B0UU16 (METE_HAES2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase
EC=2.1.1.14

Alternative name(s):
Cobalamin-independent methionine synthase
Methionine synthase, vitamin-B12 independent isozyme

Gene names

Name:	metE
Ordered Locus Names:	HSM_1293

Organism

Haemophilus somnus (strain 2336) (Histophilus somni (strain 2336)) [Complete proteome] [HAMAP]

Taxonomic identifier

228400 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Histophilus

Protein attributes

Sequence length	757 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the transfer of a methyl group from 5-methyltetrahydrofolate to homocysteine resulting in methionine formation By similarity. HAMAP MF_00172
Catalytic activity	5-methyltetrahydropteroyltri-L-glutamate + L-homocysteine = tetrahydropteroyltri-L-glutamate + L-methionine. HAMAP MF_00172
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00172
Pathway	Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-methionine from L-homocysteine (MetE route): step 1/1. HAMAP MF_00172
Sequence similarities	Belongs to the vitamin-B12 independent methionine synthase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Methionine biosynthesis
Domain	Repeat
Ligand	Metal-binding Zinc
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	methionine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 757	757	5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase HAMAP MF_00172		PRO_1000077114
Sites
	Metal binding	643	1	Zinc By similarity
	Metal binding	645	1	Zinc By similarity
	Metal binding	728	1	Zinc By similarity

Sequences

	Sequence		Length	Mass (Da)
	B0UU16 [UniParc]. Last modified April 8, 2008. Version 1. Checksum: 0E074374666F91ED	FASTA	757	85,893
	10 20 30 40 50 60 MTTFHVLGFP RVGAKRELKF AQERYWRKEL AEQDLLDLAK ALREKNWKHQ ADANADFMAV 70 80 90 100 110 120 GDFTFYDHIL DLQVATGAIP ARFGFDSKNL TLDQYFQLAR GNKDQFAIEM TKWFDTNYHY 130 140 150 160 170 180 LVPEFHKDTQ FKANPTHYVN QIREAKVAGH QVKPVIVGPL TFLWLGKEKG ESFNRFELLA 190 200 210 220 230 240 QLVPVYVEIL NALVAEGTEW IQIDEPALAV DLPKEWVNAY KDVYATLSEK VNAKLLLATY 250 260 270 280 290 300 FGSVTEHATL LKELPVDGLH LDLVRAPEQL VAFEDYNKVL SAGVIDGRNI WRANLNTVLD 310 320 330 340 350 360 VLEPLKEKLG ERLWIAPSCS LLHTPFDLNV ETQLQENNPA LYSWLAFTLQ KVQELSVLKT 370 380 390 400 410 420 ALEKGRNAVN VELDASQVAA DARANSKEIH RPEVAERLAN LPKNADQRKS PFGERIKLQN 430 440 450 460 470 480 AWLNLPLLPT TSIGSFPQTT EIRRARAAFK KGDLSLEEYE NSMKKEIELV VREQEKLDLD 490 500 510 520 530 540 VLVHGEPERN DMVEYFGELL DGFAFTKFGW VQSYGSRCVK PPVIYGDVVR PEPMTVRWSK 550 560 570 580 590 600 YAQSLTKKVM KGMLTGPVTI LQWSFVRNDI PRSTVCKQIG VALSDEVLDL EKAGIKVIQI 610 620 630 640 650 660 DEPAIREGLP LKRADWDTYL QWAGEAFRLS SMGVADDTQI HTHMCYSEFN DILPAIAALD 670 680 690 700 710 720 ADVITIETSR SDMELLTAFG DFKYPNDIGP GVYDIHSPRV PTAEEIEHLL RKALNVVPKE 730 740 750 RLWVNPDCGL KTRGWSETIA QLEVMMSVTK KLRAELA

References

[1]

"Complete sequence of Haemophilus somnus 2336." US DOE Joint Genome Institute Siddaramappa S., Duncan A.J., Challacombe J.F., Rainey D., Gillaspy A.F., Carson M., Gipson J., Gipson M., Bruce D., Detter J.C., Han C.S., Land M., Tapia R., Thompson L.S., Orvis J., Zaitshik J., Barnes G., Brettin T.S., Dyer D.W., Inzana T.J. Submitted (FEB-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 2336.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000947 Genomic DNA. Translation: ACA31024.1.
RefSeq	YP_001784615.1. NC_010519.1.
3D structure databases
ProteinModelPortal	B0UU16.
ModBase	Search...
Protein-protein interaction databases
STRING	B0UU16.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6107160.
GenomeReviews	Gene locus HSM_1293 in contig CP000947_GR.
KEGG	hsm:HSM_1293.
PATRIC	20199794. VBIHaeSom93646_1366.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG287495.
OMA	RNIWRAN.
ProtClustDB	PRK05222.
Enzyme and pathway databases
BioCyc	HSOM228400:HSM_1293-MONOMER.
Family and domain databases
HAMAP	MF_00172. Meth_synth. [Tree]
InterPro	IPR013215. Cbl-indep_Met_Synth_N. IPR006276. Cobalamin-indep_Met_synthase. IPR002629. Methionine_synth. [Graphical view]
KO	K00549.
Pfam	PF08267. Meth_synt_1. 1 hit. PF01717. Meth_synt_2. 1 hit. [Graphical view]
PIRSF	PIRSF000382. MeTrfase_B12_ind. 1 hit.
TIGRFAMs	TIGR01371. Met_syn_B12ind. 1 hit.
ProtoNet	Search...

Entry information

Entry name

METE_HAES2

Accession

Primary (citable) accession number: B0UU16

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	April 8, 2008
Last modified:	January 25, 2012
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program