B0UNT1 (B0UNT1_METS4) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
4-diphosphocytidyl-2-C-methyl-D-erythritol kinase HAMAP MF_00061
Short name=CMK HAMAP MF_00061
EC=2.7.1.148 HAMAP MF_00061

Alternative name(s):
4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase HAMAP MF_00061

Gene names

Name:	ispE HAMAP MF_00061
Ordered Locus Names:	M446_2748

Organism

Methylobacterium sp. (strain 4-46) [Complete proteome] [HAMAP]

Taxonomic identifier

426117 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Methylobacteriaceae › Methylobacterium

Protein attributes

Sequence length	305 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol By similarity. HAMAP MF_00061
Catalytic activity	ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol. HAMAP MF_00061
Pathway	Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 3/6. HAMAP MF_00061
Sequence similarities	Belongs to the GHMP kinase family. IspE subfamily. HAMAP MF_00061

Ontologies

Keywords
Biological process	Isoprene biosynthesis HAMAP MF_00061
Ligand	ATP-binding HAMAP MF_00061 RuleBase RU003563 Nucleotide-binding
Molecular function	Kinase HAMAP MF_00061 RuleBase RU003563 EMBL ACA17186.1 Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	terpenoid biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase activity Inferred from electronic annotation. Source: HAMAP ATP binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Regions
	Nucleotide binding	109 – 119	11	ATP By similarity HAMAP MF_00061
Sites
	Active site	23	1	By similarity HAMAP MF_00061
	Active site	151	1	By similarity HAMAP MF_00061

Sequences

	Sequence		Length	Mass (Da)
	B0UNT1 [UniParc]. Last modified April 8, 2008. Version 1. Checksum: EAB545E3A8B6C64E	FASTA	305	30,875
	10 20 30 40 50 60 MTSPTTCPTT CPTTCLTTRA PAKINLTLHV LGRRPEDGYH ALESLVAFAG VGDTLTLEPG 70 80 90 100 110 120 PDLALVVSGP TAGPAGPTAD NLVLRAARRL AALVPGLATG RFSLVKRLPV AAGIGGGSSD 130 140 150 160 170 180 AAAALRLLGR LNDLPADHPA LIEAARGTGA DVPVCLDPRA RMMRGAGEAI GPALALPGLP 190 200 210 220 230 240 AVLINPGVPV ETAPVFRALG LKVGEANPAA SVPHPTIGPR LDGPALLALI GPARNDLEAP 250 260 270 280 290 300 ALTVAPVIGE ALGALRERPG CRLARMSGSG ATVFGLFGHR GRAAQAARAI AQARPGWWVR ATILR

References

[1]

"Complete sequence of chromosome of Methylobacterium sp. 4-46." Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Chertkov O., Brettin T., Detter J.C., Han C., Kuske C.R., Schmutz J., Larimer F., Land M., Hauser L. , Kyrpides N., Ivanova N., Marx C.J., Richardson P. Submitted (FEB-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 4-46.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000943 Genomic DNA. Translation: ACA17186.1.
RefSeq	YP_001769620.1. NC_010511.1.
3D structure databases
ProteinModelPortal	B0UNT1.
ModBase	Search...
Protein-protein interaction databases
STRING	B0UNT1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6128958.
GenomeReviews	Gene locus M446_2748 in contig CP000943_GR.
KEGG	met:M446_2748.
PATRIC	22586768. VBIMetSp32184_2736.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG734593.
OMA	CQLARMS.
ProtClustDB	PRK14608.
Family and domain databases
HAMAP	MF_00061. IspE. [Tree]
InterPro	IPR006204. GHMP_kinase. IPR013750. GHMP_kinase_C. IPR004424. IspE. IPR020568. Ribosomal_S5_D2-typ_fold. IPR014721. Ribosomal_S5_D2-typ_fold_subgr. [Graphical view]
Gene3D	G3DSA:3.30.230.10. Ribosomal_S5_D2-type_fold. 1 hit.
KO	K00919.
PANTHER	PTHR20861:SF2. IspE. 1 hit.
Pfam	PF08544. GHMP_kinases_C. 1 hit. PF00288. GHMP_kinases_N. 1 hit. [Graphical view]
PIRSF	PIRSF010376. IspE. 1 hit.
SUPFAM	SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit.
ProtoNet	Search...

Entry information

Entry name

B0UNT1_METS4

Accession

Primary (citable) accession number: B0UNT1

Entry history

Integrated into UniProtKB/TrEMBL:	April 8, 2008
Last sequence update:	April 8, 2008
Last modified:	December 14, 2011
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)