B0UMS9 (PROA_METS4) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Gamma-glutamyl phosphate reductase
Short name=GPR
EC=1.2.1.41

Alternative name(s):
Glutamate-5-semialdehyde dehydrogenase
Glutamyl-gamma-semialdehyde dehydrogenase
Short name=GSA dehydrogenase

Gene names

Name:	proA
Ordered Locus Names:	M446_4218

Organism

Methylobacterium sp. (strain 4-46) [Complete proteome] [HAMAP]

Taxonomic identifier

426117 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Methylobacteriaceae › Methylobacterium

Protein attributes

Sequence length	431 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the NADPH dependent reduction of L-gamma-glutamyl 5-phosphate into L-glutamate 5-semialdehyde and phosphate. The product spontaneously undergoes cyclization to form 1-pyrroline-5-carboxylate By similarity. HAMAP MF_00412
Catalytic activity	L-glutamate 5-semialdehyde + phosphate + NADP+ = L-glutamyl 5-phosphate + NADPH. HAMAP MF_00412
Pathway	Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 2/2. HAMAP MF_00412
Subcellular location	Cytoplasm By similarity HAMAP MF_00412.
Sequence similarities	Belongs to the gamma-glutamyl phosphate reductase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Proline biosynthesis
Cellular component	Cytoplasm
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proline biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NADP binding Inferred from electronic annotation. Source: InterPro glutamate-5-semialdehyde dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 431	431	Gamma-glutamyl phosphate reductase HAMAP MF_00412		PRO_0000340895

Sequences

	Sequence		Length	Mass (Da)
	B0UMS9 [UniParc]. Last modified April 8, 2008. Version 1. Checksum: 0B4F622D081CCA6D	FASTA	431	44,656
	10 20 30 40 50 60 MSVLSLKPRA GADDVDALMR EIGRRARAAS RRMALVPARA KDMALRAAAA AIRDAAPVIL 70 80 90 100 110 120 EANAADLAEA RGANLPAATL DRLALTPGRV EAIAAAVEAI AGLPDPVGRQ LAAFERPNGL 130 140 150 160 170 180 AIERISTPLG VVGVIYESRP NVTADAGALC LKAGNAAVLR AGSESLRSAA AIARAMADGL 190 200 210 220 230 240 AAQGLPAEAI QLVPTRDRAA VGAMLAGLDG CIDVIVPRGG KSLVARVQSE ARVPVFAHLE 250 260 270 280 290 300 GICHVFVHAR ADLAMAREIL RNSKLRRTGI CGAAETLLVD RACAGTHLAP LVADLLEAGC 310 320 330 340 350 360 AVRGDAETQA VDPRVTPATE ADWRTEYLDA VIAVRVVDGL DAAIDHVETY GSHHTDAIVT 370 380 390 400 410 420 ADEAAAERFL AEVDSAIVVH NASTQFADGG EFGFGAEIGI ATGRMHARGP VGVEQLTTFK 430 YRVHGSGQVR P

References

[1]

"Complete sequence of chromosome of Methylobacterium sp. 4-46." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Chertkov O., Brettin T., Detter J.C., Han C., Kuske C.R., Schmutz J., Larimer F., Land M., Hauser L. , Kyrpides N., Ivanova N., Marx C.J., Richardson P. Submitted (FEB-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 4-46.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000943 Genomic DNA. Translation: ACA18567.1.
RefSeq	YP_001771001.1. NC_010511.1.
3D structure databases
ProteinModelPortal	B0UMS9.
ModBase	Search...
Protein-protein interaction databases
STRING	B0UMS9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6135420.
GenomeReviews	Gene locus M446_4218 in contig CP000943_GR.
KEGG	met:M446_4218.
PATRIC	22589604. VBIMetSp32184_4136.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG318080.
OMA	YGICGAM.
ProtClustDB	PRK00197.
Enzyme and pathway databases
BioCyc	MSP409:M446_4218-MONOMER.
Family and domain databases
HAMAP	MF_00412. ProA. [Tree]
InterPro	IPR016161. Ald_DH/histidinol_DH. IPR016163. Ald_DH_C. IPR016162. Ald_DH_N. IPR015590. Aldehyde_DH_dom. IPR000965. G-glutamylP_reductase. IPR012134. Glu-5-SA_DH. [Graphical view]
Gene3D	G3DSA:3.40.309.10. Aldehyde_dehydrogenase_C. 1 hit. G3DSA:3.40.605.10. Aldehyde_dehydrogenase_N. 2 hits.
KO	K00147.
PANTHER	PTHR11063:SF1. GSA_DH. 1 hit.
Pfam	PF00171. Aldedh. 1 hit. [Graphical view]
PIRSF	PIRSF000151. GPR. 1 hit.
SUPFAM	SSF53720. Aldehyde_DH/Histidinol_DH. 1 hit.
TIGRFAMs	TIGR00407. ProA. 1 hit.
PROSITE	PS01223. PROA. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PROA_METS4

Accession

Primary (citable) accession number: B0UMS9

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 10, 2008
Last sequence update:	April 8, 2008
Last modified:	January 25, 2012
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program