B0UTC6 (NAGZ_HAES2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Beta-hexosaminidase
EC=3.2.1.52

Alternative name(s):
Beta-N-acetylhexosaminidase
N-acetyl-beta-glucosaminidase

Gene names

Name:	nagZ
Ordered Locus Names:	HSM_1045

Organism

Haemophilus somnus (strain 2336) (Histophilus somni (strain 2336)) [Complete proteome] [HAMAP]

Taxonomic identifier

228400 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Histophilus › Haemophilus somnus

Protein attributes

Sequence length	348 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Cleaves GlcNAc linked beta-1,4 to MurNAc tripeptides By similarity. HAMAP-Rule MF_00364
Catalytic activity	Hydrolysis of terminal non-reducing N-acetyl-D-hexosamine residues in N-acetyl-beta-D-hexosaminides. HAMAP-Rule MF_00364
Pathway	Cell wall biogenesis; peptidoglycan recycling. HAMAP-Rule MF_00364
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the glycosyl hydrolase 3 family. NagZ subfamily.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Molecular function	Glycosidase Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan turnover Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	beta-N-acetylhexosaminidase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 348	348	Beta-hexosaminidase HAMAP-Rule MF_00364		PRO_1000079575
Sites
	Active site	249	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	B0UTC6 [UniParc]. Last modified April 8, 2008. Version 1. Checksum: D7636FC113199C34	FASTA	348	39,887
	10 20 30 40 50 60 MSILLIDLSG QELRQEEIEL LEHPLVAGLI LFSRNFYDIE QIRHLIRSVR QKVKKPLLIT 70 80 90 100 110 120 VDQEGGRVQR FRQGLTQLPA MQSFACLLSD PQEQQEMAWR AGWQMAAEMT ALDIDLSFAP 130 140 150 160 170 180 VLDLGHQCKA IGDRSFHYEE KKLIELAEKF IQGMRQIGMS ATGKHFPGHG HVLADSHLET 190 200 210 220 230 240 PYDDRAKELI FAQDIRPFQS LIKQGLLDAV MPAHVIYTQC DNQPASGSSY WLKEVLRQQL 250 260 270 280 290 300 GFQGAIFSDD LGMKGAGFMG DFVARCTQSL QAGCDLLLLC NEPEAVVQVL DRFKPQESQN 310 320 330 340 KRIIRQTRLN KLFKKQRIDW QTLRTQRDWL ENHKKLTALQ QDWLAYKG

References

[1]

"Complete sequence of Haemophilus somnus 2336." US DOE Joint Genome Institute Siddaramappa S., Duncan A.J., Challacombe J.F., Rainey D., Gillaspy A.F., Carson M., Gipson J., Gipson M., Bruce D., Detter J.C., Han C.S., Land M., Tapia R., Thompson L.S., Orvis J., Zaitshik J., Barnes G., Brettin T.S., Dyer D.W., Inzana T.J. Submitted (FEB-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 2336.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000947 Genomic DNA. Translation: ACA30759.1.
RefSeq	YP_001784375.1. NC_010519.1.
3D structure databases
ProteinModelPortal	B0UTC6.
SMR	B0UTC6. Positions 2-301.
ModBase	Search...
Protein-protein interaction databases
STRING	228400.HSM_1045.
Protein family/group databases
CAZy	GH3. Glycoside Hydrolase Family 3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACA30759; ACA30759; HSM_1045.
GeneID	6106890.
KEGG	hsm:HSM_1045.
PATRIC	20199275. VBIHaeSom93646_1112.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1472.
HOGENOM	HOG000248526.
KO	K01207.
OMA	VVLHCNG.
ProtClustDB	PRK05337.
Enzyme and pathway databases
BioCyc	HSOM228400:GHWT-53-MONOMER.
UniPathway	UPA00544.
Family and domain databases
Gene3D	3.20.20.300. 1 hit.
HAMAP	MF_00364. NagZ.
InterPro	IPR022956. Beta_hexosaminidase_bac. IPR001764. Glyco_hydro_3_N. IPR017853. Glycoside_hydrolase_SF. [Graphical view]
Pfam	PF00933. Glyco_hydro_3. 1 hit. [Graphical view]
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit.
PROSITE	PS00775. GLYCOSYL_HYDROL_F3. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NAGZ_HAES2

Accession

Primary (citable) accession number: B0UTC6

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	April 8, 2008
Last modified:	May 1, 2013
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program