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B0UP40 (TRUA_METS4) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 45. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA pseudouridine synthase A

EC=5.4.99.12
Alternative name(s):
tRNA pseudouridine(38-40) synthase
tRNA pseudouridylate synthase I
tRNA-uridine isomerase I
Gene names
Name:truA
Ordered Locus Names:M446_4238
OrganismMethylobacterium sp. (strain 4-46) [Complete proteome] [HAMAP]
Taxonomic identifier426117 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesMethylobacteriaceaeMethylobacterium

Protein attributes

Sequence length250 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs By similarity. HAMAP-Rule MF_00171

Catalytic activity

tRNA uridine(38-40) = tRNA pseudouridine(38-40). HAMAP-Rule MF_00171

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_00171

Sequence similarities

Belongs to the tRNA pseudouridine synthase TruA family.

Ontologies

Keywords
   Biological processtRNA processing
   Molecular functionIsomerase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processtRNA pseudouridine synthesis

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Molecular_functionRNA binding

Inferred from electronic annotation. Source: InterPro

pseudouridine synthase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 250250tRNA pseudouridine synthase A HAMAP-Rule MF_00171
PRO_1000097763

Sites

Active site521Nucleophile By similarity
Binding site1111Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
B0UP40 [UniParc].

Last modified April 8, 2008. Version 1.
Checksum: 626195D8767B5600

FASTA25027,525
        10         20         30         40         50         60 
MPRYKLVVEY DGSGFAGWQR QAADRTVQQA LEEAIARFAG APVRVHCAGR TDAGVHATHQ 

        70         80         90        100        110        120 
VVHLDLDRDW RTDTVRDASN AHLRPEPVAV VSAERVRPDF DARHSALRRH YRYRILNRRS 

       130        140        150        160        170        180 
PPALGRAYLW HVPWSLDAHA MHAAAQTLLG RHDFSAFRAA ECQANSPVRT LDQLDVARIG 

       190        200        210        220        230        240 
DEILVATSAR SFLHHQVRGM VGTLMLAGCG RLDAAGVRAV LDSRDRTRCG PLAPAAGLTL 

       250 
TGVDYPEPGP 

« Hide

References

[1]"Complete sequence of chromosome of Methylobacterium sp. 4-46."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Chertkov O., Brettin T., Detter J.C., Han C., Kuske C.R., Schmutz J., Larimer F., Land M., Hauser L. expand/collapse author list , Kyrpides N., Ivanova N., Marx C.J., Richardson P.
Submitted (FEB-2008) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 4-46.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000943 Genomic DNA. Translation: ACA18587.1.
RefSeqYP_001771021.1. NC_010511.1.

3D structure databases

ProteinModelPortalB0UP40.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING426117.M446_4238.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACA18587; ACA18587; M446_4238.
GeneID6129982.
KEGGmet:M446_4238.
PATRIC22589646. VBIMetSp32184_4157.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0101.
HOGENOMHOG000248674.
KOK06173.
OMAWDPFRLS.
OrthoDBEOG6Z9B4R.

Enzyme and pathway databases

BioCycMSP426117:GI2I-4279-MONOMER.

Family and domain databases

Gene3D3.30.70.580. 1 hit.
3.30.70.660. 1 hit.
HAMAPMF_00171. TruA.
InterProIPR020103. PsdUridine_synth_cat_dom.
IPR001406. PsdUridine_synth_TruA.
IPR020097. PsdUridine_synth_TruA_a/b_dom.
IPR020095. PsdUridine_synth_TruA_C.
IPR020094. PsdUridine_synth_TruA_N.
[Graphical view]
PANTHERPTHR11142. PTHR11142. 1 hit.
PfamPF01416. PseudoU_synth_1. 2 hits.
[Graphical view]
PIRSFPIRSF001430. tRNA_psdUrid_synth. 1 hit.
SUPFAMSSF55120. SSF55120. 1 hit.
TIGRFAMsTIGR00071. hisT_truA. 1 hit.
ProtoNetSearch...

Entry information

Entry nameTRUA_METS4
AccessionPrimary (citable) accession number: B0UP40
Entry history
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: April 8, 2008
Last modified: May 14, 2014
This is version 45 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families