B0US32 (ASNA_HAES2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Aspartate--ammonia ligase
EC=6.3.1.1

Alternative name(s):
Asparagine synthetase A

Gene names

Name:	asnA
Ordered Locus Names:	HSM_0591

Organism

Haemophilus somnus (strain 2336) (Histophilus somni (strain 2336)) [Complete proteome] [HAMAP]

Taxonomic identifier

228400 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Histophilus

Protein attributes

Sequence length	330 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-aspartate + NH3 = AMP + diphosphate + L-asparagine. HAMAP MF_00555
Pathway	Amino-acid biosynthesis; L-asparagine biosynthesis; L-asparagine from L-aspartate (ammonia route): step 1/1. HAMAP MF_00555
Subcellular location	Cytoplasm By similarity HAMAP MF_00555.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. AsnA subfamily.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Asparagine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	asparagine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW tRNA aminoacylation for protein translation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW aminoacyl-tRNA ligase activity Inferred from electronic annotation. Source: InterPro aspartate-ammonia ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 330	330	Aspartate--ammonia ligase HAMAP MF_00555		PRO_1000081982

Sequences

	Sequence		Length	Mass (Da)
	B0US32 [UniParc]. Last modified April 8, 2008. Version 1. Checksum: 1AA2D6611FE16D69	FASTA	330	37,610
	10 20 30 40 50 60 MKKSFILQQQ EISFAKNTFT EKLIEHLGII EVQGPILSQV GNGIQDNLSG TEKAVQVNVK 70 80 90 100 110 120 QITDAKFEVV HSLAKWKRHT LARFNFAENE GLFVHMKALR PDEDSLDQTH SVYVDQWDWE 130 140 150 160 170 180 KVIPTGRRNL AYLKETVRSI YQAILETEDA VHQKFGLSKF LPREITFIHS EELVQRYPEL 190 200 210 220 230 240 NDKQRENAIC KEYGAVFLMG IGGVLSDGKP HDKRAPDYDD WTTPSEGEYL GLNGDILVWN 250 260 270 280 290 300 PVLERAFEVS SMGIRVDETA LRKQLALTGD EDRLQFDWHQ DLVNGRLPLS IGGGIGQSRL 310 320 330 AMLLLQKKHI GEVQSSVWPK VVTEQFENIL

References

[1]

"Complete sequence of Haemophilus somnus 2336." US DOE Joint Genome Institute Siddaramappa S., Duncan A.J., Challacombe J.F., Rainey D., Gillaspy A.F., Carson M., Gipson J., Gipson M., Bruce D., Detter J.C., Han C.S., Land M., Tapia R., Thompson L.S., Orvis J., Zaitshik J., Barnes G., Brettin T.S., Dyer D.W., Inzana T.J. Submitted (FEB-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 2336.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000947 Genomic DNA. Translation: ACA32244.1.
RefSeq	YP_001783931.1. NC_010519.1.
3D structure databases
ProteinModelPortal	B0US32.
SMR	B0US32. Positions 4-330.
ModBase	Search...
Protein-protein interaction databases
STRING	B0US32.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6108389.
GenomeReviews	Gene locus HSM_0591 in contig CP000947_GR.
KEGG	hsm:HSM_0591.
PATRIC	20198250. VBIHaeSom93646_0617.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG288146.
OMA	LNDNLNG.
ProtClustDB	PRK05425.
Enzyme and pathway databases
BioCyc	HSOM228400:HSM_0591-MONOMER.
Family and domain databases
HAMAP	MF_00555. AsnA. [Tree]
InterPro	IPR006195. aa-tRNA-synth_II. IPR004618. AsnA. [Graphical view]
KO	K01914.
Pfam	PF03590. AsnA. 1 hit. [Graphical view]
PIRSF	PIRSF001555. Asp_ammon_ligase. 1 hit.
TIGRFAMs	TIGR00669. AsnA. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ASNA_HAES2

Accession

Primary (citable) accession number: B0US32

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	April 8, 2008
Last modified:	January 25, 2012
This is version 24 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program