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B0UTC6 (NAGZ_HISS2) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 43. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Beta-hexosaminidase

EC=3.2.1.52
Alternative name(s):
Beta-N-acetylhexosaminidase
N-acetyl-beta-glucosaminidase
Gene names
Name:nagZ
Ordered Locus Names:HSM_1045
OrganismHistophilus somni (strain 2336) (Haemophilus somnus) [Complete proteome] [HAMAP]
Taxonomic identifier228400 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHistophilus

Protein attributes

Sequence length348 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Plays a role in peptidoglycan recycling by cleaving the terminal beta-1,4-linked N-acetylglucosamine (GlcNAc) from peptide-linked peptidoglycan fragments, giving rise to free GlcNAc, anhydro-N-acetylmuramic acid and anhydro-N-acetylmuramic acid-linked peptides By similarity. HAMAP-Rule MF_00364

Catalytic activity

Hydrolysis of terminal non-reducing N-acetyl-D-hexosamine residues in N-acetyl-beta-D-hexosaminides. HAMAP-Rule MF_00364

Pathway

Cell wall biogenesis; peptidoglycan recycling. HAMAP-Rule MF_00364

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00364.

Sequence similarities

Belongs to the glycosyl hydrolase 3 family. NagZ subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 348348Beta-hexosaminidase HAMAP-Rule MF_00364
PRO_1000079575

Regions

Region164 – 1652Substrate binding By similarity

Sites

Active site1771Proton donor/acceptor By similarity
Active site2491Nucleophile By similarity
Binding site621Substrate By similarity
Binding site701Substrate By similarity
Binding site1341Substrate By similarity
Site1751Important for catalytic activity By similarity

Sequences

Sequence LengthMass (Da)Tools
B0UTC6 [UniParc].

Last modified April 8, 2008. Version 1.
Checksum: D7636FC113199C34

FASTA34839,887
        10         20         30         40         50         60 
MSILLIDLSG QELRQEEIEL LEHPLVAGLI LFSRNFYDIE QIRHLIRSVR QKVKKPLLIT 

        70         80         90        100        110        120 
VDQEGGRVQR FRQGLTQLPA MQSFACLLSD PQEQQEMAWR AGWQMAAEMT ALDIDLSFAP 

       130        140        150        160        170        180 
VLDLGHQCKA IGDRSFHYEE KKLIELAEKF IQGMRQIGMS ATGKHFPGHG HVLADSHLET 

       190        200        210        220        230        240 
PYDDRAKELI FAQDIRPFQS LIKQGLLDAV MPAHVIYTQC DNQPASGSSY WLKEVLRQQL 

       250        260        270        280        290        300 
GFQGAIFSDD LGMKGAGFMG DFVARCTQSL QAGCDLLLLC NEPEAVVQVL DRFKPQESQN 

       310        320        330        340 
KRIIRQTRLN KLFKKQRIDW QTLRTQRDWL ENHKKLTALQ QDWLAYKG 

« Hide

References

[1]"Complete sequence of Haemophilus somnus 2336."
US DOE Joint Genome Institute
Siddaramappa S., Duncan A.J., Challacombe J.F., Rainey D., Gillaspy A.F., Carson M., Gipson J., Gipson M., Bruce D., Detter J.C., Han C.S., Land M., Tapia R., Thompson L.S., Orvis J., Zaitshik J., Barnes G., Brettin T.S., Dyer D.W., Inzana T.J.
Submitted (FEB-2008) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 2336.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000947 Genomic DNA. Translation: ACA30759.1.
RefSeqYP_001784375.1. NC_010519.1.

3D structure databases

ProteinModelPortalB0UTC6.
SMRB0UTC6. Positions 2-301.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING228400.HSM_1045.

Protein family/group databases

CAZyGH3. Glycoside Hydrolase Family 3.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACA30759; ACA30759; HSM_1045.
GeneID6106890.
KEGGhsm:HSM_1045.
PATRIC20199275. VBIHaeSom93646_1112.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1472.
HOGENOMHOG000248526.
KOK01207.
OrthoDBEOG6BCT06.

Enzyme and pathway databases

BioCycHSOM228400:GHWT-1080-MONOMER.
UniPathwayUPA00544.

Family and domain databases

Gene3D3.20.20.300. 1 hit.
HAMAPMF_00364. NagZ.
InterProIPR022956. Beta_hexosaminidase_bac.
IPR001764. Glyco_hydro_3_N.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamPF00933. Glyco_hydro_3. 1 hit.
[Graphical view]
SUPFAMSSF51445. SSF51445. 1 hit.
ProtoNetSearch...

Entry information

Entry nameNAGZ_HISS2
AccessionPrimary (citable) accession number: B0UTC6
Entry history
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: April 8, 2008
Last modified: May 14, 2014
This is version 43 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries