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Protein

8-amino-7-oxononanoate synthase

Gene

M446_5168

Organism
Methylobacterium sp. (strain 4-46)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the decarboxylative condensation of pimeloyl-[acyl-carrier protein] and L-alanine to produce 8-amino-7-oxononanoate (AON), [acyl-carrier protein], and carbon dioxide.By similarity

Catalytic activityi

Pimeloyl-[acyl-carrier protein] + L-alanine = 8-amino-7-oxononanoate + CO2 + holo-[acyl-carrier protein].

Cofactori

Pathway: biotin biosynthesis

This protein is involved in the pathway biotin biosynthesis, which is part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the pathway biotin biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei27 – 271SubstrateBy similarity
Binding sitei34 – 341SubstrateBy similarity
Binding sitei139 – 1391SubstrateBy similarity
Binding sitei187 – 1871Pyridoxal phosphateBy similarity
Binding sitei344 – 3441SubstrateBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Biological processi

Biotin biosynthesis

Keywords - Ligandi

Pyridoxal phosphate

Enzyme and pathway databases

BioCyciMSP426117:GI2I-5223-MONOMER.
UniPathwayiUPA00078.

Names & Taxonomyi

Protein namesi
Recommended name:
8-amino-7-oxononanoate synthase (EC:2.3.1.47)
Short name:
AONS
Alternative name(s):
7-keto-8-amino-pelargonic acid synthase
Short name:
7-KAP synthase
Short name:
KAPA synthase
8-amino-7-ketopelargonate synthase
Alpha-oxoamine synthase
Gene namesi
Ordered Locus Names:M446_5168
OrganismiMethylobacterium sp. (strain 4-46)
Taxonomic identifieri426117 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesMethylobacteriaceaeMethylobacterium
ProteomesiUP000001185 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 3793798-amino-7-oxononanoate synthasePRO_0000381029Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei235 – 2351N6-(pyridoxal phosphate)lysineBy similarity

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi426117.M446_5168.

Structurei

3D structure databases

ProteinModelPortaliB0UKC8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni114 – 1152Pyridoxal phosphate bindingBy similarity
Regioni212 – 2154Pyridoxal phosphate bindingBy similarity
Regioni232 – 2354Pyridoxal phosphate bindingBy similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0156.
HOGENOMiHOG000221021.
KOiK00652.
OMAiFEDRLNH.
OrthoDBiEOG6Q8HZD.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProiIPR001917. Aminotrans_II_pyridoxalP_BS.
IPR004839. Aminotransferase_I/II.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PfamiPF00155. Aminotran_1_2. 1 hit.
[Graphical view]
SUPFAMiSSF53383. SSF53383. 1 hit.
PROSITEiPS00599. AA_TRANSFER_CLASS_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B0UKC8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPVPPTLSLD AFAAAKLAGL DAAGLRRRLV PTARTGGARA ERDGRAVVSF
60 70 80 90 100
SCNDYLGLAT HPEVVAAAHA ALDRYGAGSG GSRLVTGSHP ILAELEAALA
110 120 130 140 150
ARKGHEAALV FGSGYLANLG VTPALVGAGD LILIDELGHS CMWAGTRLAG
160 170 180 190 200
ARALPFRHND LGHLEDLLAR ERARARRALI LTERVFSMDG DRAPVAEILG
210 220 230 240 250
LARAFDAWTL VDDAHGLGVV GPDATAPLEM GTLSKALGSY GGYLCASRPV
260 270 280 290 300
IDLLTSRARS FVYTTGLPPA SAAAALAALR LIEAEPARAA RPLALARRFT
310 320 330 340 350
ARLGLPEAQS AVVPVLVGEA EAALALSRAL EARGFLVVAI RPPTVPPGTA
360 370
RLRVAFSAAH EEAEVDALAQ ALLDLGAAA
Length:379
Mass (Da):39,067
Last modified:April 8, 2008 - v1
Checksum:i3700381E5CD27219
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000943 Genomic DNA. Translation: ACA19493.1.
RefSeqiWP_012334879.1. NC_010511.1.
YP_001771927.1. NC_010511.1.

Genome annotation databases

EnsemblBacteriaiACA19493; ACA19493; M446_5168.
KEGGimet:M446_5168.
PATRICi22591526. VBIMetSp32184_5083.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000943 Genomic DNA. Translation: ACA19493.1.
RefSeqiWP_012334879.1. NC_010511.1.
YP_001771927.1. NC_010511.1.

3D structure databases

ProteinModelPortaliB0UKC8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi426117.M446_5168.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACA19493; ACA19493; M446_5168.
KEGGimet:M446_5168.
PATRICi22591526. VBIMetSp32184_5083.

Phylogenomic databases

eggNOGiCOG0156.
HOGENOMiHOG000221021.
KOiK00652.
OMAiFEDRLNH.
OrthoDBiEOG6Q8HZD.

Enzyme and pathway databases

UniPathwayiUPA00078.
BioCyciMSP426117:GI2I-5223-MONOMER.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProiIPR001917. Aminotrans_II_pyridoxalP_BS.
IPR004839. Aminotransferase_I/II.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PfamiPF00155. Aminotran_1_2. 1 hit.
[Graphical view]
SUPFAMiSSF53383. SSF53383. 1 hit.
PROSITEiPS00599. AA_TRANSFER_CLASS_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 4-46.

Entry informationi

Entry nameiBIOF_METS4
AccessioniPrimary (citable) accession number: B0UKC8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: April 8, 2008
Last modified: April 29, 2015
This is version 50 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.