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Protein

tRNA dimethylallyltransferase

Gene

miaA

Organism
Methylobacterium sp. (strain 4-46)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i6A).UniRule annotation

Catalytic activityi

Dimethylallyl diphosphate + adenine(37) in tRNA = diphosphate + N(6)-dimethylallyladenine(37) in tRNA.UniRule annotation

Cofactori

Mg2+UniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi15 – 228ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

ATP-binding, Magnesium, Nucleotide-binding

Enzyme and pathway databases

BioCyciMSP426117:GI2I-6258-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA dimethylallyltransferaseUniRule annotation (EC:2.5.1.75UniRule annotation)
Alternative name(s):
Dimethylallyl diphosphate:tRNA dimethylallyltransferaseUniRule annotation
Short name:
DMAPP:tRNA dimethylallyltransferaseUniRule annotation
Short name:
DMATaseUniRule annotation
Isopentenyl-diphosphate:tRNA isopentenyltransferaseUniRule annotation
Short name:
IPP transferaseUniRule annotation
Short name:
IPPTUniRule annotation
Short name:
IPTaseUniRule annotation
Gene namesi
Name:miaAUniRule annotation
Ordered Locus Names:M446_6189
OrganismiMethylobacterium sp. (strain 4-46)
Taxonomic identifieri426117 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesMethylobacteriaceaeMethylobacterium
Proteomesi
  • UP000001185 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 306306tRNA dimethylallyltransferasePRO_0000377221Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei106 – 1061Interaction with substrate tRNAUniRule annotation
Sitei128 – 1281Interaction with substrate tRNAUniRule annotation

Protein-protein interaction databases

STRINGi426117.M446_6189.

Structurei

3D structure databases

ProteinModelPortaliB0UPN1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni17 – 226Substrate bindingUniRule annotation
Regioni40 – 434Interaction with substrate tRNAUniRule annotation

Sequence similaritiesi

Belongs to the IPP transferase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105DKX. Bacteria.
COG0324. LUCA.
HOGENOMiHOG000039996.
KOiK00791.
OMAiTWFRNQT.
OrthoDBiPOG091H00NK.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00185. IPP_trans. 1 hit.
InterProiIPR018022. IPP_trans.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF01715. IPPT. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 3 hits.
TIGRFAMsiTIGR00174. miaA. 1 hit.

Sequencei

Sequence statusi: Complete.

B0UPN1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGTDGMAGRA ILIAGPTASG KSALALALAR ARGGVVINAD SMQVYADLRV
60 70 80 90 100
LTARPNPAEE AQAPHRLYGS VDGAVNFSVG HYLAAVGEVL REVWAAGGLP
110 120 130 140 150
IVVGGTGLYF KALLEGLSEI PPVPEAVRTA LRAEAEGRET AALHADLARR
160 170 180 190 200
DPEGAARLGA HDRLRVLRAL EVLAATGRPL SAFQGSRRPG PLAGMPCDKL
210 220 230 240 250
FLVPDRALLR ARIDARFLAM MEEGALDEVA RLRARRLDPM LPVMRAHGVP
260 270 280 290 300
GLIAFLDGAL TREEAVARGQ ADTRAYAKRQ VTWFRHQAGA GWRWLAPEAA

MREAGG
Length:306
Mass (Da):32,491
Last modified:April 8, 2008 - v1
Checksum:i3F401A2EEF1F611E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000943 Genomic DNA. Translation: ACA20457.1.
RefSeqiWP_012335835.1. NC_010511.1.

Genome annotation databases

EnsemblBacteriaiACA20457; ACA20457; M446_6189.
KEGGimet:M446_6189.
PATRICi22593655. VBIMetSp32184_6134.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000943 Genomic DNA. Translation: ACA20457.1.
RefSeqiWP_012335835.1. NC_010511.1.

3D structure databases

ProteinModelPortaliB0UPN1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi426117.M446_6189.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACA20457; ACA20457; M446_6189.
KEGGimet:M446_6189.
PATRICi22593655. VBIMetSp32184_6134.

Phylogenomic databases

eggNOGiENOG4105DKX. Bacteria.
COG0324. LUCA.
HOGENOMiHOG000039996.
KOiK00791.
OMAiTWFRNQT.
OrthoDBiPOG091H00NK.

Enzyme and pathway databases

BioCyciMSP426117:GI2I-6258-MONOMER.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00185. IPP_trans. 1 hit.
InterProiIPR018022. IPP_trans.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF01715. IPPT. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 3 hits.
TIGRFAMsiTIGR00174. miaA. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMIAA_METS4
AccessioniPrimary (citable) accession number: B0UPN1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 16, 2009
Last sequence update: April 8, 2008
Last modified: September 7, 2016
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.