B0UQX8 (B0UQX8_METS4) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
DNA gyrase subunit B RuleBase RU003363 HAMAP MF_01898
EC=5.99.1.3 RuleBase RU003363 HAMAP MF_01898

Gene names

Name:	gyrB HAMAP MF_01898
Ordered Locus Names:	M446_5333

Organism

Methylobacterium sp. (strain 4-46) [Complete proteome] [HAMAP]

Taxonomic identifier

426117 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Methylobacteriaceae › Methylobacterium

Protein attributes

Sequence length	808 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	DNA gyrase negatively supercoils closed circular double-stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings By similarity. RuleBase RU003363 HAMAP MF_01898
Catalytic activity	ATP-dependent breakage, passage and rejoining of double-stranded DNA. SAAS SAAS002288 RuleBase RU003363 HAMAP MF_01898
Subunit structure	Made up of two chains. The A chain is responsible for DNA breakage and rejoining; the B chain catalyzes ATP hydrolysis. The enzyme forms an A2B2 tetramer By similarity. HAMAP MF_01898
Subcellular location	Cytoplasm By similarity HAMAP MF_01898.
Sequence similarities	Belongs to the type II topoisomerase family. HAMAP MF_01898 RuleBase RU000380

Ontologies

Keywords
Cellular component	Cytoplasm HAMAP MF_01898
Ligand	ATP-binding SAAS SAAS002288 HAMAP MF_01898 RuleBase RU000380 Nucleotide-binding
Molecular function	Isomerase Topoisomerase SAAS SAAS002288 HAMAP MF_01898 RuleBase RU000380
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA topological change Inferred from electronic annotation. Source: InterPro DNA-dependent DNA replication Inferred from electronic annotation. Source: HAMAP
Cellular component	chromosome Inferred from electronic annotation. Source: InterPro cytoplasm Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP DNA topoisomerase (ATP-hydrolyzing) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	B0UQX8 [UniParc]. Last modified April 8, 2008. Version 1. Checksum: 90FA822BD1117776	FASTA	808	88,590
	10 20 30 40 50 60 MAEPARDLTP DTYGADSIKV LKGLDAVRKR PGMYIGDTDD GSGLHHMVYE VVDNAIDEAL 70 80 90 100 110 120 AGHATLVTVT LNPDGSCTVS DNGRGIPTDI HAEEGVSAAE VIMTQLHAGG KFDQNSYKVS 130 140 150 160 170 180 GGLHGVGVSV VNALSTWLRL RIWRGGQEHA MEFRHGDAVA PLAVVGPAGD RRGTEVTFLP 190 200 210 220 230 240 STETFSMVEF DYATLEKRLR ELAFLNSGVR IVLTDARHAE HKREELVYEG GVEAFVRYLD 250 260 270 280 290 300 RTKTPLVAKP VVVRAERDGI SVEVALWWND SYHETVLPFT NNIPQRDGGT HMAGFRAALT 310 320 330 340 350 360 RQITGYAESS GIAKKEKVTL TGDDCREGLT AIISVKVPDP KFSSQTKDKL VSSEVRPVVE 370 380 390 400 410 420 AVLGEGLSNW LEENPGEART IVGKVAEAAA AREAARKARE LTRRKGALDI ASLPGKLADC 430 440 450 460 470 480 QERDPSKCEI LLVEGDSAGG SAKQGRDRTF QAVLPLRGKI LNVERARFDK MLSSQEIGTL 490 500 510 520 530 540 ITALGTGIGR EEFNPDKLRY HRIIIMTDAD VDGSHIRTLL LTFFFRQMPE LIERGHLYIA 550 560 570 580 590 600 QPPLYKASKG RSHLYLKDER ALEDYLIDGG VEGASLRLAT GVAFAGPQLK ALVDEARGVR 610 620 630 640 650 660 TILHGLHTRY DRTVVEQAAI AGALRPALAE NPAEAEVVAD EVARRLNALS DEIERGWEGE 670 680 690 700 710 720 ARAGGYVLSR TLRGVRQVAV LDEALISSQE ARRLDERTDS LRQIYDAPVT LQRKGEEVVL 730 740 750 760 770 780 HGPVALFETT MALGRKGLQL QRYKGLGEMT AQQLWETTLD RDVRSLLQVK VKDTTDADDL 790 800 FVKLMGDVVE PRREFIQENA LNVANLDV

References

[1]

"Complete sequence of chromosome of Methylobacterium sp. 4-46." Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Chertkov O., Brettin T., Detter J.C., Han C., Kuske C.R., Schmutz J., Larimer F., Land M., Hauser L. , Kyrpides N., Ivanova N., Marx C.J., Richardson P. Submitted (FEB-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 4-46.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000943 Genomic DNA. Translation: ACA19651.1.
RefSeq	YP_001772085.1. NC_010511.1.
3D structure databases
ProteinModelPortal	B0UQX8.
SMR	B0UQX8. Positions 18-387.
ModBase	Search...
Protein-protein interaction databases
STRING	B0UQX8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6129530.
GenomeReviews	Gene locus M446_5333 in contig CP000943_GR.
KEGG	met:M446_5333.
PATRIC	22591878. VBIMetSp32184_5256.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG304889.
OMA	DCSSRDP.
ProtClustDB	PRK14939.
Family and domain databases
HAMAP	MF_01898. GyrB. [Tree]
InterPro	IPR003594. ATPase-like_ATP-bd. IPR011557. GyrB. IPR020568. Ribosomal_S5_D2-typ_fold. IPR014721. Ribosomal_S5_D2-typ_fold_subgr. IPR001241. Topo_IIA. IPR013759. Topo_IIA_B/N_ab. IPR002288. Topo_IIA_B_C. IPR013506. Topo_IIA_bsu_dom2. IPR013760. Topo_IIA_cen. IPR018522. TopoIIA_CS. [Graphical view]
Gene3D	G3DSA:3.30.565.10. ATP_bd_ATPase. 1 hit. G3DSA:3.30.230.10. Ribosomal_S5_D2-type_fold. 1 hit. G3DSA:3.40.50.670. Topo_IIA_B/N_ab. 2 hits.
KO	K02470.
Pfam	PF00204. DNA_gyraseB. 1 hit. PF00986. DNA_gyraseB_C. 1 hit. PF02518. HATPase_c. 1 hit. [Graphical view]
PRINTS	PR00418. TPI2FAMILY.
SMART	SM00387. HATPase_c. 1 hit. SM00433. TOP2c. 1 hit. [Graphical view]
SUPFAM	SSF55874. ATP_bd_ATPase. 1 hit. SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit. SSF56719. Topo_IIA_cen. 1 hit.
TIGRFAMs	TIGR01059. GyrB. 1 hit.
PROSITE	PS00177. TOPOISOMERASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

B0UQX8_METS4

Accession

Primary (citable) accession number: B0UQX8

Entry history

Integrated into UniProtKB/TrEMBL:	April 8, 2008
Last sequence update:	April 8, 2008
Last modified:	January 25, 2012
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)