Reviewed, UniProtKB/Swiss-Prot B0UQ09 (SYE1_METS4)

Names and origin

Protein names

Recommended name:
    Glutamyl-tRNA synthetase 1
    EC=6.1.1.17
Alternative name(s):
    Glutamate--tRNA ligase 1
      Short name=GluRS 1

Gene names

Name:	gltX1
Ordered Locus Names:	M446_0647

Organism

Methylobacterium sp. (strain 4-46) [Complete proteome] [HAMAP]

Taxonomic identifier

426117 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Methylobacteriaceae › Methylobacterium

Protein attributes

Sequence length	474 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity.
Catalytic activity	ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu). HAMAP MF_00022
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutamyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP glutamate-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 474	474	Glutamyl-tRNA synthetase 1 HAMAP MF_00022		PRO_0000367713
Regions
	Motif	10 – 20	11	"HIGH" region HAMAP MF_00022
	Motif	239 – 243	5	"KMSKS" region HAMAP MF_00022
Sites
	Binding site	242	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	B0UQ09-1 [UniParc]. Last modified April 8, 2008. Version 1. Checksum: 9DA2B3F05E7413FD	FASTA	474	52,159
	10 20 30 40 50 60 MSSVVTRFAP SPTGFLHIGG GRTALFNWLY ARKHGGRMLL RIEDTDRERS TEAAIEAILD 70 80 90 100 110 120 GLSWLGLDWD GDVIYQFARA ARHREVAEGL LAAGRAYRCY ASPEELTEMR EAARREGRPL 130 140 150 160 170 180 RYDGRWRDRN PSEAPPGVRP VIRLRAPVEG ETVVEDEVQG RVVWQNKDLD DLVLLRSDGT 190 200 210 220 230 240 PTYMLAVVVD DHDMGVTHVI RGDDHLTNAA RQTQIYHALG WTVPSMSHIP LIHGPDGAKL 250 260 270 280 290 300 SKRHGALGVD AYRGLGYLPA ALRNYLVRLG WSHGDQEVFS TEEMVAAFDL KQIGRSPARF 310 320 330 340 350 360 DFAKLENLNG QYIRATSDAD LVAAIEAILP SQGPARGLPA RFDDALRARF LAAMPGLKER 370 380 390 400 410 420 AKTLIELLDS AYYLYAPRPL ALDERAEGLL GNGGRERLAG VLPRLEALPD WSAASTEQAV 430 440 450 460 470 RDFAEAAAVK LGHVAQPLRA ALTGRATSPG LFDVMAVLGR EESLGRLRDQ ARAA

References

[1]

"Complete sequence of chromosome of Methylobacterium sp. 4-46." Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Chertkov O., Brettin T., Detter J.C., Han C., Kuske C.R., Schmutz J., Larimer F., Land M., Hauser L. , Kyrpides N., Ivanova N., Marx C.J., Richardson P. Submitted (FEB-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000943 Genomic DNA. Translation: ACA15208.1.
RefSeq	YP_001767642.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	6135362.
GenomeReviews	Gene locus M446_0647 in contig CP000943_GR.
KEGG	met:M446_0647.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	MAHIPLI.
Family and domain databases
HAMAP	MF_00022. [Tree]
InterPro	IPR008925. aa-tRNA-synth_I_codon-bd. IPR001412. aa-tRNA-synth_I_CS. IPR004527. Glu-tRNA-synth_Ic_bac/mito. IPR000924. Glu/Gln-tRNA-synth_Ic. IPR020061. Glu/Gln-tRNA-synth_Ic_a-bdl. IPR020058. Glu/Gln-tRNA-synth_Ic_cat-dom. IPR020060. Glu/Gln-tRNA-synth_Ic_N. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:1.10.1160.10. Glu/Gln-tRNA-synth_Ic_a-bdl. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit. G3DSA:1.10.10.350. tRNA_synt_bd. 1 hit.
PANTHER	PTHR10119. Glu_tRNA-synt_1c. 1 hit.
Pfam	PF00749. tRNA-synt_1c. 1 hit. [Graphical view]
PRINTS	PR00987. TRNASYNTHGLU.
TIGRFAMs	TIGR00464. gltX_bact. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYE1_METS4

Accession

Primary (citable) accession number: B0UQ09

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	April 8, 2008
Last modified:	November 3, 2009
This is version 12 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)