of Haemophilus somnus 2336."
US DOE Joint Genome Institute
Siddaramappa S., Duncan A.J., Challacombe J.F., Rainey D., Gillaspy A.F., Carson M., Gipson J., Gipson M., Bruce D., Detter J.C., Han C.S., Land M., Tapia R., Thompson L.S., Orvis J., Zaitshik J., Barnes G., Brettin T.S., Dyer D.W., Inzana T.J.
Submitted (FEB-2008) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 2336.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000947 Genomic DNA. Translation: ACA31105.1.
RefSeqYP_001784688.1. NC_010519.1.

3D structure databases

ProteinModelPortalB0UU89.
SMRB0UU89. Positions 117-725.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING228400.HSM_1368.

Protein family/group databases

CAZyCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACA31105; ACA31105; HSM_1368.
GeneID6107242.
KEGGhsm:HSM_1368.
PATRIC20199946. VBIHaeSom93646_1442.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0296.
HOGENOMHOG000283037.
KOK00700.
OrthoDBEOG6JX7GT.

Enzyme and pathway databases

BioCycHSOM228400:GHWT-1403-MONOMER.
UniPathwayUPA00164.

Family and domain databases

Gene3D2.60.40.10. 2 hits.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
HAMAPMF_00685. GlgB.
InterProIPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERPTHR10357. PTHR10357. 1 hit.
PfamPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFPIRSF000463. GlgB. 1 hit.
SUPFAMSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 2 hits.
TIGRFAMsTIGR01515. branching_enzym. 1 hit.
ProtoNetSearch...

Entry information

Entry nameGLGB_HISS2
AccessionPrimary (citable) accession number: B0UU89
Entry history
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: April 8, 2008
Last modified: June 11, 2014
This is version 49 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries