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B0URQ3 (NAPA_HISS2) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 49. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Periplasmic nitrate reductase

EC=1.7.99.4
Gene names
Name:napA
Ordered Locus Names:HSM_0493
OrganismHistophilus somni (strain 2336) (Haemophilus somnus) [Complete proteome] [HAMAP]
Taxonomic identifier228400 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHistophilus

Protein attributes

Sequence length827 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein NapC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism By similarity. HAMAP-Rule MF_01630

Catalytic activity

Nitrite + acceptor = nitrate + reduced acceptor. HAMAP-Rule MF_01630

Cofactor

Binds 1 4Fe-4S cluster By similarity. HAMAP-Rule MF_01630

Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit By similarity.

Subunit structure

Interacts with NapB By similarity. HAMAP-Rule MF_01630

Subcellular location

Periplasm By similarity HAMAP-Rule MF_01630.

Post-translational modification

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven. HAMAP-Rule MF_01630

Sequence similarities

Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily.

Contains 1 4Fe-4S Mo/W bis-MGD-type domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3232Tat-type signal Potential
Chain33 – 827795Periplasmic nitrate reductase HAMAP-Rule MF_01630
PRO_5000310961

Regions

Domain37 – 93574Fe-4S Mo/W bis-MGD-type

Sites

Metal binding441Iron-sulfur (4Fe-4S) By similarity
Metal binding471Iron-sulfur (4Fe-4S) By similarity
Metal binding511Iron-sulfur (4Fe-4S) By similarity
Metal binding791Iron-sulfur (4Fe-4S) By similarity

Sequences

Sequence LengthMass (Da)Tools
B0URQ3 [UniParc].

Last modified April 8, 2008. Version 1.
Checksum: 161C8DCAACC85DEA

FASTA82793,015
        10         20         30         40         50         60 
MNLSRRDFMK ANAALAAASV AGLIIPVKNV NAADTSITWD KAVCRFCGTG CAVLVGTKDG 

        70         80         90        100        110        120 
RVVASQGDPD AEVNRGLNCI KGYFLPKIMY GKDRLTHPML RMKNGQYDKE GEFTPVTWDF 

       130        140        150        160        170        180 
AFKTMAEKFK SALKAKGPNG VGMFTSGQST IFEGVAKSKL FKAGLLSNNI DPNARHCMAS 

       190        200        210        220        230        240 
AAVAFVRTFG IDEPMGCYDD IEHADAFVLW GSNMAEMHPI LWSRISDRRL ANPDTVSVNV 

       250        260        270        280        290        300 
LSTFEHRSFE LADLGILLKP QSDLAILNYI ANYLIENNAI NREFIEKHTK FKRGETDIGY 

       310        320        330        340        350        360 
GLRPQDPREQ TAKNVKTAGK MYDSSFEEFK KLVAPYTLEK AHEISGVPKE QLEKLAKLYA 

       370        380        390        400        410        420 
DPNKKVVSYW TMGINQHTRG VWANHLIYNI HLLTGKISLP GCGPFSLTGQ PSACGTAREV 

       430        440        450        460        470        480 
GTFIHRLPAD LVVIKPEHRK IAEKIWKLPE GLISDKLGFH AVAQSRALKD GKMQVLWQMC 

       490        500        510        520        530        540 
NNNMQAGPNI NEETYPGWRN PDNFIVVSDP YPTVSALSAD LILPTAMWVE KEGAYGNAER 

       550        560        570        580        590        600 
RTQFWRQQVK APGEAKSDLW QLVEFSKYFT TDEVWPAEIL AKNPAYQGKT LYEVLYLNGQ 

       610        620        630        640        650        660 
VNQYSNDELK GRLNDEAYHF GFYIQKGLFE EYASFGRGHG HDLADFDTYH KARGLRWPVV 

       670        680        690        700        710        720 
DGKETLWRYR EGYDPYVKAG EGVSFYGQAD KRAVILAVPY EPPAEVPDRK YDLWLTTGRI 

       730        740        750        760        770        780 
LEHWHTGSMT RRVPELHRSF PNNLVWMNPN DAKKRGLKHG DKIKVISRRG EITSYIDTRG 

       790        800        810        820 
RNKCPEGLIY TTFFDAGQLA NKLILDATDP ISKETDFKKC AVKVVKA 

« Hide

References

[1]"Complete sequence of Haemophilus somnus 2336."
US DOE Joint Genome Institute
Siddaramappa S., Duncan A.J., Challacombe J.F., Rainey D., Gillaspy A.F., Carson M., Gipson J., Gipson M., Bruce D., Detter J.C., Han C.S., Land M., Tapia R., Thompson L.S., Orvis J., Zaitshik J., Barnes G., Brettin T.S., Dyer D.W., Inzana T.J.
Submitted (FEB-2008) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 2336.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000947 Genomic DNA. Translation: ACA32139.1.
RefSeqYP_001783837.1. NC_010519.1.

3D structure databases

ProteinModelPortalB0URQ3.
SMRB0URQ3. Positions 36-826.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING228400.HSM_0493.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACA32139; ACA32139; HSM_0493.
GeneID6108282.
KEGGhsm:HSM_0493.
PATRIC20198017. VBIHaeSom93646_0513.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0243.
HOGENOMHOG000031441.
KOK02567.
OMAFTTDEMW.
OrthoDBEOG6CVV7G.
ProtClustDBPRK13532.

Enzyme and pathway databases

BioCycHSOM228400:GHWT-505-MONOMER.

Family and domain databases

HAMAPMF_01630. Nitrate_reduct.
InterProIPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
IPR019546. TAT_signal_bac_arc.
[Graphical view]
PfamPF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view]
SMARTSM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view]
SUPFAMSSF50692. SSF50692. 1 hit.
TIGRFAMsTIGR01706. NAPA. 1 hit.
TIGR01409. TAT_signal_seq. 1 hit.
PROSITEPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameNAPA_HISS2
AccessionPrimary (citable) accession number: B0URQ3
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: April 8, 2008
Last modified: February 19, 2014
This is version 49 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families