B0UQ03 (LPXD_METS4) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
UDP-3-O-acylglucosamine N-acyltransferase
EC=2.3.1.-

Gene names

Name:	lpxD
Ordered Locus Names:	M446_0641

Organism

Methylobacterium sp. (strain 4-46) [Complete proteome] [HAMAP]

Taxonomic identifier

426117 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Methylobacteriaceae › Methylobacterium

Protein attributes

Sequence length	352 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the N-acylation of UDP-3-O-acylglucosamine using 3-hydroxyacyl-ACP as the acyl donor. Is involved in the biosynthesis of lipid A, a phosphorylated glycolipid that anchors the lipopolysaccharide to the outer membrane of the cell By similarity. HAMAP MF_00523
Catalytic activity	(3R)-3-hydroxyacyl-[acyl-carrier-protein] + UDP-3-O-acyl-alpha-D-glucosamine = UDP-2,3-diacyl-alpha-D-glucosamine + [acyl-carrier-protein]. HAMAP MF_00523
Pathway	Bacterial outer membrane biogenesis; LPS lipid A biosynthesis. HAMAP MF_00523
Subunit structure	Homotrimer By similarity. HAMAP MF_00523
Sequence similarities	Belongs to the transferase hexapeptide repeat family. LpxD subfamily.

Ontologies

Keywords
Biological process	Lipid A biosynthesis Lipid synthesis
Domain	Repeat
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	lipid A biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	transferase activity, transferring acyl groups other than amino-acyl groups Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 352	352	UDP-3-O-acylglucosamine N-acyltransferase HAMAP MF_00523		PRO_1000127687
Sites
	Active site	257	1	Proton acceptor By similarity

Sequences

	Sequence		Length	Mass (Da)
	B0UQ03 [UniParc]. Last modified April 8, 2008. Version 1. Checksum: FD75438229F50EA0	FASTA	352	35,625
	10 20 30 40 50 60 MSDPVFFPFA GPISLGEVAA LAGAALPAGL DGTLAVAGAA PLESAGPDDL AYMDNAKYAE 70 80 90 100 110 120 ALGRTRARAC LVSPRFAARV PEGTAALVTP QPYRGFAQVL ARLFPSAARP GSLFGATGVS 130 140 150 160 170 180 PGSFVHPEAR LEPGVVVDPG VVIGPGAEIG SGTVLAAGAV VGPGTRIGRG CAIGPGASLL 190 200 210 220 230 240 HALVGNRVIV HGGARIGQDG FGFAMGAGGH LKVPQVGRVI IQDDVEIGAN TTIDRGASRD 250 260 270 280 290 300 TIVGEGTKID NLVQIAHNVV IGRHCVIVAQ VGISGSTTLE DYVVLGGQVG VVGHLRIGMG 310 320 330 340 350 AQIAGSSNIN KDVPPGARWG GTPAKPVREW FREMTTLKRL AARERGPEDT DG

References

[1]

"Complete sequence of chromosome of Methylobacterium sp. 4-46." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Chertkov O., Brettin T., Detter J.C., Han C., Kuske C.R., Schmutz J., Larimer F., Land M., Hauser L. , Kyrpides N., Ivanova N., Marx C.J., Richardson P. Submitted (FEB-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 4-46.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000943 Genomic DNA. Translation: ACA15202.1.
RefSeq	YP_001767636.1. NC_010511.1.
3D structure databases
ProteinModelPortal	B0UQ03.
ModBase	Search...
Protein-protein interaction databases
STRING	B0UQ03.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6135356.
GenomeReviews	Gene locus M446_0641 in contig CP000943_GR.
KEGG	met:M446_0641.
PATRIC	22582713. VBIMetSp32184_0725.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG469615.
OMA	CFVGKNT.
ProtClustDB	PRK00892.
Enzyme and pathway databases
BioCyc	MSP409:M446_0641-MONOMER.
Family and domain databases
HAMAP	MF_00523. LpxD. [Tree]
InterPro	IPR001451. Hexapep_transf. IPR018357. Hexapep_transf_CS. IPR011004. Trimer_LpxA-like. IPR007691. UDP-3-O_GlcNAc_AcTrfase. IPR020573. UDP_GlcNAc_AcTrfase_non-rep. [Graphical view]
KO	K02536.
Pfam	PF00132. Hexapep. 3 hits. PF04613. LpxD. 1 hit. [Graphical view]
SUPFAM	SSF51161. Trimer_LpxA_like. 1 hit.
TIGRFAMs	TIGR01853. Lipid_A_lpxD. 1 hit.
PROSITE	PS00101. HEXAPEP_TRANSFERASES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LPXD_METS4

Accession

Primary (citable) accession number: B0UQ03

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	April 8, 2008
Last modified:	January 25, 2012
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program