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B0URQ3

- NAPA_HISS2

UniProt

B0URQ3 - NAPA_HISS2

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Protein

Periplasmic nitrate reductase

Gene

napA

Organism
Histophilus somni (strain 2336) (Haemophilus somnus)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein NapC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism.UniRule annotation

Catalytic activityi

Nitrite + acceptor = nitrate + reduced acceptor.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • [4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster.UniRule annotation
  • Mo-bis(molybdopterin guanine dinucleotide)UniRule annotationNote: Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi44 – 441Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi47 – 471Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi51 – 511Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi79 – 791Iron-sulfur (4Fe-4S)UniRule annotation

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
  2. electron carrier activity Source: UniProtKB-HAMAP
  3. iron ion binding Source: UniProtKB-HAMAP
  4. molybdenum ion binding Source: InterPro
  5. nitrate reductase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. Mo-molybdopterin cofactor biosynthetic process Source: UniProtKB-HAMAP
  2. nitrate assimilation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Nitrate assimilation, Transport

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, Molybdenum

Enzyme and pathway databases

BioCyciHSOM228400:GHWT-505-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Periplasmic nitrate reductaseUniRule annotation (EC:1.7.99.4UniRule annotation)
Gene namesi
Name:napAUniRule annotation
Ordered Locus Names:HSM_0493
OrganismiHistophilus somni (strain 2336) (Haemophilus somnus)
Taxonomic identifieri228400 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHistophilus
ProteomesiUP000008543: Chromosome

Subcellular locationi

Periplasm UniRule annotation

GO - Cellular componenti

  1. periplasmic space Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3232Tat-type signalUniRule annotationAdd
BLAST
Chaini33 – 827795Periplasmic nitrate reductasePRO_5000310961Add
BLAST

Post-translational modificationi

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.

Interactioni

Subunit structurei

Interacts with NapB.UniRule annotation

Protein-protein interaction databases

STRINGi228400.HSM_0493.

Structurei

3D structure databases

ProteinModelPortaliB0URQ3.
SMRiB0URQ3. Positions 36-826.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini37 – 93574Fe-4S Mo/W bis-MGD-typeUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily.UniRule annotation
Contains 1 4Fe-4S Mo/W bis-MGD-type domain.UniRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG0243.
HOGENOMiHOG000031441.
KOiK02567.
OrthoDBiEOG6CVV7G.

Family and domain databases

HAMAPiMF_01630. Nitrate_reduct.
InterProiIPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
IPR019546. TAT_signal_bac_arc.
[Graphical view]
PfamiPF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view]
SMARTiSM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view]
SUPFAMiSSF50692. SSF50692. 1 hit.
TIGRFAMsiTIGR01706. NAPA. 1 hit.
TIGR01409. TAT_signal_seq. 1 hit.
PROSITEiPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

B0URQ3-1 [UniParc]FASTAAdd to Basket

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        10         20         30         40         50
MNLSRRDFMK ANAALAAASV AGLIIPVKNV NAADTSITWD KAVCRFCGTG
60 70 80 90 100
CAVLVGTKDG RVVASQGDPD AEVNRGLNCI KGYFLPKIMY GKDRLTHPML
110 120 130 140 150
RMKNGQYDKE GEFTPVTWDF AFKTMAEKFK SALKAKGPNG VGMFTSGQST
160 170 180 190 200
IFEGVAKSKL FKAGLLSNNI DPNARHCMAS AAVAFVRTFG IDEPMGCYDD
210 220 230 240 250
IEHADAFVLW GSNMAEMHPI LWSRISDRRL ANPDTVSVNV LSTFEHRSFE
260 270 280 290 300
LADLGILLKP QSDLAILNYI ANYLIENNAI NREFIEKHTK FKRGETDIGY
310 320 330 340 350
GLRPQDPREQ TAKNVKTAGK MYDSSFEEFK KLVAPYTLEK AHEISGVPKE
360 370 380 390 400
QLEKLAKLYA DPNKKVVSYW TMGINQHTRG VWANHLIYNI HLLTGKISLP
410 420 430 440 450
GCGPFSLTGQ PSACGTAREV GTFIHRLPAD LVVIKPEHRK IAEKIWKLPE
460 470 480 490 500
GLISDKLGFH AVAQSRALKD GKMQVLWQMC NNNMQAGPNI NEETYPGWRN
510 520 530 540 550
PDNFIVVSDP YPTVSALSAD LILPTAMWVE KEGAYGNAER RTQFWRQQVK
560 570 580 590 600
APGEAKSDLW QLVEFSKYFT TDEVWPAEIL AKNPAYQGKT LYEVLYLNGQ
610 620 630 640 650
VNQYSNDELK GRLNDEAYHF GFYIQKGLFE EYASFGRGHG HDLADFDTYH
660 670 680 690 700
KARGLRWPVV DGKETLWRYR EGYDPYVKAG EGVSFYGQAD KRAVILAVPY
710 720 730 740 750
EPPAEVPDRK YDLWLTTGRI LEHWHTGSMT RRVPELHRSF PNNLVWMNPN
760 770 780 790 800
DAKKRGLKHG DKIKVISRRG EITSYIDTRG RNKCPEGLIY TTFFDAGQLA
810 820
NKLILDATDP ISKETDFKKC AVKVVKA
Length:827
Mass (Da):93,015
Last modified:April 8, 2008 - v1
Checksum:i161C8DCAACC85DEA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000947 Genomic DNA. Translation: ACA32139.1.
RefSeqiWP_012341329.1. NC_010519.1.
YP_001783837.1. NC_010519.1.

Genome annotation databases

EnsemblBacteriaiACA32139; ACA32139; HSM_0493.
GeneIDi6108282.
KEGGihsm:HSM_0493.
PATRICi20198017. VBIHaeSom93646_0513.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000947 Genomic DNA. Translation: ACA32139.1 .
RefSeqi WP_012341329.1. NC_010519.1.
YP_001783837.1. NC_010519.1.

3D structure databases

ProteinModelPortali B0URQ3.
SMRi B0URQ3. Positions 36-826.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 228400.HSM_0493.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACA32139 ; ACA32139 ; HSM_0493 .
GeneIDi 6108282.
KEGGi hsm:HSM_0493.
PATRICi 20198017. VBIHaeSom93646_0513.

Phylogenomic databases

eggNOGi COG0243.
HOGENOMi HOG000031441.
KOi K02567.
OrthoDBi EOG6CVV7G.

Enzyme and pathway databases

BioCyci HSOM228400:GHWT-505-MONOMER.

Family and domain databases

HAMAPi MF_01630. Nitrate_reduct.
InterProi IPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
IPR019546. TAT_signal_bac_arc.
[Graphical view ]
Pfami PF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view ]
SMARTi SM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view ]
SUPFAMi SSF50692. SSF50692. 1 hit.
TIGRFAMsi TIGR01706. NAPA. 1 hit.
TIGR01409. TAT_signal_seq. 1 hit.
PROSITEi PS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 2336.

Entry informationi

Entry nameiNAPA_HISS2
AccessioniPrimary (citable) accession number: B0URQ3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: April 8, 2008
Last modified: November 26, 2014
This is version 53 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3